Abstract:
:Mechanical stimulation affects many biological aspects in living cells through mechanotransduction. In myogenic precursor cells (MPCs), mechanical stimulation activates p38 mitogen-activated protein kinase (MAPK), a key regulator of myogenesis, via activating TNFα-converting enzyme (TACE, also known as ADAM17), to release autocrine TNFα. However, the signaling mechanism of mechanical activation of TACE is unknown. Because TACE possesses the structural features of substrates of the non-receptor tyrosine kinase Src, we tested the hypothesis that Src mediates mechanical activation of TACE in MPCs. We observed that mechanical stretch of C2C12 or primary rat myoblasts rapidly activates Src, which in turn interacts and colocalizes with TACE, resulting in tyrosine phosphorylation and activation of TACE. Particularly, Src activates TACE via the phosphorylation of amino acid residue Tyr702 in the intracellular tail of TACE, resulting in increased TNFα release and p38 activation. Src inhibition or deficiency blocks stretch activation of the TACE-p38-MAPK signaling, resulting in impaired myogenic gene expression. In response to functional overloading, Src and TACE are activated in mouse soleus muscle. Further, overloading-induced myogenesis and regeneration are impaired in the soleus of Src(+/-) mice. Therefore, Src mediates mechano-activation of TACE and myogenesis.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Niu A,Wen Y,Liu H,Zhan M,Jin B,Li YPdoi
10.1242/jcs.125328subject
Has Abstractpub_date
2013-10-01 00:00:00pages
4349-57issue
Pt 19eissn
0021-9533issn
1477-9137pii
jcs.125328journal_volume
126pub_type
杂志文章abstract::We reported previously that Ca2+ depletion of Dictyostelium discoideum cells severely inhibits extracellular cyclic nucleotide phosphodiesterase (PD) synthesis at a post-transcriptional step. In this study, further experiments were performed to learn more about the nature of this phenomenon. Examination of the polysom...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
abstract::Thrombospondin-1 (TSP-1) and the highly related protein thrombospondin-2 (TSP-2) are trimeric extracellular molecules that have complex roles in wound healing, angiogenesis and matrix organisation. At the cellular level, TSP-1 supports cell adhesion and migration by the organisation of fascin spike cytoskeletal struct...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-06-01 00:00:00
abstract::Hepatocyte growth factor (HGF)/scatter factor modulates the motility of HT29 colon carcinoma cells in vitro by inducing morphological changes that depend on the type of extra-cellular matrix (ECM) ligand; HGF-induced scattering of HT29 cells is observed if cells are grown on plastic coated with serum proteins but not ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract::alpha3beta1-integrin is abundantly expressed in the epidermis, and in mice, ablation of the alpha3 gene results in embryonic defects and perinatal lethality. To determine the role of alpha3-integrin in adult skin development, we grafted skin from newborn alpha3-integrin-deficient mice on to ICRF nu/nu recipients. We r...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00475
更新日期:2003-07-01 00:00:00
abstract::The study of plasma membrane and phagosome membrane of Dictyostelium discoideum was performed using 2 lectins: concanavalin A (Con A) and Wheat Germ Agglutinin (WGA), and 2 markers of anionic sites: colloidal iron hydroxide (CIH) and cationized ferritin (CF). These labellings were applied to fixed partially broken cel...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1980-02-01 00:00:00
abstract::We have isolated suppressors of the deletion allele of delta-tubulin, uni3-1, in the biflagellate green alga Chlamydomonas reinhardtii. The deletion of delta-tubulin produces cells that assemble zero, one or two flagella and have basal bodies composed primarily of doublet rather than triplet microtubules. Flagellar nu...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00859
更新日期:2004-01-15 00:00:00
abstract::Unconventional myosin VIIA (Myo7a) is an actin-based motor molecule that normally functions in the cochlear hair cells of the inner ear. Mutations of MYO7A/Myo7a have been implicated in inherited deafness in both humans and mice. However, there is limited information about the functions of Myo7a outside of the special...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.209924
更新日期:2018-02-22 00:00:00
