GAS2-like proteins mediate communication between microtubules and actin through interactions with end-binding proteins.

abstract：:The biosynthetic machinery in the melanotrope cells of the Xenopus intermediate pituitary is primarily dedicated to the generation of proopiomelanocortin (POMC)-derived, melanophore-stimulating peptides. Transfer of the animal to a black background stimulates the production of these peptides and causes a dramatic incr...

journal_title：Journal of cell science

authors： Holthuis JC,Jansen EJ,van Riel MC,Martens GJ

更新日期：1995-10-01 00:00:00

abstract：:Using a novel PCR approach, we have cloned a cDNA encoding the entire high molecular weight tau molecule from rat dorsal root ganglia. The resulting 2080 bp cDNA differs from low molecular weight rat brain tau by the insertion of a novel 762 bp region (exon 4a) between exons 4 and 5. This cDNA clone is identical in se...

journal_title：Journal of cell science

authors： Georgieff IS,Liem RK,Couchie D,Mavilia C,Nunez J,Shelanski ML

更新日期：1993-07-01 00:00:00

abstract：:Vertebrate tropomyosins (TMs) are expressed from four genes, and at least 18 distinct isoforms are generated via a complex pattern of alternative RNA splicing and alternative promoters. The functional significance of this isoform diversity is largely unknown and it remains to be determined whether specific isoforms ar...

journal_title：Journal of cell science

authors： Helfman DM,Berthier C,Grossman J,Leu M,Ehler E,Perriard E,Perriard JC

更新日期：1999-02-01 00:00:00

abstract：:The development of DNA microarrays to study simultaneously the level of mRNA expressed from thousands of genes offers great promise to cell biologists. Microarrays can be used to gain detailed information about transcriptional changes involved in a specific pathway, potentially leading to the identification of novel c...

journal_title：Journal of cell science

authors： Schulze A,Downward J

更新日期：2000-12-01 00:00:00

abstract：:An important aspect of wound healing is the recruitment of neutrophils to the site of infection or tissue injury. Lumican, an extracellular matrix component belonging to the small leucine rich proteoglycan (SLRP) family, is one of the major keratan sulfate proteoglycans (KSPGs) within the corneal stroma. Increasing ev...

journal_title：Journal of cell science

authors： Hayashi Y,Call MK,Chikama T,Liu H,Carlson EC,Sun Y,Pearlman E,Funderburgh JL,Babcock G,Liu CY,Ohashi Y,Kao WW

更新日期：2010-09-01 00:00:00

abstract：:Fluorescence illumination can cause phototoxicity that negatively affects living samples. This study demonstrates that much of the phototoxicity and photobleaching experienced with live-cell fluorescence imaging occurs as a result of 'illumination overhead' (IO). This occurs when a sample is illuminated but fluorescen...

journal_title：Journal of cell science

authors： Kiepas A,Voorand E,Mubaid F,Siegel PM,Brown CM

更新日期：2020-02-21 00:00:00

abstract：:More than 15 subunits of the integrin family of cell surface adhesion molecules have been identified. The alpha 6 beta 4 integrin has recently been identified as a component of hemidesmosomes of stratified squamous epithelium. The monoclonal antibody (mAb) 346-11A binds to the beta 4 subunit in mice. Sequence analysis...

journal_title：Journal of cell science

authors： Kennel SJ,Godfrey V,Ch'ang LY,Lankford TK,Foote LJ,Makkinje A

更新日期：1992-01-01 00:00:00

abstract：:Syndecan-4 is a ubiquitously expressed transmembrane heparan sulphate proteoglycan acting in concert with integrins in the formation of focal adhesions and stress fibres. Signalling events studied thus far suggest the formation of a ternary complex between syndecan-4, phosphatidylinositol 4,5-bisphosphate and protein ...

journal_title：Journal of cell science

authors： Dovas A,Yoneda A,Couchman JR

更新日期：2006-07-01 00:00:00

abstract：:The subcellular localization of human acidic FGF (aFGF; FGF-1) expressed to high levels by using a bacteriophage T7 RNA polymerase-driven vaccinia virus expression system was studied in BHK21 and HeLa cells. Acidic FGF was detected by immunoblotting or immunofluorescence using an affinity-purified rabbit polyclonal an...

journal_title：Journal of cell science

authors： Cao Y,Ekström M,Pettersson RF

更新日期：1993-01-01 00:00:00

abstract：:It remains unclear how GPI-anchored proteins (GPIAPs), which lack cytoplasmic domains, transduce signals triggered by specific ligation. Such signal transduction has been speculated to require the ligated GPIAP to associate with membrane-spanning proteins that communicate with obligate cytoplasmic proteins. Transient ...

journal_title：Journal of cell science

authors： Chen Y,Veracini L,Benistant C,Jacobson K

更新日期：2009-11-01 00:00:00

abstract：:The conversion of the cellular prion protein (PrPc) into a pathogenic isoform (PrP(Sc)) is one of the underlying events in the pathogenesis of the fatal transmissible spongiform encephalopathies (TSEs). Numerous compounds have been described to inhibit prion replication and PrP(Sc) accumulation in cell culture. Among ...

