Phosphorylation of PACSIN2 by protein kinase C triggers the removal of caveolae from the plasma membrane.

Abstract:

:PACSIN2, a membrane-sculpting BAR domain protein, localizes to caveolae. Here, we found that protein kinase C (PKC) phosphorylates PACSIN2 at serine 313, thereby decreasing its membrane binding and tubulation capacities. Concomitantly, phosphorylation decreased the time span for which caveolae could be tracked at the plasma membrane (the 'tracking duration'). Analyses of the phospho-mimetic S313E mutant suggested that PACSIN2 phosphorylation was sufficient to reduce caveolar-tracking durations. Both hypotonic treatment and isotonic drug-induced PKC activation increased PACSIN2 phosphorylation at serine 313 and shortened caveolar-tracking durations. Caveolar-tracking durations were also reduced upon the expression of other membrane-binding-deficient PACSIN2 mutants or upon RNA interference (RNAi)-mediated PACSIN2 depletion, pointing to a role for PACSIN2 levels in modulating the lifetime of caveolae. Interestingly, the decrease in membrane-bound PACSIN2 was inversely correlated with the recruitment and activity of dynamin 2, a GTPase that mediates membrane scission. Furthermore, expression of EHD2, which stabilizes caveolae and binds to PACSIN2, restored the tracking durations of cells with reduced PACSIN2 levels. These findings suggest that the PACSIN2 phosphorylation decreases its membrane-binding activity, thereby decreasing its stabilizing effect on caveolae and triggering dynamin-mediated removal of caveolae.

journal_name

J Cell Sci

journal_title

Journal of cell science

authors

Senju Y,Rosenbaum E,Shah C,Hamada-Nakahara S,Itoh Y,Yamamoto K,Hanawa-Suetsugu K,Daumke O,Suetsugu S

doi

10.1242/jcs.167775

subject

Has Abstract

pub_date

2015-08-01 00:00:00

pages

2766-80

issue

15

eissn

0021-9533

issn

1477-9137

pii

jcs.167775

journal_volume

128

pub_type

杂志文章
  • gamma-Tubulin is present in acentriolar MTOCs during early mouse development.

    abstract::gamma-Tubulin, a recently discovered member of the tubulin superfamily, is a peri-centriolar component considered to be essential for microtubule nucleation. Mouse oocytes and early embryos lack centrioles until the blastocyst stage. Thus, early mouse embryos allowed us to study the location of gamma-tubulin in animal...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Gueth-Hallonet C,Antony C,Aghion J,Santa-Maria A,Lajoie-Mazenc I,Wright M,Maro B

    更新日期:1993-05-01 00:00:00

  • A mitotic kinesin-6, Pav-KLP, mediates interdependent cortical reorganization and spindle dynamics in Drosophila embryos.

    abstract::We investigated the role of Pav-KLP, a kinesin-6, in the coordination of spindle and cortical dynamics during mitosis in Drosophila embryos. In vitro, Pav-KLP behaves as a dimer. In vivo, it localizes to mitotic spindles and furrows. Inhibition of Pav-KLP causes defects in both spindle dynamics and furrow ingression, ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.064048

    authors: Sommi P,Ananthakrishnan R,Cheerambathur DK,Kwon M,Morales-Mulia S,Brust-Mascher I,Mogilner A

    更新日期:2010-06-01 00:00:00

  • Novel nuclear defects in KLP61F-deficient mutants in Drosophila are partially suppressed by loss of Ncd function.

    abstract::KLP61F in Drosophila and other BimC kinesins are essential for spindle bipolarity across species; loss of BimC function generates high frequencies of monopolar spindles. Concomitant loss of Kar3 kinesin function increases the frequency of bipolar spindles although the underlying mechanism is not known. Recent studies ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.01334

    authors: Wilson PG,Simmons R,Saighal S

    更新日期:2004-10-01 00:00:00

  • Suppression of apoptosis by v-ABL protein tyrosine kinase is associated with nuclear translocation and activation of protein kinase C in an interleukin-3-dependent haemopoietic cell line.

    abstract::We previously demonstrated that activation of v-ABL protein tyrosine kinase resulted in suppression of apoptosis following interleukin-3 removal using an interleukin-3-dependent haemopoietic cell line transfected with a temperature-sensitive mutant of the v-abl oncoprotein (IC.DP). Cellular signalling events associate...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Evans CA,Lord JM,Owen-Lynch PJ,Johnson G,Dive C,Whetton AD

    更新日期:1995-07-01 00:00:00

  • Ubiquitin-proteasome-dependent degradation of mammalian ER stearoyl-CoA desaturase.

