当前位置： SCI文献检索 > JOURNAL OF CELL SCIENCE期刊下所有文献 > Regulation of podosome dynamics by WASp phosphorylation: implication in matrix degradation and chemotaxis in macrophages.

Regulation of podosome dynamics by WASp phosphorylation: implication in matrix degradation and chemotaxis in macrophages.

Abstract：

:Podosomes, adhesion structures capable of matrix degradation, have been linked with the ability of cells to perform chemotaxis and invade tissues. Wiskott-Aldrich Syndrome protein (WASp), an effector of the RhoGTPase Cdc42 and a Src family kinase substrate, regulates macrophage podosome formation. In this study, we demonstrate that WASp is active in podosomes by using TIRF-FRET microscopy. Pharmacological and RNA interference approaches suggested that continuous WASp activity is required for podosome formation and function. Rescue experiments using point mutations demonstrate an absolute requirement for Cdc42 binding to WASp in podosome formation. Although tyrosine phosphorylation was not absolutely required for podosome formation, phosphorylation did regulate the rate of podosome nucleation and actin filament stability. Importantly, WASp tyrosine phosphorylation does not alter WASp activation, instead phosphorylation appears to be important for the restriction of WASp activity to podosomes. In addition, the matrix-degrading ability of cells requires WASp phosphorylation. Chemotactic responses to CSF-1 were also attenuated in the absence of endogenous WASp, which could not be rescued with either tyrosine mutation. These results suggest a more complex role for tyrosine phosphorylation than simply in the regulation of WASp activity, and suggest a link between podosome dynamics and macrophage migration.

journal_name

J Cell Sci

journal_title

Journal of cell science

authors

Dovas A,Gevrey JC,Grossi A,Park H,Abou-Kheir W,Cox D

doi

10.1242/jcs.051755

subject

Has Abstract

pub_date

2009-11-01 00:00:00

pages

3873-82

issue

Pt 21

eissn

0021-9533

issn

1477-9137

pii

jcs.051755

journal_volume

122

pub_type

杂志文章

相关文献

JOURNAL OF CELL SCIENCE文献大全
  • BCAP is a centriolar satellite protein and inhibitor of ciliogenesis.

    abstract：:The centrosome and cilium are organelles with important roles in microtubule organisation, cell division, cell signalling, embryogenesis and tissue homeostasis. The two organelles are mutually exclusive. The centriole/basal body is found at the core of the centrosome (centriole) or at the base of the cilium (basal bod...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.196642

    authors： de Saram P,Iqbal A,Murdoch JN,Wilkinson CJ

    更新日期：2017-10-01 00:00:00

  • The endo-beta-1,3-glucanase eng1p is required for dissolution of the primary septum during cell separation in Schizosaccharomyces pombe.

    abstract：:Schizosaccharomyces pombe cells divide by medial fission throughout contraction of an actomyosin ring and deposition of a multilayered division septum that must be cleaved to release the two daughter cells. Although many studies have focused on the actomoysin ring and septum assembly, little information is available c...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.00377

    authors： Martín-Cuadrado AB,Dueñas E,Sipiczki M,Vázquez de Aldana CR,del Rey F

    更新日期：2003-05-01 00:00:00

  • Ugo1 and Mdm30 act sequentially during Fzo1-mediated mitochondrial outer membrane fusion.

    abstract：:Dynamin-related GTPase proteins (DRPs) are main players in membrane remodelling. Conserved DRPs called mitofusins (Mfn1/Mfn2/Fzo1) mediate the fusion of mitochondrial outer membranes (OM). OM fusion depends on self-assembly and GTPase activity of mitofusins as well as on two other proteins, Ugo1 and Mdm30. Here, we de...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.073080

    authors： Anton F,Fres JM,Schauss A,Pinson B,Praefcke GJ,Langer T,Escobar-Henriques M

    更新日期：2011-04-01 00:00:00

  • Studies of membrane fusion. IV. Fusion of HeLa cells with Sendai virus.

    abstract：:The Sendai virus-induced fusion of HeLa cells has been studied by freeze-fracture electron microscopy. Freeze-fracture observations confirm previous scanning electron-microscope studies (1977) and show that at 4 degrees C virus particles bind to the cell surface and that cell agglutination results from the crosslinkin...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Knutton S

    更新日期：1979-04-01 00:00:00

  • A novel role for E- and P-selectins: shape control of endothelial cell monolayers.

    abstract：:The migration of neutrophils from blood vessels to peripheral tissues is a key step of inflammation. This requires the formation of transient gaps between endothelial cells with concomitant leucocyte squeezing through these narrow apertures and immediate restoration of endothelium continuity. It is currently considere...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Kaplanski G,Farnarier C,Benoliel AM,Foa C,Kaplanski S,Bongrand P

