Characterization of the ZO-1 protein in endothelial and other cell lines.

abstract：:During pre-mRNA splicing, introns are removed and exons are ligated to form an mRNA. Exon choice is determined by different nuclear protein concentrations varying among tissues and cell types or by developmental stage. These can be altered by different cellular circumstances such as physiological stimuli, environmenta...

journal_title：Journal of cell science

authors： Shomron N,Alberstein M,Reznik M,Ast G

更新日期：2005-03-15 00:00:00

abstract：:Nedd1 is a new member of the gamma-tubulin ring complex (gammaTuRC) and targets the gammaTuRC to the centrosomes for microtubule nucleation and spindle assembly in mitosis. Although its role is known, its functional regulation mechanism remains unclear. Here we report that the function of Nedd1 is regulated by Cdk1 an...

journal_title：Journal of cell science

authors： Zhang X,Chen Q,Feng J,Hou J,Yang F,Liu J,Jiang Q,Zhang C

更新日期：2009-07-01 00:00:00

abstract：:The cross-striated muscle from the heart ventricle and the smooth penis retractor muscle of the freshwater snail Lymnaea stagnalis have been investigated by X-ray microanalysis to establish whether lanthanum can cross the plasma membrane, as has been reported by other investigators. Tissues were incubated in 1 mM ioni...

journal_title：Journal of cell science

authors： Swales LS,Gardner DR

更新日期：1985-04-01 00:00:00

abstract：:High-throughput neurotransmission at ribbon synapses of cochlear inner hair cells (IHCs) requires tight coupling of neurotransmitter release and balanced recycling of synaptic vesicles (SVs) as well as rapid restoration of release sites. Here, we examined the role of the adaptor protein AP180 (also known as SNAP91) fo...

journal_title：Journal of cell science

authors： Kroll J,Özçete ÖD,Jung S,Maritzen T,Milosevic I,Wichmann C,Moser T

更新日期：2020-01-22 00:00:00

abstract：:Sonic hedgehog (Shh) and its patched-smoothened receptor complex control a variety of functions in the developing central nervous system, such as neural cell proliferation and differentiation. Recently, Shh signaling components have been found to be expressed at the synaptic level in the postnatal brain, suggesting a ...

journal_title：Journal of cell science

authors： Delmotte Q,Hamze M,Medina I,Buhler E,Zhang J,Belgacem YH,Porcher C

更新日期：2020-10-23 00:00:00

abstract：:The Na(+)/H(+) exchanger NHE1 is involved in intracellular pH homeostasis and cell volume regulation and accumulates with actin in the lamellipodia of fibroblasts. In order to determine the role of NHE1 following epithelial transformation and the acquisition of motile and invasive properties, we studied NHE1 expressio...

journal_title：Journal of cell science

authors： Lagana A,Vadnais J,Le PU,Nguyen TN,Laprade R,Nabi IR,Noël J

更新日期：2000-10-01 00:00:00

abstract：:Rat small hepatocytes have been shown to proliferate in culture and to form organoids with differentiated hepatocytes in vitro. To evaluate the degree of polarized transporter differentiation of rat small hepatocytes during 9 weeks of culturing, we studied the time-dependent expression and subcellular localization of ...

journal_title：Journal of cell science

authors： Sidler Pfändler MA,Höchli M,Inderbitzin D,Meier PJ,Stieger B

更新日期：2004-08-15 00:00:00

abstract：:Stem cell regeneration is crucial for both cell turnover and tissue healing in multicellular organisms. In Arabidopsis roots, a reduced group of cells known as the quiescent center (QC) act as a cell reservoir for surrounding stem cells during both normal growth and in response to external damage. Although cells of th...

journal_title：Journal of cell science

authors： Lozano-Elena F,Planas-Riverola A,Vilarrasa-Blasi J,Schwab R,Caño-Delgado AI

更新日期：2018-01-29 00:00:00

abstract：:Human skin diseases have revealed fundamental mechanisms by which cytoskeletal proteins contribute to tissue architecture and function. In particular, the analysis of epidermal blistering disorders and the role of keratin gene mutations in these diseases has led to significant increases in our understanding of interme...

journal_title：Journal of cell science

authors： Kottke MD,Delva E,Kowalczyk AP

更新日期：2006-03-01 00:00:00

abstract：:Membrane contact sites (MCSs), where the membranes of two organelles are closely apposed, are regions where small molecules such as lipids or calcium are exchanged between organelles. We have identified a conserved membrane-binding domain found exclusively in proteins at MCSs in Saccharomyces cerevisiae. The synaptota...

