CAF-1 and the inheritance of chromatin states: at the crossroads of DNA replication and repair.

abstract：:Macroautophagy is morphologically characterized by autophagosome formation. Autophagosomes are double-membraned vesicles that sequester cytoplasmic components for further degradation in the lysosome. Basal autophagy is paramount for intracellular quality control in post-mitotic cells but, surprisingly, the number of a...

journal_title：Journal of cell science

authors： Fernandez-Fernandez MR,Ruiz-Garcia D,Martin-Solana E,Chichon FJ,Carrascosa JL,Fernandez JJ

更新日期：2017-01-01 00:00:00

abstract：:Freeze-fracture cytochemistry with digitonin has been used to examine the cholesterol content of the plasma membrane of uterine epithelial cells during the early stages of pregnancy in the rat. Lesions caused by digitonin complexing with cholesterol were seen on both lateral and apical portions of the membrane but tig...

journal_title：Journal of cell science

authors： Murphy CR,Martin B

更新日期：1985-10-01 00:00:00

abstract：:The plasma membrane Ca2+-ATPase-2 (PMCA2) is expressed in stereocilia of hair cells of the inner ear, whereas PMCA1 is expressed in the basolateral plasma membrane of hair cells. Both extrude excess Ca2+ from the cytosol. They are predicted to contain ten membrane-spanning segments, two large cytoplasmic loops as well...

journal_title：Journal of cell science

authors： Grati M,Aggarwal N,Strehler EE,Wenthold RJ

更新日期：2006-07-15 00:00:00

abstract：:Although the 11.5 kDa Zn(2+)-binding protein (ZnBP, parathymosin-alpha) possesses a functional bipartite nuclear localization signal it was found in most tissues in the cytoplasm. The cultivation of freshly isolated rat hepatocytes for 24 hours under standard conditions was associated with an almost complete transloca...

journal_title：Journal of cell science

authors： Trompeter HI,Schiermeyer A,Blankenburg G,Hennig E,Söling HD

更新日期：1999-11-01 00:00:00

abstract：:Mutation of the LMNA gene, encoding nuclear lamin A and lamin C (hereafter lamin A/C), is a common cause of familial dilated cardiomyopathy (DCM). Among Finnish DCM patients, the founder mutation c.427T>C (p.S143P) is the most frequently reported genetic variant. Here, we show that p.S143P lamin A/C is more nucleoplas...

journal_title：Journal of cell science

authors： West G,Gullmets J,Virtanen L,Li SP,Keinänen A,Shimi T,Mauermann M,Heliö T,Kaartinen M,Ollila L,Kuusisto J,Eriksson JE,Goldman RD,Herrmann H,Taimen P

更新日期：2016-07-15 00:00:00

abstract：:The interplay between pathogen-encoded virulence factors and host cell signaling networks is critical for both the establishment and clearance of microbial infections. Yersinia uptake into host cells serves as an in vitro model for exploring how host cells respond to Yersinia adherence. In this study, we provide insig...

journal_title：Journal of cell science

authors： Bruce-Staskal PJ,Weidow CL,Gibson JJ,Bouton AH

更新日期：2002-07-01 00:00:00

abstract：:CDC6, a replication licensing protein, is partially exported to the cytoplasm in human cells through phosphorylation by Cdk during S phase, but a significant proportion remains in the nucleus. We report here that human CDC6 physically interacts with ATR, a crucial checkpoint kinase, in a manner that is stimulated by p...

journal_title：Journal of cell science

authors： Yoshida K,Sugimoto N,Iwahori S,Yugawa T,Narisawa-Saito M,Kiyono T,Fujita M

更新日期：2010-01-15 00:00:00

abstract：:Errors in mitotic chromosome segregation can lead to DNA damage and aneuploidy, both hallmarks of cancer. To achieve synchronous error-free segregation, mitotic chromosomes must align at the metaphase plate with stable amphitelic attachments to microtubules emanating from opposing spindle poles. The astrin-kinastrin (...

journal_title：Journal of cell science

authors： Geraghty Z,Barnard C,Uluocak P,Gruneberg U

更新日期：2021-01-08 00:00:00

abstract：:MIP and MP70 are putative gap junction components in the plasma membranes of the mammalian lens fibre cells. We show now that MP70 can be solubilized separately from MIP in mild detergent solutions, and that this treatment results in the dissociation of the fibre gap junctions. Solubilized MP70 was isolated as 16.9 S ...

journal_title：Journal of cell science

authors： Kistler J,Bullivant S

更新日期：1988-11-01 00:00:00

abstract：:PML nuclear bodies (NBs) are involved in the regulation of key nuclear pathways but their biochemical function in nuclear metabolism is unknown. In this study PML NB assembly dynamics were assessed by live cell imaging and mathematic modeling of its major component parts. We show that all six nuclear PML isoforms exhi...

journal_title：Journal of cell science

authors： Weidtkamp-Peters S,Lenser T,Negorev D,Gerstner N,Hofmann TG,Schwanitz G,Hoischen C,Maul G,Dittrich P,Hemmerich P

