Different effects of protein kinase inhibitors on the localization of junctional proteins at cell-cell contact sites.

abstract：:The protozoan parasite Giardia lamblia has the unusual morphology of bearing two equal-sized nuclei. This organism probably represents the earliest diverging lineage of eukaryotes, suggesting that its biological tactics may be transitional. To begin to understand the role played by the two equal-sized nuclei in this o...

journal_title：Journal of cell science

authors： Kabnick KS,Peattie DA

更新日期：1990-03-01 00:00:00

abstract：:Polyethylene glycol (PEG) was used to fuse trout sperm with Chinese hamster fibroblasts (CHW-1102). As judged by light microscopy, PEG significantly increased the number of associations between the two cell types. Electron microscopy revealed that the sperm nuclei were in the cytoplasm of CHW-1102 in cultures that had...

journal_title：Journal of cell science

authors： Yip JH,Bols NC

更新日期：1982-02-01 00:00:00

abstract：:Development of the nematocyst-taeniocyst complex in the four-zooid stage of a dinoflagellate, Polykrikos kofoidi, was studied by electron microscopy. We observed the following stages: formation of large spherical bodies in islets of cytoplasm containing extensive rough endoplasmic reticulum and Golgi complexes; differ...

journal_title：Journal of cell science

authors： Westfall JA,Bradbury PC,Townsend JW

更新日期：1983-09-01 00:00:00

abstract：:In the present study, to further elucidate the molecular events that control neural crest cell migration, we have analyzed in vitro the adhesive and locomotory response of avian trunk neural crest cells to laminin-1 and searched for the integrin receptors involved in this process. Adhesion of crest cells on laminin-1 ...

journal_title：Journal of cell science

authors： Desban N,Duband JL

更新日期：1997-11-01 00:00:00

abstract：:Gap junctions (GJs) assembled from connexin (Cx) proteins allow direct cell-cell communication. While phosphorylation is known to regulate multiple GJ functions, much less is known about the role of ubiquitin in these processes. Using ubiquitylation-type-specific antibodies and Cx43 lysine-to-arginine mutants we show ...

journal_title：Journal of cell science

authors： Kells-Andrews RM,Margraf RA,Fisher CG,Falk MM

更新日期：2018-08-09 00:00:00

abstract：:Dynamin-related GTPase proteins (DRPs) are main players in membrane remodelling. Conserved DRPs called mitofusins (Mfn1/Mfn2/Fzo1) mediate the fusion of mitochondrial outer membranes (OM). OM fusion depends on self-assembly and GTPase activity of mitofusins as well as on two other proteins, Ugo1 and Mdm30. Here, we de...

journal_title：Journal of cell science

authors： Anton F,Fres JM,Schauss A,Pinson B,Praefcke GJ,Langer T,Escobar-Henriques M

更新日期：2011-04-01 00:00:00

abstract：:Cancer cells thrive when challenged with proteotoxic stress by inducing components of the protein folding, proteasome, autophagy and unfolded protein response (UPR) pathways. Consequently, specific molecular chaperones have been validated as targets for anti-cancer therapies. For example, inhibition of Hsp70 family pr...

journal_title：Journal of cell science

authors： Sannino S,Guerriero CJ,Sabnis AJ,Stolz DB,Wallace CT,Wipf P,Watkins SC,Bivona TG,Brodsky JL

更新日期：2018-09-05 00:00:00

abstract：:The events that accompany sporogenesis in the apogamous fern Dryopteris borreri parallel those seen in sexually reproducing ferns. Organelles dedifferentiate and redifferentiate, and form a discrete band across the equator of dyads; nuclear vacuoles and lipid spherosomes appear during prophase, and the major part of t...

journal_title：Journal of cell science

authors： Sheffield E,Laird S,Bell PR

更新日期：1983-09-01 00:00:00

abstract：:Targeting of glycosylphosphatidylinositol-anchored proteins to the apical surface of epithelial cells involves clustering in Triton X-100-resistant membrane microdomains or rafts. The role of these microdomains in sorting transmembrane proteins is more questionable because, unlike glycosylphosphatidylinositol-anchored...

journal_title：Journal of cell science

authors： Delaunay JL,Breton M,Goding JW,Trugnan G,Maurice M

更新日期：2007-03-15 00:00:00

abstract：:Isoamyl alcohol (IAA) induces a phenotype that resembles pseudohyphae in the budding yeast Saccharomyces cerevisiae. We show here that IAA causes the rapid formation of linear chains of anucleate buds, each of which is accompanied by the formation of a septin ring at its neck. This process requires the activity of Swe...

