RNA trafficking and stabilization elements associate with multiple brain proteins.

abstract：:Integral membrane and secretory proteins which fail to fold productively are retained in the endoplasmic reticulum and targeted for degradation by cytoplasmic proteasomes. Genetic and biochemical analyses suggest that substrates of this pathway must be dislocated across the membrane of the endoplasmic reticulum (ER) b...

journal_title：Journal of cell science

authors： Lenk U,Yu H,Walter J,Gelman MS,Hartmann E,Kopito RR,Sommer T

更新日期：2002-07-15 00:00:00

abstract：:Different isoforms of non-muscle myosin II have different distributions in vivo, even within individual cells. In order to understand how these different distributions arise, the distribution and dynamics of non-muscle myosins IIA and myosin IIB were examined in cultured cells using immunofluorescence staining and tim...

journal_title：Journal of cell science

authors： Kolega J

更新日期：1998-08-01 00:00:00

abstract：:Tritiated-thymidine autoradiography was used to follow the progress of cells through meiosis in male P. cinereus at 20 degrees C. Spermatocytes spend 7 days in leptotene, 5 days in zygotene and 3 days in pachytene before entering the diffuse stage. Diffuse lasts for 8 days and is followed by a diplotene of 2 days. Fir...

journal_title：Journal of cell science

authors： Morgan GT

更新日期：1979-08-01 00:00:00

abstract：:We have generated novel lentiviral vectors to integrate various connexin cDNAs into primary, non-dividing cells. We have used these vectors to test whether proper control of insulin secretion depends on a specific connexin isoform and/or on its level of expression. We have observed that transduced connexin32, connexin...

journal_title：Journal of cell science

authors： Caton D,Calabrese A,Mas C,Serre-Beinier V,Charollais A,Caille D,Zufferey R,Trono D,Meda P

更新日期：2003-06-01 00:00:00

abstract：:We have previously reported that foetal and adult fibroblasts display distinctive migratory phenotypes when cultured on three-dimensional collagen gels. Both skin and tumour-derived fibroblasts from a significant proportion of patients with breast cancer were subsequently observed to display foetal-like migratory beha...

journal_title：Journal of cell science

authors： Schor SL,Schor AM,Rushton G

更新日期：1988-07-01 00:00:00

abstract：:Direct-current electric fields mediate motility (galvanotaxis) of many cell types. In 3T3 fibroblasts, electric fields increased the proportion, speed and cathodal directionality of motile cells. Analogous to fibroblasts' spontaneous migration, we initially hypothesized that reorientation of microtubule components mod...

journal_title：Journal of cell science

authors： Finkelstein E,Chang W,Chao PH,Gruber D,Minden A,Hung CT,Bulinski JC

更新日期：2004-03-15 00:00:00

abstract：:The mammalian inactive X chromosome (Xi) is a model for facultative heterochromatin. Increased DNA compaction for the Xi, and for facultative heterochromatin in general, has long been assumed based on recognition of a distinct Barr body using nucleic-acid staining. This conclusion has been challenged by a report revea...

journal_title：Journal of cell science

authors： Rego A,Sinclair PB,Tao W,Kireev I,Belmont AS

更新日期：2008-04-01 00:00:00

abstract：:Peritubular cells from 15- and 25-day-old rat testis trapped in collagen lattices caused those lattices to contract. Contraction proceeded more rapidly and to a greater extent using cells from younger rats. When 36,000 cells from 15- and 25-day-old rats were trapped in 800 mm2 lattices, the areas were reduced to 28 mm...

journal_title：Journal of cell science

authors： Ehrlich HP,Borland KM,Muffly KE,Hall PF

更新日期：1986-06-01 00:00:00

abstract：:The constitutive (or default) pathway for protein secretion was investigated in two epithelial cells, Madin-Darby canine kidney (MDCK) and human colonic adenocarcinoma (Caco-2), using a bacterial enzyme. The choice of a bacterial protein was based on the requirement to identify a protein devoid of sorting signals. The...

journal_title：Journal of cell science

authors： Soole KL,Hall J,Jepson MA,Hazlewood GP,Gilbert HJ,Hirst BH

更新日期：1992-07-01 00:00:00

abstract：:During meiotic prophase in the ciliate Tetrahymena thermophila micronuclei dramatically elongate and form thread-like crescents. The arrangement of the chromosomes within the crescent as well as the timing of chromosome pairing and recombination with respect to the elongation process have been subjects of ongoing deba...

