Abstract：
:Motile cilia generate directed hydrodynamic flow that is important for the motility of cells and extracellular fluids. To optimize directed hydrodynamic flow, motile cilia are organized and oriented into a polarized array. Basal bodies (BBs) nucleate and position motile cilia at the cell cortex. Cytoplasmic BB-associated microtubules are conserved structures that extend from BBs. By using the ciliate, Tetrahymena thermophila, combined with EM-tomography and light microscopy, we show that BB-appendage microtubules assemble coincidently with new BB assembly and that they are attached to the cell cortex. These BB-appendage microtubules are specifically marked by post translational modifications of tubulin, including glycylation. Mutations that prevent glycylation shorten BB-appendage microtubules and disrupt BB positioning and cortical attachment. Consistent with the attachment of BB-appendage microtubules to the cell cortex to position BBs, mutations that disrupt the cellular cortical cytoskeleton disrupt the cortical attachment and positioning of BBs. In summary, BB-appendage microtubules promote the organization of ciliary arrays through attachment to the cell cortex.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Junker AD,Soh AWJ,O'Toole ET,Meehl JB,Guha M,Winey M,Honts JE,Gaertig J,Pearson CGdoi
10.1242/jcs.233726subject
Has Abstractpub_date
2019-08-07 00:00:00issue
15eissn
0021-9533issn
1477-9137pii
jcs.233726journal_volume
132pub_type
杂志文章abstract：:The endoplasmic reticulum-Golgi intermediate compartment (ERGIC) defined by the cycling lectin ERGIC-53 consists of tubulovesicular clusters, but it is unknown if these membranes are transport vehicles or stationary entities. Here, we show by live imaging that GFP-ERGIC-53 mainly localizes to long-lived stationary and...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01615
更新日期：2005-01-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1986-06-01 00:00:00
abstract：:The cell surface delivery of extracellular matrix (ECM) and integrins is fundamental for cell migration in wound healing and during cancer cell metastasis. This process is not only driven by several soluble NSF attachment protein (SNAP) receptor (SNARE) proteins, which are key players in vesicle transport at the cell ...
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更新日期：2015-03-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：
更新日期：1997-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：2015-01-01 00:00:00
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pub_type： 杂志文章
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1990-02-01 00:00:00
abstract：:Mucin expression was analysed, in relation to cell growth, in parental HT-29 cells and in two populations of mucus-secreting HT-29 cells selected by adaptation to methotrexate (HT29-MTX) or 5-fluorouracil (HT29-FU). These two populations express mature mucins that differ in their immunoreactivity to antibodies against...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1979-02-01 00:00:00
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更新日期：2020-03-30 00:00:00
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pub_type： 杂志文章
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更新日期：2014-06-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2016-08-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.129668
更新日期：2013-12-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.121988
更新日期：2013-09-15 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1242/jcs.00123
更新日期：2002-12-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:The number of human genetic disorders shown to be due to defects in membrane trafficking has greatly increased during the past five years. Defects have been identified in components involved in sorting of cargo into transport carriers, vesicle budding and scission, movement of vesicles along cytoskeletal tracks, as we...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
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更新日期：2006-12-15 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：1975-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-12-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2001-01-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2008-05-15 00:00:00
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pub_type： 杂志文章
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2001-11-01 00:00:00
