Abstract:
:Mitogen-activated protein kinases (MAPKs) are conserved regulators of proliferation, differentiation and adaptation in eukaryotic cells. Their activity often involves changes in their subcellular localization, indicating an important role for these spatio-temporal dynamics in signal transmission. A striking model illustrating these dynamics is somatic cell fusion in Neurospora crassa Germinating spores of this fungus rapidly alternate between signal sending and receiving, thereby establishing a cell-cell dialog, which involves the alternating membrane recruitment of the MAPK MAK-2 in both fusion partners. Here, we show that the dynamic translocation of MAK-2 is essential for coordinating the behavior of the fusion partners before physical contact. The activation and function of the kinase strongly correlate with its subcellular localization, indicating a crucial contribution of the MAPK dynamics in establishing regulatory feedback loops, which establish the oscillatory signaling mode. In addition, we provide evidence that MAK-2 not only contributes to cell-cell communication, but also mediates cell-cell fusion. The MAK-2 dynamics significantly differ between these two processes, suggesting a role for the MAPK in switching of the cellular program between communication and fusion.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Serrano A,Illgen J,Brandt U,Thieme N,Letz A,Lichius A,Read ND,Fleißner Adoi
10.1242/jcs.213462subject
Has Abstractpub_date
2018-05-04 00:00:00issue
9eissn
0021-9533issn
1477-9137pii
jcs.213462journal_volume
131pub_type
杂志文章abstract::Ryanodine receptors (RyRs) are expressed on the endoplasmic reticulum of many cells, where they form intracellular Ca2+-release channels that participate in the generation of intracellular Ca2+ signals. Here we report studies on the intracellular localisation and functional properties of transfected RyR1 or RyR3 chann...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-06-15 00:00:00
abstract::Asymmetric dimethylarginine (ADMA) is an inhibitor of nitric oxide production associated with abnormal blood vessel growth and repair, however, the mechanism of action of ADMA is not well understood. We studied the role of exogenous and endogenous ADMA in the regulation of cell motility and actin cytoskeleton in porci...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.002212
更新日期:2007-03-15 00:00:00
abstract::Here we report Drosophila Waharan (Wah), a 170-kD predominantly nuclear protein with two potential human homologues, as a newly identified regulator of endosomal trafficking. Wah is required for neuromuscular-junction development and muscle integrity. In muscles, knockdown of Wah caused novel accumulations of tightly ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.060582
更新日期:2010-07-15 00:00:00
abstract::It has been demonstrated that proteins covalently conjugated to folic acid may be taken up by cells via endocytosis after binding to a folate binding protein (FBP) in the cell membrane. The proteins taken up in this manner remain catalytically active and they may modify physiological processes occurring in the cytosol...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00
abstract::Through serial thin-section analysis of rat mammary epithelial cells, the number of centrioles per cell and their intracellular location were determined. In all developmental stages (e.g. virginal, pregnant, lactation, involution), each epithelial cell contained a single centriole that was located in the apical region...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1984-12-01 00:00:00
abstract::Hormone-induced meiotic maturation of the Xenopus oocyte is regulated by complex changes in protein phosphorylation. It is accompanied by a stimulation in the rate of translation, manifest at the level of polypeptide chain initiation. At laser times in the maturation process, this reflects an increased ability for mRN...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00
abstract::Nonsense-mediated mRNA decay (NMD) is an evolutionarily conserved mRNA surveillance system that degrades mRNA transcripts that harbour a premature translation-termination codon (PTC), thus reducing the synthesis of truncated proteins that would otherwise have deleterious effects. Although extensive research has identi...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.120220
更新日期:2013-06-15 00:00:00
abstract::Homophilic binding of E-cadherins through their ectodomains is fundamental to epithelial cell-cell adhesion. Despite this, E-cadherin ectodomains have evolved differently in the vertebrate and insect lineages. Of the five rod-like, tandemly aligned extracellular cadherin domains of vertebrate E-cadherin, the tip extra...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.189258
更新日期:2016-09-01 00:00:00
abstract::The Rho-family proteins make up a major branch of the Ras superfamily of small GTPases. To date, 22 human genes encoding at least 25 proteins have been described. The best known 'classical' members are RhoA, Rac1 and Cdc42. Highly related isoforms of these three proteins have not been studied as intensively, in part b...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.01118
更新日期:2004-03-15 00:00:00
abstract::Despite observations that the lacrimal gland has been identified as the principal source of dimeric immunoglobulin A (dIgA) in tears, the mechanism used by lacrimal gland acinar cells (LGACs) to transcytose dIgA produced by interstitial plasma cells is not well-characterized. This study identifies a transcytotic pathw...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.088906
更新日期:2011-10-15 00:00:00
abstract::Cells need to sense their mechanical environment during the growth of developing tissues and maintenance of adult tissues. The concept of force-sensing mechanisms that act through cell-cell and cell-matrix adhesions is now well established and accepted. Additionally, it is widely believed that force sensing can be med...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.213496
更新日期:2019-07-30 00:00:00
abstract::Tau is a group of neuronal microtubule-associated proteins that are formed by alternative mRNA splicing and accumulate in neurofibrillary tangles in Alzheimer's disease (AD) brain. Tau plays a key role in regulating microtubule dynamics, axonal transport and neurite outgrowth, and all these functions of tau are modula...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.01558
更新日期:2004-11-15 00:00:00
abstract::8-Hydroxyquinoline (HQ) chelates Mg2+ and Mn2+ and, secondarily, affects the activities of DNA and RNA polymerases. The in vivo effect of HQ has been estimated in Allium cepa L. meristems growing under new growth kinetics in the presence of this agent. HQ (at both 5 X 10(-5) M and 10(-4) M) depressed incorporation of ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1986-02-01 00:00:00