journal_title：Journal of cell science

authors： Toulmay A,Prinz WA

更新日期：2012-01-01 00:00:00

abstract：:Neutrophil leucocytes circulate in plasma, which contains broad-spectrum antiproteases such as alpha-2-macroglobulin and alpha-1-antitrypsin, and move into environments in which antiproteases are present. Plasma antiproteases significantly reduce the adhesiveness of leucocytes in both static and dynamic adhesion assay...

journal_title：Journal of cell science

authors： Forrester JV,Lackie JM,Brown AF

更新日期：1983-01-01 00:00:00

abstract：:Prostaglandins E1, E2, and F2alpha (PGE1, PGE2, and PGF2alpha) were shown to inhibit the growth of mouse leukaemia lymphoblasts L5178Y in culture. The effects of PGE1 and PGE2 were greater than that of PGF2alpha. PGE1 and PGE2, at the concentration of 100 mug per ml showed significant inhibitory effects on the rates o...

journal_title：Journal of cell science

authors： Yang TJ,Dale JB,Machanoff R

更新日期：1976-01-01 00:00:00

abstract：:Phosphoinositides regulate myriad cellular processes, acting as potent signaling molecules in conserved signaling pathways and as organelle gatekeepers that recruit effector proteins to membranes. Phosphoinositide-generating enzymes have been studied extensively in yeast and cultured cells, yet their roles in animal d...

journal_title：Journal of cell science

authors： Tan J,Oh K,Burgess J,Hipfner DR,Brill JA

更新日期：2014-03-01 00:00:00

abstract：:ABCG2 belongs to the ATP-binding cassette superfamily of transmembrane proteins and is ubiquitously expressed in stem cells including those in the developing nervous system. The ability of ABCG2 to preferentially exclude DNA-intercalating dyes is regarded to be the basis for the enrichment of stem cells or progenitors...

journal_title：Journal of cell science

authors： Bhattacharya S,Das A,Mallya K,Ahmad I

更新日期：2007-08-01 00:00:00

abstract：:In order to replicate, most pathogens need to enter their target cells. Many viruses enter the host cell through an endocytic pathway and hijack endosomes for their journey towards sites of replication. For delivery of their genome to the host cell cytoplasm and to avoid degradation, viruses have to escape this endoso...

journal_title：Journal of cell science

authors： Staring J,Raaben M,Brummelkamp TR

更新日期：2018-08-03 00:00:00

abstract：:Interactions between melanocytes and neighboring cells in the skin are important in regulating skin color in humans. We recently demonstrated that the less pigmented and thicker skin on the palms and soles is regulated by underlying fibroblasts in those areas, specifically via a secreted factor (DKK1) that modulates W...

journal_title：Journal of cell science

authors： Choi W,Wolber R,Gerwat W,Mann T,Batzer J,Smuda C,Liu H,Kolbe L,Hearing VJ

更新日期：2010-09-15 00:00:00

abstract：:In primary cultures of rat cerebellar granule cells with a functional network of glutamatergic neurons, the expression pattern of the different subunits of nitric-oxide (NO)-sensitive guanylyl cyclase changes during cell differentiation. These cells express the alpha1, alpha2 and beta1 subunits of NO-sensitive guanyly...

journal_title：Journal of cell science

authors： Jurado S,Sanchez-Prieto J,Torres M

更新日期：2003-08-01 00:00:00

abstract：:The distribution of anionic sites on the membranes of rat pancreatic B cells and of their storage granules has been studied by the use of a visual probe of cationic ferritin. Membranes of isolated storage granules possessed a net negative charge which was apparently evenly distributed; the number of anionic sites was ...

journal_title：Journal of cell science

authors： Howell SL,Tyhurst M

更新日期：1977-01-01 00:00:00

abstract：:Ligand-dependent nuclear import is crucial for the function of the androgen receptor (AR) in both health and disease. The unliganded AR is retained in the cytoplasm but, on binding 5alpha-dihydrotestosterone, it translocates into the nucleus and alters transcription of its target genes. Nuclear import of AR is mediate...

journal_title：Journal of cell science

authors： Cutress ML,Whitaker HC,Mills IG,Stewart M,Neal DE

更新日期：2008-04-01 00:00:00

abstract：:The weak spindle integrity checkpoint in Drosophila spermatocytes has revealed a novel function of the gamma-tubulin ring complex (gammaTuRC) in maintaining spindle bipolarity throughout meiosis. Bipolar and bi-astral spindles could form in Drosophila mutants for dd4, the gene encoding the 91 kDa subunit of gammaTuRC....

