Abstract:
:Polyethylene glycol (PEG) was used to fuse trout sperm with Chinese hamster fibroblasts (CHW-1102). As judged by light microscopy, PEG significantly increased the number of associations between the two cell types. Electron microscopy revealed that the sperm nuclei were in the cytoplasm of CHW-1102 in cultures that had been treated with PEG and in phagosomes of CHW-1102 in cultures not treated with PEG. In the cytoplasm many sperm nuclei had an extensive network of fibres whereas before fusion the sperm nuclei contained chromatin blocks which were packed together tightly. In phagosomes they consisted of dispersed blocks of chromatin. The chromatin of the few sperm that were found outside CHW-1100 cells but in the same culture that was treated with PEG also lacked fibres and consisted of loosely packed blocks. Most nuclei were unaltered by PEG treatment alone although in a few the packing of the chromatin blocks was loosened. Thus the decondensation of sperm nuclei within CHW-1102 cells appears to be brought about by the mammalian cytoplasm rather than by digestion in phagosomes, the culture conditions, or the treatment used to induce fusion.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Yip JH,Bols NCsubject
Has Abstractpub_date
1982-02-01 00:00:00pages
307-21eissn
0021-9533issn
1477-9137journal_volume
53pub_type
杂志文章abstract::Cells need to sense their mechanical environment during the growth of developing tissues and maintenance of adult tissues. The concept of force-sensing mechanisms that act through cell-cell and cell-matrix adhesions is now well established and accepted. Additionally, it is widely believed that force sensing can be med...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.213496
更新日期:2019-07-30 00:00:00
abstract::The fractionation of human liver mitochondria into inner membrane, outer membrane and matrix material is reported. Compared with rat, human liver mitochondria are more fragile. Fractionation can be achieved in only 2 steps, a digitonin treatment for removal of the outer membrane and centrifugation of the inner membran...
journal_title:Journal of cell science
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doi:
更新日期:1979-02-01 00:00:00
abstract::A simplified model of TIRF optics was used to quantitate the relative membrane/substratum separation distances from the spatial pattern of TIRF image brightness. Phase-contrast and total internal reflection fluorescence microscopy (TIRFM) images were collected of bovine aortic endothelial cells (BAEC) plated onto glas...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
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journal_title:Journal of cell science
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更新日期:1999-12-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-05-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2005-10-01 00:00:00
abstract::Two monoclonal antibodies, BA16 and BA17, have been developed using a detergent-insoluble extract of human mammary epithelial organoids as immunogen. Indirect immunofluorescent staining of cultured cells showed that the component reacting with the antibodies was filamentous and the intensity of staining was stronger i...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1985-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1985-03-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2004-02-29 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2020-05-18 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
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更新日期:2016-05-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
abstract::Spindle pole bodies in the fission yeast Schizosaccharomyces pombe are required during meiosis, not only for spindle formation but also for the assembly of forespore membranes. The spo15 mutant is defective in the formation of forespore membranes, which develop into spore envelopes. The spo15(+)gene encodes a protein ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02743
更新日期:2006-01-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.051649
更新日期:2010-11-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.216630
更新日期:2018-09-03 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.223883
更新日期:2019-02-01 00:00:00
abstract::3-Phosphoinositide-dependent protein kinase-1 (PDK1) and phospholipase C (PLC)γ1 are two key enzymes in signal transduction that control several intracellular processes. Despite the fact that PLCγ1 has been investigated for several years, the mechanisms of activation of this enzyme are still not completely clear. Simi...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.100511
更新日期:2012-07-01 00:00:00
abstract::Cells constantly adapt to various environmental changes and stresses. The way in which nutrient and stress levels in a cell feed back to control metabolism and growth are, unsurprisingly, extremely complex, as responding with great sensitivity and speed to the 'feast or famine, slack or stress' status of its environme...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.168922
更新日期:2015-06-15 00:00:00
abstract::Plastids in plants and algae evolved from the endosymbiotic integration of a cyanobacterium by a heterotrophic eukaryote. New plastids can only emerge through fission; thus, the synchronization of bacterial division with the cell cycle of the eukaryotic host was vital to the origin of phototrophic eukaryotes. Most of ...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.203414
更新日期:2018-01-29 00:00:00
abstract::Sustained neurotransmission requires the tight coupling of synaptic vesicle (SV) exocytosis and endocytosis. The mechanisms underlying this coupling are poorly understood. We tested the hypothesis that a cGMP-dependent protein kinase (PKG), encoded by the foraging (for) gene in Drosophila melanogaster, is critical for...
journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2019-04-05 00:00:00
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journal_title:Journal of cell science
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更新日期:2009-06-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01359
更新日期:2004-09-15 00:00:00
abstract::Experimentally introduced tension on kinetochores and their centromeres has been shown to stabilize kinetochore attachment to microtubules, modify kinetochore directional instability, and regulate cell-cycle progression into anaphase. In mitosis, kinetochore tension and the stretch of centromere chromatin are produced...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
abstract::Several studies have shown that altered gravity conditions influence mammalian cell growth and differentiation. The molecular mechanisms underlying these effects, however, remain relatively obscure. In this paper we show that microgravity reached in a sounding rocket strongly decreases epidermal growth factor (EGF)-in...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1990-09-01 00:00:00
abstract::An acrosome reaction was induced in ejaculated ram spermatozoa by treatment with calcium and the ionophore A23187. Samples were fixed at different times after initiation of induction, and the morphological changes within the head membranes that took place as exocytosis occurred were studied in freeze-fracture replicas...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1986-03-01 00:00:00
abstract::p63 (also known as TP63) is a transcription factor of the p53 family, along with p73. Multiple isoforms of p63 have been discovered and these have diverse functions encompassing a wide array of cell biology. p63 isoforms are implicated in lineage specification, proliferative potential, differentiation, cell death and ...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
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更新日期:2020-09-11 00:00:00
abstract::Onconase is an RNase with a very specific property because it is selectively toxic to transformed cells. This toxin is thought to recognize cell surface receptors, and the protection conferred by metabolic poisons against Onconase toxicity indicated that this RNase relies on endocytic uptake to kill cells. Nevertheles...
journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2007-04-15 00:00:00
abstract::Many pathways dysregulated in prostate cancer are also involved in epithelial differentiation. To better understand prostate tumor initiation, we sought to investigate specific genes and mechanisms required for normal basal to luminal cell differentiation. Utilizing human prostate basal epithelial cells and an in vitr...
journal_title:Journal of cell science
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更新日期:2017-06-01 00:00:00