abstract::Proper cellular functioning requires that cellular machinery behave in a spatiotemporally regulated manner in response to global changes in nutrient availability. Mounting evidence suggests that one way this is achieved is through the establishment of physically defined gradients of O-GlcNAcylation (O-linked addition ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.113233
更新日期:2014-05-01 00:00:00
abstract::An important aspect of wound healing is the recruitment of neutrophils to the site of infection or tissue injury. Lumican, an extracellular matrix component belonging to the small leucine rich proteoglycan (SLRP) family, is one of the major keratan sulfate proteoglycans (KSPGs) within the corneal stroma. Increasing ev...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.068221
更新日期:2010-09-01 00:00:00
abstract::The iron-storage protein ferritin was found to be associated with highly purified coated vesicles (CV) from chicken liver. Chicken liver ferritin was morphologically similar to ferritin from horse spleen and could be isolated using a specific anti-ferritin monoclonal antibody. This antibody recognized a 240 X 10(3) Mr...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
abstract::Cells undergo a variety of physiological processes, including division, migration and differentiation, under the influence of endogenous electrical cues, which are generated physiologically and pathologically in the extracellular and sometimes intracellular spaces. These signals are transduced to regulate cell behavio...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.023564
更新日期:2009-12-01 00:00:00
abstract::During early stages of meiotic prophase I the nucleus undergoes considerable reorganization, including the clustering of telomeres, the release of contacts between chromosomes and the nuclear membrane, the reorganization of the nucleolus, and chromatin remodeling. Using a light squashing technique for the analysis of ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03155
更新日期:2006-09-15 00:00:00
abstract::IGFBP3 is a multi-functional protein that has IGF-dependent and IGF-independent actions in cultured cells. Here we show that the IGF binding domain (IBD), nuclear localization signal (NLS) and transactivation domain (TA) are conserved and functional in zebrafish Igfbp3. The in vivo roles of these domains were investig...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.082644
更新日期:2011-06-01 00:00:00
abstract::Glycosylphosphatidylinositol-anchored proteins on the egg surface have been proposed to play a role in gamete fusion on the basis of in vitro experiments. We tested this hypothesis by asking if oocyte GPI-anchored proteins are required for fertilization in vivo. Oocyte-specific knockout mice were created using the Cre...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00430
更新日期:2003-06-01 00:00:00
abstract::The model organism Neurospora crassa undergoes programmed cell death when exposed to staurosporine. Here, we show that staurosporine causes defined changes in cytosolic free Ca(2+) ([Ca(2+)]c) dynamics and a distinct Ca(2+) signature that involves Ca(2+) influx from the external medium and internal Ca(2+) stores. We i...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.152058
更新日期:2014-09-01 00:00:00
abstract::Although the 11.5 kDa Zn(2+)-binding protein (ZnBP, parathymosin-alpha) possesses a functional bipartite nuclear localization signal it was found in most tissues in the cytoplasm. The cultivation of freshly isolated rat hepatocytes for 24 hours under standard conditions was associated with an almost complete transloca...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract::Nematocysts are explosive organelles found in all Cnidaria. Explosion of nematocyst capsules is driven by the high pressure within the capsule formed by the high concentration of poly-gamma-glutamate in the capsule matrix. Poly-gamma-glutamate is a polyanion that binds cations tightly, including the fluorescent cation...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-02-15 00:00:00
abstract::Cells that have been pre-exposed to thermal stress can acquire a transient resistance against the killing effect of a subsequent thermal stress. The cause for this phenomenon, called thermotolerance, seems to be an enhanced resistance of proteins against thermal denaturation and aggregation. This resistance can be exp...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Membrane-bound transferrin-like protein (MTf), a glycosylphosphatidylinositol-anchored protein, is expressed at high levels in many tumors and in several fetal and adult tissues including cartilage and the intestine, as well as in the amyloid plaques of Alzheimer's disease, although its role remains unknown. MTf is on...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00393
更新日期:2003-05-15 00:00:00
abstract::Cells experience mechanical stresses in different physiological and pathological settings. Clathrin-coated structures (CCSs) are sensitive to such perturbations in a way that often results in a mechanical impairment of endocytic budding. Compressive stress is a mechanical perturbation that leads to increased membrane ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.239681
更新日期:2020-09-01 00:00:00
abstract::The binding of GFP-tagged tetracycline repressor (TetR) molecules to chromosomally integrated tetracycline operator (tetO) sequence repeats has been used as a system to study chromosome behaviour microscopically in vivo. We found that these integrated transgenes influence the architecture of yeast interphase nuclei, a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-03-15 00:00:00
abstract::SNAREs such as VAMP, SNAP-25 and syntaxin are essential for intracellular trafficking, but what are their exact molecular roles and how are their interactions with other proteins manifest? Capitalizing on the differential sensitivity of SNAREs to exogenous proteases, we quantified the selective removal of identified S...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00374
更新日期:2003-05-15 00:00:00
abstract::Activation of the yeast cell wall integrity (CWI) pathway induces an adaptive transcriptional programme that is largely dependent on the transcription factor Rlm1 and the mitogen-activated protein kinase (MAPK) Slt2. Upon cell wall stress, the transcription factor Rlm1 is recruited to the promoters of RLM1 and SLT2, a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.180190
更新日期:2016-04-15 00:00:00
abstract::Neurite outgrowth is known as a slow (days) process occurring in nerve cells and neurons during neurotrophin treatment and upon transfer to culture, respectively. Using Y27632, a drug that induces activation of Rac1, a downstream step of the neurotrophin signaling cascade, we have identified a new form of outgrowth, w...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.059634
更新日期:2010-01-15 00:00:00