Abstract:
:Here we report Drosophila Waharan (Wah), a 170-kD predominantly nuclear protein with two potential human homologues, as a newly identified regulator of endosomal trafficking. Wah is required for neuromuscular-junction development and muscle integrity. In muscles, knockdown of Wah caused novel accumulations of tightly packed electron-dense tubules, which we termed 'sausage bodies'. Our data suggest that sausage bodies coincide with sites at which ubiquitylated proteins and a number of endosomal and lysosomal markers co-accumulate. Furthermore, loss of Wah function generated loss of the acidic LysoTracker compartment. Together with data demonstrating that Wah acts earlier in the trafficking pathway than the Escrt-III component Drosophila Shrb (snf7 in Schizosaccharomyces pombe), our results indicate that Wah is essential for endocytic trafficking at the late endosome. Highly unexpected phenotypes result from Wah knockdown, in that the distribution of ubiquitylated cargos and endolysosomal morphologies are affected despite Wah being a predominant nuclear protein. This finding suggests the existence of a relationship between nuclear functions and endolysosomal trafficking. Future studies of Wah function will give us insights into this interesting phenomenon.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Lone M,Kungl T,Koper A,Bottenberg W,Kammerer R,Klein M,Sweeney ST,Auburn RP,O'Kane CJ,Prokop Adoi
10.1242/jcs.060582subject
Has Abstractpub_date
2010-07-15 00:00:00pages
2369-74issue
Pt 14eissn
0021-9533issn
1477-9137pii
jcs.060582journal_volume
123pub_type
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