NKG2D-DAP10 signaling recruits EVL to the cytotoxic synapse to generate F-actin and promote NK cell cytotoxicity.

abstract：:To enhance our understanding of the function(s) of gamma-tubulin-complex proteins (GCPs), we identified and analyzed the functions of the Aspergillus nidulans homologs of GCP2-GCP6 (here designated GCPB-GCBF). The gamma-tubulin small complex (gamma-TuSC) components, gamma-tubulin, GCPB and GCPC, are essential for viab...

journal_title：Journal of cell science

authors： Xiong Y,Oakley BR

更新日期：2009-11-15 00:00:00

abstract：:Poly(ADP-ribose) polymerase-1 (PARP1) is a nuclear enzyme that can trigger caspase-independent necrosis. Two main mechanisms for this have been proposed: one involving RIP1 and JNK kinases and mitochondrial permeability transition (MPT), the other involving calpain-mediated activation of Bax and mitochondrial release ...

journal_title：Journal of cell science

authors： Douglas DL,Baines CP

更新日期：2014-10-01 00:00:00

abstract：:When migrating mesenchymal cells collide, they exhibit a 'contact inhibition of locomotion' response that results in reversal of their front-rear polarity by extension of a new leading edge, which enables their migration away from the opposing contacted cell. The critical cytoskeletal rearrangements underpinning these...

journal_title：Journal of cell science

authors： Kadir S,Astin JW,Tahtamouni L,Martin P,Nobes CD

更新日期：2011-08-01 00:00:00

abstract：:The effects of hyaluronate on the morphology, motile and social behaviour of embryonic chick heart fibroblasts were examined in the presence or absence of hyaluronate binding proteins (HABP). HABP were inserted onto fibroblasts after treating cells with 0.2 M-urea. Such treatment increased the binding of [125I]HABP to...

journal_title：Journal of cell science

authors： Turley EA,Bowman P,Kytryk MA

更新日期：1985-10-01 00:00:00

abstract：:Microscopic green algae inhabiting desert microbiotic crusts are remarkably diverse phylogenetically, and many desert lineages have independently evolved from aquatic ancestors. Here we worked with five desert and aquatic species within the family Scenedesmaceae to examine mechanisms that underlie desiccation toleranc...

journal_title：Journal of cell science

authors： Cardon ZG,Peredo EL,Dohnalkova AC,Gershone HL,Bezanilla M

更新日期：2018-04-10 00:00:00

abstract：:To examine the internal structure of chromatin fibers, we have developed procedures for partial denaturation of small chromatin fragments (8-30 nucleosomes) from chicken erythrocytes. Electron micrographs of samples prepared under conditions that cause nucleosome dissociation show rods and loops projecting from short ...

journal_title：Journal of cell science

authors： Bermúdez A,Bartolomé S,Daban JR

更新日期：1998-06-01 00:00:00

abstract：:The Arp2/3 complex is important for morphogenesis in various developmental systems, but specific in vivo roles for this complex in cells that move during morphogenesis are not well understood. We have examined cellular roles for Arp2/3 in the Caenorhabditis elegans embryo. In C. elegans, the first morphogenetic moveme...

journal_title：Journal of cell science

authors： Roh-Johnson M,Goldstein B

更新日期：2009-11-01 00:00:00

abstract：:Although the anti-apoptotic activity of Bcl-2 has been extensively studied, its mode of action is still incompletely understood. In the nematode Caenorhabditis elegans, 131 of 1090 somatic cells undergo programmed cell death during development. Transgenic expression of human Bcl-2 reduced cell death during nematode de...

journal_title：Journal of cell science

authors： Jabbour AM,Puryer MA,Yu JY,Lithgow T,Riffkin CD,Ashley DM,Vaux DL,Ekert PG,Hawkins CJ

更新日期：2006-06-15 00:00:00

abstract：:The position of the nucleus is regulated in different developmental stages and cellular events. During polarization, the nucleus moves away from the future leading edge and this movement is required for proper cell migration. Nuclear movement requires the LINC complex components nesprin-2G and SUN2, which form transme...

journal_title：Journal of cell science

authors： Borrego-Pinto J,Jegou T,Osorio DS,Auradé F,Gorjánácz M,Koch B,Mattaj IW,Gomes ER

更新日期：2012-03-01 00:00:00

abstract：:The adaptive immune response depends on the interaction of T cells and antigen-presenting cells at the immune synapse. Formation of the immune synapse and the subsequent T-cell activation are highly dependent on the actin cytoskeleton. In this work, we describe that T cells express drebrin, a neuronal actin-binding pr...

journal_title：Journal of cell science

authors： Pérez-Martínez M,Gordón-Alonso M,Cabrero JR,Barrero-Villar M,Rey M,Mittelbrunn M,Lamana A,Morlino G,Calabia C,Yamazaki H,Shirao T,Vázquez J,González-Amaro R,Veiga E,Sánchez-Madrid F