abstract::Asymmetric dimethylarginine (ADMA) is an inhibitor of nitric oxide production associated with abnormal blood vessel growth and repair, however, the mechanism of action of ADMA is not well understood. We studied the role of exogenous and endogenous ADMA in the regulation of cell motility and actin cytoskeleton in porci...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.002212
更新日期:2007-03-15 00:00:00
abstract::The appropriate targeting of membrane-associated proteins involves a diversity of motifs including post-translational modifications and specific protein sequences. Phosphoproteins of the stathmin family are important regulators of microtubule dynamics, in particular in the developing and mature nervous system. Whereas...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02349
更新日期:2005-05-15 00:00:00
abstract::The mechanical implementation of phagocytosis requires a well-coordinated deployment of cytoplasm and membrane during the creation of a phagosome. We follow the time course of this process in initially round passive neutrophils presented with antibody-coated beads of radii 1.1 to 5.5 microm. In particular, we monitor ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02275
更新日期:2005-05-01 00:00:00
abstract::Cilia and flagella are conserved eukaryotic organelles important for motility and sensory. The RanGTPase, best known for nucleocytoplasmic transport functions, may also play a role in protein trafficking into the specialized flagellar/ciliary compartments, although the regulatory mechanisms controlling Ran activity at...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.148015
更新日期:2014-11-15 00:00:00
abstract::We have previously shown that chymotrypsin-cleaved soluble uPAR (D2D3(88-274)) elicits migration of monocytic cells through interaction with FPRL-1, a G protein-coupled receptor that is homologous to the fMLP receptor. Here, we report that D2D3(88-274) also modulates the ability of monocytes to migrate in response to ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01149
更新日期:2004-06-15 00:00:00
abstract::Voltage-gated Ca2+ (CaV) channels couple membrane depolarization to Ca2+ influx, triggering a range of Ca2+-dependent cellular processes. CaV channels are, therefore, crucial in shaping neuronal activity and function, depending on their individual temporal and spatial properties. Furthermore, many neurotransmitters an...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.207886
更新日期:2017-11-15 00:00:00
abstract::We have investigated the role in macrophage lysosomal enzyme release of arachidonate metabolites, extracellular divalent cations and cyclic AMP (cAMP) which modulate secretion in other cell types. Lysosomal enzyme secretion induced by zymosan was accompanied by release of malondialdehyde (MDA), which is derived from a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::Members of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family of proteolytic enzymes are implicated in a variety of physiological processes, such as collagen maturation, organogenesis, angiogenesis, reproduction and inflammation. Moreover, deficiency or overexpression of certain ADAMTS ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.005751
更新日期:2007-10-15 00:00:00
abstract::Accumulation of adipocytes and collagen type-I-producing cells (fibrosis) is observed in muscular dystrophies. The origin of these cells had been largely unknown, but recently we identified mesenchymal progenitors positive for platelet-derived growth factor receptor alpha (PDGFRα) as the origin of adipocytes in skelet...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.086629
更新日期:2011-11-01 00:00:00
abstract::Intracellular membrane fusion occurs with exquisite coordination and specificity. Each fusion event requires three basic components: Rab-GTPases organize the fusion site; SNARE proteins act during fusion; and N-ethylmaleimide-sensitive factor (NSF) plus its cofactor alpha-SNAP are required for recycling or activation ...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.02561
更新日期:2005-09-01 00:00:00
abstract::In this study we describe DNA-RNA complexes in matrix DNA of Friend cells. The presence of such unusual structures is confirmed by the following evidence. When a preparation of matrix DNA is electrophoresed in agarose an RNA component always migrates together with DNA. There should be a close interaction between DNA a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00
abstract::Mesenchyme fragments from early wing buds of normal and talpid3 mutant chick embryos were explanted for culture in plastic Petri dishes and the behaviour of individual cells as they moved out on to the plastic surface was studied by time-lapse ciné photography, followed by statistical analysis. Two parameters of cell ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1975-07-01 00:00:00
