Abstract：
:Nematocysts are explosive organelles found in all Cnidaria. Explosion of nematocyst capsules is driven by the high pressure within the capsule formed by the high concentration of poly-gamma-glutamate in the capsule matrix. Poly-gamma-glutamate is a polyanion that binds cations tightly, including the fluorescent cationic dyes acridine orange and DAPI (4',6-diamidino-2-phenylindole). We have used acridine orange and DAPI staining to localize poly-gamma-glutamate within capsules and to follow the biosynthesis of poly-gamma-glutamate during capsule formation. The results indicate that poly-gamma-glutamate biosynthesis occurs late in capsule formation after invagination of the tubule and that it is accompanied by swelling of the capsule due to increasing osmotic pressure. The matrix in all four capsule types is homogeneously filled with poly-gamma-glutamate. In vivo this poly-gamma-glutamate is complexed with monovalent cations. In addition, poly-gamma-glutamate is formed within the tubule lumen of stenoteles. We argue that this poly-gamma-glutamate is required to drive the two-step explosion process in stenotele nematocysts.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Szczepanek S,Cikala M,David CNkeywords:
subject
Has Abstractpub_date
2002-02-15 00:00:00pages
745-51issue
Pt 4eissn
0021-9533issn
1477-9137journal_volume
115pub_type
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journal_title：Journal of cell science
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pub_type： 杂志文章
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更新日期：2005-12-15 00:00:00
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pub_type： 杂志文章
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更新日期：2007-05-15 00:00:00
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journal_title：Journal of cell science
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更新日期：1997-11-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2012-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1987-04-01 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：1996-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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