Abstract:
:Little is known about the molecular mechanisms that regulate the organization of vascular lumen. In this paper we show that lumen formation correlates with endothelial polarization. Adherens junctions (AJs) and VE-cadherin (VEC, encoded by CDH5) are required for endothelial apicobasal polarity in vitro and during embryonic development. Silencing of CDH5 gene expression leads to abrogation of endothelial polarity accompanied by strong alterations in lumenal structure. VEC co-distributes with members of the Par polarity complex (Par3 and PKCzeta) and is needed for activation of PKCzeta. CCM1 is encoded by the CCM1 gene, which is mutated in 60% of patients affected by cerebral cavernous malformation (CCM). The protein interacts with VEC and directs AJ organization and AJ association with the polarity complex, both in cell-culture models and in human CCM1 lesions. Both VEC and CCM1 control Rap1 concentration at cell-cell junctions. We propose that VEC, CCM1 and Rap1 form a signaling complex. In the absence of any of these proteins, AJs are dismantled, cell polarity is lost and vascular lumenal structure is severely altered.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Lampugnani MG,Orsenigo F,Rudini N,Maddaluno L,Boulday G,Chapon F,Dejana Edoi
10.1242/jcs.059329subject
Has Abstractpub_date
2010-04-01 00:00:00pages
1073-80issue
Pt 7eissn
0021-9533issn
1477-9137pii
123/7/1073journal_volume
123pub_type
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