abstract::The protein kinase inhibitor H-7 prevents the assembly of tight junctions in cultured Madin Darby Canine Kidney (MDCK) epithelial cells (Balda et al. (1991) J. Membr. Biol. 122, 193-202; Nigam et al. (1991) Biochem. Biophys. Res. Commun. 181, 548-553); however, its mechanism of action is unknown. To understand the bas...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract::Dynamin, a microtubule-activated GTPase, has recently been identified as the product of the shibire gene in Drosophila. shi(ts) mutants are defective in synaptic vesicle recycling, which leads to rapid and reversible temperature-sensitive paralysis. In the present study, results from RNase protection assays and analys...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00
abstract::We have recently shown that leukotriene D(4) (LTD(4)) increases cell survival in intestinal epithelial cells. Here we report and explore the complementary finding that LTD(4) also enhances proliferation in these cells. This proliferative response was approximately half of that induced by epidermal growth factor (EGF) ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-05-01 00:00:00
abstract::Male infertility has become a worldwide health problem, but the etiologies of most cases are still unknown. SEPT12, a GTP-binding protein, is involved in male fertility. Two SEPT12 mutations (SEPT12(T89M) and SEPT12(D197N)) have been identified in infertile men who have a defective sperm annulus with a bent tail. The ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.158998
更新日期:2015-03-01 00:00:00
abstract::The events responsible for repair of DNA interstrand cross-links in mammalian cells, the proteins involved and their interactions with each other are poorly understood. The present study demonstrates that the structural protein nonerythroid alpha spectrin (alphaSpIISigma*), present in normal human cell nuclei, plays a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00294
更新日期:2003-03-01 00:00:00
abstract::Low-intensity pulsed ultrasound (LIPUS) is a therapy used clinically to promote healing. Using live-cell imaging we show that LIPUS stimulation, acting through integrin-mediated cell-matrix adhesions, rapidly induces Rac1 activation associated with dramatic actin cytoskeleton rearrangements. Our study demonstrates tha...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.192781
更新日期:2017-07-15 00:00:00
abstract::Previously we have shown that the Saccharomyces cerevisiae CCZ1 (YBR131w) gene encodes a protein involved in protein trafficking. Deletion of this gene leads to fragmentation of the vacuole typical of the class B vps mutants. Genetic and biochemical data indicated that Ccz1p is required for fusion of various transport...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract::We have found that Ehrlich ascites tumour (EAT) cells, deprived of any carbon source, and suspended at a density of 2 X 10(5) cells/cm3, begin to die only after 12 h of starvation, though it is known that under these conditions they lose over 80% of their ATP within 30 min. Moreover, we have found that the viability o...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1985-08-01 00:00:00
abstract::MOR1, the Arabidopsis thaliana homologue of the Xenopus microtubule-associated protein MAP215, is required for spatial organization of the acentrosomal microtubule arrays of plant cells. To determine how loss of MOR1 function affects microtubule dynamics, we compared various parameters of microtubule dynamics in the t...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.039065
更新日期:2008-12-15 00:00:00
abstract::Natural killer (NK) cells eliminate abnormal cells through the release of cytolytic granule contents. In this process, NK cells must adhere to target cells through integrin-mediated adhesion, which is highly dependent on the generation of F-actin. Ena/VASP-like (EVL) is an actin regulatory protein previously shown to ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.230508
更新日期:2019-07-18 00:00:00
abstract::We have previously shown that Xenopus oocytes, eggs, and early embryos contain lamins LII and LIII, and that portions of each are associated with distinct egg vesicle populations. We now report that a lamin similar or identical to the B-type lamin LI is also present in oocyte nuclei and in egg extracts. We have quanti...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00