    更新日期：1994-09-01 00:00:00

  • The calpain small subunit regulates cell-substrate mechanical interactions during fibroblast migration.

    abstract：:Cell migration involves the dynamic formation and release of cell-substrate adhesions, where the exertion and detection of mechanical forces take place. Members of the calpain family of calcium-dependent proteases are believed to have a central role in these processes, possibly through the regulation of focal adhesion...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.036152

    authors： Undyala VV,Dembo M,Cembrola K,Perrin BJ,Huttenlocher A,Elce JS,Greer PA,Wang YL,Beningo KA

    更新日期：2008-11-01 00:00:00

  • Vam3p, a new member of syntaxin related protein, is required for vacuolar assembly in the yeast Saccharomyces cerevisiae.

    abstract：:Syntaxins are thought to participate in the specific interactions between vesicles and acceptor membranes in intracellular protein trafficking. VAM3 of Saccharomyces cerevisiae encodes a 33 kDa protein (Vam3p) with a hydrophobic transmembrane segment at its C terminus. Vam3p has structural similarities to syntaxins of...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Wada Y,Nakamura N,Ohsumi Y,Hirata A

    更新日期：1997-06-01 00:00:00

  • Distinct mechanisms of nuclear accumulation regulate the functional consequence of E2F transcription factors.

    abstract：:Transcription factor E2F plays an important role in coordinating and integrating early cell cycle progression with the transcription apparatus. It is known that physiological E2F arises when a member of two families of proteins, E2F and DP, interact as E2F/DP heterodimers and that transcriptional activity is regulated...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Allen KE,de la Luna S,Kerkhoven RM,Bernards R,La Thangue NB

    更新日期：1997-11-01 00:00:00

  • Identification of MYO4, a second class V myosin gene in yeast.

    abstract：:We have isolated a fourth myosin gene (MYO4) in yeast (Saccharomyces cerevisiae). MYO4 encodes a approximately 170 kDa (1471 amino acid) class V myosin, using the classification devised by Cheney et al. (1993a; Cell Motil. Cytoskel. 24, 215-223); the motor domain is followed by a neck region containing six putative ca...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Haarer BK,Petzold A,Lillie SH,Brown SS

    更新日期：1994-04-01 00:00:00

  • An analysis of Con A-mediated agglutination in a Chinese hamster ovary subclone which responds morphologically to growth in dibutyryl cyclic AMP. III. The role of microvilli in the agglutination process.

    abstract：:We have used the H-7w subclone of a Chinese hamster ovary cell line (K1) to investigate the role of cell surface architecture (specifically microvilli, blebs, and sheets) in determining the relative agglutinability of a cell line with Con A. Our evidence clearly demonstrates that no specific, immediately recognizable ...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Noonan KD,Ukena T

    更新日期：1978-12-01 00:00:00

  • Loss of PPARγ in endothelial cells leads to impaired angiogenesis.

    abstract：:Tie2-promoter-mediated loss of peroxisome proliferator-activated receptor gamma (PPARγ, also known as PPARG) in mice leads to osteopetrosis and pulmonary arterial hypertension. Vascular disease is associated with loss of PPARγ in pulmonary microvascular endothelial cells (PMVEC); we evaluated the role of PPARγ in PMVE...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.169011

    authors： Vattulainen-Collanus S,Akinrinade O,Li M,Koskenvuo M,Li CG,Rao SP,de Jesus Perez V,Yuan K,Sawada H,Koskenvuo JW,Alvira C,Rabinovitch M,Alastalo TP

    更新日期：2016-02-15 00:00:00

  • Cyclic cytoplasmic activity of non-nucleate egg fragments of Xenopus controls the morphology of injected sperms.

    abstract：:Triton X-100-treated sperms were injected into non-nucleate egg fragments of Xenopus laevis to determine whether the structure of the injected sperm nucleus is affected by the cyclic activity of the cytoplasm. Swollen vesicular nuclei were very frequently observed when the sperms were injected and incubated during the...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Sakai M,Shinagawa A

    更新日期：1983-09-01 00:00:00

  • Biosynthesis and transport of lysosomal alpha-glucosidase in the human colon carcinoma cell line Caco-2: secretion from the apical surface.

    abstract：:The human adenocarcinoma cell line Caco-2 was used for studies on the biosynthesis and transport of lysosomal acid alpha-glucosidase in polarized epithelial cells. Metabolic labelling revealed that in Caco-2 cells alpha-glucosidase is synthesized as a precursor form of 110 x 10(3) Mr. This form is converted into a pre...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Klumperman J,Fransen JA,Boekestijn TC,Oude Elferink RP,Matter K,Hauri HP,Tager JM,Ginsel LA

