Identification of PTPsigma as an autophagic phosphatase.

abstract：:An essential step in mammalian fertilisation is the sperm acrosome reaction (AR) - exocytosis of a single large vesicle (the acrosome) that surrounds the nucleus at the apical sperm head. The acrosomal and plasma membranes fuse, resulting in both the release of factors that might facilitate penetration of the zona pel...

journal_title：Journal of cell science

authors： Harper CV,Cummerson JA,White MR,Publicover SJ,Johnson PM

更新日期：2008-07-01 00:00:00

abstract：:The cytostome-cytopharynx complex is the main site for endocytosis in epimastigotes of Trypanosoma cruzi It consists of an opening at the plasma membrane surface - the cytostome - followed by a membrane invagination - the cytopharynx. In G1/S cells, this structure is associated with two specific sets of microtubules, ...

journal_title：Journal of cell science

authors： Alcantara CL,Vidal JC,de Souza W,Cunha-E-Silva NL

更新日期：2017-01-01 00:00:00

abstract：:Endocytic trafficking is regulated by ubiquitylation (also known as ubiquitination) of cargoes and endocytic machineries. The role of ubiquitylation in lysosomal delivery has been well documented, but its role in the recycling pathway is largely unknown. Here, we report that the ubiquitin (Ub) ligase RFFL regulates ub...

journal_title：Journal of cell science

authors： Sakai R,Fukuda R,Unida S,Aki M,Ono Y,Endo A,Kusumi S,Koga D,Fukushima T,Komada M,Okiyoneda T

更新日期：2019-02-07 00:00:00

abstract：:Endothelial cells are known to undergo transitions in cell shape during long-term culture. Thus, the assumption that the separate phenotypes of microvascular endothelial cells (MVEC) recently isolated from bovine corpus luteum represent constitutively different cell strains cannot automatically be made. For this reaso...

journal_title：Journal of cell science

authors： Fenyves AM,Behrens J,Spanel-Borowski K

更新日期：1993-11-01 00:00:00

abstract：:The biosynthetic machinery in the melanotrope cells of the Xenopus intermediate pituitary is primarily dedicated to the generation of proopiomelanocortin (POMC)-derived, melanophore-stimulating peptides. Transfer of the animal to a black background stimulates the production of these peptides and causes a dramatic incr...

journal_title：Journal of cell science

authors： Holthuis JC,Jansen EJ,van Riel MC,Martens GJ

更新日期：1995-10-01 00:00:00

abstract：:The effects of isolated plasma membranes on cohesion of Dictyostelium discoideum Ax-2 cells have been studied. Membranes isolated from cells in the log phase of growth gave complete inhibition of log-phase cell cohesion. This effect was specific for the cohesion log-phase cells mediated by contact sites B, since the c...

journal_title：Journal of cell science

authors： Jaffé AR,Garrod DR

更新日期：1979-12-01 00:00:00

abstract：:Previous reports have suggested that Cx26 exhibits unique intracellular transport pathways en route to the cell surface compared with other members of the connexin family. To directly examine and compare nascent and steady-state delivery of Cx43 and Cx26 to the plasma membrane and gap junction biogenesis we expressed ...

journal_title：Journal of cell science

authors： Thomas T,Jordan K,Simek J,Shao Q,Jedeszko C,Walton P,Laird DW

更新日期：2005-10-01 00:00:00

abstract：:During the interphase to metaphase transition, microtubules are destabilized by a cdc2 kinase-dependent phosphorylation event. This destabilization is due to a dramatic increase in the rate at which each growing microtubule starts to shrink (catastrophe rate). In principle, this could be brought about by lowering the ...

journal_title：Journal of cell science

authors： Faruki S,Dorée M,Karsenti E

更新日期：1992-01-01 00:00:00

abstract：:In diverse epithelia, cytoskeletal keratin intermediate filaments (IFs) associated with the cytoplasmic face of intercellular junctional desmosomes. The processes underlying desmosome formation and keratin IF interactions remain unclear. We have examined F9 embryonal carcinoma (EC) cell differentiation as a model for ...

journal_title：Journal of cell science

authors： Trevor KT,Steben LS

更新日期：1992-09-01 00:00:00

abstract：:Tight junctions between endothelial cells of brain capillaries are the most important structural elements of the blood-brain barrier. Cultured brain endothelial cells are known to loose tight junction-dependent blood-brain barrier characteristics such as macromolecular impermeability and high electrical resistance. We...

journal_title：Journal of cell science

authors： Wolburg H,Neuhaus J,Kniesel U,Krauss B,Schmid EM,Ocalan M,Farrell C,Risau W

