Intracellular pathway of Onconase that enables its delivery to the cytosol.

abstract：:Histone proteins are essential for chromatin formation, and histone gene expression is coupled to DNA synthesis. In metazoans, the histone RNA binding protein HBP/SLBP is involved in post-transcriptional control of histone gene expression. In vitro assays have demonstrated that human HBP/SLBP is involved in histone mR...

journal_title：Journal of cell science

authors： Zhao X,McKillop-Smith S,Müller B

更新日期：2004-12-01 00:00:00

abstract：:Phosphoinositides regulate myriad cellular processes, acting as potent signaling molecules in conserved signaling pathways and as organelle gatekeepers that recruit effector proteins to membranes. Phosphoinositide-generating enzymes have been studied extensively in yeast and cultured cells, yet their roles in animal d...

journal_title：Journal of cell science

authors： Tan J,Oh K,Burgess J,Hipfner DR,Brill JA

更新日期：2014-03-01 00:00:00

abstract：:We reported previously that Ca2+ depletion of Dictyostelium discoideum cells severely inhibits extracellular cyclic nucleotide phosphodiesterase (PD) synthesis at a post-transcriptional step. In this study, further experiments were performed to learn more about the nature of this phenomenon. Examination of the polysom...

journal_title：Journal of cell science

authors： Coukell MB,Cameron AM,Adames NR

更新日期：1992-10-01 00:00:00

abstract：:Signalling by target-derived neurotrophins is essential for the correct development of the nervous system and its maintenance throughout life. Several aspects concerning the lifecycle of neurotrophins and their receptors have been characterised over the years, including the formation, endocytosis and trafficking of si...

journal_title：Journal of cell science

authors： Budzinska MI,Villarroel-Campos D,Golding M,Weston A,Collinson L,Snijders AP,Schiavo G

更新日期：2020-03-30 00:00:00

abstract：:Using a series of chimeric and truncated N-acetylglucosaminyltransferase I (NAGT I) molecules we have shown that part of the lumenal stalk region is both necessary and sufficient for kin recognition of mannosidase II and retention in the Golgi stack. The membrane-spanning domain was not required for retention, but rep...

journal_title：Journal of cell science

authors： Nilsson T,Rabouille C,Hui N,Watson R,Warren G

更新日期：1996-07-01 00:00:00

abstract：:During meiotic prophase in the ciliate Tetrahymena thermophila micronuclei dramatically elongate and form thread-like crescents. The arrangement of the chromosomes within the crescent as well as the timing of chromosome pairing and recombination with respect to the elongation process have been subjects of ongoing deba...

journal_title：Journal of cell science

authors： Loidl J,Scherthan H

更新日期：2004-11-15 00:00:00

abstract：:Gene expression of malaria parasites is mediated by the apicomplexan Apetala2 (ApiAP2) transcription factor family. Different ApiAP2s control gene expression at distinct stages in the complex life cycle of the parasite, ensuring timely expression of stage-specific genes. ApiAP2s recognize short cis-regulatory elements...

journal_title：Journal of cell science

authors： Klug D,Kehrer J,Frischknecht F,Singer M

更新日期：2018-10-22 00:00:00

abstract：:The establishment of the junctional complex in epithelial cells requires the presence of extracellular calcium, and is controlled by a network of reactions involving G-proteins, phospholipase C and protein kinase C. Since potential candidates for phosphorylation are the tight junction associated proteins ZO1, ZO2 and ...

journal_title：Journal of cell science

authors： Perez-Moreno M,Avila A,Islas S,Sanchez S,González-Mariscal L

更新日期：1998-12-01 00:00:00

abstract：:The chromosomes of the active previtellogenic oocytes of the dragonfly Cordulia aenea L. extrude into the cytoplasm a substance, which is subsequently found there in the form of granulofibrillar masses. These nuclear extrusions evolve ultrastructurally into two components, nematosomes and dense masses. The nematosomes...

journal_title：Journal of cell science

authors： Halkka L,Halkka O

更新日期：1975-10-01 00:00:00

abstract：:The spindle checkpoint detects errors in kinetochore attachment to microtubules and delays anaphase if attachment is improper. The checkpoint is activated by attachment-sensitive components including Mad2 and certain phosphorylated proteins detected by the 3F3/2 antibody. We have studied Mad2 and 3F3/2 immunofluoresce...

journal_title：Journal of cell science

authors： Nicklas RB,Waters JC,Salmon ED,Ward SC

更新日期：2001-12-01 00:00:00

abstract：:Clathrin-mediated endocytosis requires cargo-specific adaptor proteins that recognize specific receptors and recruit them into coated pits. ARH [also called low-density lipoprotein receptor (LDLR) adaptor protein] serves as an adaptor for LDLR endocytosis in liver. However, ARH is dispensable for LDL uptake by some ot...

