Abstract：
:Cell migration is essential for normal development and many pathological processes. Rho-family small GTPases play important roles in this event. In particular, Rac regulates lamellipodia formation at the leading edge during migration. The small GTPase RhoG activates Rac through its effector ELMO and the ELMO-binding protein Dock180, which functions as a Rac-specific guanine nucleotide exchange factor. Here we investigated the role of RhoG in cell migration. RNA interference-mediated knockdown of RhoG in HeLa cells reduced cell migration in Transwell and scratch-wound migration assays. In RhoG-knockdown cells, activation of Rac1 and formation of lamellipodia at the leading edge in response to wounding were attenuated. By contrast, expression of active RhoG promoted cell migration through ELMO and Dock180. However, the interaction of Dock180 with Crk was dispensable for the activation of Rac1 and promotion of cell migration by RhoG. Taken together, these results suggest that RhoG contributes to the regulation of Rac activity in migrating cells.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Katoh H,Hiramoto K,Negishi Mdoi
10.1242/jcs.02720keywords:
subject
Has Abstractpub_date
2006-01-01 00:00:00pages
56-65issue
Pt 1eissn
0021-9533issn
1477-9137pii
jcs.02720journal_volume
119pub_type
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