更新日期：1994-05-01 00:00:00

abstract：:Mechanical stimulation affects many biological aspects in living cells through mechanotransduction. In myogenic precursor cells (MPCs), mechanical stimulation activates p38 mitogen-activated protein kinase (MAPK), a key regulator of myogenesis, via activating TNFα-converting enzyme (TACE, also known as ADAM17), to rel...

journal_title：Journal of cell science

authors： Niu A,Wen Y,Liu H,Zhan M,Jin B,Li YP

更新日期：2013-10-01 00:00:00

abstract：:Drosophila neural stem cells or neuroblasts undergo typical asymmetric cell division. An evolutionally conserved protein complex, comprising atypical protein kinase C (aPKC), Bazooka (Par-3) and Par-6, organizes cell polarity to direct these asymmetric divisions. Aurora-A (AurA) is a key molecule that links the divisi...

journal_title：Journal of cell science

authors： Ogawa H,Ohta N,Moon W,Matsuzaki F

更新日期：2009-09-15 00:00:00

abstract：:Increases in reactive oxygen species (ROS) have been implicated in age-related diseases, including cancer. The serine/threonine kinase protein kinase D1 (PKD1) is a stress-responsive kinase and sensor for reactive oxygen species, which can initiate cell survival through NF-kappaB signaling. We have previously shown th...

journal_title：Journal of cell science

authors： Cowell CF,Döppler H,Yan IK,Hausser A,Umezawa Y,Storz P

更新日期：2009-04-01 00:00:00

abstract：:Two of the best understood somatic cell mRNA cytoplasmic trafficking elements are those governing localization of beta-actin and myelin basic protein mRNAs. These cis-acting elements bind the trans-acting factors fibroblast ZBP-1 and hnRNP A2, respectively. It is not known whether these elements fulfil other roles in ...

journal_title：Journal of cell science

authors： Snee M,Kidd GJ,Munro TP,Smith R

更新日期：2002-12-01 00:00:00

abstract：:Anionic phospholipids PI(4,5)P2 and phosphatidylserine (PS) are enriched in the cytosolic leaflet of the plasma membrane where endocytic sites form. In this study, we investigated the roles of PI(4,5)P2 and PS in clathrin-mediated endocytosis (CME) site initiation and vesicle formation in Saccharomyces cerevisiae. Liv...

journal_title：Journal of cell science

authors： Sun Y,Drubin DG

更新日期：2012-12-15 00:00:00

abstract：:Patients with myotonic dystrophy type 1 exhibit a diversity of symptoms that affect many different organs. Among these are cognitive dysfunctions, the origin of which has remained elusive, partly because of the difficulty in accessing neural cells. Here, we have taken advantage of pluripotent stem cell lines derived f...

journal_title：Journal of cell science

authors： Denis JA,Gauthier M,Rachdi L,Aubert S,Giraud-Triboult K,Poydenot P,Benchoua A,Champon B,Maury Y,Baldeschi C,Scharfmann R,Piétu G,Peschanski M,Martinat C

更新日期：2013-04-15 00:00:00

abstract：:The tumor suppressor p53 is activated in response to many forms of cellular stress leading to cell cycle arrest, senescence or apoptosis. Appropriate sub-cellular localization is essential for modulating p53 function. We recently showed that p53 localizes to the nucleolus after proteasome inhibition with MG132 and thi...

journal_title：Journal of cell science

authors： Karni-Schmidt O,Zupnick A,Castillo M,Ahmed A,Matos T,Bouvet P,Cordon-Cardo C,Prives C

更新日期：2008-12-15 00:00:00

abstract：:An electron microscopic investigation of the events associated with meiosis in Lilium has revealed a number of changes in both the organellar population and the other cytoplasmic components. Ribosome numbers decrease significantly in early prophase and are later replenished in the tetrads, a process most likely involv...

journal_title：Journal of cell science

authors： Bird J,Porter EK,Dickinson HG

更新日期：1983-01-01 00:00:00

abstract：:Single cell clones were isolated from the human teratoma line, Tera-2. The cells of three of these clones were studied. The progressively growing cells were shown to be tumorigenic, and they were characterized by the lack of expression of beta 2-microglobulin and HLA-A,B,C determinants on the cell surface. The majorit...

journal_title：Journal of cell science

authors： Thompson S,Stern PL,Webb M,Walsh FS,Engstrom W,Evans EP,Shi WK,Hopkins B,Graham CF

更新日期：1984-12-01 00:00:00

abstract：:Mitochondrial homeostasis reflects a dynamic balance between membrane fission and fusion events thought essential for mitochondrial function. We report here that altered expression of the C. elegans BCL2 homolog CED-9 affects both mitochondrial fission and fusion. Although striated muscle cells lacking CED-9 have no a...

