Abstract：
:The inclusion of lymphocytes in high endothelial venules and their migration to the lymph nodes are critical steps in the immune response. Cell migration is regulated by the actin cytoskeleton and myosins. Myo1e is a long-tailed class I myosin and is highly expressed in B cells, which have not been studied in the context of cell migration. By using intravital microscopy in an in vivo model and performing in vitro experiments, we studied the relevance of Myo1e for the adhesion and inclusion of activated B cells in high endothelial venules. We observed reduced expression of integrins and F-actin in the membrane protrusions of B lymphocytes, which might be explained by deficiencies in vesicular trafficking. Interestingly, the lack of Myo1e reduced the phosphorylation of focal adhesion kinase (FAK; also known as PTK2), AKT (also known as AKT1) and RAC-1, disturbing the FAK-PI3K-RAC-1 signaling pathway. Taken together, our results indicate a critical role of Myo1e in the mechanism of B-cell adhesion and migration.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Girón-Pérez DA,Vadillo E,Schnoor M,Santos-Argumedo Ldoi
10.1242/jcs.235275subject
Has Abstractpub_date
2020-03-02 00:00:00issue
5eissn
0021-9533issn
1477-9137pii
jcs.235275journal_volume
133pub_type
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