Abstract:
:We have identified a novel autoantibody specificity in scleroderma that we term anti-chromo. These antibodies recognize several chromosomal antigens with apparent molecular mass of between 23 and 25 kDa, as determined by immunoblots. Anti-chromo autoantibodies occur in 10-15% of sera from patients with anti-centromere antibodies (ACA). We used anti-chromo antibodies to screen a human expression library and obtained cDNA clones encoding a 25 kDa chromosomal autoantigen. DNA sequence analysis reveals this protein to be a human homologue of HP1, a heterochromatin protein of Drosophila melanogaster. We designate our cloned protein HP1Hs alpha. Epitope mapping experiments using both human and Drosophila HP1 reveal that anti-chromo antibodies target a region at the amino terminus of the protein. This region contains a conserved motif, the chromo domain (or HP1/Pc box), first recognized by comparison of Drosophila HP1 with the Polycomb gene product. Both proteins are thought to play a role in creating chromatin structures in which gene expression is suppressed. Anti-chromo thus defines a novel type of autoantibody that recognizes a conserved structural motif found on a number of chromosomal proteins.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Saunders WS,Chue C,Goebl M,Craig C,Clark RF,Powers JA,Eissenberg JC,Elgin SC,Rothfield NF,Earnshaw WCsubject
Has Abstractpub_date
1993-02-01 00:00:00pages
573-82eissn
0021-9533issn
1477-9137journal_volume
104 ( Pt 2)pub_type
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00462
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journal_title:Journal of cell science
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pub_type: 杂志文章
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journal_title:Journal of cell science
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doi:10.1242/jcs.02296
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journal_title:Journal of cell science
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doi:
更新日期:1996-05-01 00:00:00
abstract::Osteoclasts are giant multinucleated myeloid cells specialized for bone resorption, which is essential for the preservation of bone health throughout life. The activity of osteoclasts relies on the typical organization of osteoclast cytoskeleton components into a highly complex structure comprising actin, microtubules...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
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更新日期:2020-07-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1990-05-01 00:00:00
abstract::It is now becoming clear that additional transmembrane components can modify integrin-mediated adhesion. Syndecan-4 is a transmembrane heparan sulfate proteoglycan whose external glycosaminoglycan chains can bind extracellular matrix ligands and whose core protein cytoplasmic domain can signal during adhesion. Two pap...
journal_title:Journal of cell science
pub_type: 评论,杂志文章,评审
doi:
更新日期:1999-10-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2009-05-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.103028
更新日期:2012-07-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2013-05-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-06-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.089375
更新日期:2012-01-15 00:00:00
abstract::During vertebrate gastrulation, a well-orchestrated series of morphogenetic changes leads to the formation of the three germ layers: the ectoderm, mesoderm and endoderm. The analysis of gene expression patterns during gastrulation has been central to the identification of genes involved in germ layer formation. Howeve...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02928
更新日期:2006-05-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.138230
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.243303
更新日期:2020-08-11 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1982-08-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1985-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1985-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1999-12-01 00:00:00