journal_title：Journal of cell science

authors： Tan FJ,Husain M,Manlandro CM,Koppenol M,Fire AZ,Hill RB

更新日期：2008-10-15 00:00:00

abstract：:Using a novel PCR approach, we have cloned a cDNA encoding the entire high molecular weight tau molecule from rat dorsal root ganglia. The resulting 2080 bp cDNA differs from low molecular weight rat brain tau by the insertion of a novel 762 bp region (exon 4a) between exons 4 and 5. This cDNA clone is identical in se...

journal_title：Journal of cell science

authors： Georgieff IS,Liem RK,Couchie D,Mavilia C,Nunez J,Shelanski ML

更新日期：1993-07-01 00:00:00

abstract：:During cell division, misaligned chromosomes are captured and aligned by motors before their segregation. The CENP-E motor is recruited to polar unattached kinetochores to facilitate chromosome alignment. The spindle checkpoint protein BubR1 (also known as BUB1B) has been reported as a CENP-E interacting partner, but ...

journal_title：Journal of cell science

authors： Legal T,Hayward D,Gluszek-Kustusz A,Blackburn EA,Spanos C,Rappsilber J,Gruneberg U,Welburn JPI

更新日期：2020-08-25 00:00:00

abstract：:During vertebrate gastrulation, a well-orchestrated series of morphogenetic changes leads to the formation of the three germ layers: the ectoderm, mesoderm and endoderm. The analysis of gene expression patterns during gastrulation has been central to the identification of genes involved in germ layer formation. Howeve...

journal_title：Journal of cell science

authors： Link V,Carvalho L,Castanon I,Stockinger P,Shevchenko A,Heisenberg CP

更新日期：2006-05-15 00:00:00

abstract：:HEK293 cells expressing wild-type CXCR2 recruit PH-Akt-GFP to the leading edge of the cell in response to chemokine. However, in cells expressing mutant CXCR2 defective in AP-2 and HIP binding, i.e. with a mutation in the LLKIL motif, PH-Akt-GFP does not localize to the leading edge in response to ligand. Inhibition o...

journal_title：Journal of cell science

authors： Sai J,Fan GH,Wang D,Richmond A

更新日期：2004-11-01 00:00:00

abstract：:The dynamics of cell adhesion sites control cell morphology and motility. Adhesion-site turnover is thought to depend on the local availability of the acidic phospholipid phosphatidylinositol-4,5-bisphosphate (PIP(2)). PIP(2) can bind to many cell adhesion proteins such as vinculin and talin, but the consequences of t...

journal_title：Journal of cell science

authors： Chandrasekar I,Stradal TE,Holt MR,Entschladen F,Jockusch BM,Ziegler WH

更新日期：2005-04-01 00:00:00

abstract：:Human SW-13 cells express the intermediate filament protein vimentin in a mosaic pattern (Hedberg, K. K. and Chen, L. B. (1986). Exp. Cell Res. 163, 509-517). We have isolated SW-13 clones that do (vim+) or do not (vim-) synthesize vimentin as analyzed using anti-intermediate filament immunofluorescence, electron micr...

journal_title：Journal of cell science

authors： Sarria AJ,Lieber JG,Nordeen SK,Evans RM

更新日期：1994-06-01 00:00:00

abstract：:The morphogenesis-related NDR kinase (MOR) pathway regulates morphogenesis in fungi. In spite of the high conservation of its components, impairing their functions results in highly divergent cellular responses depending on the fungal species. The reasons for such differences are unclear. Here we propose that the spec...

journal_title：Journal of cell science

authors： Sartorel E,Pérez-Martín J

更新日期：2012-10-01 00:00:00

abstract：:The iron-storage protein ferritin was found to be associated with highly purified coated vesicles (CV) from chicken liver. Chicken liver ferritin was morphologically similar to ferritin from horse spleen and could be isolated using a specific anti-ferritin monoclonal antibody. This antibody recognized a 240 X 10(3) Mr...

journal_title：Journal of cell science

authors： Passaniti A,Roth TF

更新日期：1989-02-01 00:00:00

abstract：:The mechanical implementation of phagocytosis requires a well-coordinated deployment of cytoplasm and membrane during the creation of a phagosome. We follow the time course of this process in initially round passive neutrophils presented with antibody-coated beads of radii 1.1 to 5.5 microm. In particular, we monitor ...

journal_title：Journal of cell science

authors： Herant M,Heinrich V,Dembo M

更新日期：2005-05-01 00:00:00

abstract：:Protein-protein interaction is often investigated using quantitative molecular microscopy with Förster resonant energy transfer (FRET). Here, we combined 'linear unmixing FRET' (lux-FRET) with the simultaneous application of a FRET-based biosensor for cAMP to investigate the oligomerization between the 5-HT7 receptor ...

journal_title：Journal of cell science

authors： Prasad S,Ponimaskin E,Zeug A

更新日期：2019-08-23 00:00:00