Abstract:
:Cyclin E is a regulatory subunit of the cdc2-related protein kinase cdk2, which is activated shortly before S-phase entry, thus defining it as a G1 cyclin. We report here the existence of a 43 kDa splice variant of human cyclin E, termed cyclin Es, which lacks 49 amino acids within the cyclin box compared to the known 48 kDa cyclin E. Cyclin Es is expressed at approximately 1/10 of the level of full-length cyclin E in several cell lines analysed. The two cyclin E forms differ functionally in that cyclin E, but not cyclin Es, is able to complex with cdk2, to activate the histone H1, pRb and p107 in vitro kinase activity of cdk2 and to rescue a triple CLN mutation in S. cerevisiae. Cyclin Es is the first splice variant of a cell cycle regulatory protein to be described. Our findings also indicate that the cyclin box in cyclin E mediates the interaction with cdk2.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Sewing A,Rönicke V,Bürger C,Funk M,Müller Rsubject
Has Abstractpub_date
1994-02-01 00:00:00pages
581-8eissn
0021-9533issn
1477-9137journal_volume
107 ( Pt 2)pub_type
杂志文章abstract::Although the long-term effects of all-trans retinoic acid (RA) on neuronal growth and differentiation have been intensively studied, nothing is known about its effect on synaptic transmission. Here we show that RA rapidly and specifically enhances the spontaneous acetylcholine release at developing neuromuscular synap...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01153
更新日期:2004-06-15 00:00:00
abstract::The Tec kinases represent the second largest family of mammalian non-receptor tyrosine kinases and are distinguished by the presence of distinct proline-rich regions and pleckstrin homology domains that are required for proper regulation and activation. Best studied in lymphocyte and mast cells, these kinases are crit...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:
更新日期:2002-08-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1979-10-01 00:00:00
abstract::Transcription factor E2F plays an important role in coordinating and integrating early cell cycle progression with the transcription apparatus. It is known that physiological E2F arises when a member of two families of proteins, E2F and DP, interact as E2F/DP heterodimers and that transcriptional activity is regulated...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00
abstract::Activation of the N-formyl peptide receptor (FPR) of human neutrophils by ligands such as N-formyl-methionine-leucine-phenylalanine (fMLP) induces mobilization of intracellular calcium, cell adhesion, chemotaxis, superoxide production and degranulation. Chinese hamster ovary (CHO) cells are normally devoid of FPR and ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-07-30 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2007-03-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.00937
更新日期:2004-01-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.040162
更新日期:2009-07-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1980-04-01 00:00:00
abstract::Membrane-type matrix metalloproteinase 1 (MT1-MMP) is a membrane-anchored matrix metalloproteinase (MMP) that is frequently associated with processes involving tissue remodelling and cell migration. We have examined MT1-MMP expression and subcellular distribution as a function of MCF10A mammary epithelial cell migrati...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-08-01 00:00:00
abstract::The inclusion of lymphocytes in high endothelial venules and their migration to the lymph nodes are critical steps in the immune response. Cell migration is regulated by the actin cytoskeleton and myosins. Myo1e is a long-tailed class I myosin and is highly expressed in B cells, which have not been studied in the cont...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.235275
更新日期:2020-03-02 00:00:00
abstract::Fine-tuning of NMDA glutamatergic receptor signalling strategically controls crucial brain functions. This process depends on several ligands and modulators, one of which unexpectedly includes the serine protease tissue-type plasminogen activator (tPA). In vitro, tPA increases NMDA-receptor-mediated calcium influx by ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03354
更新日期:2007-02-15 00:00:00
abstract::SH4 domains provide bipartite membrane-targeting signals for oncogenic Src family kinases. Here we report the induction of non-apoptotic plasma membrane (PM) blebbing as a novel and conserved activity of SH4 domains derived from the prototypic Src kinases Src, Fyn, Yes and Lck as well as the HASPB protein of Leishmani...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.011130
更新日期:2007-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.098087
更新日期:2012-04-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01720
更新日期:2005-03-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:
更新日期:2002-06-01 00:00:00
abstract::Meiosis is characterized by two chromosome segregation rounds (meiosis I and II), which follow a single round of DNA replication, resulting in haploid genome formation. Chromosome reduction occurs at meiosis I. It relies on key structures, such as chiasmata, which are formed by repair of double-strand breaks (DSBs) be...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.152520
更新日期:2014-11-01 00:00:00
abstract::Previously we have shown that the Saccharomyces cerevisiae CCZ1 (YBR131w) gene encodes a protein involved in protein trafficking. Deletion of this gene leads to fragmentation of the vacuole typical of the class B vps mutants. Genetic and biochemical data indicated that Ccz1p is required for fusion of various transport...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.102327
更新日期:2012-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1977-08-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-02-01 00:00:00
abstract::Skeletal and heart muscle-specific variant of the α subunit of nascent polypeptide associated complex (skNAC; encoded by NACA) is exclusively found in striated muscle cells. Its function, however, is largely unknown. Previous reports have demonstrated that skNAC binds to m-Bop/Smyd1, a multi-functional protein that re...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.150334
更新日期:2014-09-01 00:00:00
abstract::Endoblast and hypoblast tissue, dissected from early chick embryos, was explanted and cultured on glass or plastic substrata. These tissues grew rapidly to form epithelial sheets. Under the same conditions, mesoderm, dissected without the aid of dissociating agents, grew poorly. After 24 h in culture, the mesoderm exp...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::The co-repressor proteins SMRT and NCoR concentrate in specific subnuclear compartments and function with DNA-binding factors to inhibit transcription. To provide detailed mechanistic understanding of these activities, this study tested the hypothesis that functional interactions with transcription factors, such as th...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02450
更新日期:2005-08-01 00:00:00
abstract::During early stages of meiotic prophase I the nucleus undergoes considerable reorganization, including the clustering of telomeres, the release of contacts between chromosomes and the nuclear membrane, the reorganization of the nucleolus, and chromatin remodeling. Using a light squashing technique for the analysis of ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03155
更新日期:2006-09-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1985-02-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2011-08-01 00:00:00