更新日期：2010-04-01 00:00:00

abstract：:Oocytes of Xenopus laevis in pachytene and early diplotene of meiosis have been studied using the Miller spreading technique. Transcription first appears in germinal vesicles 25-40 micrometers in diameter, when the oocyte is in early diplotene. Transcription at this stage consists of arrays of short RNP transcripts, i...

journal_title：Journal of cell science

authors： Hill RS,Macgregor HC

更新日期：1980-08-01 00:00:00

abstract：:Biglycan and decorin are small chondroitin/dermatan sulphate proteoglycans in the extracellular matrix of connective tissue that belong to the family of structurally related proteoglycans called small leucine-rich repeat proteins. We show for the first time that biglycan and decorin induce morphological and cytoskelet...

journal_title：Journal of cell science

authors： Tufvesson E,Westergren-Thorsson G

更新日期：2003-12-01 00:00:00

abstract：:The detergent-insoluble T. brucei cytoskeleton consists of several morphologically distinct regions and organelles, many of which are detectable only by electron microscopy. We have produced a set of monoclonal antibodies that define each structural component of this highly ordered cytoskeleton. The monoclonal antibod...

journal_title：Journal of cell science

authors： Woods A,Sherwin T,Sasse R,MacRae TH,Baines AJ,Gull K

更新日期：1989-07-01 00:00:00

abstract：:The family of integrin transmembrane receptors is essential for the normal function of multicellular organisms by facilitating cell-extracellular matrix adhesion. The vitronectin-binding integrin αVβ5 localizes to focal adhesions (FAs) as well as poorly characterized flat clathrin lattices (FCLs). Here, we show that, ...

journal_title：Journal of cell science

authors： Zuidema A,Wang W,Kreft M,Te Molder L,Hoekman L,Bleijerveld OB,Nahidiazar L,Janssen H,Sonnenberg A

更新日期：2018-11-05 00:00:00

abstract：:Promyelocytic leukemia nuclear bodies (PML-NBs) are mobile subnuclear organelles formed by PML and Sp100 protein. They have been reported to have a role in transcription, DNA replication and repair, telomere lengthening, cell cycle control and tumor suppression. We have conducted high-resolution 4Pi fluorescence laser...

journal_title：Journal of cell science

authors： Lang M,Jegou T,Chung I,Richter K,Münch S,Udvarhelyi A,Cremer C,Hemmerich P,Engelhardt J,Hell SW,Rippe K

更新日期：2010-02-01 00:00:00

abstract：:To clarify the involvement of actin in the formation of the yeast cell wall, reverting protoplasts of Schizosaccharomyces pombe were used as a simple model system. Actin of reverting protoplasts was labeled with rhodamine-conjugated phalloidin and observed by conventional fluorescence microscopy and laser scanning con...

journal_title：Journal of cell science

authors： Kobori H,Yamada N,Taki A,Osumi M

更新日期：1989-12-01 00:00:00

abstract：:The large GTPase dynamin, long known for its role in endocytosis, has most recently been implicated as a facilitator of cell migration and invasion. Recent observations link dynamin to the cycle of membrane expansion and retraction essential for cell motility. Its role in actin polymerization, membrane deformation and...

journal_title：Journal of cell science

authors： Kruchten AE,McNiven MA

更新日期：2006-05-01 00:00:00

abstract：:Megalin is a receptor expressed by embryonic epithelia that mediates endocytosis of numerous ligands, including sonic hedgehog (Shh) and retinol, the precursor to retinoic acid (RA). The importance of Shh and RA signaling in neurodevelopment, combined with the fact that megalin-deficient mice show profound neurodevelo...

journal_title：Journal of cell science

authors： McCarthy RA,Argraves WS

更新日期：2003-03-15 00:00:00

abstract：:Activity of AURKA is controlled through multiple mechanisms including phosphorylation, ubiquitin-mediated degradation and allosteric interaction with TPX2. Activity peaks at mitosis, before AURKA is degraded during and after mitotic exit in a process strictly dependent on the APC/C coactivator FZR1. We used FZR1 knock...

journal_title：Journal of cell science

authors： Abdelbaki A,Akman HB,Poteau M,Grant R,Gavet O,Guarguaglini G,Lindon C

更新日期：2020-06-16 00:00:00

abstract：:Autosomal dominant polycystic kidney disease (ADPKD) is the most common monogenic human disease, with around 12.5 million people affected worldwide. ADPKD results from mutations in either PKD1 or PKD2, which encode the atypical G-protein coupled receptor polycystin-1 (PC1) and the transient receptor potential channel ...

journal_title：Journal of cell science

authors： Tilley FC,Gallon M,Luo C,Danson CM,Zhou J,Cullen PJ

更新日期：2018-06-06 00:00:00

abstract：:Polyethylene glycol (PEG) was used to fuse trout sperm with Chinese hamster fibroblasts (CHW-1102). As judged by light microscopy, PEG significantly increased the number of associations between the two cell types. Electron microscopy revealed that the sperm nuclei were in the cytoplasm of CHW-1102 in cultures that had...

