Abstract:
:We have previously shown that Xenopus oocytes, eggs, and early embryos contain lamins LII and LIII, and that portions of each are associated with distinct egg vesicle populations. We now report that a lamin similar or identical to the B-type lamin LI is also present in oocyte nuclei and in egg extracts. We have quantitated the three B-type lamins per oocyte nucleus, and have calculated relative ratios of LI:LIII = 1:100, and LII:LIII = 1:10. Similar to lamin LII, 5-15% of lamin LI is associated with egg membranes in a biochemically stable manner. Egg vesicles absorbed with lamin isoform-specific antibodies to magnetic beads indicate that lamin LI-associated egg membranes are of heterogenous morphology, and are independent from the lamin LII and LIII vesicle populations. Compared to other nuclear envelope proteins, the synthesis of lamin LI protein is specifically elevated during meiotic maturation, resulting in a 4- to 12-fold higher amount of lamin LI in eggs than is present in oocyte nuclei. Immunofluorescence and immunoblot analysis demonstrated that lamins LI, LII, and LIII are associated with the nuclear envelope formed on demembranated sperm when added to activated egg extract. These results strongly suggest that three different lamin-associated vesicle populations are involved in the formation of a nuclear envelope in egg extracts.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Lourim D,Kempf A,Krohne Gsubject
Has Abstractpub_date
1996-07-01 00:00:00pages
1775-85eissn
0021-9533issn
1477-9137journal_volume
109 ( Pt 7)pub_type
杂志文章abstract::The morphogenesis-related NDR kinase (MOR) pathway regulates morphogenesis in fungi. In spite of the high conservation of its components, impairing their functions results in highly divergent cellular responses depending on the fungal species. The reasons for such differences are unclear. Here we propose that the spec...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.107862
更新日期:2012-10-01 00:00:00
abstract::Cadherin has an intimate spatial relationship with actin filaments (AF) in various types of cells, forming the cell-to-cell adherens junction (AJ). We compared the AJ/AF relationship between non-polarized fibroblasts (NRK cells) and polarized epithelial cells (MTD-1A cells). E/P-cadherin, alpha-catenin, ZO-1 and vincu...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::Membrane-type matrix metalloproteinase 1 (MT1-MMP) is a membrane-anchored matrix metalloproteinase (MMP) that is frequently associated with processes involving tissue remodelling and cell migration. We have examined MT1-MMP expression and subcellular distribution as a function of MCF10A mammary epithelial cell migrati...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-08-01 00:00:00
abstract::Effective cell-to-cell spreading of the facultative intracellular pathogen Listeria monocytogenes requires the interaction between bacteria and the microfilament system of the host cell. By recruiting actin filaments into a 'comet tail' localized at one pole of the bacterial cell wall, Listeria become mobile and prope...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::The interaction of the non-receptor tyrosine kinase, Src, with the cytoskeleton of adhesion sites was studied in nerve growth cones isolated from fetal rat brain. Of particular interest was the role of protein tyrosine phosphatases in the regulation of Src-cytoskeleton binding. Growth cones were found to contain a hig...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
abstract::Most nonmuscle cells of higher vertebrates contain two different actin isoforms, beta- and gamma-cytoplasmic actin. The beta-isoform is with few exceptions the predominant isoform in nonmuscle cells and tissues. Perturbation of the beta:gamma ratio has been shown to affect the organization of bundled actin filaments i...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
abstract::Desmocollin 3 (Dsc3) is a transmembrane glycoprotein that belongs to the cadherin family of cell adhesion receptors. Together with desmoglein(s), it forms the transmembrane core of desmosomes, a multiprotein complex involved in cell adhesion, organization of the cytoskeleton, cell sorting and cell signaling. Previous ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02769
更新日期:2006-02-01 00:00:00
abstract::Kalirin, one of the few Rho guanine nucleotide exchange factors (GEFs) that contains spectrin-like repeats, plays a critical role in axon extension and maintenance of dendritic spines. PC12 cells were used to determine whether Cdk5, a critical participant in both processes, regulates the action of Kalirin. Expression ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.016089
更新日期:2008-08-01 00:00:00
abstract::Organelle transport is vital for the development and maintenance of axons, in which the distances between sites of organelle biogenesis, function, and recycling or degradation can be vast. Movement of mitochondria in axons can serve as a general model for how all organelles move: mitochondria are easy to identify, the...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.02745
更新日期:2005-12-01 00:00:00
abstract::The full-length primary structure and expression profile of a novel unconventional myosin heavy chain, human myosin-IXb, is described. The primary structure of this myosin predicts a 229 kDa protein that together with its recently described rat homolog, myr 5, is the ninth class of myosins to be identified. In compari...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-03-01 00:00:00
abstract::The secreted Frizzled-related proteins (SFRPs) are a family of soluble proteins that are structurally related to Frizzled (Fz) proteins, the serpentine receptors that mediate the extensively used cell-cell communication pathway involving Wnt signalling. Because of their homology with the Wnt-binding domain on the Fz r...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.026096
更新日期:2008-03-15 00:00:00
abstract::Cells are internally organized into compartmentalized organelles that execute specialized functions. To understand the functions of individual organelles and their regulations, it is critical to resolve the compositions of individual organelles, which relies on a rapid and efficient isolation method for specific organ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.235390
更新日期:2019-12-13 00:00:00
abstract::There is a limited number of methods to examine transbilayer lipid distribution in biomembranes. We employed freeze-fracture replica-labelling immunoelectron microscopy in combination with lipid-binding proteins and a peptide to examine both transbilayer distribution and lateral distribution of various phospholipids i...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.163105
更新日期:2015-04-15 00:00:00
