Abstract:
:During anaphase-I in crane-fly spermatocytes, sister half-bivalents separate and move to opposite poles. When we irradiate a kinetochore spindle fibre with an ultraviolet microbeam, the associated half-bivalent temporarily stops moving and so does the partner half-bivalent with which it was paired during metaphase. To test whether a 'signal' is transmitted between partner half-bivalents we irradiated the spindle twice, once in the interzone (the region between separating partner half-bivalents) and once in a kinetochore fibre. For both irradiations we used light of wavelength 290 microns and a dose that, after irradiating a spindle fibre only, altered movement in 63% of irradiations (12/19); in 11 of the 12 cells both partner half-bivalents stopped moving after the irradiation. In control experiments we irradiated the interzone only: these irradiations generally did not stop chromosomal poleward motion but sometimes (14/29) caused poleward movement to each pole to be abruptly reduced to about half the velocity prior to irradiation. In double irradiation experiments we varied the order of the irradiations. In some double irradiation experiments we irradiated the interzonal region first and the spindle fibre second; in 75% (9/12) of the cells the half-bivalent associated with the irradiated fibre stopped moving while the partner half-bivalent moved normally, i.e. in 9/12 cells the interzonal irradiations uncoupled the movements of the partner half-bivalents. In other double irradiation experiments we irradiated the spindle fibre first and the interzone second: in 80% (4/5) of the cells the half-bivalents not associated with the irradiated spindle fibre resumed movement immediately after the irradiation while the other half-bivalent remained stopped. Interzonal irradiations therefore uncouple the poleward movements of sister half-bivalents and the uncoupling does not depend on the order of the irradiation. Our experiments suggest therefore that the irradiation of a spindle fibre causes negative ('stop') signals to be transmitted across the interzone and that irradiation of the interzone blocks the transmission of the stop signal.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Yin B,Forer Asubject
Has Abstractpub_date
1996-01-01 00:00:00pages
155-63eissn
0021-9533issn
1477-9137journal_volume
109 ( Pt 1)pub_type
杂志文章abstract::We analysed the phenotypic outcome of a Stra8-null mutation on male meiosis. Because the mutant spermatocytes (1) underwent premeiotic DNA replication, (2) displayed cytological features attesting initiation of recombination and of axial-element assembly, and (3) expressed Spo11 and numerous other meiotic genes, it wa...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.035071
更新日期:2008-10-01 00:00:00
abstract::Alpha-satellite (alphoid) DNA is necessary for de novo formation of human artificial chromosomes (HACs) in human cultured cells. To investigate the relationship among centromeric, transcriptionally permissive and non-permissive chromatin assemblies on de novo HAC formation, we constructed bacterial artificial chromoso...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02702
更新日期:2005-12-15 00:00:00
abstract::The COPII coat and the small GTPase Sar1 mediate protein export from the endoplasmic reticulum (ER) via specialized domains known as the ER exit sites. The peripheral ER protein Sec16 has been proposed to organize ER exit sites. However, it remains unclear how these molecules drive COPII coat polymerization. Here, we ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.203844
更新日期:2017-09-01 00:00:00
abstract::Kalirin, one of the few Rho guanine nucleotide exchange factors (GEFs) that contains spectrin-like repeats, plays a critical role in axon extension and maintenance of dendritic spines. PC12 cells were used to determine whether Cdk5, a critical participant in both processes, regulates the action of Kalirin. Expression ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.016089
更新日期:2008-08-01 00:00:00
abstract::Yeast cells were cultivated at different growth rates in a chemostat by alterations in the flow of the limiting nutrient glucose and in batch cultures where variations in growth rate were achieved by alterations in the composition of nutrients. It was observed that the stage in the cycle at which S-phase was completed...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1978-06-01 00:00:00
abstract::Mutation of the p53 gene is a common occurrence in human breast cancers but is by no means universal. However, even in tumours where the gene is not mutated altered levels of p53 protein are often detected. This is also observed in cell lines derived from human breast cancers. By transfecting such cell lines containin...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00
