Abstract：
:Madin-Darby canine kidney cells (MDCK) were transfected with a cDNA encoding the glycosyl-phosphatidylinositol (GPI)-anchored protein mouse Thy-1 in order to study the steady-state surface distribution of exogenous and endogenous GPI-linked proteins. Immunofluorescence of transfected cells grown on collagen-coated coverslips showed that expression of Thy-1 was variable throughout the epithelium, with some cells expressing large amounts of Thy-1 adjacent to very faintly staining cells. Selective surface iodination of cells grown on collagen-coated or uncoated transwell filters followed by immunoprecipitation of Thy-1 demonstrated that all the Thy-1 was present exclusively in the apical plasma membrane. Although cells grown on uncoated filters had much smaller amounts of Thy-1, it was consistently localized on the apical surfaces. Immunofluorescent localization of Thy-1 on 1 micron frozen sections of filter-grown cells demonstrated that all the Thy-1 was on the apical surface and there was no detectable intracellular pool. Phosphatidylinositol-specific phospholipase C digestion of intact iodinated monolayers released Thy-1 only into the apical medium, indicating that Thy-1 was processed normally in transfected cells and was anchored by a GPI-tail. In agreement with previous findings, endogenous GPI-linked proteins were found only on the apical plasma membrane. These results suggest that there is a common mechanism for sorting and targeting of GPI-linked proteins in polarized epithelial cells.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Wilson JM,Fasel N,Kraehenbuhl JPsubject
Has Abstractpub_date
1990-05-01 00:00:00pages
143-9eissn
0021-9533issn
1477-9137journal_volume
96 ( Pt 1)pub_type
杂志文章abstract：:Understanding the interactions between distinct epithelial cells would help us to understand the development of tissues. Drosophila imaginal discs, which are made up of two types of epithelial cells, provide good model systems for such studies. The disc proper or the columnar epithelial cells are apposed to a layer of...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02464
更新日期：2005-08-01 00:00:00
abstract：:The Arabidopsis thaliana ASY1 gene is essential for homologous chromosome synapsis. Antibodies specific to Asy1 protein and its homologue BoAsy1 from the related crop species Brassica oleracea have been used to investigate the temporal expression and localization of the protein in both species. Asy1 is initially detec...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00048
更新日期：2002-09-15 00:00:00
abstract：:Friedreich's ataxia is a recessive neurodegenerative disease due to insufficient expression of the mitochondrial protein frataxin. Although it has been shown that frataxin is involved in the control of intracellular iron metabolism, by interfering with the mitochondrial biosynthesis of proteins with iron/sulphur (Fe/S...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02516
更新日期：2005-09-01 00:00:00
abstract：:Cells constantly adapt to various environmental changes and stresses. The way in which nutrient and stress levels in a cell feed back to control metabolism and growth are, unsurprisingly, extremely complex, as responding with great sensitivity and speed to the 'feast or famine, slack or stress' status of its environme...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.168922
更新日期：2015-06-15 00:00:00
abstract：:Protein kinases AKT and PKBR1 of Dictyostelium belong to the AGC protein kinase superfamily. AKT and PKBR1 are phosphorylated at similar sites by phosphoinositide-dependent kinase 1 (PDK1) and TORC2 kinases; however, they have different subcellular localizing domains. AKT has a phosphoinositide 3-kinase (PI3K)/phospha...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.064022
更新日期：2010-03-15 00:00:00
abstract：:This study examined the role of Ca(2+) in regulatory volume decrease by Necturus erythrocytes. Hypotonic shock (50% tonicity) stimulated an increase in cytosolic free Ca(2+), detected using epi-fluorescence microscopy and the fluorescent Ca(2+) indicator fluo-4-AM (10 microM). A similar increase in fluorescence did no...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00202
更新日期：2003-01-01 00:00:00
abstract：:Fatty acids constitute an important energy source for various tissues. The mechanisms that mediate and control uptake of free fatty acids from the circulation, however, are poorly understood. Here we show that efficient fatty-acid uptake by yeast cells requires the protein kinase Ypk1, the orthologue of the human seru...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.063073
更新日期：2010-07-01 00:00:00
