Abstract:
:Cysteine proteases are involved in the degradation of intracellular and extracellular proteins, although their precise roles in vivo are not well understood. Here we characterise a genetic mutant of the Caenorhabditis elegans cathepsin L protease gene cpl-1. CPL-1 is provided maternally and is essential for C. elegans embryogenesis. Immunofluorescence and electron microscopy data show that yolk endocytosis and initial yolk platelet formation occur normally in cpl-1 mutant oocytes and embryos. However, at around the 8-12 cell stage of embryogenesis, yolk platelets begin to aggregate and these enlarged yolk platelets fill the cytoplasm of cpl-1 mutant embryos. Coincident with this aggregation is loss of fluorescence from a yolk green fluorescent protein (YP170::GFP). This suggests that loss of CPL-1 activity leads to aberrant processing and/or conformational changes in yolk proteins, resulting in abnormal platelet fusion. This study has relevance to the abnormal fusion and aggregation of lysosomes in cathepsin L-deficient mice and to other lysosomal disorders.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Britton C,Murray Ldoi
10.1242/jcs.01387keywords:
subject
Has Abstractpub_date
2004-10-01 00:00:00pages
5133-43issue
Pt 21eissn
0021-9533issn
1477-9137pii
117/21/5133journal_volume
117pub_type
杂志文章abstract::Xenopus egg extracts, which support nuclear assembly and DNA replication, were functionally depleted of lamin LIII by inoculating them with monoclonal anti-lamin antibodies. Phase-contrast microscopy and electron-microscopy studies indicated that lamin-depleted extracts supported efficient chromatin decondensation, an...
journal_title:Journal of cell science
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更新日期:1991-03-01 00:00:00
abstract::A comparison of stained cytosolic proteins, in vivo 80 S ribosome translation products and in vitro translation products of poly(A)+ RNA from three species of Acetabularia was performed after characterization of their molecular weights and isoelectric points via two-dimensional electrophoresis. A total of 803 stained ...
journal_title:Journal of cell science
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更新日期:1983-03-01 00:00:00
abstract::Little is known about the molecular mechanisms that regulate the organization of vascular lumen. In this paper we show that lumen formation correlates with endothelial polarization. Adherens junctions (AJs) and VE-cadherin (VEC, encoded by CDH5) are required for endothelial apicobasal polarity in vitro and during embr...
journal_title:Journal of cell science
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更新日期:2010-04-01 00:00:00
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doi:10.1242/jcs.075275
更新日期:2010-11-01 00:00:00
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更新日期:2013-06-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
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更新日期:2003-06-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1976-11-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2020-11-05 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1988-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1983-09-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:
更新日期:2000-01-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1979-04-01 00:00:00
abstract::Differentially expressed membrane antigens have been used to flow-sort viable luminal epithelial and myoepithelial cells from freshly disaggregated adult virgin rat mammary parenchyma. Resulting cultures and clones have been characterized morphologically and by a panel of antibodies that recognise cell-type-specific c...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1991-11-01 00:00:00
abstract::The atomic force microscope (AFM) was used to directly image hippocampal neurons and glia. Using chemically fixed and living cells it was possible to reconstruct three-dimensional cell structure and detect sub-cellular features such as the nucleus, mitochondria and filaments. By repeatedly scanning a single living cel...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract::In somatic cells, the heterodimeric Structural Maintenance of Chromosomes (SMC) proteins are involved in chromosome condensation and gene dosage compensation (SMC2 and 4), and sister chromatid cohesion and DNA recombination (SMC1 and 3). We report here evidence for an involvement of mammalian SMC1 and SMC3 proteins in...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
abstract::Yeast Reb1 and its mammalian ortholog TTF1 are conserved Myb-type DNA-binding proteins that bind to specific sites near the 3'-end of rRNA genes (rDNA). Here, they participate in the termination of transcription driven by RNA polymerase I and block DNA replication forks approaching in the opposite direction. We found ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.070987
更新日期:2011-01-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1990-02-01 00:00:00
abstract::The role of basal epithelial cells in prostatic function, development and carcinogenesis is unknown. The ability of basal prostatic epithelial cells to acquire a luminal phenotype was explored in vitro using the NRP-152 rat dorsal-lateral prostate epithelial cell line as a model system. NRP-152, which was spontaneousl...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::Neural progenitors in the vertebrate nervous system are fully polarized epithelial cells, with intercellular junctions at the apical region. These progenitor cells remain within the neuroepithelium throughout neurogenesis, and will ultimately give rise to all the neurons in the mature nervous system. We have addressed...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03170
更新日期:2006-10-15 00:00:00
abstract::Calnexin and calreticulin are related proteins that comprise an ER chaperone system that ensures the proper folding and quality control of newly synthesized glycoproteins. The specificity for glycoproteins is conferred by a lectin site that recognizes an early oligosaccharide processing intermediate on the folding gly...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
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更新日期:2006-02-15 00:00:00
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journal_title:Journal of cell science
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更新日期:2008-03-15 00:00:00
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journal_title:Journal of cell science
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doi:
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journal_title:Journal of cell science
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doi:
更新日期:1994-12-01 00:00:00
abstract::The human adenocarcinoma cell line Caco-2 was used for studies on the biosynthesis and transport of lysosomal acid alpha-glucosidase in polarized epithelial cells. Metabolic labelling revealed that in Caco-2 cells alpha-glucosidase is synthesized as a precursor form of 110 x 10(3) Mr. This form is converted into a pre...
journal_title:Journal of cell science
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更新日期:1991-10-01 00:00:00
abstract::The co-repressor proteins SMRT and NCoR concentrate in specific subnuclear compartments and function with DNA-binding factors to inhibit transcription. To provide detailed mechanistic understanding of these activities, this study tested the hypothesis that functional interactions with transcription factors, such as th...
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更新日期:2005-08-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.059196
更新日期:2009-11-15 00:00:00
abstract::Prostaglandins E1, E2, and F2alpha (PGE1, PGE2, and PGF2alpha) were shown to inhibit the growth of mouse leukaemia lymphoblasts L5178Y in culture. The effects of PGE1 and PGE2 were greater than that of PGF2alpha. PGE1 and PGE2, at the concentration of 100 mug per ml showed significant inhibitory effects on the rates o...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1976-01-01 00:00:00
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更新日期:2000-02-01 00:00:00