当前位置： SCI文献检索 > JOURNAL OF CELL SCIENCE期刊下所有文献 > Small hepatocytes in culture develop polarized transporter expression and differentiation.

Small hepatocytes in culture develop polarized transporter expression and differentiation.

Abstract：

:Rat small hepatocytes have been shown to proliferate in culture and to form organoids with differentiated hepatocytes in vitro. To evaluate the degree of polarized transporter differentiation of rat small hepatocytes during 9 weeks of culturing, we studied the time-dependent expression and subcellular localization of the major bile salt and organic anion transport systems of hepatocytes [i.e. the basolateral sodium-taurocholate co-transporting protein (Ntcp), organic-anion-transporting polypeptide 1b2 (Oatp1b2), the canalicular bile-salt export pump (Bsep) and multidrug-resistance-associated protein 2 (Mrp2)]. Small hepatocytes proliferated and differentiated in culture and formed sharply demarcated colonies as assessed by morphology, alpha-fetoprotein, albumin and Mrp1 expression. Polarized surface transporter expression was evident after 5 weeks of culturing for Ntcp, Oatp1b2 and Mrp2, and after 7 weeks for Bsep. After 9 weeks in culture, the vast majority of matured hepatocytes expressed Ntcp/Oatp1b2 at the basolateral and Bsep/Mrp2 at the canalicular plasma-membrane domains. This polarized transporter expression was accompanied by canalicular secretion of fluorescein-diacetate and cholylglycyl-fluorescein. Furthermore, an anastomizing three-dimensional network of bile canaliculi developed within piling-up colonies. These data demonstrate that cultured rat small hepatocytes acquire a fully differentiated transporter expression phenotype during their development into hepatic 'organoid-like' clusters of mature hepatocytes. Thereby, the time-dependent sequence of transporter expression mirrored the ontogenesis of transporter expression in developing rat liver, supporting the concept that small hepatocytes correspond to the hepatocyte lineage derived from embryonic hepatoblasts and/or from a different pool of 'committed hepatocyte progenitor cells'.

journal_name

J Cell Sci

journal_title

Journal of cell science

authors

Sidler Pfändler MA,Höchli M,Inderbitzin D,Meier PJ,Stieger B

doi

10.1242/jcs.01279

keywords:

subject

Has Abstract

pub_date

2004-08-15 00:00:00

pages

4077-87

issue

Pt 18

eissn

0021-9533

issn

1477-9137

pii

jcs.01279

journal_volume

117

pub_type

杂志文章

相关文献

JOURNAL OF CELL SCIENCE文献大全
  • Lentivirus-mediated transduction of connexin cDNAs shows level- and isoform-specific alterations in insulin secretion of primary pancreatic beta-cells.

    abstract：:We have generated novel lentiviral vectors to integrate various connexin cDNAs into primary, non-dividing cells. We have used these vectors to test whether proper control of insulin secretion depends on a specific connexin isoform and/or on its level of expression. We have observed that transduced connexin32, connexin...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.00442

    authors： Caton D,Calabrese A,Mas C,Serre-Beinier V,Charollais A,Caille D,Zufferey R,Trono D,Meda P

    更新日期：2003-06-01 00:00:00

  • Microtubule-associated protein-4 controls nanovesicle dynamics and T cell activation.

    abstract：:The immune synapse (IS) is a specialized structure formed at the contact area between T lymphocytes and antigen-presenting cells (APCs) that is essential for the adaptive immune response. Proper T cell activation requires its polarization towards the APC, which is highly dependent on the tubulin cytoskeleton. Microtub...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.199042

    authors： Bustos-Morán E,Blas-Rus N,Martin-Cófreces NB,Sánchez-Madrid F

    更新日期：2017-04-01 00:00:00

  • FAK regulates actin polymerization during sperm capacitation via the ERK2/GEF-H1/RhoA signaling pathway.