更新日期：2008-08-15 00:00:00

abstract：:Apical and basolateral endocytic pathways in polarised Caco-2 cells were investigated by following the uptake, recycling and transcytosis of the galactose-binding protein toxin ricin, as a membrane marker. Differences in the extent and kinetics of lectin uptake, recycling and transcytosis were observed at the apical a...

journal_title：Journal of cell science

authors： Jackman MR,Shurety W,Ellis JA,Luzio JP

更新日期：1994-09-01 00:00:00

abstract：:Mutations in inositol polyphosphate 5-phosphatase E (INPP5E) cause the ciliopathies known as Joubert and MORM syndromes; however, the role of INPP5E in ciliary biology is not well understood. Here, we describe an interaction between INPP5E and AURKA, a centrosomal kinase that regulates mitosis and ciliary disassembly,...

journal_title：Journal of cell science

authors： Plotnikova OV,Seo S,Cottle DL,Conduit S,Hakim S,Dyson JM,Mitchell CA,Smyth IM

更新日期：2015-01-15 00:00:00

abstract：:Activator of G-protein signaling 3 (AGS3, also known as GPSM1) regulates the trans-Golgi network. The AGS3 GoLoco motif binds to Gαi and thereby regulates the transport of proteins to the plasma membrane. Compaction of early embryos is based on the accumulation of E-cadherin (Cdh1) at cell-contacted membranes. However...

journal_title：Journal of cell science

authors： Nie ZW,Niu YJ,Zhou W,Zhou DJ,Kim JY,Cui XS

更新日期：2020-12-07 00:00:00

abstract：:The neuromuscular junction (NMJ) is the largest, most-complex synapse in the human body. Motor neuron (MN) diseases, such as amyotrophic lateral sclerosis (ALS), specifically target MNs and the NMJs. However, little is known about the reasons for MN-selective neuronal and synaptic vulnerability in MN diseases. Here, u...

journal_title：Journal of cell science

authors： Altman T,Geller D,Kleeblatt E,Gradus-Perry T,Perlson E

更新日期：2019-12-02 00:00:00

abstract：:The cell wall of 24-h zygotes of Fucus serratus is composed of 3 layers--an inner fibrillar layer (sulphated fucan), an outer fibrillar layer (alginic aicd/cellulose) and an exterior amorphous layer (sulphated fucan, alginic acid). The 2 layers containing sulphated fucan are preferentially thickened at the rhizoid pol...

journal_title：Journal of cell science

authors： Callow ME,Coughlan SJ,Evans LV

更新日期：1978-08-01 00:00:00

abstract：:Centriolar satellites are membrane-less structures that localize and move around the centrosome and cilium complex in a microtubule-dependent manner. They play important roles in centrosome- and cilium-related processes, including protein trafficking to the centrosome and cilium complex, and ciliogenesis, and they are...

journal_title：Journal of cell science

authors： Conkar D,Culfa E,Odabasi E,Rauniyar N,Yates JR 3rd,Firat-Karalar EN

更新日期：2017-04-15 00:00:00

abstract：:Organelle transport is vital for the development and maintenance of axons, in which the distances between sites of organelle biogenesis, function, and recycling or degradation can be vast. Movement of mitochondria in axons can serve as a general model for how all organelles move: mitochondria are easy to identify, the...

journal_title：Journal of cell science

authors： Hollenbeck PJ,Saxton WM

更新日期：2005-12-01 00:00:00

abstract：:Primary cilia are conserved organelles that play crucial roles as mechano- and chemosensors, as well as transducing signaling cascades. Consequently, ciliary dysfunction results in a broad range of phenotypes: the ciliopathies. Bardet-Biedl syndrome (BBS), a model ciliopathy, is caused by mutations in 16 known genes. ...

journal_title：Journal of cell science

authors： Gascue C,Tan PL,Cardenas-Rodriguez M,Libisch G,Fernandez-Calero T,Liu YP,Astrada S,Robello C,Naya H,Katsanis N,Badano JL

更新日期：2012-01-15 00:00:00

abstract：:Podosomes, adhesion structures capable of matrix degradation, have been linked with the ability of cells to perform chemotaxis and invade tissues. Wiskott-Aldrich Syndrome protein (WASp), an effector of the RhoGTPase Cdc42 and a Src family kinase substrate, regulates macrophage podosome formation. In this study, we de...

journal_title：Journal of cell science

authors： Dovas A,Gevrey JC,Grossi A,Park H,Abou-Kheir W,Cox D

更新日期：2009-11-01 00:00:00

abstract：:A Mr 60,000 protein of the axonal cortical cytoplasm, which is recognized by a novel monoclonal antibody, is described. The antibody, DR1, was produced by immunizing mice with a soluble extract of bovine brain membranes that is enriched in known membrane cytoskeletal proteins. DR1 recognizes a Mr 60,000 protein in thi...

