Abstract：
:To investigate the mechanism of chromatin assembly at human centromeres, we isolated cultured human cell lines in which a transfected alpha-satellite (alphoid) YAC was integrated ectopically into the terminal region of host chromosome 16, where it was stably maintained. Centromere activity of the alphoid YAC was suppressed at ectopic locations on the host chromosome, as indicated by the absent or reduced assembly of CENP-A and -C. However, long-term culture in selective medium, or short-term treatment with the histone deacetylase inhibitor Trichostatin A (TSA), promoted the re-assembly of CENPA, -B and -C at the YAC site and the release of minichromosomes containing the YAC integration site. Chromatin immunoprecipitation analyses of the re-formed minichromosome and the alphoid YAC-based stable human artificial chromosome both indicated that CENP-A and CENP-B assembled only on the inserted alphoid array but not on the YAC arms. On the YAC arms at the alphoid YAC integration sites, TSA treatment increased both the acetylation level of histone H3 and the transcriptional level of a marker gene. An increase in the level of transcription was also observed after long-term culture in selective medium. These activities, which are associated with changes in chromatin structure, might reverse the suppressed chromatin state of the YAC at ectopic loci, and thus might be involved in the epigenetic change of silent centromeres on ectopic alphoid loci.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Nakano M,Okamoto Y,Ohzeki J,Masumoto Hdoi
10.1242/jcs.00697keywords:
subject
Has Abstractpub_date
2003-10-01 00:00:00pages
4021-34issue
Pt 19eissn
0021-9533issn
1477-9137pii
116/19/4021journal_volume
116pub_type
杂志文章abstract：:Delayed wound healing caused by inefficient re-epithelialization underlines chronic skin lesions such as those found in diabetes. The dioxin receptor (AhR) modulates cell plasticity and migration and its activation by occupational polycyclic aromatic hydrocarbons (PAHs) results in severe skin lesions such as contact h...
journal_title：Journal of cell science
pub_type： 杂志文章
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journal_title：Journal of cell science
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abstract：:In a wild population of the American newt Notophthalmus viridescens 15 females out of a total of 94 were found to be heterozygous for a paracentric inversion which includes almost the whole of the longer arm of the smallest chromosome (XI). The inversion was recognized in preparations of lampbrush chromosomes because ...
journal_title：Journal of cell science
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更新日期：2010-01-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2007-08-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2004-09-15 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1987-11-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2019-08-23 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1981-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1990-11-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
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更新日期：2018-11-05 00:00:00
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journal_title：Journal of cell science
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更新日期：2000-01-01 00:00:00
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doi：
更新日期：2002-09-01 00:00:00
abstract：:Interferon regulatory factor 6 (Irf6) regulates keratinocyte proliferation and differentiation. In this study, we tested the hypothesis that Irf6 regulates cellular migration and adhesion. Irf6-deficient embryos at 10.5 days post-conception failed to close their wound compared with wild-type embryos. In vitro, Irf6-de...
journal_title：Journal of cell science
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更新日期：2014-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-05-01 00:00:00
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更新日期：2016-08-01 00:00:00
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更新日期：2000-07-01 00:00:00
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