    更新日期：1991-10-01 00:00:00

  • Dissociation of LPA-induced cytoskeletal contraction from stress fiber formation by differential localization of RhoA.

    abstract：:Addition of lysophosphatidic acid (LPA) to serum-deprived N1E-115 neuronal cells results in rapid f-actin assembly accompanied by neurite retraction and rounding of the cell body due to contraction of the cortical actin cytoskeleton. LPA action is mimicked by activated RhoA, while it is blocked by dominant-negative Rh...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Kranenburg O,Poland M,Gebbink M,Oomen L,Moolenaar WH

    更新日期：1997-10-01 00:00:00

  • The E3 SUMO ligase Nse2 regulates sumoylation and nuclear-to-cytoplasmic translocation of skNAC-Smyd1 in myogenesis.

    abstract：:Skeletal and heart muscle-specific variant of the α subunit of nascent polypeptide associated complex (skNAC; encoded by NACA) is exclusively found in striated muscle cells. Its function, however, is largely unknown. Previous reports have demonstrated that skNAC binds to m-Bop/Smyd1, a multi-functional protein that re...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.150334

    authors： Berkholz J,Michalick L,Munz B

    更新日期：2014-09-01 00:00:00

  • Epigenetic assembly of centromeric chromatin at ectopic alpha-satellite sites on human chromosomes.

    abstract：:To investigate the mechanism of chromatin assembly at human centromeres, we isolated cultured human cell lines in which a transfected alpha-satellite (alphoid) YAC was integrated ectopically into the terminal region of host chromosome 16, where it was stably maintained. Centromere activity of the alphoid YAC was suppr...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.00697

    authors： Nakano M,Okamoto Y,Ohzeki J,Masumoto H

    更新日期：2003-10-01 00:00:00

  • VRK1 phosphorylates CREB and mediates CCND1 expression.

    abstract：:Vaccinia virus B1 kinase plays a key role in viral DNA replication. The homologous mammalian vaccinia-related kinases (VRKs) are also implicated in the regulation of DNA replication, although direct evidence remains elusive. Here we show that VRK1 regulates cell cycle progression in the DNA replication period by induc...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.026757

    authors： Kang TH,Park DY,Kim W,Kim KT

    更新日期：2008-09-15 00:00:00

  • The development of lampbrush chromosome-type transcription in the early diplotene oocytes of Xenopus laevis: an electron-microscope analysis.

    abstract：:Oocytes of Xenopus laevis in pachytene and early diplotene of meiosis have been studied using the Miller spreading technique. Transcription first appears in germinal vesicles 25-40 micrometers in diameter, when the oocyte is in early diplotene. Transcription at this stage consists of arrays of short RNP transcripts, i...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Hill RS,Macgregor HC

    更新日期：1980-08-01 00:00:00

  • The PIKfyve complex regulates the early melanosome homeostasis required for physiological amyloid formation.

    abstract：:The metabolism of PI(3,5)P2 is regulated by the PIKfyve, VAC14 and FIG4 complex, mutations in which are associated with hypopigmentation in mice. These pigmentation defects indicate a key, but as yet unexplored, physiological relevance of this complex in the biogenesis of melanosomes. Here, we show that PIKfyve activi...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.229500

    authors： Bissig C,Croisé P,Heiligenstein X,Hurbain I,Lenk GM,Kaufman E,Sannerud R,Annaert W,Meisler MH,Weisman LS,Raposo G,van Niel G

    更新日期：2019-02-28 00:00:00

  • Electrical control of flagellar activity in impaled bull spermatozoa.

    abstract：:The control of bull spermatozoon flagellar activity has been investigated using direct current injection into the cells through an impaling glass microelectrode. Negative current injection results in a decrease in the flagellar frequency. Flagellar frequencies can be decreased to zero with high negative currents. This...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： O'Day PM,Rikmenspoel R

    更新日期：1979-02-01 00:00:00

  • Microtubule glycylation promotes attachment of basal bodies to the cell cortex.

    abstract：:Motile cilia generate directed hydrodynamic flow that is important for the motility of cells and extracellular fluids. To optimize directed hydrodynamic flow, motile cilia are organized and oriented into a polarized array. Basal bodies (BBs) nucleate and position motile cilia at the cell cortex. Cytoplasmic BB-associa...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.233726

    authors： Junker AD,Soh AWJ,O'Toole ET,Meehl JB,Guha M,Winey M,Honts JE,Gaertig J,Pearson CG

    更新日期：2019-08-07 00:00:00

  • Integrating one-dimensional and three-dimensional maps of genomes.

