LAR protein tyrosine phosphatase regulates focal adhesions through CDK1.

abstract：:The mechanism responsible for spermatid translocation in the mammalian seminiferous epithelium was proposed to be the microtubule-based transport of specialized junction plaques (ectoplasmic specializations) that occur in Sertoli cell regions attached to spermatid heads. These plaques each consist of a cistern of endo...

journal_title：Journal of cell science

authors： Guttman JA,Kimel GH,Vogl AW

更新日期：2000-06-01 00:00:00

abstract：:PINCH1 is composed of 5 LIM domains, binds integrin-linked kinase (ILK) and locates to integrin-mediated adhesion sites. In order to investigate PINCH1 function we generated mice and embryonic stem (ES) cell-derived embryoid bodies (EBs) lacking the PINCH1 gene. Similar to mice lacking beta1 integrin or Ilk, loss of P...

journal_title：Journal of cell science

authors： Li S,Bordoy R,Stanchi F,Moser M,Braun A,Kudlacek O,Wewer UM,Yurchenco PD,Fässler R

更新日期：2005-07-01 00:00:00

abstract：:Several properties of cadherin-4 and cadherin-5 were characterized by using the cDNA transfection approach. The proteins of both cadherins had a relative molecular mass of about 130 kDa and were present at the cell periphery, especially at cell-cell contact sites. These cadherins were easily digested with trypsin, and...

journal_title：Journal of cell science

authors： Tanihara H,Kido M,Obata S,Heimark RL,Davidson M,St John T,Suzuki S

更新日期：1994-06-01 00:00:00

abstract：:Protein with tau-like repeats (PTL-1) is the sole Caenorhabditis elegans homolog of tau and MAP2, which are members of the mammalian family of microtubule-associated proteins (MAPs). In mammalian neurons, tau and MAP2 are segregated, with tau being mainly localised to the axon and MAP2 mainly to the dendrite. In parti...

journal_title：Journal of cell science

authors： Chew YL,Fan X,Götz J,Nicholas HR

更新日期：2013-05-01 00:00:00

abstract：:MAP1B is a developmentally regulated microtubule-associated phosphoprotein that regulates microtubule dynamics in growing axons and growth cones. We used mass spectrometry to map 28 phosphorylation sites on MAP1B, and selected for further study a putative primed GSK3 beta site and compared it with two nonprimed GSK3 b...

journal_title：Journal of cell science

authors： Scales TM,Lin S,Kraus M,Goold RG,Gordon-Weeks PR

更新日期：2009-07-15 00:00:00

abstract：:Polyethylene glycol (PEG) was used to fuse trout sperm with Chinese hamster fibroblasts (CHW-1102). As judged by light microscopy, PEG significantly increased the number of associations between the two cell types. Electron microscopy revealed that the sperm nuclei were in the cytoplasm of CHW-1102 in cultures that had...

journal_title：Journal of cell science

authors： Yip JH,Bols NC

更新日期：1982-02-01 00:00:00

abstract：:Phosphatidylinositol-3-phosphate [PtdIns(3)P] regulates endocytic and autophagic membrane traffic. In order to understand the downstream effects of PtdIns(3)P in these processes, it is important to identify PtdIns(3)P-binding proteins, many of which contain FYVE zinc-finger domains. Here, we describe a novel giant FYV...

journal_title：Journal of cell science

authors： Simonsen A,Birkeland HC,Gillooly DJ,Mizushima N,Kuma A,Yoshimori T,Slagsvold T,Brech A,Stenmark H

更新日期：2004-08-15 00:00:00

abstract：:Kit ligand (Kitl), the ligand for the Kit receptor tyrosine kinase, plays important roles in hematopoiesis, gametogenesis and melanogenesis. Kitl is synthesized as a membrane-anchored precursor that can be processed to produce the soluble growth factor. Here, we evaluated the role of ADAM (a disintegrin and metallopro...

journal_title：Journal of cell science

authors： Kawaguchi N,Horiuchi K,Becherer JD,Toyama Y,Besmer P,Blobel CP

更新日期：2007-03-15 00:00:00

abstract：:It remains unclear how GPI-anchored proteins (GPIAPs), which lack cytoplasmic domains, transduce signals triggered by specific ligation. Such signal transduction has been speculated to require the ligated GPIAP to associate with membrane-spanning proteins that communicate with obligate cytoplasmic proteins. Transient ...

journal_title：Journal of cell science

authors： Chen Y,Veracini L,Benistant C,Jacobson K

更新日期：2009-11-01 00:00:00

abstract：:Gap junctions (GJs) assembled from connexin (Cx) proteins allow direct cell-cell communication. While phosphorylation is known to regulate multiple GJ functions, much less is known about the role of ubiquitin in these processes. Using ubiquitylation-type-specific antibodies and Cx43 lysine-to-arginine mutants we show ...

