Abstract：
:Yeast cells were cultivated at different growth rates in a chemostat by alterations in the flow of the limiting nutrient glucose and in batch cultures where variations in growth rate were achieved by alterations in the composition of nutrients. It was observed that the stage in the cycle at which S-phase was completed varied with growth rate. The faster the growth rate, the earlier the stage in the cycle in which completion of S-phase occurred. When stage in the cycle is converted into time before division it was observed that the time from completion of S-phase to cell division varied only slightly with growth rate except at extremely slow growth rates. Expansion of cell cycle transit time as the growth rate was slowed was achieved primarily by an expansion in time of the period from division to the completion of S-phase. In contrast, when cells were grown at different rates by alterations in the temperature of cultivation, completion of S-phase occurred at approximately the same stage in the cell cycle at all growth rates.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Jagadish MN,Carter BLsubject
Has Abstractpub_date
1978-06-01 00:00:00pages
71-8eissn
0021-9533issn
1477-9137journal_volume
31pub_type
杂志文章abstract：:Previous work from this laboratory has indicated that very low concentrations of vinblastine block HeLa cells at mitosis in the presence of a full complement of microtubules and without major disruption of spindle organization. In the present study we analyzed the structural organization of mitotic spindle microtubule...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-02-01 00:00:00
abstract：:We have previously reported that foetal and adult fibroblasts display distinctive migratory phenotypes when cultured on three-dimensional collagen gels. Both skin and tumour-derived fibroblasts from a significant proportion of patients with breast cancer were subsequently observed to display foetal-like migratory beha...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1988-07-01 00:00:00
abstract：:The neuromuscular junction (NMJ) is the largest, most-complex synapse in the human body. Motor neuron (MN) diseases, such as amyotrophic lateral sclerosis (ALS), specifically target MNs and the NMJs. However, little is known about the reasons for MN-selective neuronal and synaptic vulnerability in MN diseases. Here, u...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.234492
更新日期：2019-12-02 00:00:00
abstract：:We first identified Bves (blood vessel/epicardial substance) as a transmembrane protein that localized to the lateral compartment of the epithelial epicardium. Bves traffics to sites of cell-cell contact in cultured epicardial cells and promotes adhesion following transfection into non-adherent fibroblastic L-cells, r...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02588
更新日期：2005-10-15 00:00:00
abstract：:Retinoic acid (RA) plays key roles in cell differentiation and growth arrest by activating nuclear RA receptors (RARs) (α, β and γ), which are ligand-dependent transcription factors. RARs are also phosphorylated in response to RA. Here, we investigated the in vivo relevance of the phosphorylation of RARs during RA-ind...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.145979
更新日期：2014-05-01 00:00:00
abstract：:Certain embryonic tissue masses and cell aggregates behave like deformable solids during brief experimental manipulations but like viscous liquids in long-term organ cultures. To investigate these seemingly paradoxical physical properties, we have mechanically deformed cell aggregates derived from several embryonic ch...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1978-04-01 00:00:00
abstract：:In order to determine whether fibronexus morphogenesis is involved in the establishment of more normal cellular morphology and substrate adhesion patterns in Nil/HSV transformed fibroblasts induced by treatment with exogenous fibronectin (FN), this system was studied with electron microscopy (EM), immunocytochemistry,...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1982-08-01 00:00:00
abstract：:The extracellular matrix molecule tenascin-R (TN-R) and the proteoglycans of the lectican family show an overlapping distribution in the developing brain, have been implicated in similar cellular processes and form a complex network of interactions. Previously, we have demonstrated that TN-R induces microprocesses alo...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03094
更新日期：2006-08-15 00:00:00
abstract：:The ultrastructure of the flagellar apparatus of Chlamydomonas moewusii was examined in detail. Two rhizoplasts were found associated with the basal bodies of this biflagellate and were observed to extend to the central area of the cell. A segment of smooth endoplasmic reticulum ran parallel to each rhizoplast. These ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1979-10-01 00:00:00
abstract：:Developing oral organelles of dividing Tetrahymena corlissi appear to be positioned by mechanisms which assess distances as a proportion of the organism's overall dimensions. In some respects, the cortex of this protozoan obeys the 'French flag' rule formulated by Wolpert for describing regulation of spatial proportio...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1976-06-01 00:00:00
abstract：:During spermatogenesis, the first morphological indication of spermatogonia differentiation is incomplete cytokinesis, followed by the assembly of stable intercellular cytoplasmic communications. This distinctive feature of differentiating male germ cells has been highly conserved during evolution, suggesting that reg...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00163
更新日期：2002-12-15 00:00:00
abstract：:gamma-Tubulin exists in fission yeast as the product of an essential gene, encoding a 446 amino acid protein that is 77.3% identical to Aspergillus nidulans gamma-tubulin. The gene disruption caused cell lethality, displaying condensed, undivided chromosomes with aberrant spindle structures. Anti-gamma-tubulin stainin...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-08-01 00:00:00
abstract：:Microtubules--polymers of tubulin--perform essential functions, including regulation of cell shape, intracellular transport and cell motility. How microtubules are adapted to perform multiple diverse functions is not well understood. Post-translational modifications of tubulin subunits diversify the outer and luminal ...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.063727
更新日期：2010-10-15 00:00:00