abstract：:Although significant progress has recently been made in culturing mammalian urothelial cells, relatively little is known about their biochemical differentiation. In this paper, we assessed the differentiation state of cultured bovine urothelial cells by analyzing their keratins and a cell surface marker, uroplakin I. ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1990-11-01 00:00:00
abstract：:The migration of neutrophils from blood vessels to peripheral tissues is a key step of inflammation. This requires the formation of transient gaps between endothelial cells with concomitant leucocyte squeezing through these narrow apertures and immediate restoration of endothelium continuity. It is currently considere...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
abstract：:The vacuolar H(+)-ATPase (V-ATPase) is a multi-subunit enzyme that plays important roles in eukaryotic cells. In Dictyostelium, it is found primarily in membranes of the contractile vacuole complex, where it energizes fluid accumulation by this osmoregulatory organelle and also in membranes of endolysosomes, where it ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-07-15 00:00:00
abstract：:A temperature-sensitive mutant tsBN63 cell line was isolated by the fluorodeoxyuridine method from the BHK21/13 cell line after mutagenesis with nitrosoguanidine. When cultures of tsBN63 cells growing asynchronously at 33.5 degrees C were shifted to 39.5 degrees C, a nonpermissive temperature, the ability for protein ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-09-01 00:00:00
abstract：:The role of inhibition of prelamin A processing in the inhibition of DNA synthesis by lovastatin was examined by expressing prelamin A in F9 teratocarcinoma cells. These cells, normally lacking expression of the A/C lamins, were transfected with constructs expressing either prelamin A or mature lamin A and the effect ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-08-01 00:00:00
abstract：:The cell wall of 24-h zygotes of Fucus serratus is composed of 3 layers--an inner fibrillar layer (sulphated fucan), an outer fibrillar layer (alginic aicd/cellulose) and an exterior amorphous layer (sulphated fucan, alginic acid). The 2 layers containing sulphated fucan are preferentially thickened at the rhizoid pol...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1978-08-01 00:00:00
abstract：:Phagocytosis is an actin-based process used by macrophages to clear particles greater than 0.5 microm in diameter. In addition to its role in immunological responses, phagocytosis is also necessary for tissue remodeling and repair. To prevent catastrophic autoimmune reactions, phagocytosis must be tightly regulated. I...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-02-15 00:00:00
abstract：:Beta-arrestin 1 and beta-arrestin 2 are well-known negative regulators of G-protein-coupled receptor (GPCR) signaling. Upon GPCR activation, beta-arrestins translocate to the cell membrane and bind to the agonist-occupied receptors. This uncouples these receptors from G proteins and promotes their internalization, thu...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.03338
更新日期：2007-01-15 00:00:00
abstract：:Mutations in leucine-rich repeat kinase 2 (LRRK2) are associated with Parkinson’s disease, but the precise physiological function of the protein remains ill-defined. Recently, our group proposed a model in which LRRK2 kinase activity is part of an EndoA phosphorylation cycle that facilitates efficient vesicle formatio...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.158196
更新日期：2015-02-01 00:00:00
abstract：:Calcineurin-NFAT signaling has been shown to control activity-dependent muscle gene regulation and induce a program of gene expression typical of slow oxidative muscle fibers. Following Ca2+-calmodulin stimulation, calcineurin dephosphorylates NFAT proteins and induces their translocation into the nucleus. However, NF...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02875
更新日期：2006-04-15 00:00:00
abstract：:Successful implantation requires the synchronization of viable embryonic development with endometrial receptivity. The mechanisms allowing for the initiation of crosstalk between the embryo and the endometrium remain elusive; however, recent studies have revealed that there are alterations in endometrial microRNAs (mi...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.164004
更新日期：2015-02-15 00:00:00