journal_title：Journal of cell science

authors： Nunziante M,Kehler C,Maas E,Kassack MU,Groschup M,Schätzl HM

更新日期：2005-11-01 00:00:00

abstract：:The COP9 signalosome (CSN) is an evolutionarily conserved macromolecular complex that interacts with cullin-RING E3 ligases (CRLs) and regulates their activity by hydrolyzing cullin-Nedd8 conjugates. The CSN sequesters inactive CRL4(Ddb2), which rapidly dissociates from the CSN upon DNA damage. Here we systematically ...

journal_title：Journal of cell science

authors： Olma MH,Roy M,Le Bihan T,Sumara I,Maerki S,Larsen B,Quadroni M,Peter M,Tyers M,Pintard L

更新日期：2009-04-01 00:00:00

abstract：:Mutations in the maternal-effect gene abnormal chromatin (abc) in Drosophila melanogaster result in a variety of defects involving nuclear replication/division. Three recessive alleles of this gene, which maps near 51F on chromosome 2, all result in female sterility. They cause slower embryonic development that is usu...

journal_title：Journal of cell science

authors： Vessey KB,Ludwiczak RL,Briot AS,Underwood EM

更新日期：1991-02-01 00:00:00

abstract：:The monoclonal antibody Bx63 recognizes a centrosomal antigen of Drosophila melanogaster by indirect immunofluorescence and identifies two proteins, with apparent molecular weights of 185 x 10(3) and 66 x 10(3), on Western blots. We have used this antibody to isolate five clones (lambda cs1, -2, -3, -4 and lambda j63)...

journal_title：Journal of cell science

authors： Whitfield WG,Millar SE,Saumweber H,Frasch M,Glover DM

更新日期：1988-04-01 00:00:00

abstract：:Accumulation of adipocytes and collagen type-I-producing cells (fibrosis) is observed in muscular dystrophies. The origin of these cells had been largely unknown, but recently we identified mesenchymal progenitors positive for platelet-derived growth factor receptor alpha (PDGFRα) as the origin of adipocytes in skelet...

journal_title：Journal of cell science

authors： Uezumi A,Ito T,Morikawa D,Shimizu N,Yoneda T,Segawa M,Yamaguchi M,Ogawa R,Matev MM,Miyagoe-Suzuki Y,Takeda S,Tsujikawa K,Tsuchida K,Yamamoto H,Fukada S

更新日期：2011-11-01 00:00:00

abstract：:Programmed cell death comprises several subtypes, as revealed by electron microscopy. Apoptosis or type I programmed cell death is characterized by condensation of cytoplasm and preservation of organelles, essentially without autophagic degradation. Autophagic cell death or type II programmed cell death exhibits exten...

journal_title：Journal of cell science

authors： Bursch W,Hochegger K,Torok L,Marian B,Ellinger A,Hermann RS

更新日期：2000-04-01 00:00:00

abstract：:During directed cell migration, the movement of the nucleus is coupled to the forward progression of the cell. The microtubule motor cytoplasmic dynein is required for both cell polarization and cell motility. Here, we investigate the mechanism by which dynein contributes to directed migration. Knockdown of dynein slo...

journal_title：Journal of cell science

authors： Levy JR,Holzbaur EL

更新日期：2008-10-01 00:00:00

abstract：:Quantitative experimental data from studies of intracellular processing and transport of secretory and membrane proteins are only rarely evaluated using kinetic modelling. Instead, the analysis of such data is usually limited to calculating either the apparent half-life or the transit time. Neither of these parameters...

journal_title：Journal of cell science

authors： Noe DA,Delenick JC

更新日期：1989-03-01 00:00:00

abstract：:Antithrombin inhibits chemokine-induced migration of neutrophils by activating heparan sulfate proteoglycan-dependent signaling. Whether antithrombin affects migration of other types of leukocytes is not known. We investigated the effects of antithrombin on spontaneous and chemokine-triggered migration of lymphocytes ...

journal_title：Journal of cell science

authors： Kaneider NC,Reinisch CM,Dunzendorfer S,Römisch J,Wiedermann CJ

更新日期：2002-01-01 00:00:00

abstract：:HEK293 cells expressing wild-type CXCR2 recruit PH-Akt-GFP to the leading edge of the cell in response to chemokine. However, in cells expressing mutant CXCR2 defective in AP-2 and HIP binding, i.e. with a mutation in the LLKIL motif, PH-Akt-GFP does not localize to the leading edge in response to ligand. Inhibition o...