journal_title：Journal of cell science

authors： Kells-Andrews RM,Margraf RA,Fisher CG,Falk MM

更新日期：2018-08-09 00:00:00

abstract：:Members of the bimC family of kinesin related proteins (KRPs) play vital roles in the formation and function of the mitotic spindle. Although they share little amino acid homology outside the highly conserved microtubule motor domain, several family members do contain a 'bimC box', a sequence motif around a p34(cdc2) ...

journal_title：Journal of cell science

authors： Drummond DR,Hagan IM

更新日期：1998-04-01 00:00:00

abstract：:Cadherins and integrins are intrinsically linked through the actin cytoskeleton and share common signaling molecules. Although mechanosensing by the integrin-actin axis has long been appreciated, a growing body of literature now demonstrates that cadherins also transduce and respond to mechanical forces. Mounting evid...

journal_title：Journal of cell science

authors： Mui KL,Chen CS,Assoian RK

更新日期：2016-03-15 00:00:00

abstract：:Intermediate filament protein keratin 8 (K8) binds to heat shock protein 70 (Hsp70) and p38 MAPK, and is phosphorylated at Ser74 by p38α (MAPK14, hereafter p38). However, a p38 binding site on K8 and the molecular mechanism of K8-p38 interaction related to Hsp70 are unknown. Here, we identify a p38 docking site on K8 ...

journal_title：Journal of cell science

authors： Lee SY,Kim S,Lim Y,Yoon HN,Ku NO

更新日期：2019-09-26 00:00:00

abstract：:The path that RNA takes through nuclear pores was mapped using two high-resolution techniques. Unexpectedly, no RNA in HL60 cells was detected by immunogold labelling in the central axis of the pore complex on its way to the transporter at the nuclear membrane; instead, it was distributed around the sides, apparently ...

journal_title：Journal of cell science

authors： Iborra FJ,Jackson DA,Cook PR

更新日期：2000-01-01 00:00:00

abstract：:Casein kinase 1 (CK1) plays central roles in various signal transduction pathways and performs many cellular activities. For many years CK1 was thought to act independently of modulatory subunits and in a constitutive manner. Recently, DEAD box RNA helicases, in particular DEAD box RNA helicase 3 X-linked (DDX3X), wer...

journal_title：Journal of cell science

authors： Dolde C,Bischof J,Grüter S,Montada A,Halekotte J,Peifer C,Kalbacher H,Baumann U,Knippschild U,Suter B

更新日期：2018-01-04 00:00:00

abstract：:The p53 tumor suppressor protein is a key regulator of cellular functions including responses to numerous stress signals, and triggers apoptosis in many cell types, including neurons. The major mechanisms known to regulate p53 stabilization and activation include phosphorylation and ubiquitin ligase-mediated proteasom...

journal_title：Journal of cell science

authors： Lee JH,Kim HS,Lee SJ,Kim KT

更新日期：2007-07-01 00:00:00

abstract：:Axonemal precursor tubulin is the major protein component of the detergent-soluble membrane/matrix fraction of sea urchin embryonic cilia. Its unusual abundance may reflect the rapid turnover of these cilia, a process that is further documented here. However, whether during induced regeneration or normal turnover and ...

journal_title：Journal of cell science

authors： Stephens RE

更新日期：1994-02-01 00:00:00

abstract：:A synthetic gene encoding a 'consensus' calmodulin (synCaM) was able to substitute for the Saccharomyces cerevisiae calmodulin gene (CMDI), even though synCaM is only 60% identical in primary amino acid sequence to yeast Cmd1. Twelve different synCaM mutants were also expressed in yeast. Seven of the 12 mutant synCaMs...

journal_title：Journal of cell science

authors： Harris E,Watterson DM,Thorner J

更新日期：1994-11-01 00:00:00

abstract：:To understand the molecular mechanisms underlying sorting of apical and basolateral membrane components in human intestinal epithelial cells, we have cloned the human homolog of rat syntaxin 3 and looked for its subcellular localization. Endogenous human syntaxin 3 was found to be localized at the apical membrane of c...

journal_title：Journal of cell science

authors： Delgrossi MH,Breuza L,Mirre C,Chavrier P,Le Bivic A

更新日期：1997-09-01 00:00:00

abstract：:The neuromuscular junction (NMJ) is the largest, most-complex synapse in the human body. Motor neuron (MN) diseases, such as amyotrophic lateral sclerosis (ALS), specifically target MNs and the NMJs. However, little is known about the reasons for MN-selective neuronal and synaptic vulnerability in MN diseases. Here, u...

