Abstract：
:gamma-Tubulin exists in fission yeast as the product of an essential gene, encoding a 446 amino acid protein that is 77.3% identical to Aspergillus nidulans gamma-tubulin. The gene disruption caused cell lethality, displaying condensed, undivided chromosomes with aberrant spindle structures. Anti-gamma-tubulin staining showed that gamma-tubulin is located, throughout the wild-type cell cycle, at the spindle pole bodies (SPBs), indicating that gamma-tubulin associates with interphase SPB in the absence of microtubules. In addition, anti-gamma-tubulin immunofluorescence staining revealed cytoplasmic, cell-equatorial putative MTOCs (microtubule organizing centers), which appear only during mitotic telophase and cytokinesis, and are located at the centers for the new cytoplasmic microtubule arrays of the two daughter cells. In the multiple-SPB mutant cut1-cdc11, anti-gamma-tubulin antibodies revealed many dots on the periphery of the nucleus. These results confirm that gamma-tubulin is an important member of the tubulin superfamily, suggest that it may be a universal component of MTOCs, and are consistent with a role for gamma-tubulin in controlling microtubule formation in vivo.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Horio T,Uzawa S,Jung MK,Oakley BR,Tanaka K,Yanagida Msubject
Has Abstractpub_date
1991-08-01 00:00:00pages
693-700eissn
0021-9533issn
1477-9137journal_volume
99 ( Pt 4)pub_type
杂志文章abstract：:Rat small hepatocytes have been shown to proliferate in culture and to form organoids with differentiated hepatocytes in vitro. To evaluate the degree of polarized transporter differentiation of rat small hepatocytes during 9 weeks of culturing, we studied the time-dependent expression and subcellular localization of ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01279
更新日期：2004-08-15 00:00:00
abstract：:Microtubule-associated protein 2 (MAP2) is an abundant neuron-specific protein that binds to microtubules through a domain near its carboxyl terminus that contains either three or four similar repeats of a 31 amino acid motif. When expressed in non-neuronal cells by transfection MAP2 stabilises microtubules and induce...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-11-01 00:00:00
abstract：:Cells undergo a variety of physiological processes, including division, migration and differentiation, under the influence of endogenous electrical cues, which are generated physiologically and pathologically in the extracellular and sometimes intracellular spaces. These signals are transduced to regulate cell behavio...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.023564
更新日期：2009-12-01 00:00:00
abstract：:The polarized functions of epithelia require an intact tight junction (TJ) to restrict paracellular movement and to separate membrane proteins into specific domains. TJs contain scaffolding, integral membrane and signaling proteins, but the mechanisms that regulate TJs and their assembly are not well defined. Galpha12...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.014878
更新日期：2008-03-15 00:00:00
abstract：:Endothelial cell-based angiogenesis requires activation of survival signals that generate resistance to external apoptotic stimuli, such as tumor necrosis factor-alpha (TNF-alpha), during pathobiologic settings. Mechanisms by which this is achieved are not fully defined. Here, we use a model in which the multifunction...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03361
更新日期：2007-02-01 00:00:00
abstract：:Endocytosis of chemokine receptors regulates signal transduction initiated by chemokines, but the molecular mechanisms underlying this process are not fully defined. In this work, we assessed the involvement of the motor protein nonmuscle myosin heavy chain IIA (MIIA) in the endocytosis of CXCR4 induced by SDF-1alpha ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03415
更新日期：2007-03-15 00:00:00
abstract：:The recently identified 63 kDa membrane protein, p63, is a resident protein of a membrane network interposed in between rough ER and Golgi apparatus. To characterize p63 at the molecular level a 2.91 kb cDNA encoding p63 has been isolated from a human placenta lambda gt10 cDNA library. Sequence analysis of tryptic pep...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-03-01 00:00:00
abstract：:Regulated, programmed cell death is crucial for all multicellular organisms. Cell death is essential in many processes, including tissue sculpting during embryogenesis, development of the immune system and destruction of damaged cells. The best-studied form of programmed cell death is apoptosis, a process that require...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.093575
更新日期：2014-05-15 00:00:00
abstract：:Although epithelial-mesenchymal (stromal) interactions are thought to play an important role in embryonic and postnatal development of the mammary gland, the underlying mechanisms are still poorly understood. To address this issue, we assessed the effect of fibroblast-derived diffusible factors on the growth and morph...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-02-01 00:00:00
abstract：:Cell chemotaxis, such as migration of fibroblasts towards growth factors during development and wound healing, requires precise spatial coordination of signalling events. Phosphoinositides and signalling enzymes involved in their generation and hydrolysis have been implicated in regulation of chemotaxis; however, the ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.110007
更新日期：2012-12-01 00:00:00
abstract：:Rapid endocytosis of lipoprotein receptors involves NPxY signals contained in their cytoplasmic tails. Several proteins, including ARH and Dab2, can bind these sequences, but their importance for endocytosis may vary in different cell types. The lipoprotein receptor megalin is expressed in the visceral endoderm (VE), ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02650
更新日期：2005-11-15 00:00:00
abstract：:A line of null mice has been produced which fails to express the transmembrane chondroitin sulfate proteoglycan NG2. Homozygous NG2 null mice do not exhibit gross phenotypic differences from wild-type mice, suggesting that detailed analyses are required to detect subtle alterations caused by the absence of NG2. Accord...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：:We have previously shown that Xenopus oocytes, eggs, and early embryos contain lamins LII and LIII, and that portions of each are associated with distinct egg vesicle populations. We now report that a lamin similar or identical to the B-type lamin LI is also present in oocyte nuclei and in egg extracts. We have quanti...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:The end1 mutant belongs to a group of four vacuolar protein sorting mutants (class C vps) that lack a morphologically distinguishable and functional vacuole. These mutants share several other phenotypes, such as the inability to grow at 37 degrees C or on nonfermentable carbon sources. We show that, as in the case of ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1990-11-01 00:00:00
