Abstract:
:Human erythrocytes suspended in buffered isotonic saline were frozen to the temperature of liquid nitrogen at various cooling rates of 3, 140, 700, 1800, 3500, 8000 and 11 500 deg. C/min. The membrane ultrastructure in the frozen state and the extent of haemolysis after thawing were examined at each cooling rate. As the cooling rates increased from 3 to 3500 deg. C/min, the extent of lysis gradually decreased, but further increase in cooling rates in excess of 8000 deg. C/min resulted in an abrupt increase of lysis. Membrane-associated vesicles devoid of intramembrane particles (IMPs) were formed in the erythrocyte membranes frozen at cooling rates slower than 1800 deg. C/min. The frequency and size of these vesicles were highly cooling-rate-dependent and they were no longer formed in the erythrocyte membranes frozen at cooling rates faster than 3500 deg. C/min. Another membrane ultrastructural change associated closely with the formation of intracellular ice crystals appeared at cooling rates faster than 8000 deg. C/min. The membrane regions in direct contact with intracellular ice crystals were physically damaged and had an appearance resembling worm-eaten spots. The erythrocytes frozen at a cooling rate of 3500 deg. C/min exhibited ultrastructural integrity of the membrane by avoiding the membrane changes caused by either slow or fast freezing. It is suggested, from the close relation between membrane ultrastructure and the extent of haemolysis, that the ultrastructural integrity of membrane in the frozen state is important for avoiding haemolysis after thawing, and that the membrane ultrastructural changes caused by both slow and fast freezing were responsible for the lysis after thawing.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Fujikawa Ssubject
Has Abstractpub_date
1981-06-01 00:00:00pages
369-82eissn
0021-9533issn
1477-9137journal_volume
49pub_type
杂志文章abstract::Adhesion between cells is established by the formation of specialized intercellular junctional complexes, such as desmosomes. Desmosomes contain isoforms of two members of the cadherin superfamily of cell adhesion proteins, desmocollins (Dsc) and desmogleins (Dsg), but their combinatorial roles in desmosome assembly a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.146316
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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更新日期:2002-11-01 00:00:00
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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更新日期:2000-05-01 00:00:00
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更新日期:2013-06-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02988
更新日期:2006-06-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2003-12-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2013-04-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2009-08-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2006-09-15 00:00:00
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更新日期:2012-03-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Journal of cell science
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更新日期:1975-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-05-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1978-10-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-03-01 00:00:00
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journal_title:Journal of cell science
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更新日期:2014-11-15 00:00:00
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更新日期:2019-10-03 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1996-10-01 00:00:00
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