A three-dimensional reconstruction study of the rough ER-Golgi interface in serial thin sections of the pancreatic acinar cell of the rat.

abstract：:The neuroprotective role of carbon monoxide (CO) has been studied in a cell-autonomous mode. Herein, a new concept is disclosed - CO affects astrocyte-neuron communication in a paracrine manner to promote neuroprotection. Neuronal survival was assessed when co-cultured with astrocytes that had been pre-treated or not ...

journal_title：Journal of cell science

authors： Queiroga CS,Alves RM,Conde SV,Alves PM,Vieira HL

更新日期：2016-08-15 00:00:00

abstract：:Endothelial integrity relies on a mechanical crosstalk between intercellular and cell-matrix interactions. This crosstalk is compromised in hemorrhagic vascular lesions of patients carrying loss-of-function mutations in cerebral cavernous malformation (CCM) genes. RhoA/ROCK-dependent cytoskeletal remodeling is central...

journal_title：Journal of cell science

authors： Lisowska J,Rödel CJ,Manet S,Miroshnikova YA,Boyault C,Planus E,De Mets R,Lee HH,Destaing O,Mertani H,Boulday G,Tournier-Lasserve E,Balland M,Abdelilah-Seyfried S,Albiges-Rizo C,Faurobert E

更新日期：2018-08-13 00:00:00

abstract：:The scaffold attachment factor SAFB1 and its recently discovered homologue SAFB2 might provide an important link between pre-mRNA splicing, intracellular signalling and transcription. Using novel mono-specific antisera, we found endogenous SAFB2 protein has a different spatial distribution from SAFB1 within the nucleu...

journal_title：Journal of cell science

authors： Sergeant KA,Bourgeois CF,Dalgliesh C,Venables JP,Stevenin J,Elliott DJ

更新日期：2007-01-15 00:00:00

abstract：:Pregnancy-associated plasma protein A2 (PAPP-A2, also known as pappalysin-2) is a large metalloproteinase that is known to be required for normal postnatal growth and bone development in mice. We here report the detection of zebrafish papp-a2 mRNA in the chordamesoderm, notochord and lower jaw of zebrafish (Danio reri...

journal_title：Journal of cell science

authors： Kjaer-Sorensen K,Engholm DH,Jepsen MR,Morch MG,Weyer K,Hefting LL,Skov LL,Laursen LS,Oxvig C

更新日期：2014-12-01 00:00:00

abstract：:The microtubule-associated protein tau interacts with the SH3 domain of non-receptor Src family protein tyrosine kinases. A potential consequence of the SH3 interaction is the upregulation of tyrosine kinase activity. Here we investigated the activation of Src or Fyn by tau, both in vitro and in vivo. Tau increased th...

journal_title：Journal of cell science

authors： Sharma VM,Litersky JM,Bhaskar K,Lee G

更新日期：2007-03-01 00:00:00

abstract：:We have isolated a fourth myosin gene (MYO4) in yeast (Saccharomyces cerevisiae). MYO4 encodes a approximately 170 kDa (1471 amino acid) class V myosin, using the classification devised by Cheney et al. (1993a; Cell Motil. Cytoskel. 24, 215-223); the motor domain is followed by a neck region containing six putative ca...

journal_title：Journal of cell science

authors： Haarer BK,Petzold A,Lillie SH,Brown SS

更新日期：1994-04-01 00:00:00

abstract：:Cells that have been pre-exposed to thermal stress can acquire a transient resistance against the killing effect of a subsequent thermal stress. The cause for this phenomenon, called thermotolerance, seems to be an enhanced resistance of proteins against thermal denaturation and aggregation. This resistance can be exp...

journal_title：Journal of cell science

authors： Kampinga HH

更新日期：1993-01-01 00:00:00

abstract：:In the eukaryotic cell, a protein quality control process termed endoplasmic reticulum-associated degradation (ERAD) rids the ER of aberrant proteins and unassembled components of protein complexes that fail to reach a transport-competent state. To identify novel genes required for ERAD, we devised a rapid immunoassay...

journal_title：Journal of cell science

authors： Palmer EA,Kruse KB,Fewell SW,Buchanan SM,Brodsky JL,McCracken AA

更新日期：2003-06-01 00:00:00

abstract：:Vertebrate oocytes proceed through the first and the second meiotic division without an intervening S-phase to become haploid. Although DNA replication does not take place, unfertilized oocytes acquire the competence to replicate DNA one hour after the first meiotic division by accumulating an essential factor of the ...

journal_title：Journal of cell science

authors： Daldello EM,Le T,Poulhe R,Jessus C,Haccard O,Dupré A

更新日期：2015-07-15 00:00:00

abstract：:Members of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family of proteolytic enzymes are implicated in a variety of physiological processes, such as collagen maturation, organogenesis, angiogenesis, reproduction and inflammation. Moreover, deficiency or overexpression of certain ADAMTS ...