abstract：:Previously, we demonstrated that proteolytic processing within the globular domain of the alpha3 subunit of laminin-5 (LN5) converts LN5 from a cell motility-inducing factor to a protein complex that can trigger the formation of hemidesmosomes, certain cell-matrix attachment sites found in epithelial cells. We have pr...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-08-01 00:00:00
abstract：:Interstitial stem cells in Hydra are rapidly proliferating multipotent stem cells which continuously give rise to precursors for nerve and nematocyte differentiation. Growth of the stem cell population is controlled by the cell cycle time of the stem cells and the self-renewal probability, Ps (the fraction of stem cel...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1979-08-01 00:00:00
abstract：:Removal of interleukin-3 from murine IC.DP pre-mast cells results in irreversible commitment to apoptosis within 18 hours. To identify early events necessary for the engagement of apoptosis we examined the regulation of intracellular pH (pH(i)). IC.DP cells acidified 2 hours after removal of interleukin-3 (before disc...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：:The sclerotome portion of the differentiating embryonic chick somite becomes infiltrated by neural crest cells prior to its dispersal towards the embryonic axis. This means that sclerotome cells explanted into culture for the purpose of examining their interactions in vitro are contaminated with a proportion of neural...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1988-05-01 00:00:00
abstract：:The mitotic spindle has long been recognized to play an essential role in determining the position of the cleavage furrow during cell division, however little is known about the mechanisms involved in this process. One attractive hypothesis is that signals from the spindle may function to induce reorganization of cort...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
abstract：:Insulin granule trafficking is a key step of glucose-stimulated insulin secretion from pancreatic beta cells. Using quantitative live cell imaging, we examined insulin granule movements within the reserve pool upon secretory stimulation in betaTC3 cells. For this study, we developed a custom image analysis program tha...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02684
更新日期：2005-12-15 00:00:00
abstract：:Non-muscle myosin II (MyoII) contractility is central to the regulation of numerous cellular processes, including migration. Rho is a well-characterized modulator of actomyosin contractility, but the function of other GTPases, such as Rac, in regulating contractility is currently not well understood. Here, we show tha...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.131060
更新日期：2013-12-15 00:00:00
abstract：:Endocytosis of chemokine receptors regulates signal transduction initiated by chemokines, but the molecular mechanisms underlying this process are not fully defined. In this work, we assessed the involvement of the motor protein nonmuscle myosin heavy chain IIA (MIIA) in the endocytosis of CXCR4 induced by SDF-1alpha ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03415
更新日期：2007-03-15 00:00:00
abstract：:The cell nucleus is highly organized. Many nuclear functions are localized in discrete domains, suggesting that compartmentalization is an important aspect of the regulation and coordination of nuclear functions. We investigated the subnuclear distribution of the glucocorticoid receptor, a hormone-dependent transcript...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：:Cardiac fibrosis is linked to fibroblast-to-myofibroblast phenoconversion and proliferation but the mechanisms underlying this are poorly understood. Ski is a negative regulator of TGF-β-Smad signaling in myofibroblasts, and might redirect the myofibroblast phenotype back to fibroblasts. Meox2 could alter TGF-β-mediat...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.126722
更新日期：2014-01-01 00:00:00
abstract：:PACSIN2, a membrane-sculpting BAR domain protein, localizes to caveolae. Here, we found that protein kinase C (PKC) phosphorylates PACSIN2 at serine 313, thereby decreasing its membrane binding and tubulation capacities. Concomitantly, phosphorylation decreased the time span for which caveolae could be tracked at the ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.167775
更新日期：2015-08-01 00:00:00
abstract：:Anionic phospholipids PI(4,5)P2 and phosphatidylserine (PS) are enriched in the cytosolic leaflet of the plasma membrane where endocytic sites form. In this study, we investigated the roles of PI(4,5)P2 and PS in clathrin-mediated endocytosis (CME) site initiation and vesicle formation in Saccharomyces cerevisiae. Liv...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.115741
更新日期：2012-12-15 00:00:00
abstract：:Insulin-like growth factor-1 (IGF-1) has been shown to induce skeletal muscle hypertrophy, to prevent the loss of muscle mass with ageing and to improve the muscle phenotype of dystrophic mice. We previously developed a model of IGF-1-induced hypertrophy of human myotubes, in which hypertrophy was not only characteriz...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03371
更新日期：2007-02-15 00:00:00
abstract：:Mitochondria play essential roles in cellular energy processes, including ATP production, control of reactive oxygen species (ROS) and apoptosis. While mitochondrial function is regulated by the dynamics of fusion and fission, mitochondrial homeostasis remains incompletely understood. Recent studies implicate dynamin-...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.204537
更新日期：2017-07-15 00:00:00
abstract：:Using a reconstituted complex of profilin and skeletal muscle actin as an antigen, we generated a monoclonal mouse antibody against actin, termed 2G2. As revealed by immunoblots of proteolytic actin fragments and by pepscan analysis, the antibody recognises a nonsequential epitope on actin which is located within thre...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：:Distinctive views of the tubulo-vesicular elements interposed between the endoplasmic reticulum (ER) and the Golgi apparatus were obtained in thin sections. The tubules that protrude from the transitional rough ER (tRER) are of dissimilar length. The numbers of tubules and of the nearby omega- and pear-shaped profiles...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：:Nuclear sap, on fixation in glutaraldehyde, forms a fibrous network that resembles chromatin in its dimensions and staining properties. This artifactual network is easily confused with true chromatin fibres in sections of nuclei. With formaldehyde a homogeneous array of beads is produced. These are approximately 10 nm...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1977-08-01 00:00:00