journal_title：Journal of cell science

authors： Sai J,Fan GH,Wang D,Richmond A

更新日期：2004-11-01 00:00:00

abstract：:Rho GTPases and their downstream effectors regulate changes in the actin cytoskeleton that underlie cell motility and adhesion. They also participate, with RhoA, in the regulation of gene transcription by activating serum response factor (SRF)-mediated transcription from the serum response element (SRE). SRF-mediated ...

journal_title：Journal of cell science

authors： Xie Y,Tan EJ,Wee S,Manser E,Lim L,Koh CG

更新日期：2008-02-15 00:00:00

abstract：:AMP-activated protein kinase (AMPK), a cellular metabolic sensor, is essential in energy regulation and metabolism. Hepatocyte polarization during liver development and regeneration parallels increased metabolism. The current study investigates the effects of AMPK and its upstream activator LKB1 on polarity and bile c...

journal_title：Journal of cell science

authors： Fu D,Wakabayashi Y,Ido Y,Lippincott-Schwartz J,Arias IM

更新日期：2010-10-01 00:00:00

abstract：:Eukaryotic plasma membranes assemble actin filaments within seconds of activation of many receptors, especially during chemotaxis. Here, serum or sphingosine-1-phosphate stimulation of J774 and RAW macrophages released ADP within seconds into the extracellular medium, along with an adenylate kinase activity that conve...

journal_title：Journal of cell science

authors： Kuehnel MP,Reiss M,Anand PK,Treede I,Holzer D,Hoffmann E,Klapperstueck M,Steinberg TH,Markwardt F,Griffiths G

更新日期：2009-02-15 00:00:00

abstract：:How ion channels localize and distribute on the cell membrane remains incompletely understood. We show that interventions that vary cell adhesion proteins and cell size also affect the membrane current density of inward-rectifier K+ channels (Kir2.1; encoded by KCNJ2) and profoundly alter the action potential shape of...

journal_title：Journal of cell science

authors： Sengupta S,Rothenberg KE,Li H,Hoffman BD,Bursac N

更新日期：2019-09-02 00:00:00

abstract：:Endocytic trafficking is regulated by ubiquitylation (also known as ubiquitination) of cargoes and endocytic machineries. The role of ubiquitylation in lysosomal delivery has been well documented, but its role in the recycling pathway is largely unknown. Here, we report that the ubiquitin (Ub) ligase RFFL regulates ub...

journal_title：Journal of cell science

authors： Sakai R,Fukuda R,Unida S,Aki M,Ono Y,Endo A,Kusumi S,Koga D,Fukushima T,Komada M,Okiyoneda T

更新日期：2019-02-07 00:00:00

abstract：:Proper cellular functioning requires that cellular machinery behave in a spatiotemporally regulated manner in response to global changes in nutrient availability. Mounting evidence suggests that one way this is achieved is through the establishment of physically defined gradients of O-GlcNAcylation (O-linked addition ...

journal_title：Journal of cell science

authors： Harwood KR,Hanover JA

更新日期：2014-05-01 00:00:00

abstract：:Cell migration is a complex process requiring density and rigidity sensing of the microenvironment to adapt cell migratory speed through focal adhesion and actin cytoskeleton regulation. ICAP-1 (also known as ITGB1BP1), a β1 integrin partner, is essential for ensuring integrin activation cycle and focal adhesion forma...

journal_title：Journal of cell science

authors： Bouin AP,Kyumurkov A,Régent-Kloeckner M,Ribba AS,Faurobert E,Fournier HN,Bourrin-Reynard I,Manet-Dupé S,Oddou C,Balland M,Planus E,Albiges-Rizo C

更新日期：2017-02-01 00:00:00

abstract：:Hormone secretion by pituitary cells is decreased by roscovitine, an inhibitor of cyclin-dependent kinase 5 (Cdk5). Roscovitine treatment reorganizes cortical actin and ultrastructural analysis demonstrates that roscovitine limits the ability of secretory granules to approach the plasma membrane or one another. Trio, ...

journal_title：Journal of cell science

authors： Xin X,Ferraro F,Bäck N,Eipper BA,Mains RE

更新日期：2004-09-15 00:00:00

abstract：:Epithelial junctions depend on intercellular interactions between β(1) subunits of the Na(+)/K(+)-ATPase molecules of neighboring cells. The interaction between dog and rat subunits is less effective than the interaction between two dog β(1) subunits, indicating the importance of species-specific regions for β(1)-β(1)...

journal_title：Journal of cell science

authors： Tokhtaeva E,Sachs G,Sun H,Dada LA,Sznajder JI,Vagin O

更新日期：2012-03-15 00:00:00

abstract：:In many epithelial cells, epidermal growth factor (EGF) augments the epithelial-mesenchymal transition (EMT) that occurs when cells are treated with transforming growth factor β (TGFβ). We demonstrate that this augmentation requires activation of SH2 domain-containing phosphatase-2 (SHP2; also known as PTPN11), a prot...

journal_title：Journal of cell science

authors： Buonato JM,Lan IS,Lazzara MJ

更新日期：2015-11-01 00:00:00