    abstract::Mammalian Delta9 stearoyl-CoA desaturase 1 (SCD1) is a key enzyme in the biosynthesis of mono-unsaturated fatty acids in the endoplasmic reticulum (ER). It is a short-lived multispanning ER membrane protein, reported to be degraded by the ubiquitin-proteasome-independent pathway. We have examined SCD1 protein degradat...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.02951

    authors: Kato H,Sakaki K,Mihara K

    更新日期:2006-06-01 00:00:00

  • Tumor spheroid-induced vesicle formation on endothelial cells is associated with procoagulant properties.

    abstract::Fibrin deposits in tumor beds are an intriguing phenomenon. It has been suggested that fibrin plays a role as a provisional matrix in which the tumor grows and induces development of a vascular network. On the other hand fibrin possibly protects the tumor nodule from host defense mechanisms. We therefore investigate w...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Lichtenbeld HH,Muller AD,van Dam-Mieras MC,Blijham GH

    更新日期:1993-10-01 00:00:00

  • Agonistic monoclonal antibodies against the Met receptor dissect the biological responses to HGF.

    abstract::Hepatocyte growth factor, also known as scatter factor, is a pleiotropic cytokine, which stimulates cell motility, invasion, proliferation, survival and morphogenesis, and induces the expression of specific genes by activating its receptor tyrosine kinase. In this work we have isolated, characterized and used as agoni...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Prat M,Crepaldi T,Pennacchietti S,Bussolino F,Comoglio PM

    更新日期:1998-01-01 00:00:00

  • nimO, an Aspergillus gene related to budding yeast Dbf4, is required for DNA synthesis and mitotic checkpoint control.

    abstract::The nimO predicted protein of Aspergillus nidulans is related structurally and functionally to Dbf4p, the regulatory subunit of Cdc7p kinase in budding yeast. nimOp and Dbf4p are most similar in their C-termini, which contain a PEST motif and a novel, short-looped Cys2-His2 zinc finger-like motif. DNA labelling and re...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: James SW,Bullock KA,Gygax SE,Kraynack BA,Matura RA,MacLeod JA,McNeal KK,Prasauckas KA,Scacheri PC,Shenefiel HL,Tobin HM,Wade SD

    更新日期:1999-05-01 00:00:00

  • CLASP localizes in two discrete patterns on cortical microtubules and is required for cell morphogenesis and cell division in Arabidopsis.

    abstract::In animals and yeast, CLASP proteins are microtubule plus-end tracking proteins (+TIPS) involved in the regulation of microtubule plus-end dynamics and stabilization. Here we show that mutations in the Arabidopsis CLASP homolog result in various plant growth reductions, cell form defects and reduced mitotic activity. ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.024950

    authors: Kirik V,Herrmann U,Parupalli C,Sedbrook JC,Ehrhardt DW,Hülskamp M

    更新日期:2007-12-15 00:00:00

  • Conservation of the pro-apoptotic nuclease activity of endonuclease G in unicellular trypanosomatid parasites.

    abstract::Endonuclease G is a mitochondrial protein implicated in DNA fragmentation during apoptosis in cell types ranging from fungi to mammals. Features of programmed cell death have been reported in a number of single-celled organisms, including the human trypanosomatid parasites Leishmania and Trypanosoma. However, the prot...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.014050

    authors: Gannavaram S,Vedvyas C,Debrabant A

    更新日期:2008-01-01 00:00:00

  • End13p/Vps4p is required for efficient transport from early to late endosomes in Saccharomyces cerevisiae.

    abstract::end13-1 was isolated in a screen for endocytosis mutants and has been shown to have a post-internalisation defect in endocytic transport as well as a defect in vacuolar protein sorting (Vps(-) phenotype), leading to secretion of newly synthesised vacuolar proteins. Here we demonstrate that END13 is identical to VPS4, ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Zahn R,Stevenson BJ,Schröder-Köhne S,Zanolari B,Riezman H,Munn AL

    更新日期:2001-05-01 00:00:00

  • A quantitative analysis of connexin-specific permeability differences of gap junctions expressed in HeLa transfectants and Xenopus oocytes.

    abstract::Gap junctions provide direct intercellular communication by linking adjacent cells with aqueous pores permeable to molecules up to 1 kDa in molecular mass and 8-14 A in diameter. The identification of over a dozen connexins in the mammalian gap junction family has stimulated interest in the functional significance of ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Cao F,Eckert R,Elfgang C,Nitsche JM,Snyder SA,H-ulser DF,Willecke K,Nicholson BJ

    更新日期:1998-01-01 00:00:00

  • Sorting of actin isoforms in chicken auditory hair cells.

    abstract::Most nonmuscle cells of higher vertebrates contain two different actin isoforms, beta- and gamma-cytoplasmic actin. The beta-isoform is with few exceptions the predominant isoform in nonmuscle cells and tissues. Perturbation of the beta:gamma ratio has been shown to affect the organization of bundled actin filaments i...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Höfer D,Ness W,Drenckhahn D