journal_title：Journal of cell science

authors： Martinez-Anaya C,Dickinson JR,Sudbery PE

更新日期：2003-08-15 00:00:00

abstract：:Spindle pole bodies in the fission yeast Schizosaccharomyces pombe are required during meiosis, not only for spindle formation but also for the assembly of forespore membranes. The spo15 mutant is defective in the formation of forespore membranes, which develop into spore envelopes. The spo15(+)gene encodes a protein ...

journal_title：Journal of cell science

authors： Ikemoto S,Nakamura T,Kubo M,Shimoda C

更新日期：2000-02-01 00:00:00

abstract：:MOR1, the Arabidopsis thaliana homologue of the Xenopus microtubule-associated protein MAP215, is required for spatial organization of the acentrosomal microtubule arrays of plant cells. To determine how loss of MOR1 function affects microtubule dynamics, we compared various parameters of microtubule dynamics in the t...

journal_title：Journal of cell science

authors： Kawamura E,Wasteneys GO

更新日期：2008-12-15 00:00:00

abstract：:The IGF-II mRNA-binding proteins (IMPs), which are composed of two RNA recognition motifs, (RRM) and four hnRNP K homology (KH) domains, have been implicated in subcytoplasmic localization of mRNAs during embryogenesis. The IMP family originated via two gene duplications before the divergence of vertebrates, and IMP h...

journal_title：Journal of cell science

authors： Nielsen FC,Nielsen J,Kristensen MA,Koch G,Christiansen J

更新日期：2002-05-15 00:00:00

abstract：:Axenically grown cells of D. discoideum Ax-2 harvested in the log phase of growth, cohere rapidly when shaken in phosphate buffer. After 3.5 days in the stationary phase of growth, cells become completely non-cohesive. Although they do not stick to each other, stationary phase cells do stick to both log phase cells an...

journal_title：Journal of cell science

authors： Jaffe AR,Swan AP,Garrod DR

更新日期：1979-06-01 00:00:00

abstract：:We have characterised the functional regulation of the type-3 ryanodine receptor by the 12 kDa FK506-binding protein. Wild-type type-3 ryanodine receptor and mutant type-3 ryanodine receptor in which the critical valine at position 2322 in the central 12 kDa FK506-binding protein binding site was substituted by aspart...

journal_title：Journal of cell science

authors： Van Acker K,Bultynck G,Rossi D,Sorrentino V,Boens N,Missiaen L,De Smedt H,Parys JB,Callewaert G

更新日期：2004-03-01 00:00:00

abstract：:The lymphatic endothelial cell (LEC) fate decision program during development has been described. However, the mechanism underlying the maintenance of differentiated LEC identity remains largely unknown. Here, we show that fibroblast growth factor 2 (FGF2) plays a fundamental role in maintaining a differentiated LEC t...

journal_title：Journal of cell science

authors： Ichise T,Yoshida N,Ichise H

更新日期：2014-02-15 00:00:00

abstract：:In many epithelial cells, epidermal growth factor (EGF) augments the epithelial-mesenchymal transition (EMT) that occurs when cells are treated with transforming growth factor β (TGFβ). We demonstrate that this augmentation requires activation of SH2 domain-containing phosphatase-2 (SHP2; also known as PTPN11), a prot...

journal_title：Journal of cell science

authors： Buonato JM,Lan IS,Lazzara MJ

更新日期：2015-11-01 00:00:00

abstract：:Laminins are heterotrimeric glycoproteins of the extracellular matrix that are secreted by epithelial cells and which are crucial for the normal structure and function of the basement membrane. We have generated a mouse harboring a conditional knockout of α3 laminin (Lama3(fl/fl)), one of the main laminin subunits in ...

journal_title：Journal of cell science

authors： Urich D,Eisenberg JL,Hamill KJ,Takawira D,Chiarella SE,Soberanes S,Gonzalez A,Koentgen F,Manghi T,Hopkinson SB,Misharin AV,Perlman H,Mutlu GM,Budinger GR,Jones JC

更新日期：2011-09-01 00:00:00

abstract：:Prions are proteins that access self-templating amyloid forms, which confer phenotypic changes that can spread from individual to individual within or between species. These infectious phenotypes can be beneficial, as with yeast prions, or deleterious, as with mammalian prions that transmit spongiform encephalopathies...

journal_title：Journal of cell science

authors： Cushman M,Johnson BS,King OD,Gitler AD,Shorter J

更新日期：2010-04-15 00:00:00

abstract：:IGFBP3 is a multi-functional protein that has IGF-dependent and IGF-independent actions in cultured cells. Here we show that the IGF binding domain (IBD), nuclear localization signal (NLS) and transactivation domain (TA) are conserved and functional in zebrafish Igfbp3. The in vivo roles of these domains were investig...