journal_title：Journal of cell science

authors： Maurer ME,Cooper JA

更新日期：2006-10-15 00:00:00

abstract：:We have used MPM-2, a monoclonal antibody raised against mitotic HeLa cells, to stain a Drosophila cell line, whole mounts of Drosophila embryos, and sectioned tissue from embryonic and larval stages of development. MPM-2 recognizes a major phosphoprotein of approximately 125 X 10(3) Mr in Drosophila tissue culture ce...

journal_title：Journal of cell science

authors： Millar SE,Freeman M,Glover DM

更新日期：1987-02-01 00:00:00

abstract：:The cell surface delivery of extracellular matrix (ECM) and integrins is fundamental for cell migration in wound healing and during cancer cell metastasis. This process is not only driven by several soluble NSF attachment protein (SNAP) receptor (SNARE) proteins, which are key players in vesicle transport at the cell ...

journal_title：Journal of cell science

authors： Enrich C,Rentero C,Hierro A,Grewal T

更新日期：2015-03-15 00:00:00

abstract：:To understand the molecular mechanisms underlying sorting of apical and basolateral membrane components in human intestinal epithelial cells, we have cloned the human homolog of rat syntaxin 3 and looked for its subcellular localization. Endogenous human syntaxin 3 was found to be localized at the apical membrane of c...

journal_title：Journal of cell science

authors： Delgrossi MH,Breuza L,Mirre C,Chavrier P,Le Bivic A

更新日期：1997-09-01 00:00:00

abstract：:The mitochondrial influx and efflux of Ca(2+) play a relevant role in cytosolic and mitochondrial Ca(2+) homeostasis, and contribute to the regulation of mitochondrial functions in neurons. The mitochondrial Na(+)/Ca(2+) exchanger, which was first postulated in 1974, has been primarily investigated only from a functio...

journal_title：Journal of cell science

authors： Scorziello A,Savoia C,Sisalli MJ,Adornetto A,Secondo A,Boscia F,Esposito A,Polishchuk EV,Polishchuk RS,Molinaro P,Carlucci A,Lignitto L,Di Renzo G,Feliciello A,Annunziato L

更新日期：2013-12-15 00:00:00

abstract：:The JAK/STAT pathway is an essential signalling cascade required for multiple processes during development and for adult homeostasis. A key question in understanding this pathway is how it is regulated in different cell contexts. Here, we have examined how endocytic processing contributes to signalling by the single c...

journal_title：Journal of cell science

authors： Moore R,Vogt K,Acosta-Martin AE,Shire P,Zeidler M,Smythe E

更新日期：2020-10-12 00:00:00

abstract：:Talin binds to β-integrin tails to activate integrins, regulating cell migration, invasion and metastasis. There are two talin genes, TLN1 and TLN2, encoding talin1 and talin2, respectively. Talin1 regulates focal adhesion dynamics, cell migration and invasion, whereas the biological function of talin2 is not clear an...

journal_title：Journal of cell science

authors： Qi L,Jafari N,Li X,Chen Z,Li L,Hytönen VP,Goult BT,Zhan CG,Huang C

更新日期：2016-10-01 00:00:00

abstract：:We have evaluated a series of laminarin sulphates with different degrees of sulphation (0.3-2.3) as antagonists of basic fibroblast growth factor (bFGF) and as inhibitors of the bFGF-dependent endothelial cell line FBHE. Inhibition of binding of bFGF by the laminarin sulphates increased with increasing degree of sulph...

journal_title：Journal of cell science

authors： Hoffman R,Paper DH,Donaldson J,Alban S,Franz G

更新日期：1995-11-01 00:00:00

abstract：:Activity of AURKA is controlled through multiple mechanisms including phosphorylation, ubiquitin-mediated degradation and allosteric interaction with TPX2. Activity peaks at mitosis, before AURKA is degraded during and after mitotic exit in a process strictly dependent on the APC/C coactivator FZR1. We used FZR1 knock...

journal_title：Journal of cell science

authors： Abdelbaki A,Akman HB,Poteau M,Grant R,Gavet O,Guarguaglini G,Lindon C

更新日期：2020-06-16 00:00:00

abstract：:We have recently shown that leukotriene D(4) (LTD(4)) increases cell survival in intestinal epithelial cells. Here we report and explore the complementary finding that LTD(4) also enhances proliferation in these cells. This proliferative response was approximately half of that induced by epidermal growth factor (EGF) ...

