Biosynthesis and transport of lysosomal alpha-glucosidase in the human colon carcinoma cell line Caco-2: secretion from the apical surface.

abstract：:We previously reported that, among the three connexins expressed in chick lens, overexpression of connexin (Cx) 45.6, not Cx43 or Cx56, stimulates lens cell differentiation; however, the underlying mechanism responsible for this effect is unclear. Here, we took advantage of naturally occurring loss-of-gap-junction fun...

journal_title：Journal of cell science

authors： Banks EA,Yu XS,Shi Q,Jiang JX

更新日期：2007-10-15 00:00:00

abstract：:Hemidesmosomes are epithelial-specific attachment structures that maintain tissue integrity and resist tension. Despite their importance, how hemidesmosomes are regulated at the post-transcriptional level is poorly understood. Caenorhabditiselegans hemidesmosomes (CeHDs) have a similar structure and composition to the...

journal_title：Journal of cell science

authors： Fu R,Zhu Y,Jiang X,Li Y,Zhu M,Dong M,Huang Z,Wang C,Labouesse M,Zhang H

更新日期：2018-06-11 00:00:00

abstract：:Adhesion between cells is established by the formation of specialized intercellular junctional complexes, such as desmosomes. Desmosomes contain isoforms of two members of the cadherin superfamily of cell adhesion proteins, desmocollins (Dsc) and desmogleins (Dsg), but their combinatorial roles in desmosome assembly a...

journal_title：Journal of cell science

authors： Lowndes M,Rakshit S,Shafraz O,Borghi N,Harmon RM,Green KJ,Sivasankar S,Nelson WJ

更新日期：2014-05-15 00:00:00

abstract：:The presence of an acetolysis-resistant polymer (sporopollenin) in the cellular slime moulds is demonstrated. This polymer is located on the stalk sheath of fruiting bodies as a bundle of fine fibrils (4-5 nm diameter). The location and structure of sporopollenin in spores are shown to vary considerably, depending upo...

journal_title：Journal of cell science

authors： Maeda Y

更新日期：1984-03-01 00:00:00

abstract：:Phosphoinositides regulate myriad cellular processes, acting as potent signaling molecules in conserved signaling pathways and as organelle gatekeepers that recruit effector proteins to membranes. Phosphoinositide-generating enzymes have been studied extensively in yeast and cultured cells, yet their roles in animal d...

journal_title：Journal of cell science

authors： Tan J,Oh K,Burgess J,Hipfner DR,Brill JA

更新日期：2014-03-01 00:00:00

abstract：:HSP27 and alphaB-crystallin are both members of the small heat shock protein family. alphaB-crystalllin has been proposed to modulate intermediate filaments and recently a mutation in alphaB-crystallin has been identified as the genetic basis of desmin related myopathy. This disease is characterised in its pathology b...

journal_title：Journal of cell science

authors： Perng MD,Cairns L,van den IJssel P,Prescott A,Hutcheson AM,Quinlan RA

更新日期：1999-07-01 00:00:00

abstract：:Cardiac fibrosis is linked to fibroblast-to-myofibroblast phenoconversion and proliferation but the mechanisms underlying this are poorly understood. Ski is a negative regulator of TGF-β-Smad signaling in myofibroblasts, and might redirect the myofibroblast phenotype back to fibroblasts. Meox2 could alter TGF-β-mediat...

journal_title：Journal of cell science

authors： Cunnington RH,Northcott JM,Ghavami S,Filomeno KL,Jahan F,Kavosh MS,Davies JJ,Wigle JT,Dixon IM

更新日期：2014-01-01 00:00:00

abstract：:CDC6, a replication licensing protein, is partially exported to the cytoplasm in human cells through phosphorylation by Cdk during S phase, but a significant proportion remains in the nucleus. We report here that human CDC6 physically interacts with ATR, a crucial checkpoint kinase, in a manner that is stimulated by p...

journal_title：Journal of cell science

authors： Yoshida K,Sugimoto N,Iwahori S,Yugawa T,Narisawa-Saito M,Kiyono T,Fujita M

更新日期：2010-01-15 00:00:00

abstract：:Accumulation of adipocytes and collagen type-I-producing cells (fibrosis) is observed in muscular dystrophies. The origin of these cells had been largely unknown, but recently we identified mesenchymal progenitors positive for platelet-derived growth factor receptor alpha (PDGFRα) as the origin of adipocytes in skelet...

journal_title：Journal of cell science

authors： Uezumi A,Ito T,Morikawa D,Shimizu N,Yoneda T,Segawa M,Yamaguchi M,Ogawa R,Matev MM,Miyagoe-Suzuki Y,Takeda S,Tsujikawa K,Tsuchida K,Yamamoto H,Fukada S

更新日期：2011-11-01 00:00:00

abstract：:We have discovered a novel mitosis-specific human autoantigen that arises at the centromeres of prophase chromosomes, but ultimately participates in formation of an organelle that bisects the cell at late anaphase and during telophase. The organelle, discernible as a three-dimensional disc by confocal microscopy, enco...