    abstract：:Actin polymerization is a crucial process during sperm capacitation. We have recently described the participation of FAK during actin polymerization in guinea pig spermatozoa. However, the mechanism by which FAK mediates these processes is unknown. Our previous data have shown that MAPK1 (hereafter referred to as ERK2...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.239186

    authors： Salgado-Lucio ML,Ramírez-Ramírez D,Jorge-Cruz CY,Roa-Espitia AL,Hernández-González EO

    更新日期：2020-04-28 00:00:00

  • Single-molecule analysis of chemoattractant-stimulated membrane recruitment of a PH-domain-containing protein.

    abstract：:Molecular mechanisms of chemotactic response are highly conserved among many eukaryotic cells including human leukocytes and Dictyostelium discoideum cells. The cells can sense the differences in chemoattractant concentration across the cell body and respond by extending pseudopods from the cell side facing to a highe...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.02824

    authors： Matsuoka S,Iijima M,Watanabe TM,Kuwayama H,Yanagida T,Devreotes PN,Ueda M

    更新日期：2006-03-15 00:00:00

  • Calpain inhibitors, but not caspase inhibitors, prevent actin proteolysis and DNA fragmentation during apoptosis.

    abstract：:Apoptosis, or programmed cell death, involves a cascade of regulatory events leading to the activation of specific proteases. However, the key substrates for these proteases remain to be identified. We previously demonstrated that levels of five unidentified polypeptides were specifically increased in neurons from emb...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Villa PG,Henzel WJ,Sensenbrenner M,Henderson CE,Pettmann B

    更新日期：1998-03-01 00:00:00

  • Active RNA polymerases are localized within discrete transcription "factories' in human nuclei.

    abstract：:Nascent transcripts in permeabilized HeLa cells were elongated by approximately 30-2,000 nucleotides in Br-UTP or biotin-14-CTP, before incorporation sites were immunolabelled either pre- or post-embedding, and visualized by light or electron microscopy. Analogues were concentrated in approximately 2,100 (range 2,000-...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Iborra FJ,Pombo A,Jackson DA,Cook PR

    更新日期：1996-06-01 00:00:00

  • Salt-stable interaction of the amino-terminal head region of vimentin with the alpha-helical rod domain of cytoplasmic intermediate filament proteins and its relevance to protofilament structure and filament formation and stability.

    abstract：:Previous studies have shown that the non-alpha-helical, amino-terminal head region of vimentin is essential for the formation and stability of vimentin intermediate filaments (IFs). In order to specify its target site on companion protein subunits, it was cut off from vimentin at amino acid position 96 with lysine-spe...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Traub P,Scherbarth A,Wiegers W,Shoeman RL

    更新日期：1992-02-01 00:00:00

  • Infiltration and survival: the behaviour of normal, invasive cells implanted to the developing chick wing.

    abstract：:The invasiveness of mouse lymphocytes and thymocytes, rabbit peritoneal neutrophil granulocytes (PMNs), mouse peritoneal macrophages (both activated and non-activated) and pig endothelial cells was assayed by implanting these cells to the chick wing bud. Cells of each type moved into the wing mesenchyme, although acti...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Tickle C,Crawley A

    更新日期：1979-12-01 00:00:00

  • Functional properties of proximal tubule cell lines derived from transgenic mice harboring L-pyruvate kinase-SV40 (T) antigen hybrid gene.

    abstract：:This study describes the functional characterization of two cell lines derived from the proximal convoluted (PKSV-PCT cells) and proximal straight (PKSV-PR) tubules microdissected out from kidneys of transgenic mice harboring the simian virus 40 (SV40) large T and small t antigens placed under the control of the rat L...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Lacave R,Bens M,Cartier N,Vallet V,Robine S,Pringault E,Kahn A,Vandewalle A

    更新日期：1993-03-01 00:00:00

  • Troponin I is required for myofibrillogenesis and sarcomere formation in Drosophila flight muscle.