journal_title：Journal of cell science

authors： Llamazares M,Obaya AJ,Moncada-Pazos A,Heljasvaara R,Espada J,López-Otín C,Cal S

更新日期：2007-10-15 00:00:00

abstract：:Plasmid DNA or artificial mRNA injected intramuscularly into skeletal muscle via a 27 g needle expressed transgenes at relatively efficient levels in skeletal myofibers and cardiac cells. In the present study, several approaches were used to determine the mechanism of cellular uptake. After exposure of naked plasmid D...

journal_title：Journal of cell science

authors： Wolff JA,Dowty ME,Jiao S,Repetto G,Berg RK,Ludtke JJ,Williams P,Slautterback DB

更新日期：1992-12-01 00:00:00

abstract：:The formation of cytoplasmic stress granules and the innate immune response are two distinct cellular stress responses. Our study investigated the involvement of four innate immune proteins - retinoic-acid-inducible gene I (RIG-I, also known as DDX58), melanoma differentiation-associated gene 5 (MDA5, also known as IF...

journal_title：Journal of cell science

authors： Zhang P,Li Y,Xia J,He J,Pu J,Xie J,Wu S,Feng L,Huang X,Zhang P

更新日期：2014-06-01 00:00:00

abstract：:Mutations in a gene (Klp38B) encoding a novel kinesin-like protein in Drosophila melanogaster lead to the formation of polyploid cells in the larval central nervous system and in the follicle cells of adult egg chambers. Some homozygous mutants survive to adulthood and also exhibit morphological defects indicative of ...

journal_title：Journal of cell science

authors： Ohkura H,Török T,Tick G,Hoheisel J,Kiss I,Glover DM

更新日期：1997-04-01 00:00:00

abstract：:Annexin 1 is a Ca(2+)-regulated membrane binding protein and a major substrate of the epidermal growth factor receptor kinase. Because of its properties and intracellular distribution, the protein has been implicated in endocytic trafficking of the receptor, in particular in receptor sorting occurring in multivesicula...

journal_title：Journal of cell science

authors： Rescher U,Zobiack N,Gerke V

更新日期：2000-11-01 00:00:00

abstract：:Notch signalling is used throughout the animal kingdom to spatially and temporally regulate cell fate, proliferation and differentiation. Its importance is reflected in the dramatic effects produced on both development and health by small variations in the strength of the Notch signal. The Down-syndrome-associated kin...

journal_title：Journal of cell science

authors： Fernandez-Martinez J,Vela EM,Tora-Ponsioen M,Ocaña OH,Nieto MA,Galceran J

更新日期：2009-05-15 00:00:00

abstract：:MicroRNAs (miRNAs) play important roles in the development of skeletal muscle. In our previous study, expression of miR-195 and miR-497 were shown to be upregulated during muscle development in pigs. In this study, we investigated the roles of these two miRNAs in myogenesis and analyzed their transcriptional regulatio...

journal_title：Journal of cell science

authors： Wei W,Zhang WY,Bai JB,Zhang HX,Zhao YY,Li XY,Zhao SH

更新日期：2016-01-01 00:00:00

abstract：:The human Ras superfamily of small GTPases controls essential cellular processes such as gene expression and cell proliferation. As their deregulation is widely associated with human cancer, small GTPases and their regulatory proteins have become increasingly attractive for the development of novel therapeutics. Class...

journal_title：Journal of cell science

authors： Koraïchi F,Gence R,Bouchenot C,Grosjean S,Lajoie-Mazenc I,Favre G,Cabantous S

更新日期：2018-01-08 00:00:00

abstract：:The neuromuscular junction (NMJ) is the largest, most-complex synapse in the human body. Motor neuron (MN) diseases, such as amyotrophic lateral sclerosis (ALS), specifically target MNs and the NMJs. However, little is known about the reasons for MN-selective neuronal and synaptic vulnerability in MN diseases. Here, u...

journal_title：Journal of cell science

authors： Altman T,Geller D,Kleeblatt E,Gradus-Perry T,Perlson E

更新日期：2019-12-02 00:00:00

abstract：:The biosynthetic machinery in the melanotrope cells of the Xenopus intermediate pituitary is primarily dedicated to the generation of proopiomelanocortin (POMC)-derived, melanophore-stimulating peptides. Transfer of the animal to a black background stimulates the production of these peptides and causes a dramatic incr...

journal_title：Journal of cell science

authors： Holthuis JC,Jansen EJ,van Riel MC,Martens GJ

更新日期：1995-10-01 00:00:00

abstract：:It is generally accepted that global translation varies during the cell cycle and is low during mitosis. However, addressing this issue is challenging because it involves cell synchronization, which evokes stress responses that, in turn, affect translation rates. Here, we have used two approaches to measure global tra...