    更新日期:1997-03-01 00:00:00

  • Trafficking of MHC molecules to the cell surface creates dynamic protein patches.

    abstract::Major histocompatibility complex class I (MHC-I) molecules signal infection or transformation by engaging receptors on T lymphocytes. The spatial organization of MHC-I on the plasma membranes is important for this engagement. We and others have shown that MHC-I molecules, like other membrane proteins, are not uniforml...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.187112

    authors: Blumenthal D,Edidin M,Gheber LA

    更新日期:2016-09-01 00:00:00

  • Growth-speed-correlated localization of exocyst and polarisome components in growth zones of Ashbya gossypii hyphal tips.

    abstract::We use the fungus Ashbya gossypii to investigate how its polar growth machinery is organized to achieve sustained hyphal growth. In slowly elongating hyphae exocyst, cell polarity and polarisome proteins permanently localize as cortical cap at hyphal tips, thus defining the zone of secretory vesicle fusion. In tenfold...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.033852

    authors: Köhli M,Galati V,Boudier K,Roberson RW,Philippsen P

    更新日期:2008-12-01 00:00:00

  • Src mediates the mechanical activation of myogenesis by activating TNFα-converting enzyme.

    abstract::Mechanical stimulation affects many biological aspects in living cells through mechanotransduction. In myogenic precursor cells (MPCs), mechanical stimulation activates p38 mitogen-activated protein kinase (MAPK), a key regulator of myogenesis, via activating TNFα-converting enzyme (TACE, also known as ADAM17), to rel...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.125328

    authors: Niu A,Wen Y,Liu H,Zhan M,Jin B,Li YP

    更新日期:2013-10-01 00:00:00

  • Concentration of pp125 focal adhesion kinase (FAK) at the myotendinous junction.

    abstract::Focal adhesion kinase is a recently characterized tyrosine kinase that is concentrated at focal contacts in cultured cells. It is thought to play an important role in the regulation of the integrin-based signal transduction mechanism involved in the assembly of this membrane specialization. In this study, we examined ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Baker LP,Daggett DF,Peng HB

    更新日期:1994-06-01 00:00:00

  • Rhizoplast and rootlet system of the flagellar apparatus of Chlamydomonas moewusii.

    abstract::The ultrastructure of the flagellar apparatus of Chlamydomonas moewusii was examined in detail. Two rhizoplasts were found associated with the basal bodies of this biflagellate and were observed to extend to the central area of the cell. A segment of smooth endoplasmic reticulum ran parallel to each rhizoplast. These ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Katz KR,McLean RJ

    更新日期:1979-10-01 00:00:00

  • Epigenetic assembly of centromeric chromatin at ectopic alpha-satellite sites on human chromosomes.

    abstract::To investigate the mechanism of chromatin assembly at human centromeres, we isolated cultured human cell lines in which a transfected alpha-satellite (alphoid) YAC was integrated ectopically into the terminal region of host chromosome 16, where it was stably maintained. Centromere activity of the alphoid YAC was suppr...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.00697

    authors: Nakano M,Okamoto Y,Ohzeki J,Masumoto H

    更新日期:2003-10-01 00:00:00

  • Isoform B of myosin II heavy chain mediates actomyosin contractility during TNFalpha-induced apoptosis.

    abstract::Cells that are treated long-term with TNFalpha or short-term with TGFalpha together with cycloheximide (CHX) undergo apoptosis. Cell shrinkage and detachment during apoptosis is dependent on actomyosin contractility. Myosin II heavy chain (MHCII) isoforms have shared and distinct functions. Here, we investigated wheth...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.022640

    authors: Solinet S,Vitale ML

    更新日期:2008-05-15 00:00:00

  • Characterization of a novel meiosis-specific protein within the central element of the synaptonemal complex.

    abstract::During the first meiotic prophase, alignment and synapsis of the homologous chromosomes are mediated by the synaptonemal complex. Incorrect assembly of this complex results in cell death, impaired meiotic recombination and formation of aneuploid germ cells. We have identified a novel mouse meiosis-specific protein, TE...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.03182

    authors: Hamer G,Gell K,Kouznetsova A,Novak I,Benavente R,Höög C

    更新日期:2006-10-01 00:00:00

  • The dynamic properties of intermediate filaments during organelle transport.

    abstract::Intermediate filament (IF) dynamics during organelle transport and their role in organelle movement were studied using Xenopus laevis melanophores. In these cells, pigment granules (melanosomes) move along microtubules and microfilaments, toward and away from the cell periphery in response to alpha-melanocyte stimulat...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.046789

    authors: Chang L,Barlan K,Chou YH,Grin B,Lakonishok M,Serpinskaya AS,Shumaker DK,Herrmann H,Gelfand VI,Goldman RD

