Abstract:
:The nonclaret disjunctional (ncd) protein is a kinesin-related microtubule motor protein that is encoded at the claret locus in Drosophila and is required for proper chromosome distribution in meiosis and early mitosis. The protein contains a region with 41% amino acid sequence identity to kinesin heavy chain, but translocates on microtubules with the opposite polarity to kinesin, toward microtubule minus ends. The overall structure of ncd also differs from kinesin heavy chain, in that the proposed motor domain is present at the C terminus of the molecule instead of the N terminus, as in kinesin heavy chain. In studies to define the molecular determinants of ncd function, we constructed and expressed a protein with a deletion of the N-terminal 208 amino acids of the non-motor region. Analysis of the truncated protein shows that the protein exhibits microtubule-stimulated Mg(2+)-ATPase activity and binds microtubules in pelleting assays. In contrast to near full-length ncd, the truncated protein does not support directional movement of microtubules in in vitro motility assays. Instead, microtubules show nucleotide-sensitive binding to the truncated protein on glass surfaces and bound microtubules exhibit one-dimensional diffusional movement that is constrained to their longitudinal axis. The diffusional movement reveals a weak binding state of the ncd motor that may represent a mechanochemical intermediate in its ATP hydrolysis cycle. If diffusional movement is a characteristic intrinsic to the claret motor, it is likely to be important in the in vivo function of the protein.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Chandra R,Endow SA,Salmon EDsubject
Has Abstractpub_date
1993-03-01 00:00:00pages
899-906eissn
0021-9533issn
1477-9137journal_volume
104 ( Pt 3)pub_type
杂志文章abstract::The protozoan parasite Giardia intestinalis (also known as Giardia lamblia) is a major waterborne pathogen. During its life cycle, Giardia alternates between the actively growing trophozoite, which has two diploid nuclei with low levels of allelic heterozygosity, and the infectious cyst, which has four nuclei and a to...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.103879
更新日期:2012-05-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
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pub_type: 杂志文章,评审
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更新日期:2018-01-29 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1996-06-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
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pub_type: 杂志文章
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更新日期:2008-04-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-10-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2003-02-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1987-03-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2010-04-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00522
更新日期:2003-07-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1988-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01359
更新日期:2004-09-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00918
更新日期:2003-12-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00234
更新日期:2003-01-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1979-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2012-06-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
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pub_type: 杂志文章
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更新日期:2015-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2009-12-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-06-01 00:00:00
abstract::The existence of two forms of the chromosome passenger complex (CPC) in the mammalian oocyte has meant that its role in female meiosis has remained unclear. Here we use loss- and gain-of function approaches to assess the meiotic functions of one of the shared components of these complexes, INCENP, and of the variable ...
journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2010-12-15 00:00:00
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journal_title:Journal of cell science
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更新日期:2019-08-07 00:00:00
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journal_title:Journal of cell science
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更新日期:2005-10-15 00:00:00
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journal_title:Journal of cell science
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更新日期:2009-06-15 00:00:00