    abstract：:Myofibrillar proteins assemble to form the highly ordered repetitive contractile structural unit known as a sarcomere. Studies of myogenesis in vertebrate cell culture and embryonic developmental systems have identified some of the processes involved during sarcomere formation. However, isoform changes during vertebra...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.01024

    authors： Nongthomba U,Clark S,Cummins M,Ansari M,Stark M,Sparrow JC

    更新日期：2004-04-01 00:00:00

  • CENP-B creates alternative epigenetic chromatin states permissive for CENP-A or heterochromatin assembly.

    abstract：:CENP-B binds to CENP-B boxes on centromeric satellite DNAs (known as alphoid DNA in humans). CENP-B maintains kinetochore function through interactions with CENP-A nucleosomes and CENP-C. CENP-B binding to transfected alphoid DNA can induce de novo CENP-A assembly, functional centromere and kinetochore formation, and ...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.243303

    authors： Otake K,Ohzeki JI,Shono N,Kugou K,Okazaki K,Nagase T,Yamakawa H,Kouprina N,Larionov V,Kimura H,Earnshaw WC,Masumoto H

    更新日期：2020-08-11 00:00:00

  • Differential expression of NO-sensitive guanylyl cyclase subunits during the development of rat cerebellar granule cells: regulation via N-methyl-D-aspartate receptors.

    abstract：:In primary cultures of rat cerebellar granule cells with a functional network of glutamatergic neurons, the expression pattern of the different subunits of nitric-oxide (NO)-sensitive guanylyl cyclase changes during cell differentiation. These cells express the alpha1, alpha2 and beta1 subunits of NO-sensitive guanyly...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.00620

    authors： Jurado S,Sanchez-Prieto J,Torres M

    更新日期：2003-08-01 00:00:00

  • Microautophagy - distinct molecular mechanisms handle cargoes of many sizes.

    abstract：:Autophagy is fundamental for cell and organismal health. Two types of autophagy are conserved in eukaryotes: macroautophagy and microautophagy. During macroautophagy, autophagosomes deliver cytoplasmic constituents to endosomes or lysosomes, whereas during microautophagy lytic organelles take up cytoplasm directly. Wh...

    journal_title：Journal of cell science

    pub_type： 杂志文章,评审

    doi：10.1242/jcs.246322

    authors： Schuck S

    更新日期：2020-09-09 00:00:00

  • FIB/SEM-based analysis of Borrelia intracellular processing by human macrophages.

    abstract：:Borrelia burgdorferi is the causative agent of Lyme disease, a multisystemic disorder affecting primarily skin, joints and nervous system. Successful internalization and intracellular processing of borreliae by immune cells like macrophages is decisive for the outcome of a respective infection. Here, we use for the fi...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.252320

    authors： Klose M,Scheungrab M,Luckner M,Wanner G,Linder S

    更新日期：2020-12-30 00:00:00

  • Growth-speed-correlated localization of exocyst and polarisome components in growth zones of Ashbya gossypii hyphal tips.

    abstract：:We use the fungus Ashbya gossypii to investigate how its polar growth machinery is organized to achieve sustained hyphal growth. In slowly elongating hyphae exocyst, cell polarity and polarisome proteins permanently localize as cortical cap at hyphal tips, thus defining the zone of secretory vesicle fusion. In tenfold...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.033852

    authors： Köhli M,Galati V,Boudier K,Roberson RW,Philippsen P

    更新日期：2008-12-01 00:00:00

  • Cathepsin L protease (CPL-1) is essential for yolk processing during embryogenesis in Caenorhabditis elegans.

    abstract：:Cysteine proteases are involved in the degradation of intracellular and extracellular proteins, although their precise roles in vivo are not well understood. Here we characterise a genetic mutant of the Caenorhabditis elegans cathepsin L protease gene cpl-1. CPL-1 is provided maternally and is essential for C. elegans...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.01387

    authors： Britton C,Murray L

    更新日期：2004-10-01 00:00:00

  • Reactive oxygen species exert opposite effects on Tyr23 phosphorylation of the nuclear and cortical pools of annexin A2.