journal_title：Journal of cell science

authors： Loidl J,Scherthan H

更新日期：2004-11-15 00:00:00

abstract：:Although membrane addition is crucial for cytokinesis in many animal cell types, the specific mechanisms supporting cleavage furrow ingression are not yet understood. Mutations in the gene brunelleschi (bru), which encodes the Drosophila ortholog of the yeast Trs120p subunit of TRAPPII, cause failure of furrow ingress...

journal_title：Journal of cell science

authors： Robinett CC,Giansanti MG,Gatti M,Fuller MT

更新日期：2009-12-15 00:00:00

abstract：:Spatially separated apical and basolateral plasma membrane domains that have distinct functions and molecular compositions are a characteristic feature of epithelial cell polarity. The subapical compartment (SAC), also known as the common endosome (CE), where endocytic pathways from both surfaces merge, plays a crucia...

journal_title：Journal of cell science

authors： Hoekstra D,Tyteca D,van IJzendoorn SC

更新日期：2004-05-01 00:00:00

abstract：:The extracellular environment and tissue architecture contribute to proper cell function and growth control. Cells growing in monolayers on standard polystyrene tissue culture plates differ in their shape, growth rate and response to external stimuli, compared with cells growing in vivo. Here, we showed that the EGFR ...

journal_title：Journal of cell science

authors： Mizushima H,Wang X,Miyamoto S,Mekada E

更新日期：2009-12-01 00:00:00

abstract：:Melanotransferrin (p97) is an iron-binding membrane glycoprotein with 40% homology to transferrin and lactoferrin. It was first identified on the basis of its high level of expression in melanoma cells, as compared to normal melanocytes. It is also present in many cultured cell types. In normal tissues, p97 is express...

journal_title：Journal of cell science

authors： Alemany R,Vilá MR,Francí C,Egea G,Real FX,Thomson TM

更新日期：1993-04-01 00:00:00

abstract：:The monoclonal antibody Bx63 recognizes a centrosomal antigen of Drosophila melanogaster by indirect immunofluorescence and identifies two proteins, with apparent molecular weights of 185 x 10(3) and 66 x 10(3), on Western blots. We have used this antibody to isolate five clones (lambda cs1, -2, -3, -4 and lambda j63)...

journal_title：Journal of cell science

authors： Whitfield WG,Millar SE,Saumweber H,Frasch M,Glover DM

更新日期：1988-04-01 00:00:00

abstract：:An essential step in mammalian fertilisation is the sperm acrosome reaction (AR) - exocytosis of a single large vesicle (the acrosome) that surrounds the nucleus at the apical sperm head. The acrosomal and plasma membranes fuse, resulting in both the release of factors that might facilitate penetration of the zona pel...

journal_title：Journal of cell science

authors： Harper CV,Cummerson JA,White MR,Publicover SJ,Johnson PM

更新日期：2008-07-01 00:00:00

abstract：:The Down syndrome critical region (DSCR) on Chromosome 21 contains many genes whose duplication may lead to the major phenotypic features of Down syndrome and especially the associated mental retardation. However, the functions of DSCR genes are mostly unknown and their possible involvement in key brain developmental ...

journal_title：Journal of cell science

authors： Berto G,Camera P,Fusco C,Imarisio S,Ambrogio C,Chiarle R,Silengo L,Di Cunto F

更新日期：2007-06-01 00:00:00

abstract：:Exocytosis and membrane recycling at the cytoproct (cell anus) of Tetrahymena pyriformis were studied using thin-section electron microscopy. Single cells were fixed at specific times relative to the elimination of the vacuole's contents--before elimination, at elimination, 3--5 s and 10--15 s following elimination. T...

journal_title：Journal of cell science

authors： Allen RD,Wolf RW

更新日期：1979-02-01 00:00:00

abstract：:We have recently shown that leukotriene D(4) (LTD(4)) increases cell survival in intestinal epithelial cells. Here we report and explore the complementary finding that LTD(4) also enhances proliferation in these cells. This proliferative response was approximately half of that induced by epidermal growth factor (EGF) ...

journal_title：Journal of cell science

authors： Paruchuri S,Hallberg B,Juhas M,Larsson C,Sjölander A

更新日期：2002-05-01 00:00:00

abstract：:Recently, several 3D images of kinesin-family motor domains interacting with microtubules have been obtained by analysis of electron microscope images of frozen hydrated complexes at much higher resolutions (9-12 A) than in previous reports (15-30 A). The high-resolution maps show a complex interaction interface betwe...

journal_title：Journal of cell science

authors： Amos LA,Hirose K

更新日期：2007-11-15 00:00:00

abstract：:The change of the nuclear pore frequency during the nuclear cycle of synchronous plasmodia of Physaru- polycephalum was investigated. Counts were made on platinum-carbon replicas of isolated nuclei. Pore numbers varied markedly at any given time of the nuclear cycle, possibly due to the variable DNA contents of the nu...

