Abstract:
:We have used the H-7w subclone of a Chinese hamster ovary cell line (K1) to investigate the role of cell surface architecture (specifically microvilli, blebs, and sheets) in determining the relative agglutinability of a cell line with Con A. Our evidence clearly demonstrates that no specific, immediately recognizable surface architecture is associated with the agglutinable or non-agglutinable phenotype. Our data suggest that the expression of microvilli on the cell surface is neither necessary to nor sufficient for the phenotype described by enhanced agglutinability with Con A. Furthermore our work demonstrates that cells covered with blebs are as agglutinable as cells covered with microvilli thereby suggesting that the intertwining of microvilli may not be an essential facet of the agglutination phenomenon.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Noonan KD,Ukena Tsubject
Has Abstractpub_date
1978-12-01 00:00:00pages
103-15eissn
0021-9533issn
1477-9137journal_volume
34pub_type
杂志文章abstract::Triton X-100-treated sperms were injected into non-nucleate egg fragments of Xenopus laevis to determine whether the structure of the injected sperm nucleus is affected by the cyclic activity of the cytoplasm. Swollen vesicular nuclei were very frequently observed when the sperms were injected and incubated during the...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1983-09-01 00:00:00
abstract::The hedgehog (Hh) signaling pathway plays a very important role in metazoan development by controlling pattern formation. Malfunction of the Hh signaling pathway leads to numerous serious human diseases, including congenital disorders and cancers. The seven-transmembrane domain protein Smoothened (Smo) is a key transd...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.128603
更新日期:2013-09-15 00:00:00
abstract::Sustained neurotransmission requires the tight coupling of synaptic vesicle (SV) exocytosis and endocytosis. The mechanisms underlying this coupling are poorly understood. We tested the hypothesis that a cGMP-dependent protein kinase (PKG), encoded by the foraging (for) gene in Drosophila melanogaster, is critical for...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.227165
更新日期:2019-04-05 00:00:00
abstract::During muscle development myosin molecules form symmetrical thick filaments, which integrate with the thin filaments to produce the regular sarcomeric lattice. In Drosophila indirect flight muscles (IFMs) the details of this process can be studied using genetic approaches. The weeP26 transgenic line has a GFP-encoding...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.110361
更新日期:2013-01-01 00:00:00
abstract::An acrosome reaction was induced in ejaculated ram spermatozoa by treatment with calcium and the ionophore A23187. Samples were fixed at different times after initiation of induction, and the morphological changes within the head membranes that took place as exocytosis occurred were studied in freeze-fracture replicas...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1986-03-01 00:00:00
abstract::Electron-microscope autoradiography has been used to obtain information on the localization of DNA labelled with [3H]thymidine in chloroplasts known to be replicating and concomitantly synthesizing and segregating DNA, in cultured leaf disks. The studies were made using both Microdol-X developer and a 'compact' develo...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1976-03-01 00:00:00
abstract::Using a polyclonal antibody against purified zymogen granule membrane components from rat pancreas a cDNA coding for the 29 kDa protein (ZG29p) was identified by immunoscreening of a hormonally stimulated pancreas cDNA library. Western blot analysis suggests that ZG29p is a pancreas-specific protein and immunofluoresc...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::Antithrombin inhibits chemokine-induced migration of neutrophils by activating heparan sulfate proteoglycan-dependent signaling. Whether antithrombin affects migration of other types of leukocytes is not known. We investigated the effects of antithrombin on spontaneous and chemokine-triggered migration of lymphocytes ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::The interaction of promastigotes of the protozoan parasite Leishmania tropica with mouse peritoneal macrophages in vitro was studied by scanning electron microscopy. Motile promastigotes attached to host cells by their flagellar tips to which the macrophages responded by producing rather closely fitting lamellar sheat...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1979-10-01 00:00:00
abstract::The tetrapeptide N-Acetyl-Seryl-Aspartyl-Lysyl-Proline (AcSDKP) is a natural regulator of hematopoietic stem cell proliferation. The present study was aimed at investigating the presence and the role of AcSDKP in rat testis. Specific immunoreactivity was always observed in the interstitial tissue at all stages of test...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::In Saccharomyces cerevisiae, three classes of sphingolipids contain myo-inositol--inositol phosphorylceramide (IPC), mannosylinositol phosphorylceramide (MIPC) and mannosyldiinositol phosphorylceramide [M(IP)(2)C]. No fission yeast equivalent of Ipt1p, the inositolphosphotransferase that synthesizes M(IP)(2)C from MIP...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.059139
更新日期:2010-05-01 00:00:00
abstract::We report here an analysis of the execution point of the temperature-sensitive Saccharomyces cerevisiae cell cycle mutant, cdc27-47. When a logarithmically growing culture was shifted from standard growth conditions (strain 27.8B growing in YEPD at 25 degrees C) to the restrictive temperature cell division ceased abru...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
abstract::Although the 11.5 kDa Zn(2+)-binding protein (ZnBP, parathymosin-alpha) possesses a functional bipartite nuclear localization signal it was found in most tissues in the cytoplasm. The cultivation of freshly isolated rat hepatocytes for 24 hours under standard conditions was associated with an almost complete transloca...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract::We investigated the effect of glucose deprivation treatment on clonogenicity in multidrug-resistant human breast carcinoma MCF-7/ADR cells. Survival of MCF-7/ADR cells decreased exponentially up to 8 hours of incubation in the glucose-free medium. The surviving fraction of these cells for 8 hours of glucose-deprivatio...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