journal_title：Journal of cell science

authors： Rayner DA,Baines AJ

更新日期：1989-11-01 00:00:00

abstract：:Mutations in leucine-rich repeat kinase 2 (LRRK2) are associated with Parkinson’s disease, but the precise physiological function of the protein remains ill-defined. Recently, our group proposed a model in which LRRK2 kinase activity is part of an EndoA phosphorylation cycle that facilitates efficient vesicle formatio...

journal_title：Journal of cell science

authors： Arranz AM,Delbroek L,Van Kolen K,Guimarães MR,Mandemakers W,Daneels G,Matta S,Calafate S,Shaban H,Baatsen P,De Bock PJ,Gevaert K,Vanden Berghe P,Verstreken P,De Strooper B,Moechars D

更新日期：2015-02-01 00:00:00

abstract：:HEK293 cells expressing wild-type CXCR2 recruit PH-Akt-GFP to the leading edge of the cell in response to chemokine. However, in cells expressing mutant CXCR2 defective in AP-2 and HIP binding, i.e. with a mutation in the LLKIL motif, PH-Akt-GFP does not localize to the leading edge in response to ligand. Inhibition o...

journal_title：Journal of cell science

authors： Sai J,Fan GH,Wang D,Richmond A

更新日期：2004-11-01 00:00:00

abstract：:After DNA replication, cells condense their chromosomes in order to segregate them during mitosis. The condensation process as well as subsequent segregation requires phosphorylation of histone H3 at serine 10. Histone H3 phosphorylation initiates during G2 in pericentric foci prior to H3 phosphorylation in the chromo...

journal_title：Journal of cell science

authors： Zeitlin SG,Barber CM,Allis CD,Sullivan KF

更新日期：2001-02-01 00:00:00

abstract：:Using a novel PCR approach, we have cloned a cDNA encoding the entire high molecular weight tau molecule from rat dorsal root ganglia. The resulting 2080 bp cDNA differs from low molecular weight rat brain tau by the insertion of a novel 762 bp region (exon 4a) between exons 4 and 5. This cDNA clone is identical in se...

journal_title：Journal of cell science

authors： Georgieff IS,Liem RK,Couchie D,Mavilia C,Nunez J,Shelanski ML

更新日期：1993-07-01 00:00:00

abstract：:Nuclear lamin A and C alleles that are linked to three distinct human diseases have been expressed both in HeLa cells and in fibroblasts derived from Lmna null mice. Point mutations that cause dilated cardiomyopathy (L85R and N195K) and autosomal dominant Emery-Dreifuss muscular dystrophy (L530P) modify the assembly p...

journal_title：Journal of cell science

authors： Raharjo WH,Enarson P,Sullivan T,Stewart CL,Burke B

更新日期：2001-12-01 00:00:00

abstract：:Insulin-like growth factor-1 (IGF-1) has been shown to induce skeletal muscle hypertrophy, to prevent the loss of muscle mass with ageing and to improve the muscle phenotype of dystrophic mice. We previously developed a model of IGF-1-induced hypertrophy of human myotubes, in which hypertrophy was not only characteriz...

journal_title：Journal of cell science

authors： Jacquemin V,Butler-Browne GS,Furling D,Mouly V

更新日期：2007-02-15 00:00:00

abstract：:The protein kinase inhibitor H-7 prevents the assembly of tight junctions in cultured Madin Darby Canine Kidney (MDCK) epithelial cells (Balda et al. (1991) J. Membr. Biol. 122, 193-202; Nigam et al. (1991) Biochem. Biophys. Res. Commun. 181, 548-553); however, its mechanism of action is unknown. To understand the bas...

journal_title：Journal of cell science

authors： Denisenko N,Burighel P,Citi S

更新日期：1994-04-01 00:00:00

abstract：:Endothelial barrier dysfunction leads to edema and vascular leak, causing high morbidity and mortality. Previously, Abl kinase inhibition has been shown to protect against vascular leak. Using the distinct inhibitory profiles of clinically available Abl kinase inhibitors, we aimed to provide a mechanistic basis for no...

journal_title：Journal of cell science

authors： Botros L,Pronk MCA,Juschten J,Liddle J,Morsing SKH,van Buul JD,Bates RH,Tuinman PR,van Bezu JSM,Huveneers S,Bogaard HJ,van Hinsbergh VWM,Hordijk PL,Aman J

更新日期：2020-05-14 00:00:00

abstract：:In primary cultures of rat cerebellar granule cells with a functional network of glutamatergic neurons, the expression pattern of the different subunits of nitric-oxide (NO)-sensitive guanylyl cyclase changes during cell differentiation. These cells express the alpha1, alpha2 and beta1 subunits of NO-sensitive guanyly...

journal_title：Journal of cell science

authors： Jurado S,Sanchez-Prieto J,Torres M

更新日期：2003-08-01 00:00:00

abstract：:Polarization of early embryos provides a foundation to execute essential patterning and morphogenetic events. In Caenorhabditis elegans, cell contacts polarize early embryos along their radial axis by excluding the cortical polarity protein PAR-6 from sites of cell contact, thereby restricting PAR-6 to contact-free ce...

journal_title：Journal of cell science

authors： Chan E,Nance J

更新日期：2013-04-01 00:00:00