abstract：:The COPII coat and the small GTPase Sar1 mediate protein export from the endoplasmic reticulum (ER) via specialized domains known as the ER exit sites. The peripheral ER protein Sec16 has been proposed to organize ER exit sites. However, it remains unclear how these molecules drive COPII coat polymerization. Here, we ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.203844
更新日期：2017-09-01 00:00:00
abstract：:The small GTPase Arf4 and the Arf GTPase-activating protein (GAP) ASAP1 cooperatively sequester sensory receptor cargo into transport carriers targeted to primary cilia, but the input that drives Arf4 activation in this process remains unknown. Here, we show, by using frog retinas and recombinant human proteins, that ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.205492
更新日期：2017-12-01 00:00:00
abstract：:The establishment of the junctional complex in epithelial cells requires the presence of extracellular calcium, and is controlled by a network of reactions involving G-proteins, phospholipase C and protein kinase C. Since potential candidates for phosphorylation are the tight junction associated proteins ZO1, ZO2 and ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-12-01 00:00:00
abstract：:The plasma membrane of cells is composed of lateral heterogeneities, patches and microdomains. These membrane microdomains or lipid rafts are enriched in glycosphingolipids and cholesterol and have been implicated in cellular processes such as membrane sorting and signal transduction. In this study we investigated the...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-06-15 00:00:00
abstract：:Sister chromatid cohesion is essential for proper segregation of the genome in mitosis and meiosis. Central to this process is cohesin, a multi-protein complex conserved from yeast to human. Previous genetic studies in fungi have identified Pds5/BimD/Spo76 as an additional factor implicated in cohesion. Here we descri...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02355
更新日期：2005-05-15 00:00:00
abstract：:The molecular chaperone Hsp90 has been found ubiquitously as a predominantly cytoplasmic dimer. By interacting with cytoplasmic or nuclear proteins such as pp60v-src or steroid receptors, Hsp90 helps its targets to become competent for full biological activity. Mutational deletion analysis of some properties of chicke...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:We have discovered a novel mitosis-specific human autoantigen that arises at the centromeres of prophase chromosomes, but ultimately participates in formation of an organelle that bisects the cell at late anaphase and during telophase. The organelle, discernible as a three-dimensional disc by confocal microscopy, enco...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-07-01 00:00:00
abstract：:Fusion of large and small karyoplasts produced from metaphase II mouse oocytes with interphase blastomeres from 2-cell and 8-cell embryos (volume ratio of partners, 1:1) results in premature chromosome condensation (PCC) of the interphase nucleus in the majority of the fusion products (hybrids). Fused under the same e...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1986-08-01 00:00:00
abstract：:3-Phosphoinositide-dependent protein kinase-1 (PDK1) and phospholipase C (PLC)γ1 are two key enzymes in signal transduction that control several intracellular processes. Despite the fact that PLCγ1 has been investigated for several years, the mechanisms of activation of this enzyme are still not completely clear. Simi...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.100511
更新日期：2012-07-01 00:00:00
abstract：:How ion channels localize and distribute on the cell membrane remains incompletely understood. We show that interventions that vary cell adhesion proteins and cell size also affect the membrane current density of inward-rectifier K+ channels (Kir2.1; encoded by KCNJ2) and profoundly alter the action potential shape of...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.225383
更新日期：2019-09-02 00:00:00
abstract：:In this paper, we identify the chondroitin sulfate proteoglycan versican V2 as a major inhibitor of axonal growth in the extracellular matrix of the mature central nervous system. In immunohistochemical and in situ hybridization experiments we show that this tissue-specific splice variant of versican is predominantly ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:Megasporogenesis in the heterosporous fern Marsilea (Hydropterideae) shows features intermediate between sporogenesis in homosporous ferns and that in heterosporous seed plants. The plastids in the dyads and young spores were associated with tubules 30-35 nm in diameter, probably a specialized form of endoplasmic reti...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1981-10-01 00:00:00