abstract::We previously reported that the FLA10 locus on the uni linkage group of Chlamydomonas encodes a kinesin homologous protein, KHP1. The fla10 phenotype, which is a temperature-sensitive defect for flagellar assembly and maintenance, is rescued by transformation with the wild-type KHP1 gene. In the present study we ident...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-03-01 00:00:00
abstract::In response to endovascular injury, platelet-derived growth factor-BB (PDGF-BB) and basic fibroblast growth factor (bFGF) are released locally and modulate vascular smooth muscle cells (SMC) proliferation and migration within the vascular wall. The aim of the present in vitro study was to determine how rat aorta SMC r...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract::This study uses electron tomography linked to a variety of other EM methods to provide an integrated view of the flagellar pocket and basal body area of the African trypanosome procyclic trypomastigote. We reveal the pocket as an asymmetric membranous 'balloon' with two boundary structures. One of these - the collar -...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.045740
更新日期:2009-04-15 00:00:00
abstract::Normal human foreskin keratinocytes (HFKs) and transformed HFKs (FEPE1L-8 cells) generated by the introduction of cloned human papillomavirus type 16 sequences were compared for the expression and function of a family of adhesion receptors termed integrins. Initially, cells were examined in conventional monolayer cult...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00
abstract::Actin filaments assemble into diverse protrusive and contractile structures to provide force for a number of vital cellular processes. Stress fibers are contractile actomyosin bundles found in many cultured non-muscle cells, where they have a central role in cell adhesion and morphogenesis. Focal-adhesion-anchored str...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.098087
更新日期:2012-04-15 00:00:00
abstract::In animals and yeast, CLASP proteins are microtubule plus-end tracking proteins (+TIPS) involved in the regulation of microtubule plus-end dynamics and stabilization. Here we show that mutations in the Arabidopsis CLASP homolog result in various plant growth reductions, cell form defects and reduced mitotic activity. ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.024950
更新日期:2007-12-15 00:00:00
abstract::Two monoclonal antibodies, BA16 and BA17, have been developed using a detergent-insoluble extract of human mammary epithelial organoids as immunogen. Indirect immunofluorescent staining of cultured cells showed that the component reacting with the antibodies was filamentous and the intensity of staining was stronger i...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1985-04-01 00:00:00
abstract::The contractile vacuole complex (CVC) of Trypanosoma cruzi, the etiologic agent of Chagas disease, collects and expels excess water as a mechanism of regulatory volume decrease after hyposmotic stress; it also has a role in cell shrinking after hyperosmotic stress. Here, we report that, in addition to its role in osmo...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.169466
更新日期:2015-06-15 00:00:00
abstract::The fate of concanavalin A (Con A) bound to normal and enucleated L cells was followed at the ultrastructural level over a 20-h period. In both enucleates and normal cells the Con A is seen to be distributed in a uniform manner over the entire cell surface following a 30-min pulse with a low concentration of Con A. In...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1976-12-01 00:00:00
abstract::Although membrane addition is crucial for cytokinesis in many animal cell types, the specific mechanisms supporting cleavage furrow ingression are not yet understood. Mutations in the gene brunelleschi (bru), which encodes the Drosophila ortholog of the yeast Trs120p subunit of TRAPPII, cause failure of furrow ingress...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.054536
更新日期:2009-12-15 00:00:00
abstract::It is becoming clear that mechanical stimuli are crucial factors in regulating the biology of the cell, but the short-term structural response of a cell to mechanical forces remains relatively poorly understood. We mechanically stimulated cells transiently expressing actin-EGFP with controlled forces (0-20 nN) in orde...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.088302
更新日期:2012-02-01 00:00:00
abstract::Pieces of small intestine from newborn, I- and 6-day-old piglets were incubated in vitro and ligated segments were incubated in vivo with polycationic ferritin (PCF) to determine the distribution and possible role in protein uptake of anionic sites on membranes at the luminal surfaces of the epithelial cells. Most PCF...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract::A tissue culture system for epithelial cells derived from male human foetal genital ducts has been established. The cells show morphological and biochemical characteristics of ductal epithelial cells, and can be passaged and maintained in culture for considerable periods of time. These cells will provide a suitable sy...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1989-04-01 00:00:00
abstract::The large GTPase dynamin is a mechanoenzyme that participates in the scission of nascent vesicles from the plasma membrane. Recently, dynamin has been demonstrated to associate with the Golgi apparatus in mammalian cells by morphological and biochemical methods. Additional studies using a well characterized, cell-free...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract::Wound healing crucially relies on the mechanical activity of fibroblasts responding to TGFβ1 and to forces transmitted across focal adhesions. Integrin-linked kinase (ILK) is a central adapter recruited to integrin β1 tails in focal adhesions mediating the communication between cells and extracellular matrix. Here, we...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.063024
更新日期:2010-11-15 00:00:00
abstract::Cytokinesis in many eukaryotes requires a contractile actomyosin ring that is placed at the division site. In fission yeast, which is an attractive organism for the study of cytokinesis, actomyosin ring assembly and contraction requires the myosin II heavy chain Myo2p. Although myo2-E1, a temperature-sensitive mutant ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.205625
更新日期:2018-01-04 00:00:00