    abstract：:Annexin A2 (AnxA2) is a multi-functional and -compartmental protein whose subcellular localisation and functions are tightly regulated by its post-translational modifications. AnxA2 and its Tyr23-phosphorylated form (pTyr23AnxA2) are involved in malignant cell transformation, metastasis and angiogenesis. Here, we show...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.173195

    authors： Grindheim AK,Hollås H,Raddum AM,Saraste J,Vedeler A

    更新日期：2016-01-15 00:00:00

  • Nucleolar localization of the human telomeric repeat binding factor 2 (TRF2).

    abstract：:The telomeric repeat binding factor 2 (TRF2) specifically recognizes TTAGGG tandem repeats at chromosomal ends. Unexpectedly immunofluorescence studies revealed a prominent nucleolar localization of TRF2 in human cells, which appeared as discrete dots with sizes similar to those present in the nucleoplasm. The TRF2 do...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.01249

    authors： Zhang S,Hemmerich P,Grosse F

    更新日期：2004-08-01 00:00:00

  • CAF-1 and the inheritance of chromatin states: at the crossroads of DNA replication and repair.

    abstract：:Chromatin is no longer considered to be a static structural framework for packaging DNA within the nucleus but is instead believed to be an interactive component of DNA metabolism. The ordered assembly of chromatin produces a nucleoprotein template capable of epigenetically regulating the expression and maintenance of...

    journal_title：Journal of cell science

    pub_type： 杂志文章,评审

    doi：

    authors： Ridgway P,Almouzni G

    更新日期：2000-08-01 00:00:00

  • A quantitative analysis of connexin-specific permeability differences of gap junctions expressed in HeLa transfectants and Xenopus oocytes.

    abstract：:Gap junctions provide direct intercellular communication by linking adjacent cells with aqueous pores permeable to molecules up to 1 kDa in molecular mass and 8-14 A in diameter. The identification of over a dozen connexins in the mammalian gap junction family has stimulated interest in the functional significance of ...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Cao F,Eckert R,Elfgang C,Nitsche JM,Snyder SA,H-ulser DF,Willecke K,Nicholson BJ

    更新日期：1998-01-01 00:00:00

  • Regulation of multiple target genes by miR-1 and miR-206 is pivotal for C2C12 myoblast differentiation.

    abstract：:MicroRNAs are short non-coding RNAs involved in post-transcriptional regulation of multiple messenger RNA targets. The miR-1/miR-206 family is expressed during skeletal muscle differentiation and is an integral component of myogenesis. To better understand miR-1/miR-206 function during myoblast differentiation we iden...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.101758

    authors： Goljanek-Whysall K,Pais H,Rathjen T,Sweetman D,Dalmay T,Münsterberg A

    更新日期：2012-08-01 00:00:00

  • Acute HSF1 depletion induces cellular senescence through the MDM2-p53-p21 pathway in human diploid fibroblasts.

    abstract：:Heat shock transcription factor 1 (HSF1) regulates the expression of a wide array of genes, controls the expression of heat shock proteins (HSPs) as well as cell growth. Although acute depletion of HSF1 induces cellular senescence, the underlying mechanisms are poorly understood. Here, we report that HSF1 depletion-in...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.210724

    authors： Oda T,Sekimoto T,Kurashima K,Fujimoto M,Nakai A,Yamashita T

    更新日期：2018-05-08 00:00:00

  • Ultrastructure of the dinoflagellate Polykrikos. I. Development of the nematocyst-taeniocyst complex and morphology of the site for extrusion.

    abstract：:Development of the nematocyst-taeniocyst complex in the four-zooid stage of a dinoflagellate, Polykrikos kofoidi, was studied by electron microscopy. We observed the following stages: formation of large spherical bodies in islets of cytoplasm containing extensive rough endoplasmic reticulum and Golgi complexes; differ...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Westfall JA,Bradbury PC,Townsend JW

    更新日期：1983-09-01 00:00:00

  • Deleterious assembly of the lamin A/C mutant p.S143P causes ER stress in familial dilated cardiomyopathy.