journal_title：Journal of cell science

authors： Yip JH,Bols NC

更新日期：1982-02-01 00:00:00

abstract：:Adhesion between cells is established by the formation of specialized intercellular junctional complexes, such as desmosomes. Desmosomes contain isoforms of two members of the cadherin superfamily of cell adhesion proteins, desmocollins (Dsc) and desmogleins (Dsg), but their combinatorial roles in desmosome assembly a...

journal_title：Journal of cell science

authors： Lowndes M,Rakshit S,Shafraz O,Borghi N,Harmon RM,Green KJ,Sivasankar S,Nelson WJ

更新日期：2014-05-15 00:00:00

abstract：:Super-resolution fluorescence imaging by single-molecule photoactivation or photoswitching and position determination (localization microscopy) has the potential to fundamentally revolutionize our understanding of how cellular function is encoded at the molecular level. Among all powerful, high-resolution imaging tech...

journal_title：Journal of cell science

authors： Sauer M

更新日期：2013-08-15 00:00:00

abstract：:In S. cerevisiae, spindle orientation is linked to the inheritance of the ;old' spindle pole by the bud. A player in this asymmetric commitment, Bud6p, promotes cortical capture of astral microtubules. Additionally, Bud6p stimulates actin cable formation though the formin Bni1p. A relationship with the second formin, ...

journal_title：Journal of cell science

authors： Delgehyr N,Lopes CS,Moir CA,Huisman SM,Segal M

更新日期：2008-11-15 00:00:00

abstract：:CENP-B binds to CENP-B boxes on centromeric satellite DNAs (known as alphoid DNA in humans). CENP-B maintains kinetochore function through interactions with CENP-A nucleosomes and CENP-C. CENP-B binding to transfected alphoid DNA can induce de novo CENP-A assembly, functional centromere and kinetochore formation, and ...

journal_title：Journal of cell science

authors： Otake K,Ohzeki JI,Shono N,Kugou K,Okazaki K,Nagase T,Yamakawa H,Kouprina N,Larionov V,Kimura H,Earnshaw WC,Masumoto H

更新日期：2020-08-11 00:00:00

abstract：:In response to endovascular injury, platelet-derived growth factor-BB (PDGF-BB) and basic fibroblast growth factor (bFGF) are released locally and modulate vascular smooth muscle cells (SMC) proliferation and migration within the vascular wall. The aim of the present in vitro study was to determine how rat aorta SMC r...

journal_title：Journal of cell science

authors： Facchiano A,De Marchis F,Turchetti E,Facchiano F,Guglielmi M,Denaro A,Palumbo R,Scoccianti M,Capogrossi MC

更新日期：2000-08-01 00:00:00

abstract：:The study of plasma membrane and phagosome membrane of Dictyostelium discoideum was performed using 2 lectins: concanavalin A (Con A) and Wheat Germ Agglutinin (WGA), and 2 markers of anionic sites: colloidal iron hydroxide (CIH) and cationized ferritin (CF). These labellings were applied to fixed partially broken cel...

journal_title：Journal of cell science

authors： Ryter A,Hellio R

更新日期：1980-02-01 00:00:00

abstract：:Iron (Fe(2+), Fe(3+)) homeostasis is a tightly regulated process, involving precise control of iron influx and egress from cells. Although the mechanisms of its import into cells by iron carrier molecules are well characterized, iron export remains poorly understood. The current paradigm envisages unique functions ass...

journal_title：Journal of cell science

authors： Sheokand N,Malhotra H,Kumar S,Tillu VA,Chauhan AS,Raje CI,Raje M

更新日期：2014-10-01 00:00:00

abstract：:The median cell cycle of synchronized NHIK3025 cells grown in Eagle's MEM with 10% foetal calf serum was 23.6 h, compared to 18 h observed earlier in Puck's E2a medium with 30% serum (20% human and 10% horse). This difference is due to prolongation of both G1 and G2 in the MEM type medium. Testosterone and 4-androsten...

journal_title：Journal of cell science

authors： Ostgaard K,Wibe E,Lindmo T,Eik-Nes KB

更新日期：1981-04-01 00:00:00

abstract：:We investigated the role of Pav-KLP, a kinesin-6, in the coordination of spindle and cortical dynamics during mitosis in Drosophila embryos. In vitro, Pav-KLP behaves as a dimer. In vivo, it localizes to mitotic spindles and furrows. Inhibition of Pav-KLP causes defects in both spindle dynamics and furrow ingression, ...

journal_title：Journal of cell science

authors： Sommi P,Ananthakrishnan R,Cheerambathur DK,Kwon M,Morales-Mulia S,Brust-Mascher I,Mogilner A

更新日期：2010-06-01 00:00:00