journal_title：Journal of cell science

authors： Andreassen PR,Palmer DK,Wener MH,Margolis RL

更新日期：1991-07-01 00:00:00

abstract：:Neutrophil extracellular traps (NETs) are one of the most intriguing discoveries in immunological research of the past few years. After their first description in 2004, the number of research articles on how NETs affect immunodefense, and also how they contribute to an ever-growing number of diseases, has skyrocketed....

journal_title：Journal of cell science

authors： Neubert E,Meyer D,Kruss S,Erpenbeck L

更新日期：2020-03-10 00:00:00

abstract：:In Saccharomyces cerevisiae, three classes of sphingolipids contain myo-inositol--inositol phosphorylceramide (IPC), mannosylinositol phosphorylceramide (MIPC) and mannosyldiinositol phosphorylceramide [M(IP)(2)C]. No fission yeast equivalent of Ipt1p, the inositolphosphotransferase that synthesizes M(IP)(2)C from MIP...

journal_title：Journal of cell science

authors： Nakase M,Tani M,Morita T,Kitamoto HK,Kashiwazaki J,Nakamura T,Hosomi A,Tanaka N,Takegawa K

更新日期：2010-05-01 00:00:00

abstract：:Mutation of the LMNA gene, encoding nuclear lamin A and lamin C (hereafter lamin A/C), is a common cause of familial dilated cardiomyopathy (DCM). Among Finnish DCM patients, the founder mutation c.427T>C (p.S143P) is the most frequently reported genetic variant. Here, we show that p.S143P lamin A/C is more nucleoplas...

journal_title：Journal of cell science

authors： West G,Gullmets J,Virtanen L,Li SP,Keinänen A,Shimi T,Mauermann M,Heliö T,Kaartinen M,Ollila L,Kuusisto J,Eriksson JE,Goldman RD,Herrmann H,Taimen P

更新日期：2016-07-15 00:00:00

abstract：:Fas ligand (FasL), a potent mediator of apoptosis expressed by CTL and NK cells, is sorted into the inner vesicles of secretory lysosomes for release via exosome-like vesicles. Previous studies identified a proline-rich domain in the cytoplasmic tail required for sorting FasL to secretory lysosomes, but the mechanisms...

journal_title：Journal of cell science

authors： Zuccato E,Blott EJ,Holt O,Sigismund S,Shaw M,Bossi G,Griffiths GM

更新日期：2007-01-01 00:00:00

abstract：:We previously demonstrated that the aspartate protease cathepsin D is activated by ceramide derived from acid sphingomyelinase. Increased expression of cathepsin D in the skin has been reported in wound healing, psoriasis and skin tumors. We explored specific functions of cathepsin D during epidermal differentiation. ...

journal_title：Journal of cell science

authors： Egberts F,Heinrich M,Jensen JM,Winoto-Morbach S,Pfeiffer S,Wickel M,Schunck M,Steude J,Saftig P,Proksch E,Schütze S

更新日期：2004-05-01 00:00:00

abstract：:p97 (CDC-48 in Caenorhabditis elegans) is a ubiquitin-selective AAA (ATPases associated with diverse cellular activities) chaperone and its key function is to disassemble protein complexes. p97 functions in diverse cellular processes including endoplasmic reticulum (ER)-associated degradation, membrane fusion, and mei...

journal_title：Journal of cell science

authors： Sasagawa Y,Otani M,Higashitani N,Higashitani A,Sato K,Ogura T,Yamanaka K

更新日期：2009-10-15 00:00:00

abstract：:There is a limited number of methods to examine transbilayer lipid distribution in biomembranes. We employed freeze-fracture replica-labelling immunoelectron microscopy in combination with lipid-binding proteins and a peptide to examine both transbilayer distribution and lateral distribution of various phospholipids i...

journal_title：Journal of cell science

authors： Murate M,Abe M,Kasahara K,Iwabuchi K,Umeda M,Kobayashi T

更新日期：2015-04-15 00:00:00

abstract：:Low-density lipoprotein (LDL) deposition, aggregation and retention in the endothelial sub-intima are critical initiating events during atherosclerosis. Macrophages digest aggregated LDL (agLDL) through a process called exophagy. High-density lipoprotein (HDL) plays an atheroprotective role, but studies attempting to ...

journal_title：Journal of cell science

authors： Singh RK,Lund FW,Haka AS,Maxfield FR

更新日期：2019-12-02 00:00:00

abstract：:Stress can be temporary or chronic, and mild or acute. Depending on its extent and severity, cells either alter their metabolism, and adopt a new state, or die. Fluctuations in environmental conditions occur frequently, and such stress disturbs cellular homeostasis, but in general, stresses are reversible and last onl...

journal_title：Journal of cell science

authors： Kucherenko MM,Shcherbata HR

更新日期：2018-02-14 00:00:00

abstract：:The Wnt-5a gene encodes a secreted protein that controls several normal processes during embryogenesis and development of adult tissues by as yet unknown mechanisms. Endogenous expression of Wnt-5a mRNA is known to occur in both mouse and human mammary cell lines. To investigate the biological role of Wnt-5a in the hu...