journal_title：Journal of cell science

authors： Paruchuri S,Hallberg B,Juhas M,Larsson C,Sjölander A

更新日期：2002-05-01 00:00:00

abstract：:Vaccinia virus B1 kinase plays a key role in viral DNA replication. The homologous mammalian vaccinia-related kinases (VRKs) are also implicated in the regulation of DNA replication, although direct evidence remains elusive. Here we show that VRK1 regulates cell cycle progression in the DNA replication period by induc...

journal_title：Journal of cell science

authors： Kang TH,Park DY,Kim W,Kim KT

更新日期：2008-09-15 00:00:00

abstract：:8-Hydroxyquinoline (HQ) chelates Mg2+ and Mn2+ and, secondarily, affects the activities of DNA and RNA polymerases. The in vivo effect of HQ has been estimated in Allium cepa L. meristems growing under new growth kinetics in the presence of this agent. HQ (at both 5 X 10(-5) M and 10(-4) M) depressed incorporation of ...

journal_title：Journal of cell science

authors： Ferrero ML,De la Torre C

更新日期：1986-02-01 00:00:00

abstract：:The Schizosaccaromyces pombe protein Flp1p belongs to a conserved family of serine-threonine-phosphatases. The founding member of this family, Saccharomyces cerevisiae Cdc14p, is required for inactivation of mitotic CDKs and reversal of CDK mediated phosphorylation at the end of mitosis, thereby bringing about the M-G...

journal_title：Journal of cell science

authors： Esteban V,Blanco M,Cueille N,Simanis V,Moreno S,Bueno A

更新日期：2004-05-15 00:00:00

abstract：:Through serial thin-section analysis of rat mammary epithelial cells, the number of centrioles per cell and their intracellular location were determined. In all developmental stages (e.g. virginal, pregnant, lactation, involution), each epithelial cell contained a single centriole that was located in the apical region...

journal_title：Journal of cell science

authors： Dylewski DP,Keenan TW

更新日期：1984-12-01 00:00:00

abstract：:Mechanical cues are essential for the regulation of cell and tissue physiology. Hence, it has become an utmost necessity for cell biologists to account for those mechanical parameters when investigating biological processes and they need devices to manipulate cells accordingly. Here, we report a simple mechanical cell...

journal_title：Journal of cell science

authors： Boulter E,Tissot FS,Dilly J,Pisano S,Féral CC

更新日期：2020-01-06 00:00:00

abstract：:Membrane-bound transferrin-like protein (MTf), a glycosylphosphatidylinositol-anchored protein, is expressed at high levels in many tumors and in several fetal and adult tissues including cartilage and the intestine, as well as in the amyloid plaques of Alzheimer's disease, although its role remains unknown. MTf is on...

journal_title：Journal of cell science

authors： Oda R,Suardita K,Fujimoto K,Pan H,Yan W,Shimazu A,Shintani H,Kato Y

更新日期：2003-05-15 00:00:00

abstract：:Start is the main decision point in the eukaryotic cell cycle at which cells commit to a new round of cell division. It involves the irreversible activation of a transcriptional programme through the inactivation of Start transcriptional repressors: the retinoblastoma family in mammals, or Whi5 and its recently identi...

journal_title：Journal of cell science

authors： Méndez E,Gomar-Alba M,Bañó MC,Mendoza M,Quilis I,Igual JC

更新日期：2020-12-21 00:00:00

abstract：:The highly elongated bristles of Drosophila have proven to be a valuable model system for studying cellular morphogenesis. Extending bristles contain a series of large bundles of actin filaments juxtaposed to the plasma membrane and centrally located microtubules. Models to explain the extension of the bristle have pr...

journal_title：Journal of cell science

authors： Fei X,He B,Adler PN

更新日期：2002-10-01 00:00:00

abstract：:Fe-S clusters act as co-factors of proteins with diverse functions, for example, in DNA repair. Downregulation of the cytosolic iron-sulfur protein assembly (CIA) machinery promotes genomic instability through the inactivation of multiple DNA repair pathways. Furthermore, CIA deficiencies are associated with so far un...

journal_title：Journal of cell science

authors： Ben-Shimon L,Paul VD,David-Kadoch G,Volpe M,Stümpfig M,Bill E,Mühlenhoff U,Lill R,Ben-Aroya S

更新日期：2018-06-27 00:00:00

abstract：:Microtubules stimulate contractile-ring formation in the equatorial cortex and simultaneously suppress contractility in the polar cortex; how they accomplish these differing activities is incompletely understood. We measured the behavior of GFP-actin in mammalian cells treated with nocodazole under conditions that eit...

journal_title：Journal of cell science

authors： Murthy K,Wadsworth P

更新日期：2008-07-15 00:00:00