Abstract：
:One of the hallmarks of cancer is the ability of tumor cells to invade surrounding tissues and metastasize. During metastasis, cancer cells degrade the extracellular matrix, which acts as a physical barrier, by developing specialized actin-rich membrane protrusion structures called invadopodia. The formation of invadopodia is regulated by Rho GTPases, a family of proteins that regulates the actin cytoskeleton. Here, we describe a novel role for RhoG in the regulation of invadopodia disassembly in human breast cancer cells. Our results show that RhoG and Rac1 have independent and opposite roles in the regulation of invadopodia dynamics. We also show that SGEF (also known as ARHGEF26) is the exchange factor responsible for the activation of RhoG during invadopodia disassembly. When the expression of either RhoG or SGEF is silenced, invadopodia are more stable and have a longer lifetime than in control cells. Our findings also demonstrate that RhoG and SGEF modulate the phosphorylation of paxillin, which plays a key role during invadopodia disassembly. In summary, we have identified a novel signaling pathway involving SGEF, RhoG and paxillin phosphorylation, which functions in the regulation of invadopodia disassembly in breast cancer cells.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Goicoechea SM,Zinn A,Awadia SS,Snyder K,Garcia-Mata Rdoi
10.1242/jcs.195552subject
Has Abstractpub_date
2017-03-15 00:00:00pages
1064-1077issue
6eissn
0021-9533issn
1477-9137pii
jcs.195552journal_volume
130pub_type
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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更新日期：1987-11-01 00:00:00
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journal_title：Journal of cell science
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更新日期：1994-03-01 00:00:00
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journal_title：Journal of cell science
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更新日期：1986-03-01 00:00:00
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journal_title：Journal of cell science
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更新日期：1989-10-01 00:00:00
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journal_title：Journal of cell science
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更新日期：1988-08-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2009-10-01 00:00:00
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更新日期：2002-10-15 00:00:00
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journal_title：Journal of cell science
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更新日期：1996-05-01 00:00:00
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更新日期：2018-03-06 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2003-12-01 00:00:00
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更新日期：2011-08-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-12-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1979-10-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1999-01-01 00:00:00
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更新日期：1979-04-01 00:00:00
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journal_title：Journal of cell science
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doi：
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doi：
更新日期：1978-08-01 00:00:00
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doi：
更新日期：1995-06-01 00:00:00
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journal_title：Journal of cell science
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doi：10.1242/jcs.02380
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journal_title：Journal of cell science
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doi：
更新日期：1998-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1989-02-01 00:00:00
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