journal_title：Journal of cell science

authors： Barbosa V,Gatt M,Rebollo E,Gonzalez C,Glover DM

更新日期：2003-03-01 00:00:00

abstract：:Xenopus embryos initiate replication at random closely spaced sites until a certain concentration of nuclei is achieved within the embryo, after which fewer, more specific chromosomal sites are utilized as origins. We have examined the relationship between nucleo-cytosolic ratio and origin specification when Chinese h...

journal_title：Journal of cell science

authors： Dimitrova DS,Gilbert DM

更新日期：1998-10-01 00:00:00

abstract：:More than 15 subunits of the integrin family of cell surface adhesion molecules have been identified. The alpha 6 beta 4 integrin has recently been identified as a component of hemidesmosomes of stratified squamous epithelium. The monoclonal antibody (mAb) 346-11A binds to the beta 4 subunit in mice. Sequence analysis...

journal_title：Journal of cell science

authors： Kennel SJ,Godfrey V,Ch'ang LY,Lankford TK,Foote LJ,Makkinje A

更新日期：1992-01-01 00:00:00

abstract：:Rapid endocytosis of lipoprotein receptors involves NPxY signals contained in their cytoplasmic tails. Several proteins, including ARH and Dab2, can bind these sequences, but their importance for endocytosis may vary in different cell types. The lipoprotein receptor megalin is expressed in the visceral endoderm (VE), ...

journal_title：Journal of cell science

authors： Maurer ME,Cooper JA

更新日期：2005-11-15 00:00:00

abstract：:We use the fungus Ashbya gossypii to investigate how its polar growth machinery is organized to achieve sustained hyphal growth. In slowly elongating hyphae exocyst, cell polarity and polarisome proteins permanently localize as cortical cap at hyphal tips, thus defining the zone of secretory vesicle fusion. In tenfold...

journal_title：Journal of cell science

authors： Köhli M,Galati V,Boudier K,Roberson RW,Philippsen P

更新日期：2008-12-01 00:00:00

abstract：:Guinea pig sperm were suspended in calcium-containing medium supplemented with various concentrations of the tetrasaccharide, stachyose. At concentrations up to and including 0.6 M, stachyose was without effect on the A23187-induced acrosome reaction. At 1.0 M stachyose, greater than 97% of sperm retained their acroso...

journal_title：Journal of cell science

authors： Green DP

更新日期：1991-12-01 00:00:00

abstract：:Cadherin has an intimate spatial relationship with actin filaments (AF) in various types of cells, forming the cell-to-cell adherens junction (AJ). We compared the AJ/AF relationship between non-polarized fibroblasts (NRK cells) and polarized epithelial cells (MTD-1A cells). E/P-cadherin, alpha-catenin, ZO-1 and vincu...

journal_title：Journal of cell science

authors： Yonemura S,Itoh M,Nagafuchi A,Tsukita S

更新日期：1995-01-01 00:00:00

abstract：:HEK293 cells expressing wild-type CXCR2 recruit PH-Akt-GFP to the leading edge of the cell in response to chemokine. However, in cells expressing mutant CXCR2 defective in AP-2 and HIP binding, i.e. with a mutation in the LLKIL motif, PH-Akt-GFP does not localize to the leading edge in response to ligand. Inhibition o...

journal_title：Journal of cell science

authors： Sai J,Fan GH,Wang D,Richmond A

更新日期：2004-11-01 00:00:00

abstract：:Meiosis is characterized by two chromosome segregation rounds (meiosis I and II), which follow a single round of DNA replication, resulting in haploid genome formation. Chromosome reduction occurs at meiosis I. It relies on key structures, such as chiasmata, which are formed by repair of double-strand breaks (DSBs) be...

journal_title：Journal of cell science

authors： Meyer RE,Algazeery A,Capri M,Brazier H,Ferry C,Aït-Ahmed O

更新日期：2014-11-01 00:00:00

abstract：:We have reported earlier that the polyphosphoinositide messenger system may control mitosis in sea urchin eggs. Besides phospholipase C activation and its second messengers, phosphatidylinositol (PI) 3-kinase has been proposed to affect a wide variety of cellular processes in other cellular systems. Therefore, we have...

journal_title：Journal of cell science

authors： De Nadai C,Huitorel P,Chiri S,Ciapa B

更新日期：1998-09-01 00:00:00

abstract：:Nerve branching is controlled by intrinsic and extrinsic cues, one of which may be a small applied electric field. Lateral processes were induced by passing current through a micropipette placed at 90 degrees to the shaft of a developing nerve. The appearance of processes was a polarised event with a large majority ar...

journal_title：Journal of cell science

authors： McCaig CD

更新日期：1990-04-01 00:00:00