abstract::Microinjection of a bacterially expressed, TRITC labelled fragment of the centrosome-associated protein CP190 of Drosophila melanogaster, into syncytial Drosophila embryos, shows it to associate with the centrosomes during mitosis, and to relocate to chromatin during interphase. Indirect immunofluorescence staining of...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-11-01 00:00:00
abstract::Eggs of Urechis caupo are surrounded by a congruent to 0.9 micrometer thick egg envelope and, attached to that, a peripheral jelly layer about 3 micrometers thick. Before fertilization, the sperm undergoes the acrosome reaction and binds to the egg envelope. As part of a study of the induction of the acrosome reaction...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1985-03-01 00:00:00
abstract::The oligosaccharyltransferase (OST) complex catalyses the N-glycosylation of polypeptides entering the endoplasmic reticulum, a process essential for the productive folding and trafficking of many secretory and membrane proteins. In eukaryotes, the OST typically comprises a homologous catalytic STT3 subunit complexed ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.094599
更新日期:2012-01-01 00:00:00
abstract::Although the long-term effects of all-trans retinoic acid (RA) on neuronal growth and differentiation have been intensively studied, nothing is known about its effect on synaptic transmission. Here we show that RA rapidly and specifically enhances the spontaneous acetylcholine release at developing neuromuscular synap...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01153
更新日期:2004-06-15 00:00:00
abstract::In the presence of sialic acid donors Trypanosoma cruzi acquires up to 10(7) sialic acid residues on its surface, in a reaction catalyzed by its unique trans-sialidase. Most of these sialic acid residues are incorporated into mucin-like glycoproteins. To further understand the biological role of parasite sialylation, ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract::Anionic phospholipids PI(4,5)P2 and phosphatidylserine (PS) are enriched in the cytosolic leaflet of the plasma membrane where endocytic sites form. In this study, we investigated the roles of PI(4,5)P2 and PS in clathrin-mediated endocytosis (CME) site initiation and vesicle formation in Saccharomyces cerevisiae. Liv...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.115741
更新日期:2012-12-15 00:00:00
abstract::The development of DNA microarrays to study simultaneously the level of mRNA expressed from thousands of genes offers great promise to cell biologists. Microarrays can be used to gain detailed information about transcriptional changes involved in a specific pathway, potentially leading to the identification of novel c...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:
更新日期:2000-12-01 00:00:00
abstract::The binding of GFP-tagged tetracycline repressor (TetR) molecules to chromosomally integrated tetracycline operator (tetO) sequence repeats has been used as a system to study chromosome behaviour microscopically in vivo. We found that these integrated transgenes influence the architecture of yeast interphase nuclei, a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-03-15 00:00:00
abstract::The canonical Wnt/β-catenin signaling pathway regulates cell proliferation in development and adult tissue homeostasis. Dysregulated signaling contributes to human diseases, in particular cancer. Growing evidence suggests a role for clathrin and/or endocytosis in the regulation of this pathway, but conflicting results...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.244467
更新日期:2020-07-09 00:00:00
abstract::In hydra and in mammalian cells the monoclonal antibody V recognises an epitope which colocalises with cytoskeletal structures. Using this antibody for expression screening, a cDNA clone (955 bp) was isolated from hydra, which covers an open reading frame for a protein of 294 amino acids with a calculated molecular ma...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1991-12-01 00:00:00
abstract::Expression of a dominant negative fragment of the spectrin family member Syne-1 causes an accumulation of binucleate cells, suggesting a role for this protein in cytokinesis. An association of this fragment with the C-terminal tail domain of the kinesin II subunit KIF3B was identified by yeast two-hybrid and co-precip...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00892
更新日期:2004-02-01 00:00:00
abstract::CENP-C, one of the few known intrinsic proteins of the human centromere, is thought to play structural as well as regulatory roles crucial to proper chromosome segregation and mitotic progression. To further define the functions of CENP-C throughout the cell cycle we have used the yeast interaction trap to identify pr...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-07-30 00:00:00