    更新日期:2009-08-15 00:00:00

  • Very low cadmium concentrations stimulate DNA synthesis and cell growth.

    abstract::Uptake of cadmium into cultured cells and its effects on cell growth and DNA synthesis are measured over a range of Cd concentrations of seven orders of magnitude. Cd uptake is found to be proportional to the external Cd concentration and to incubation time over a very broad range of concentrations. At least 200 mmol ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: von Zglinicki T,Edwall C,Ostlund E,Lind B,Nordberg M,Ringertz NR,Wroblewski J

    更新日期:1992-12-01 00:00:00

  • Cytoplasmic dynein-2 at a glance.

    abstract::Cytoplasmic dynein-2 is a motor protein complex that drives the movement of cargoes along microtubules within cilia, facilitating the assembly of these organelles on the surface of nearly all mammalian cells. Dynein-2 is crucial for ciliary function, as evidenced by deleterious mutations in patients with skeletal abno...

    journal_title:Journal of cell science

    pub_type: 杂志文章,评审

    doi:10.1242/jcs.240614

    authors: Vuolo L,Stevenson NL,Mukhopadhyay AG,Roberts AJ,Stephens DJ

    更新日期:2020-03-30 00:00:00

  • Algal endosymbiosis in brown hydra: host/symbiont specificity.

    abstract::Host/symbiont specificity has been investigated in non-symbiotic and aposymbiotic brown and green hydra infected with various free-living and symbiotic species and strains of Chlorella and Chlorococcum. Morphology and ultrastructure of the symbioses obtained have been compared. Aposymbiotic Swiss Hydra viridis and Jap...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Rahat M,Reich V

    更新日期:1986-12-01 00:00:00

  • The Aspergillus nidulans bimE (blocked-in-mitosis) gene encodes multiple cell cycle functions involved in mitotic checkpoint control and mitosis.

    abstract::The bimE (blocked-in-mitosis) gene appears to function as a negative mitotic regulator because the recessive bimE7 mutation can override certain interphase-arresting treatments and mutations, causing abnormal induction of mitosis. We have further investigated the role of bimE in cell cycle checkpoint control by: (1) c...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: James SW,Mirabito PM,Scacheri PC,Morris NR

    更新日期:1995-11-01 00:00:00

  • Caenorhabditis elegans p97 controls germline-specific sex determination by controlling the TRA-1 level in a CUL-2-dependent manner.

    abstract::p97 (CDC-48 in Caenorhabditis elegans) is a ubiquitin-selective AAA (ATPases associated with diverse cellular activities) chaperone and its key function is to disassemble protein complexes. p97 functions in diverse cellular processes including endoplasmic reticulum (ER)-associated degradation, membrane fusion, and mei...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.052415

    authors: Sasagawa Y,Otani M,Higashitani N,Higashitani A,Sato K,Ogura T,Yamanaka K

    更新日期:2009-10-15 00:00:00

  • Inhibitors of the V0 subunit of the vacuolar H+-ATPase prevent segregation of lysosomal- and secretory-pathway proteins.

    abstract::The vacuolar H(+)-ATPase (V-ATPase) establishes pH gradients along secretory and endocytic pathways. Progressive acidification is essential for proteolytic processing of prohormones and aggregation of soluble content proteins. The V-ATPase V(0) subunit is thought to have a separate role in budding and fusion events. P...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.034298

    authors: Sobota JA,Bäck N,Eipper BA,Mains RE

    更新日期:2009-10-01 00:00:00

  • Rho-family GTPases: it's not only Rac and Rho (and I like it).

    abstract::The Rho-family proteins make up a major branch of the Ras superfamily of small GTPases. To date, 22 human genes encoding at least 25 proteins have been described. The best known 'classical' members are RhoA, Rac1 and Cdc42. Highly related isoforms of these three proteins have not been studied as intensively, in part b...

    journal_title:Journal of cell science

    pub_type: 杂志文章,评审

    doi:10.1242/jcs.01118

    authors: Wennerberg K,Der CJ

    更新日期:2004-03-15 00:00:00

  • Factors involved in the cell density-dependent regulation of nuclear/cytoplasmic distribution of the 11.5-kDa Zn(2+)-binding protein (parathymosin-alpha) in rat hepatocytes.

    abstract::Although the 11.5 kDa Zn(2+)-binding protein (ZnBP, parathymosin-alpha) possesses a functional bipartite nuclear localization signal it was found in most tissues in the cytoplasm. The cultivation of freshly isolated rat hepatocytes for 24 hours under standard conditions was associated with an almost complete transloca...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Trompeter HI,Schiermeyer A,Blankenburg G,Hennig E,Söling HD

    更新日期:1999-11-01 00:00:00