abstract::In situ hybridization has become a powerful technique for dissecting nuclear structure. By localizing nucleic acids with high precision, it is possible to infer the native structure of chromosomes, replication factories and transcript processing complexes. To increase the value of this technique, we have established t...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-05-01 00:00:00
abstract::Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3'-OH position of the inositol ring of inositol phospholipids, producing three lipid products: PtdIns(3)P, PtdIns(3,4)P(2) and PtdIns(3,4,5)P(3). These lipids bind to the pleckstrin homology (PH) domains of proteins and control the activity and subcellular localisat...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:
更新日期:2001-04-01 00:00:00
abstract::Rab GTPases are key regulators of intracellular trafficking, and cycle between a GTP-bound active state and a GDP-bound inactive state. This cycle is regulated by guanine-nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). Several efforts have been made in connecting the correct GEFs and GAPs to ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.216630
更新日期:2018-09-03 00:00:00
abstract::Circadian rhythms exist in most living organisms. The general molecular mechanisms that are used to generate 24-hour rhythms are conserved among organisms, although the details vary. These core clocks consist of multiple regulatory feedback loops, and must be coordinated and orchestrated appropriately for the fine-tun...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.065771
更新日期:2011-02-01 00:00:00
abstract::Dystrophin has been proposed to associate with the skeletal muscle membrane by way of a glycoprotein complex that interacts with its C-terminal domains. Transfection of mdx mouse myotubes in culture or myofibres in vivo with recombinant genes encoding human dystrophin deletion mutants shows, however, that not only the...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
abstract::Prion diseases are fatal neurodegenerative diseases characterised by the accumulation of misfolded prion protein (PrP(Sc)) in the brain. They are caused by the templated misfolding of normal cellular protein, PrP(C), by PrP(Sc). We have recently generated a unique cell system in which epitope-tagged PrP(C) competent t...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.120477
更新日期:2013-08-15 00:00:00
abstract::Myofibrillar proteins assemble to form the highly ordered repetitive contractile structural unit known as a sarcomere. Studies of myogenesis in vertebrate cell culture and embryonic developmental systems have identified some of the processes involved during sarcomere formation. However, isoform changes during vertebra...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01024
更新日期:2004-04-01 00:00:00
abstract::During vertebrate gastrulation, a well-orchestrated series of morphogenetic changes leads to the formation of the three germ layers: the ectoderm, mesoderm and endoderm. The analysis of gene expression patterns during gastrulation has been central to the identification of genes involved in germ layer formation. Howeve...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02928
更新日期:2006-05-15 00:00:00
abstract::Caveolins are membrane proteins that are the major coat proteins of caveolae, specialized lipid rafts in the plasma membrane that serve as scaffolding sites for many signaling complexes. Among the many signaling molecules associated with caveolins are the Src tyrosine kinases, whose activation regulates numerous cellu...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00806
更新日期:2003-12-01 00:00:00
abstract::Interaction of the Eph family of receptor protein tyrosine kinases and their ligands, ephrin family members, induces bi-directional signaling via cell-cell contacts. High expression of B-type ephrin is associated with high invasion potential of tumors, however, the mechanism by which ephrin-B promotes cancer cell inva...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.008607
更新日期:2007-07-01 00:00:00
abstract::The specification of organs, tissues and cell types results from cell fate restrictions enacted by nuclear transcription factors under the control of conserved signaling pathways. The progenitor epithelium of the Drosophila compound eye, the eye imaginal disc, is a premier model for the study of such processes. Early ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.237834
更新日期:2020-05-26 00:00:00
abstract::Neutrophil extracellular traps (NETs) are one of the most intriguing discoveries in immunological research of the past few years. After their first description in 2004, the number of research articles on how NETs affect immunodefense, and also how they contribute to an ever-growing number of diseases, has skyrocketed....
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.241075
更新日期:2020-03-10 00:00:00
abstract::A conjugate of horseradish peroxidase (HRP) to poly(L-lysine) (PLL) was used as a non-specific adsorptive probe to study transcytosis in MDCK strain I and Caco-2 epithelial cells. As we have shown previously, HRP-PLL transcytosis proceeds via an intracellular, non-lysosomal proteolytic compartment in MDCK cells; yet, ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
abstract::Mob1 is a component of both the mitotic exit network and Hippo pathway, being required for cytokinesis, control of cell proliferation and apoptosis. Cell division accuracy is crucial in maintaining cell ploidy and genomic stability and relies on the correct establishment of the cell division axis, which is under the c...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.096610
更新日期:2012-01-15 00:00:00
abstract::Fission yeast cells tolerate the total absence of the cdc25 mitotic inducer in two cases, either in cdc2-3w or in wee1 genetic backgrounds. In the cdc2-3w cdc25Delta double mutant, the rate-limiting step leading to mitosis is reaching a critical size. However, the size control of this mutant operates in late G2, which...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::Inactivation of the transcription factor and tumor suppressor p53, and overexpression or mutational activation of PIK3CA, which encodes the p110alpha catalytic subunit of phosphatidylinositol-3-kinase (PI3K), are two of the most common deleterious genomic changes in cancer, including in ovarian carcinomas. We investig...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.013029
更新日期:2008-03-01 00:00:00
abstract::Mdm2 is a nucleoplasmic and nucleolar protein interacting with p53 and alternative reading frame (ARF) tumor suppressor proteins. Here we demonstrate relocalization and novel interactions of Mdm2 with the promyelocytic leukemia (PML) protein following cellular stress and DNA damage. We show that Mdm2 and PML interact ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00714
更新日期:2003-10-01 00:00:00