abstract：:The mAb RK7, previously shown to recognize keratin 19, was also found to cross-react with a biologically unrelated 102 kDa protein, which becomes associated with the poles of the mitotic apparatus. This newly identified protein, called cytocentrin, is a stable cellular component, may be at least in part phosphorylated...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
abstract：:When Swiss 3T3 cells are treated with colcemid for 60 min (0.5-2 micrograms/ml), although the cytoplasmic network of microtubules is considerably reduced, a significant but variable number of microtubules remain. These residual microtubules appear to converge on a single, usually ciliated, organizing centre. In contra...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:An essential step in mammalian fertilisation is the sperm acrosome reaction (AR) - exocytosis of a single large vesicle (the acrosome) that surrounds the nucleus at the apical sperm head. The acrosomal and plasma membranes fuse, resulting in both the release of factors that might facilitate penetration of the zona pel...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.030379
更新日期：2008-07-01 00:00:00
abstract：:Eggs of Urechis caupo are surrounded by a congruent to 0.9 micrometer thick egg envelope and, attached to that, a peripheral jelly layer about 3 micrometers thick. Before fertilization, the sperm undergoes the acrosome reaction and binds to the egg envelope. As part of a study of the induction of the acrosome reaction...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1985-03-01 00:00:00
abstract：:The identification and characterization of scaffold and targeting proteins of the ERK/MAP kinase pathway is important to understand the function and intracellular organization of this pathway. The F-actin binding protein leukocyte-specific protein 1 (LSP1) binds to PKCbetaI and expression of B-LSP1, an LSP1 truncate c...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00955
更新日期：2004-04-15 00:00:00
abstract：:A high molecular weight tight junction-associated protein, ZO-1, has been demonstrated in liver (hepatocytes) and in both epithelium and endothelium. We carried out studies to examine the presence of the protein in vascular endothelial cell cultures and several other types of cultured cells, and the relationship betwe...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：:Membrane-bound transferrin-like protein (MTf), a glycosylphosphatidylinositol-anchored protein, is expressed at high levels in many tumors and in several fetal and adult tissues including cartilage and the intestine, as well as in the amyloid plaques of Alzheimer's disease, although its role remains unknown. MTf is on...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00393
更新日期：2003-05-15 00:00:00
abstract：:MicroRNAs are known to play regulatory roles in gene expression associated with cancer development. We analyzed levels of the microRNA miR-24 in patients with breast carcinoma and found that miR-24 was higher in breast carcinoma samples than in benign breast tissues. We generated constructs expressing miR-24 and studi...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.118299
更新日期：2013-03-15 00:00:00
abstract：:During mitosis, kinetochores need to attach to microtubules emanating from spindle poles. Several protein complexes have been shown to mediate the kinetochore-microtubule interaction. However, with the continually growing number of newly identified kinetochore proteins, it is unclear whether all major components of th...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.051912
更新日期：2009-07-15 00:00:00
abstract：:Mammalian homologs of the Drosophila TRP protein have been shown to form cation-permeable channels in the plasma membrane but very little is known about the mechanisms that control their cell surface localization. Recently it has been demonstrated that the last three C-terminal amino acids (TRL) of TRPC4 comprise a PD...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-09-01 00:00:00
abstract：:The tandemly organised ribosomal DNA (rDNA) repeats are transcribed by a dedicated RNA polymerase in a specialised nuclear compartment, the nucleolus. There appears to be an intimate link between the maintenance of nucleolar structure and the presence of heterochromatic chromatin domains. This is particularly evident ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-12-01 00:00:00
abstract：:The 16 kDa proteolipid (subunit c) of the eukaryotic vacuolar H(+)-ATPase (V-ATPase) is closely related to the ductin polypeptide that forms the connexon channel of gap junctions in the crustacean Nephrops norvegicus. Here we show that the major protein component of Manduca sexta gap junction preparations is a 16 kDa ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-07-01 00:00:00
abstract：:The integrin beta1 cytoplasmic domain plays a key role in a variety of integrin-mediated events including adhesion, migration and signaling. A number of studies suggest that phosphorylation may modify the functional state of the cytoplasmic domain, but these studies frequently only examine the effect of substituting a...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：:The process of angiogenesis requires endothelial cells (ECs) to undergo profound changes in shape and polarity. Although this must involve remodelling of the EC cytoskeleton, little is known about this process or the proteins that control it. We used a co-culture assay of angiogenesis to examine the cytoskeleton of EC...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.091066
更新日期：2012-03-15 00:00:00
abstract：:Host/symbiont specificity has been investigated in non-symbiotic and aposymbiotic brown and green hydra infected with various free-living and symbiotic species and strains of Chlorella and Chlorococcum. Morphology and ultrastructure of the symbioses obtained have been compared. Aposymbiotic Swiss Hydra viridis and Jap...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1986-12-01 00:00:00
abstract：:The microtubule-associated protein tau is a developmentally regulated family of neuronal phosphoproteins that promotes the assembly and stabilization of microtubules. The carboxy-terminal half of the protein contains three copies of an imperfectly repeated sequence; this region has been found to bind microtubules in v...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-06-01 00:00:00