journal_title：Journal of cell science

authors： Schel JH,Steenbergen LC,Bekers AG,Wanka F

更新日期：1978-12-01 00:00:00

abstract：:Sox7, Sox17 and Sox18 constitute group F of the Sox family of HMG box transcription factor genes. Dominant-negative mutations in Sox18 underlie the cardiovascular defects observed in ragged mutant mice. By contrast, Sox18(-/-) mice are viable and fertile, and display no appreciable anomaly in their vasculature, sugges...

journal_title：Journal of cell science

authors： Matsui T,Kanai-Azuma M,Hara K,Matoba S,Hiramatsu R,Kawakami H,Kurohmaru M,Koopman P,Kanai Y

更新日期：2006-09-01 00:00:00

abstract：:HEK293 cells expressing wild-type CXCR2 recruit PH-Akt-GFP to the leading edge of the cell in response to chemokine. However, in cells expressing mutant CXCR2 defective in AP-2 and HIP binding, i.e. with a mutation in the LLKIL motif, PH-Akt-GFP does not localize to the leading edge in response to ligand. Inhibition o...

journal_title：Journal of cell science

authors： Sai J,Fan GH,Wang D,Richmond A

更新日期：2004-11-01 00:00:00

abstract：:Annexins are cytosolic phospholipid-binding proteins that can be found on the outer leaflet of the plasma membrane. The extracellular functions of annexin include modulating fibrinolysis activity and cell migration. Despite having well-described extracellular functions, the mechanism of annexin transport from the cyto...

journal_title：Journal of cell science

authors： Stewart SE,Ashkenazi A,Williamson A,Rubinsztein DC,Moreau K

更新日期：2018-07-19 00:00:00

abstract：:In invertebrates, UNC-45 regulates myosin stability and functions. Vertebrates have two distinct isoforms of the protein: UNC-45B, expressed in muscle cells only, and UNC-45A, expressed in all cells and implicated in regulating both non-muscle myosin II (NMII)- and microtubule (MT)-associated functions. Here, we show ...

journal_title：Journal of cell science

authors： Habicht J,Mooneyham A,Hoshino A,Shetty M,Zhang X,Emmings E,Yang Q,Coombes C,Gardner MK,Bazzaro M

更新日期：2021-01-08 00:00:00

abstract：:We uncovered a crucial role for the Aurora kinase A (AURKA)-Twist1 axis in promoting epithelial-to-mesenchymal transition (EMT) and chemoresistance in pancreatic cancer. Twist1 is the first EMT-specific target of AURKA that was identified using an innovative screen. AURKA phosphorylates Twist1 at three sites, which re...

journal_title：Journal of cell science

authors： Wang J,Nikhil K,Viccaro K,Chang L,Jacobsen M,Sandusky G,Shah K

更新日期：2017-03-15 00:00:00

abstract：:Desmocollin 3 (Dsc3) is a transmembrane glycoprotein that belongs to the cadherin family of cell adhesion receptors. Together with desmoglein(s), it forms the transmembrane core of desmosomes, a multiprotein complex involved in cell adhesion, organization of the cytoskeleton, cell sorting and cell signaling. Previous ...

journal_title：Journal of cell science

authors： Den Z,Cheng X,Merched-Sauvage M,Koch PJ

更新日期：2006-02-01 00:00:00

abstract：:The number of human genetic disorders shown to be due to defects in membrane trafficking has greatly increased during the past five years. Defects have been identified in components involved in sorting of cargo into transport carriers, vesicle budding and scission, movement of vesicles along cytoskeletal tracks, as we...

journal_title：Journal of cell science

authors： Olkkonen VM,Ikonen E

更新日期：2006-12-15 00:00:00

abstract：:One of the hallmarks of cancer is the ability of tumor cells to invade surrounding tissues and metastasize. During metastasis, cancer cells degrade the extracellular matrix, which acts as a physical barrier, by developing specialized actin-rich membrane protrusion structures called invadopodia. The formation of invado...

journal_title：Journal of cell science

authors： Goicoechea SM,Zinn A,Awadia SS,Snyder K,Garcia-Mata R

更新日期：2017-03-15 00:00:00

abstract：:The small GTPase Arf4 and the Arf GTPase-activating protein (GAP) ASAP1 cooperatively sequester sensory receptor cargo into transport carriers targeted to primary cilia, but the input that drives Arf4 activation in this process remains unknown. Here, we show, by using frog retinas and recombinant human proteins, that ...

journal_title：Journal of cell science

authors： Wang J,Fresquez T,Kandachar V,Deretic D

更新日期：2017-12-01 00:00:00