journal_title：Journal of cell science

authors： Altman T,Geller D,Kleeblatt E,Gradus-Perry T,Perlson E

更新日期：2019-12-02 00:00:00

abstract：:Major histocompatibility complex class I (MHC-I) molecules signal infection or transformation by engaging receptors on T lymphocytes. The spatial organization of MHC-I on the plasma membranes is important for this engagement. We and others have shown that MHC-I molecules, like other membrane proteins, are not uniforml...

journal_title：Journal of cell science

authors： Blumenthal D,Edidin M,Gheber LA

更新日期：2016-09-01 00:00:00

abstract：:When expressed in pituitary AtT-20 cells, parotid proline-rich proteins enter the regulated pathway. Because the short N-terminal domain of a basic proline-rich protein is necessary for efficient export from the ER, it has not been possible to evaluate the role of this polypeptide segment as a sorting signal for regul...

journal_title：Journal of cell science

authors： Stahl LE,Wright RL,Castle JD,Castle AM

更新日期：1996-06-01 00:00:00

abstract：:Neutrophil leucocytes circulate in plasma, which contains broad-spectrum antiproteases such as alpha-2-macroglobulin and alpha-1-antitrypsin, and move into environments in which antiproteases are present. Plasma antiproteases significantly reduce the adhesiveness of leucocytes in both static and dynamic adhesion assay...

journal_title：Journal of cell science

authors： Forrester JV,Lackie JM,Brown AF

更新日期：1983-01-01 00:00:00

abstract：:To understand the mechanism involved in the apical sorting of glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) we fused to the C-terminus of GFP the GPI-anchor-attachment signal of the folate receptor (FR) or of the prion protein (PrP), two native GPI-anchored proteins that are sorted apically or basolat...

journal_title：Journal of cell science

authors： Paladino S,Lebreton S,Tivodar S,Campana V,Tempre R,Zurzolo C

更新日期：2008-12-15 00:00:00

abstract：:Mob1 is a component of both the mitotic exit network and Hippo pathway, being required for cytokinesis, control of cell proliferation and apoptosis. Cell division accuracy is crucial in maintaining cell ploidy and genomic stability and relies on the correct establishment of the cell division axis, which is under the c...

journal_title：Journal of cell science

authors： Tavares A,Gonçalves J,Florindo C,Tavares AA,Soares H

更新日期：2012-01-15 00:00:00

abstract：:An important aspect of wound healing is the recruitment of neutrophils to the site of infection or tissue injury. Lumican, an extracellular matrix component belonging to the small leucine rich proteoglycan (SLRP) family, is one of the major keratan sulfate proteoglycans (KSPGs) within the corneal stroma. Increasing ev...

journal_title：Journal of cell science

authors： Hayashi Y,Call MK,Chikama T,Liu H,Carlson EC,Sun Y,Pearlman E,Funderburgh JL,Babcock G,Liu CY,Ohashi Y,Kao WW

更新日期：2010-09-01 00:00:00

abstract：:The current model for hemoglobin ingestion and transport by intraerythrocytic Plasmodium falciparum malaria parasites shares similarities with endocytosis. However, the model is largely hypothetical, and the mechanisms responsible for the ingestion and transport of host cell hemoglobin to the lysosome-like food vacuol...

journal_title：Journal of cell science

authors： Lazarus MD,Schneider TG,Taraschi TF

更新日期：2008-06-01 00:00:00

abstract：:Faithful genome transmission requires a network of pathways coordinating DNA replication to DNA repair and recombination. Here, we used molecular combing to measure the impact of homologous recombination (HR) on the velocity of DNA replication forks. We used three hamster cell lines defective in HR either by overexpre...

journal_title：Journal of cell science

authors： Daboussi F,Courbet S,Benhamou S,Kannouche P,Zdzienicka MZ,Debatisse M,Lopez BS

更新日期：2008-01-15 00:00:00

abstract：:Removal of interleukin-3 from murine IC.DP pre-mast cells results in irreversible commitment to apoptosis within 18 hours. To identify early events necessary for the engagement of apoptosis we examined the regulation of intracellular pH (pH(i)). IC.DP cells acidified 2 hours after removal of interleukin-3 (before disc...

journal_title：Journal of cell science

authors： Chen Q,Benson RS,Whetton AD,Brant SR,Donowitz M,Montrose MH,Dive C,Watson AJ

更新日期：1997-02-01 00:00:00

abstract：:In skeletal muscle, the triad is a structure comprising a transverse (T)-tubule and sarcoplasmic reticulum (SR) cisternae. Triads constitute the basis of excitation-contraction coupling as the cradle of the Ca2+ release complex. We have shown previously that triadin, a member of this complex, has shaping properties on...

journal_title：Journal of cell science

authors： Osseni A,Sébastien M,Sarrault O,Baudet M,Couté Y,Fauré J,Fourest-Lieuvin A,Marty I

更新日期：2016-10-15 00:00:00