journal_title：Journal of cell science

authors： Jönsson M,Andersson T

更新日期：2001-06-01 00:00:00

abstract：:Axin and conductin (also known as axin2) are structurally related inhibitors of Wnt/β-catenin signalling that promote degradation of β-catenin. Whereas axin is constitutively expressed, conductin is a Wnt target gene implicated in Wnt negative-feedback regulation. Here, we show that axin and conductin differ in their ...

journal_title：Journal of cell science

authors： Bernkopf DB,Hadjihannas MV,Behrens J

更新日期：2015-01-01 00:00:00

abstract：:The type II transmembrane protein CD98, best known as the heavy chain of the heterodimeric amino acid transporters (HAT), is required for the surface expression and basolateral localization of this transporter complex in polarized epithelial cells. CD98 also interacts with beta1 integrins resulting in an increase in t...

journal_title：Journal of cell science

authors： Cai S,Bulus N,Fonseca-Siesser PM,Chen D,Hanks SK,Pozzi A,Zent R

更新日期：2005-03-01 00:00:00

abstract：:The nonclaret disjunctional (ncd) protein is a kinesin-related microtubule motor protein that is encoded at the claret locus in Drosophila and is required for proper chromosome distribution in meiosis and early mitosis. The protein contains a region with 41% amino acid sequence identity to kinesin heavy chain, but tra...

journal_title：Journal of cell science

authors： Chandra R,Endow SA,Salmon ED

更新日期：1993-03-01 00:00:00

abstract：:The serine/threonine protein phosphatase-1 (PP1) complex is a key regulator of the cell cycle. However, the redundancy of PP1 isoforms and the lack of specific inhibitors have hampered studies on the global role of PP1 in cell cycle progression in vertebrates. Here, we show that the overexpression of nuclear inhibitor...

journal_title：Journal of cell science

authors： Winkler C,De Munter S,Van Dessel N,Lesage B,Heroes E,Boens S,Beullens M,Van Eynde A,Bollen M

更新日期：2015-12-15 00:00:00

abstract：:The LEM domain (for lamina-associated polypeptide, emerin, MAN1 domain) defines a group of nuclear proteins that bind chromatin through interaction of the LEM motif with the conserved DNA crosslinking protein, barrier-to-autointegration factor (BAF). Here, we describe a LEM protein annotated in databases as 'Ankyrin r...

journal_title：Journal of cell science

authors： Brachner A,Braun J,Ghodgaonkar M,Castor D,Zlopasa L,Ehrlich V,Jiricny J,Gotzmann J,Knasmüller S,Foisner R

更新日期：2012-02-15 00:00:00

abstract：:The distribution of beta 1,2 N-acetylglucosaminyltransferase I (NAGT I), alpha 1,3-1,6 mannosidase II (Mann II), beta 1,4 galactosyltransferase (GalT), alpha 2,6 sialyltransferase (SialylT) was determined by immuno-labelling of cryo-sections from HeLa cell lines. Antibody labelling in the HeLa cell line was made possi...

journal_title：Journal of cell science

authors： Rabouille C,Hui N,Hunte F,Kieckbusch R,Berger EG,Warren G,Nilsson T

更新日期：1995-04-01 00:00:00

abstract：:Rab proteins comprise a family of monomeric GTPases that control cellular membrane traffic. Rab21 is a poorly characterised member with no known function. Human Rab21 cDNA from K562 cells was subcloned into GFP expression vectors to generate Rab21 and Rab21 mutants defective in either GTP hydrolysis (Rab21 Q78L) or bi...

journal_title：Journal of cell science

authors： Simpson JC,Griffiths G,Wessling-Resnick M,Fransen JA,Bennett H,Jones AT

更新日期：2004-12-15 00:00:00

abstract：:Spindle pole bodies in the fission yeast Schizosaccharomyces pombe are required during meiosis, not only for spindle formation but also for the assembly of forespore membranes. The spo15 mutant is defective in the formation of forespore membranes, which develop into spore envelopes. The spo15(+)gene encodes a protein ...

journal_title：Journal of cell science

authors： Ikemoto S,Nakamura T,Kubo M,Shimoda C

更新日期：2000-02-01 00:00:00

abstract：:The extracellular signal-regulated protein kinase ERK1/2 is a crucial effector linking extracellular stimuli to cellular responses: upon phosphorylation ERK [also known as mitogen-activated protein kinase P42/P44 (MAPK)] concentrates in the nucleus where it activates specific programs of gene expression. Notwithstandi...

journal_title：Journal of cell science

authors： Costa M,Marchi M,Cardarelli F,Roy A,Beltram F,Maffei L,Ratto GM

更新日期：2006-12-01 00:00:00

abstract：:The glucocorticoid receptor and the mineralocorticoid receptor are hormone-dependent transcription factors. They regulate the excitability of rat hippocampus CA1 neurons in a coordinated fashion. We studied the spatial distribution of these transcription factors in nuclei of CA1 neurons by dual labeling immunocytochem...

journal_title：Journal of cell science

authors： van Steensel B,van Binnendijk EP,Hornsby CD,van der Voort HT,Krozowski ZS,de Kloet ER,van Driel R

更新日期：1996-04-01 00:00:00