Abstract:
:Using a novel PCR approach, we have cloned a cDNA encoding the entire high molecular weight tau molecule from rat dorsal root ganglia. The resulting 2080 bp cDNA differs from low molecular weight rat brain tau by the insertion of a novel 762 bp region (exon 4a) between exons 4 and 5. This cDNA clone is identical in sequence with a high molecular weight tau (HMW) cDNA from rat PC12 tumor cells and is closely related to a HMW tau cDNA from mouse N115 tumor cells. In vitro transcription/translation produces a protein that migrates on SDS-PAGE with the same apparent molecular weight as HMW tau purified from rat sciatic nerve. The HMW tau protein is generated from an 8 kb mRNA, which can be detected by northern blots in peripheral ganglia, but not in brain. A more sensitive assay using PCR and Southern blot analysis demonstrates the presence of exon 4a in spinal cord and in retina. In combination with immunohistochemical studies of spinal cord, these data suggest that HMW tau, though primarily in the peripheral nervous system, is also expressed in limited areas of the central nervous system, although its presence cannot be detected in the cerebral cortices.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Georgieff IS,Liem RK,Couchie D,Mavilia C,Nunez J,Shelanski MLsubject
Has Abstractpub_date
1993-07-01 00:00:00pages
729-37eissn
0021-9533issn
1477-9137journal_volume
105 ( Pt 3)pub_type
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2010-12-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2002-05-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2012-11-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2017-11-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:
更新日期:2000-08-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-11-01 00:00:00
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journal_title:Journal of cell science
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doi:
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1995-02-01 00:00:00
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journal_title:Journal of cell science
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更新日期:1995-05-01 00:00:00
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journal_title:Journal of cell science
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