    abstract：:Genomes exist in vivo as complex physical structures, and their functional output (i.e. the gene expression profile of a cell) is related to their spatial organization inside the nucleus as well as to local chromatin status. Chromatin modifications and chromosome conformation are distinct in different tissues and cell...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.051631

    authors： Naumova N,Dekker J

    更新日期：2010-06-15 00:00:00

  • Chromosome associations in budding yeast caused by integrated tandemly repeated transgenes.

    abstract：:The binding of GFP-tagged tetracycline repressor (TetR) molecules to chromosomally integrated tetracycline operator (tetO) sequence repeats has been used as a system to study chromosome behaviour microscopically in vivo. We found that these integrated transgenes influence the architecture of yeast interphase nuclei, a...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Fuchs J,Lorenz A,Loidl J

    更新日期：2002-03-15 00:00:00

  • Characterization of bone morphogenetic protein-6 signaling pathways in osteoblast differentiation.

    abstract：:Bone morphogenetic protein (BMP)-6 is a member of the transforming growth factor (TGF)-(&bgr;) superfamily, and is most similar to BMP-5, osteogenic protein (OP)-1/BMP-7, and OP-2/BMP-8. In the present study, we characterized the endogenous BMP-6 signaling pathway during osteoblast differentiation. BMP-6 strongly indu...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Ebisawa T,Tada K,Kitajima I,Tojo K,Sampath TK,Kawabata M,Miyazono K,Imamura T

    更新日期：1999-10-01 00:00:00

  • Tensegrity I. Cell structure and hierarchical systems biology.

    abstract：:In 1993, a Commentary in this journal described how a simple mechanical model of cell structure based on tensegrity architecture can help to explain how cell shape, movement and cytoskeletal mechanics are controlled, as well as how cells sense and respond to mechanical forces (J. Cell Sci. 104, 613-627). The cellular ...

    journal_title：Journal of cell science

    pub_type： 杂志文章,评审

    doi：10.1242/jcs.00359

    authors： Ingber DE

    更新日期：2003-04-01 00:00:00

  • Tau phosphorylation in neuronal cell function and dysfunction.

    abstract：:Tau is a group of neuronal microtubule-associated proteins that are formed by alternative mRNA splicing and accumulate in neurofibrillary tangles in Alzheimer's disease (AD) brain. Tau plays a key role in regulating microtubule dynamics, axonal transport and neurite outgrowth, and all these functions of tau are modula...

    journal_title：Journal of cell science

    pub_type： 杂志文章,评审

    doi：10.1242/jcs.01558

    authors： Johnson GV,Stoothoff WH

    更新日期：2004-11-15 00:00:00

  • In situ analyses reveal that the two nuclei of Giardia lamblia are equivalent.

    abstract：:The protozoan parasite Giardia lamblia has the unusual morphology of bearing two equal-sized nuclei. This organism probably represents the earliest diverging lineage of eukaryotes, suggesting that its biological tactics may be transitional. To begin to understand the role played by the two equal-sized nuclei in this o...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Kabnick KS,Peattie DA

    更新日期：1990-03-01 00:00:00

  • SH4-domain-induced plasma membrane dynamization promotes bleb-associated cell motility.

    abstract：:SH4 domains provide bipartite membrane-targeting signals for oncogenic Src family kinases. Here we report the induction of non-apoptotic plasma membrane (PM) blebbing as a novel and conserved activity of SH4 domains derived from the prototypic Src kinases Src, Fyn, Yes and Lck as well as the HASPB protein of Leishmani...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.011130

    authors： Tournaviti S,Hannemann S,Terjung S,Kitzing TM,Stegmayer C,Ritzerfeld J,Walther P,Grosse R,Nickel W,Fackler OT

    更新日期：2007-11-01 00:00:00

  • Rho and Rac but not Cdc42 regulate endothelial cell permeability.

    abstract：:Endothelial permeability induced by thrombin and histamine is accompanied by actin stress fibre assembly and intercellular gap formation. Here, we investigate the roles of the Rho family GTPases Rho1, Rac1 and Cdc42 in regulating endothelial barrier function, and correlate this with their effects on F-actin organizati...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：

    authors： Wójciak-Stothard B,Potempa S,Eichholtz T,Ridley AJ

    更新日期：2001-04-01 00:00:00

  • Modeling the self-organized phosphatidylinositol lipid signaling system in chemotactic cells using quantitative image analysis.

    abstract：:A key signaling event that is responsible for gradient sensing in eukaryotic cell chemotaxis is a phosphatidylinositol (PtdIns) lipid reaction system. The self-organization activity of this PtdIns lipid system induces an inherent polarity, even in the absence of an external chemoattractant gradient, by producing a loc...

    journal_title：Journal of cell science

    pub_type： 杂志文章

    doi：10.1242/jcs.108373

    authors： Shibata T,Nishikawa M,Matsuoka S,Ueda M

    更新日期：2012-11-01 00:00:00