journal_title：Journal of cell science

authors： Zhong Y,Lu L,Zhou J,Li Y,Liu Y,Clemmons DR,Duan C

更新日期：2011-06-01 00:00:00

abstract：:There is a limited number of methods to examine transbilayer lipid distribution in biomembranes. We employed freeze-fracture replica-labelling immunoelectron microscopy in combination with lipid-binding proteins and a peptide to examine both transbilayer distribution and lateral distribution of various phospholipids i...

journal_title：Journal of cell science

authors： Murate M,Abe M,Kasahara K,Iwabuchi K,Umeda M,Kobayashi T

更新日期：2015-04-15 00:00:00

abstract：:The 5T4 oncofoetal antigen is a 72 kDa glycoprotein defined by a monoclonal antibody raised against human placental trophoblast and is expressed in many different carcinomas but detected only at low levels in some normal epithelia. Immunohistochemical analysis of the patterns of expression in colorectal carcinomas has...

journal_title：Journal of cell science

authors： Carsberg CJ,Myers KA,Evans GS,Allen TD,Stern PL

更新日期：1995-08-01 00:00:00

abstract：:alphaA-Crystallin (alphaA) is a molecular chaperone expressed preferentially in the lens. alphaA transcripts are first detected during the early stages of lens development and its synthesis continues as the lens grows throughout life. alphaA(-/-) mouse lenses are smaller than controls, and lens epithelial cells derive...

journal_title：Journal of cell science

authors： Xi JH,Bai F,Andley UP

更新日期：2003-03-15 00:00:00

abstract：:Adhesion between cells is established by the formation of specialized intercellular junctional complexes, such as desmosomes. Desmosomes contain isoforms of two members of the cadherin superfamily of cell adhesion proteins, desmocollins (Dsc) and desmogleins (Dsg), but their combinatorial roles in desmosome assembly a...

journal_title：Journal of cell science

authors： Lowndes M,Rakshit S,Shafraz O,Borghi N,Harmon RM,Green KJ,Sivasankar S,Nelson WJ

更新日期：2014-05-15 00:00:00

abstract：:Cyclin E is a regulatory subunit of the cdc2-related protein kinase cdk2, which is activated shortly before S-phase entry, thus defining it as a G1 cyclin. We report here the existence of a 43 kDa splice variant of human cyclin E, termed cyclin Es, which lacks 49 amino acids within the cyclin box compared to the known...

journal_title：Journal of cell science

authors： Sewing A,Rönicke V,Bürger C,Funk M,Müller R

更新日期：1994-02-01 00:00:00

abstract：:Vaccinia virus B1 kinase plays a key role in viral DNA replication. The homologous mammalian vaccinia-related kinases (VRKs) are also implicated in the regulation of DNA replication, although direct evidence remains elusive. Here we show that VRK1 regulates cell cycle progression in the DNA replication period by induc...

journal_title：Journal of cell science

authors： Kang TH,Park DY,Kim W,Kim KT

更新日期：2008-09-15 00:00:00

abstract：:Crosstalk between the microtubule (MT) and actin cytoskeletons is fundamental to many cellular processes including cell polarisation and cell motility. Previous work has shown that members of the growth-arrest-specific 2 (GAS2) family mediate the crosstalk between filamentous actin (F-actin) and MTs, but the molecular...

journal_title：Journal of cell science

authors： Stroud MJ,Nazgiewicz A,McKenzie EA,Wang Y,Kammerer RA,Ballestrem C

更新日期：2014-06-15 00:00:00

abstract：:The bimE (blocked-in-mitosis) gene appears to function as a negative mitotic regulator because the recessive bimE7 mutation can override certain interphase-arresting treatments and mutations, causing abnormal induction of mitosis. We have further investigated the role of bimE in cell cycle checkpoint control by: (1) c...

journal_title：Journal of cell science

authors： James SW,Mirabito PM,Scacheri PC,Morris NR

更新日期：1995-11-01 00:00:00

abstract：:Several cell types express inducible nitric oxide synthase (NOS2) in response to exogenous insults such as bacterial lipopolysaccharide (LPS) or proinflammatory cytokines. For instance, muscular cells treated with LPS and interferon gamma (IFN-gamma) respond by increasing the mRNA and protein levels of NOS2, and synth...

journal_title：Journal of cell science

authors： Navarro-Lérida I,Portolés MT,Barrientos AA,Gavilanes F,Boscá L,Rodríguez-Crespo I

更新日期：2004-04-01 00:00:00

abstract：:How vesicle trafficking components actively contribute to regulation of paracrine signaling is unclear. We genetically uncovered a requirement for α-soluble NSF attachment protein (α-Snap) in the activation of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway during Drosophila egg de...

journal_title：Journal of cell science

authors： Saadin A,Starz-Gaiano M

更新日期：2018-11-30 00:00:00