Abstract：
:Microtubules stimulate contractile-ring formation in the equatorial cortex and simultaneously suppress contractility in the polar cortex; how they accomplish these differing activities is incompletely understood. We measured the behavior of GFP-actin in mammalian cells treated with nocodazole under conditions that either completely eliminate microtubules or selectively disassemble astral microtubules. Selective disassembly of astral microtubules resulted in functional contractile rings that were wider than controls and had altered dynamic activity, as measured by FRAP. Complete microtubule disassembly or selective loss of astral microtubules resulted in wave-like contractile behavior of actin in the non-equatorial cortex, and mislocalization of myosin II and Rho. FRAP experiments showed that both contractility and actin polymerization contributed to the wave-like behavior of actin. Wave-like contractile behavior in anaphase cells was Rho-dependent. We conclude that dynamic astral microtubules function to suppress Rho activation in the non-equatorial cortex, limiting the contractile activity of the polar cortex.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Murthy K,Wadsworth Pdoi
10.1242/jcs.027052subject
Has Abstractpub_date
2008-07-15 00:00:00pages
2350-9issue
Pt 14eissn
0021-9533issn
1477-9137pii
jcs.027052journal_volume
121pub_type
杂志文章abstract：:Membrane contact sites (MCSs), where the membranes of two organelles are closely apposed, are regions where small molecules such as lipids or calcium are exchanged between organelles. We have identified a conserved membrane-binding domain found exclusively in proteins at MCSs in Saccharomyces cerevisiae. The synaptota...
journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2012-01-01 00:00:00
abstract：:Every two years, the French Society for Cell Biology (SBCF) organises an international meeting called 'Imaging the Cell'. This year, the 8th edition was held on 24-26 June 2015 at University of Bordeaux Campus Victoire in the city of Bordeaux, France, a UNESCO World Heritage site. Over the course of three days, the me...
journal_title：Journal of cell science
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journal_title：Journal of cell science
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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doi：
更新日期：1998-03-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2021-01-08 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2009-11-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2008-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01560
更新日期：2004-12-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2009-10-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.059196
更新日期：2009-11-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01287
更新日期：2004-08-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.036673
更新日期：2009-01-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-02-01 00:00:00
abstract：:The bimE (blocked-in-mitosis) gene appears to function as a negative mitotic regulator because the recessive bimE7 mutation can override certain interphase-arresting treatments and mutations, causing abnormal induction of mitosis. We have further investigated the role of bimE in cell cycle checkpoint control by: (1) c...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2014-01-15 00:00:00
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journal_title：Journal of cell science
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doi：
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更新日期：2013-11-01 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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doi：
更新日期：2002-04-15 00:00:00
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更新日期：2015-06-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-11-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章,评审
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更新日期：2012-10-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2005-06-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-10-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章,评审
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