abstract::The Dlg tumour suppressor protein is intimately involved in the control of cell contact and polarity. Previous studies have shown that hDlg is a target for a number of viral transforming proteins. In particular, the high risk human papillomavirus (HPV) E6 proteins target hDlg for proteasome-mediated degradation, an ac...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-12-01 00:00:00
abstract::MicroRNAs (miRNAs) play important roles in the development of skeletal muscle. In our previous study, expression of miR-195 and miR-497 were shown to be upregulated during muscle development in pigs. In this study, we investigated the roles of these two miRNAs in myogenesis and analyzed their transcriptional regulatio...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.174235
更新日期:2016-01-01 00:00:00
abstract::A universal feature of mitosis is that all chromosomes become aligned at the spindle equator--the halfway point between the two spindle poles--prior to anaphase onset. This migratory event is called congression, and is powered by centromere-bound protein machines called kinetochores. This Commentary aims to document r...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.169367
更新日期:2015-09-15 00:00:00
abstract::Myr 3, a member of the myosin-I family from rat, is shown in this study to be localized at adherens-type intercellular junctions in epithelial and nonepithelial tissues. Formation of intercellular junctions and the accompanying recruitment of myr 3 to these junctions involves signaling by the Rho subfamily of small GT...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract::Sorting nexins are a large family of phox-homology-domain-containing proteins that have been implicated in the control of endosomal sorting. Sorting nexin-1 is a component of the mammalian retromer complex that regulates retrieval of the cation-independent mannose 6-phosphate receptor from endosomes to the trans-Golgi...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02568
更新日期:2005-10-01 00:00:00
abstract::Heat shock transcription factor 1 (HSF1) regulates the expression of a wide array of genes, controls the expression of heat shock proteins (HSPs) as well as cell growth. Although acute depletion of HSF1 induces cellular senescence, the underlying mechanisms are poorly understood. Here, we report that HSF1 depletion-in...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.210724
更新日期:2018-05-08 00:00:00
abstract::Tumour necrosis factor receptors (TNF-Rs) and their ligands, tumour necrosis factors, are highly conserved proteins described in all metazoan phyla. They function as inducers of extrinsic apoptotic signalling and facilitate inflammation, differentiation and cell survival. TNF-Rs use distinct adaptor molecules to activ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.255422
更新日期:2021-01-25 00:00:00
abstract::Talin binds to β-integrin tails to activate integrins, regulating cell migration, invasion and metastasis. There are two talin genes, TLN1 and TLN2, encoding talin1 and talin2, respectively. Talin1 regulates focal adhesion dynamics, cell migration and invasion, whereas the biological function of talin2 is not clear an...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.185959
更新日期:2016-10-01 00:00:00
abstract::Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) triggers apoptosis selectively in tumor cells through interaction with TRAIL-R1/DR4 or TRAIL-R2/DR5 and this process is considered a promising avenue for cancer treatment. TRAIL resistance, however, is frequently encountered and hampers anti-cancer ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.109587
更新日期:2012-10-01 00:00:00
abstract::During the first meiotic prophase, alignment and synapsis of the homologous chromosomes are mediated by the synaptonemal complex. Incorrect assembly of this complex results in cell death, impaired meiotic recombination and formation of aneuploid germ cells. We have identified a novel mouse meiosis-specific protein, TE...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03182
更新日期:2006-10-01 00:00:00
abstract::Morphological, enzymic and antigenic data are presented regarding a human bone-marrow stromal cell line maintained for 10 months and subcultured weekly. The main characteristics are a fibroblastoid morphology, diffuse growth in collagen gels, no colony formation in soft gel media, contact inhibition of growth and conv...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1981-08-01 00:00:00
abstract::The mitotic spindle has long been recognized to play an essential role in determining the position of the cleavage furrow during cell division, however little is known about the mechanisms involved in this process. One attractive hypothesis is that signals from the spindle may function to induce reorganization of cort...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00