    abstract：:Mutation of the LMNA gene, encoding nuclear lamin A and lamin C (hereafter lamin A/C), is a common cause of familial dilated cardiomyopathy (DCM). Among Finnish DCM patients, the founder mutation c.427T>C (p.S143P) is the most frequently reported genetic variant. Here, we show that p.S143P lamin A/C is more nucleoplas...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.184150

    authors： West G,Gullmets J,Virtanen L,Li SP,Keinänen A,Shimi T,Mauermann M,Heliö T,Kaartinen M,Ollila L,Kuusisto J,Eriksson JE,Goldman RD,Herrmann H,Taimen P

    更新日期：2016-07-15 00:00:00

  • Cep57 and Cep57l1 function redundantly to recruit the Cep63-Cep152 complex for centriole biogenesis.

    abstract：:The Cep63-Cep152 complex located at the mother centriole recruits Plk4 to initiate centriole biogenesis. How the complex is targeted to mother centrioles, however, is unclear. In this study, we show that Cep57 and its paralog, Cep57l1, colocalize with Cep63 and Cep152 at the proximal end of mother centrioles in both c...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.241836

    authors： Zhao H,Yang S,Chen Q,Duan X,Li G,Huang Q,Zhu X,Yan X

    更新日期：2020-07-03 00:00:00

  • Isolation of the human genes encoding the pyst1 and Pyst2 phosphatases: characterisation of Pyst2 as a cytosolic dual-specificity MAP kinase phosphatase and its catalytic activation by both MAP and SAP kinases.

    abstract：:We have isolated the human genes encoding the Pyst1 (MKP-3) and Pyst2 (MKP-X) MAP kinase phosphatases. Both genes consist of three exons interrupted by two introns and lack an intron which is conserved in all the other members of this gene family characterised to date. This reinforces the conclusion that Pyst1 and Pys...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Dowd S,Sneddon AA,Keyse SM

    更新日期：1998-11-01 00:00:00

  • Microtubule depolymerization induces traction force increase through two distinct pathways.

    abstract：:Traction forces increase after microtubule depolymerization; however, the signaling mechanisms underlying this, in particular the dependence upon myosin II, remain unclear. We investigated the mechanism of traction force increase after nocodazole-induced microtubule depolymerization by applying traction force microsco...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.090563

    authors： Rape A,Guo WH,Wang YL

    更新日期：2011-12-15 00:00:00

  • Modeling the self-organized phosphatidylinositol lipid signaling system in chemotactic cells using quantitative image analysis.

    abstract：:A key signaling event that is responsible for gradient sensing in eukaryotic cell chemotaxis is a phosphatidylinositol (PtdIns) lipid reaction system. The self-organization activity of this PtdIns lipid system induces an inherent polarity, even in the absence of an external chemoattractant gradient, by producing a loc...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.108373

    authors： Shibata T,Nishikawa M,Matsuoka S,Ueda M

    更新日期：2012-11-01 00:00:00

  • IPS-1 plays an essential role in dsRNA-induced stress granule formation by interacting with PKR and promoting its activation.

    abstract：:The formation of cytoplasmic stress granules and the innate immune response are two distinct cellular stress responses. Our study investigated the involvement of four innate immune proteins - retinoic-acid-inducible gene I (RIG-I, also known as DDX58), melanoma differentiation-associated gene 5 (MDA5, also known as IF...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.139626

    authors： Zhang P,Li Y,Xia J,He J,Pu J,Xie J,Wu S,Feng L,Huang X,Zhang P

    更新日期：2014-06-01 00:00:00

  • Pom1 kinase links division plane position to cell polarity by regulating Mid1p cortical distribution.

    abstract：:In fission yeast, Mid1p, a major determinant for division plane position, defines a medial cortical compartment where it recruits myosin II at the onset of mitosis to initiate contractile ring assembly. How Mid1p is restricted to the medial cortex is unknown. We report here that in a pom1 polarity mutant, which displa...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.03261

    authors： Celton-Morizur S,Racine V,Sibarita JB,Paoletti A

    更新日期：2006-11-15 00:00:00