journal_title：Journal of cell science

authors： Stonyte V,Boye E,Grallert B

更新日期：2018-09-03 00:00:00

abstract：:We have found that Ehrlich ascites tumour (EAT) cells, deprived of any carbon source, and suspended at a density of 2 X 10(5) cells/cm3, begin to die only after 12 h of starvation, though it is known that under these conditions they lose over 80% of their ATP within 30 min. Moreover, we have found that the viability o...

journal_title：Journal of cell science

authors： Zaporowska-Siwiak E,Michalik M,Kajstura J,Korohoda W

更新日期：1985-08-01 00:00:00

abstract：:Rubella virus (RV) envelope glycoproteins E1 and E2 are targeted to the Golgi as heterodimers. While E2 contains a transmembrane Golgi retention signal, E1 is arrested in a pre-Golgi compartment in the absence of E2, and appears to require heterodimerization in order to reach the Golgi. Various forms of E1 with deleti...

journal_title：Journal of cell science

authors： Bernasconi E,Fasel N,Wittek R

更新日期：1996-06-01 00:00:00

abstract：:Maturation of nociceptive neurons depends on changes in transcription factors, ion channels and neuropeptides. Mature nociceptors initiate pain in part by drastically reducing the activation threshold via intracellular sensitization signaling. Whether sensitization signaling also changes during development and aging r...

journal_title：Journal of cell science

authors： Isensee J,Schild C,Schwede F,Hucho T

更新日期：2017-07-01 00:00:00

abstract：:Membrane contact sites (MCSs), where the membranes of two organelles are closely apposed, are regions where small molecules such as lipids or calcium are exchanged between organelles. We have identified a conserved membrane-binding domain found exclusively in proteins at MCSs in Saccharomyces cerevisiae. The synaptota...

journal_title：Journal of cell science

authors： Toulmay A,Prinz WA

更新日期：2012-01-01 00:00:00

abstract：:The serine/threonine protein phosphatase-1 (PP1) complex is a key regulator of the cell cycle. However, the redundancy of PP1 isoforms and the lack of specific inhibitors have hampered studies on the global role of PP1 in cell cycle progression in vertebrates. Here, we show that the overexpression of nuclear inhibitor...

journal_title：Journal of cell science

authors： Winkler C,De Munter S,Van Dessel N,Lesage B,Heroes E,Boens S,Beullens M,Van Eynde A,Bollen M

更新日期：2015-12-15 00:00:00

abstract：:Cells synthesize ceramides in the endoplasmic reticulum (ER) as precursors for sphingolipids to form an impermeable plasma membrane. As ceramides are engaged in apoptotic pathways, cells would need to monitor their levels closely to avoid killing themselves during sphingolipid biosynthesis. How this is accomplished re...

journal_title：Journal of cell science

authors： Tafesse FG,Vacaru AM,Bosma EF,Hermansson M,Jain A,Hilderink A,Somerharju P,Holthuis JC

更新日期：2014-01-15 00:00:00

abstract：:Mutations in the maternal-effect gene abnormal chromatin (abc) in Drosophila melanogaster result in a variety of defects involving nuclear replication/division. Three recessive alleles of this gene, which maps near 51F on chromosome 2, all result in female sterility. They cause slower embryonic development that is usu...

journal_title：Journal of cell science

authors： Vessey KB,Ludwiczak RL,Briot AS,Underwood EM

更新日期：1991-02-01 00:00:00

abstract：:During angiogenesis, endothelial cells must coordinate matrix proteolysis with migration. Here, we tested whether the focal adhesion scaffold protein Hic-5 (also known as TGFB1I1) regulated endothelial sprouting in three dimensions. Hic-5 silencing reduced endothelial sprouting and lumen formation, and sprouting defec...

journal_title：Journal of cell science

authors： Dave JM,Abbey CA,Duran CL,Seo H,Johnson GA,Bayless KJ

更新日期：2016-02-15 00:00:00

abstract：:During muscle development myosin molecules form symmetrical thick filaments, which integrate with the thin filaments to produce the regular sarcomeric lattice. In Drosophila indirect flight muscles (IFMs) the details of this process can be studied using genetic approaches. The weeP26 transgenic line has a GFP-encoding...

journal_title：Journal of cell science

authors： Orfanos Z,Sparrow JC

更新日期：2013-01-01 00:00:00

abstract：:Centaurin-alpha(1) is a phosphatidylinositol 3,4,5-trisphosphate binding protein as well as a GTPase activating protein (GAP) for the ADP-ribosylation factor (ARF) family of small GTPases. To further understand its cellular function, we screened a rat brain cDNA library using centaurin-alpha(1) as bait to identify cen...

journal_title：Journal of cell science

authors： Venkateswarlu K,Hanada T,Chishti AH

更新日期：2005-06-01 00:00:00