Abstract:
:The detergent-insoluble T. brucei cytoskeleton consists of several morphologically distinct regions and organelles, many of which are detectable only by electron microscopy. We have produced a set of monoclonal antibodies that define each structural component of this highly ordered cytoskeleton. The monoclonal antibodies were selected by cloning of hybridomas produced from mice injected with complex mixtures of proteins of either the cytoskeleton itself or salt extracts thereof. Four antibodies define particular tubulin isotypes and locate the microtubules of the axoneme and sub-pellicular array; two antibodies recognize the flagellum attachment zone; one recognizes the paraflagellar rod and another the basal bodies. Finally, one antibody defines a detergent-insoluble component of the nucleus. The antigens detected by each monoclonal antibody have been analysed by immunofluorescence microscopy, immunogold electron microscopy and Western blotting.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Woods A,Sherwin T,Sasse R,MacRae TH,Baines AJ,Gull Ksubject
Has Abstractpub_date
1989-07-01 00:00:00pages
491-500eissn
0021-9533issn
1477-9137journal_volume
93 ( Pt 3)pub_type
杂志文章abstract::Intracellular pH has been measured by laser microspectrofluorimetry, using the pH-sensitive dyes SNARF-1, SNARF-calcein and SNARF-1-dextran. By this technique it was possible to accurately determine pH in volumes as small as 0.5 x 0.5 x 1 microns 3. The probes were loaded into the cells either by diffusion of their ac...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
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journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
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更新日期:1978-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2006-01-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1986-06-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1980-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2009-07-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2006-12-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1990-05-01 00:00:00
abstract::Compartmentalization of surface membrane antigens into discrete regions or domains is a characteristic feature of differentiated cells. In mammalian spermatozoa at least 5 surface domains are known, implying the presence of barriers or boundaries within the plasma membrane. Using the technique of fluorescence recovery...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1997-05-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.213462
更新日期:2018-05-04 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2016-08-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.150888
更新日期:2014-07-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1990-11-01 00:00:00
abstract::The effects of hyaluronate on the morphology, motile and social behaviour of embryonic chick heart fibroblasts were examined in the presence or absence of hyaluronate binding proteins (HABP). HABP were inserted onto fibroblasts after treating cells with 0.2 M-urea. Such treatment increased the binding of [125I]HABP to...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1985-10-01 00:00:00
abstract::Nutritive tubes, the microtubule-based translocation channels that link the trophic tissue to the developing oocytes in the ovaries of hemipteran insects, have been isolated and examined using video-enhanced differential interference contrast microscopy. When viewed in this way the nutritive tubes are seen to fray int...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1987-12-01 00:00:00
abstract::An essential step in mammalian fertilisation is the sperm acrosome reaction (AR) - exocytosis of a single large vesicle (the acrosome) that surrounds the nucleus at the apical sperm head. The acrosomal and plasma membranes fuse, resulting in both the release of factors that might facilitate penetration of the zona pel...
journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-06-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2018-11-30 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2013-08-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Occludin and 18 distinct members of the claudin family are tetra-span transmembrane proteins that are localized in cell-specific tight junctions (TJs). A previous study showed that expression of chick occludin in Madin-Darby canine kidney (MDCK) cells raised transepithelial electrical resistance (TER) and, paradoxical...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-10-01 00:00:00
abstract::Ezrin/radixin/moesin (ERM) proteins are thought to play an important role in organizing cortical actin-based cytoskeletons through cross-linkage of actin filaments with integral membrane proteins. Recent in vitro biochemical studies have revealed that ERM proteins phosphorylated on their COOH-terminal threonine residu...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
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journal_title:Journal of cell science
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更新日期:2015-03-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1999-04-01 00:00:00