Abstract：
:Cytostellin, a approximately 240 kDa phosphoprotein found in all cells examined from human to yeast, is predominantly intranuclear in interphase mammalian cells and undergoes continuous redistribution during the cell cycle. Here, mammalian cytostellin is shown to localize to intranuclear regions enriched with multiple splicing proteins, including spliceosome assembly factor, SC-35. Cytostellin and the splicing proteins also co-localize to discrete foci (called 'dots'), which are distributed throughout the cell during mitosis and part of G1. The cytostellin that is localized to these dots resists extraction by Triton X-100, indicating that it is tightly associated with insoluble cell structures. All immunostainable cytostellin reappears in the nucleus before S-phase. Although cytostellin and the splicing proteins co-localize in interphase and dividing cells, cytostellin is not detected in purified spliceosomes, and it associates with six unidentified proteins, forming a macromolecular complex that is biochemically distinct from the proteins that comprise spliceosomes. This macromolecular complex is detected at constant levels throughout the cell cycle, and the level of cytostellin protein remains constant during the cell cycle. Nevertheless, intranuclear cytostellin immunostaining fluctuates markedly during the cell cycle. The monoclonal antibody (mAb) H5 epitope of cytostellin is 'masked' in serum-starved cells, but 60 minutes after serum stimulation intense cytostellin immunoreactivity appears in the nuclear speckles. This rapid induction of cytostellin immunoreactivity in subnuclear regions enriched with many splicing factors, as well as accumulations of RNA polymerase II (Pol II) transcripts, suggests that cytostellin may have a function related to mRNA biogenesis.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Bregman DB,Du L,Li Y,Ribisi S,Warren SLsubject
Has Abstractpub_date
1994-03-01 00:00:00pages
387-96eissn
0021-9533issn
1477-9137journal_volume
107 ( Pt 3)pub_type
杂志文章abstract：:The cavity systems within chicken erythrocyte nuclei and rat liver nuclei were compared using passive probes of radioactive glycogen and active probes of nuclease-armed-glycogen. The passive probe curves have a form that indicates that they are due to passive occupation of spaces and not due to the effects of a limiti...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1979-06-01 00:00:00
abstract：:Purified nuclei retaining a high degree of ultrastructural integrity were isolated by conventional centrifugation techniques. The cytoplasmic surface of these nuclei was iodinated using lactoperoxidase immobilized onto giant Sepharose beads; thus the outer nuclear membrane and the cytoplasmic surface of nuclear pore c...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1980-06-01 00:00:00
abstract：:During mitosis, kinetochores need to attach to microtubules emanating from spindle poles. Several protein complexes have been shown to mediate the kinetochore-microtubule interaction. However, with the continually growing number of newly identified kinetochore proteins, it is unclear whether all major components of th...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.051912
更新日期：2009-07-15 00:00:00
abstract：:Intracellular pH has been measured by laser microspectrofluorimetry, using the pH-sensitive dyes SNARF-1, SNARF-calcein and SNARF-1-dextran. By this technique it was possible to accurately determine pH in volumes as small as 0.5 x 0.5 x 1 microns 3. The probes were loaded into the cells either by diffusion of their ac...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:Clathrin heavy chain is the structural component of the clathrin triskelion, but unique functions for the two distinct and highly conserved clathrin light chains (CLCa and CLCb, also known as CLTA and CLTB, respectively) have been elusive. Here, we show that following detachment and replating, CLCa is uniquely respons...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.224030
更新日期：2019-05-15 00:00:00
abstract：:The sensory functions of cilia are dependent on the enrichment of cilium-resident proteins. Although it is known that ciliary targeting signals (CTSs) specifically target ciliary proteins to cilia, it is still unclear how CTSs facilitate the entry and retention of cilium-resident proteins at the molecular level. We fo...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.194019
更新日期：2016-10-15 00:00:00
abstract：:A glass neodymium laser was modified to make it possible to produce small lesions of 1-2 micron size with a quantitatively known amount of energy. The 1-06-micron radiation of this laser is sufficiently absorbed by water to work without the additions of dyes. Ciliary arrest in Phragmatopoma gills was produced by an am...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1977-04-01 00:00:00
abstract：:Fine-tuning of NMDA glutamatergic receptor signalling strategically controls crucial brain functions. This process depends on several ligands and modulators, one of which unexpectedly includes the serine protease tissue-type plasminogen activator (tPA). In vitro, tPA increases NMDA-receptor-mediated calcium influx by ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03354
更新日期：2007-02-15 00:00:00
abstract：:An essential step in mammalian fertilisation is the sperm acrosome reaction (AR) - exocytosis of a single large vesicle (the acrosome) that surrounds the nucleus at the apical sperm head. The acrosomal and plasma membranes fuse, resulting in both the release of factors that might facilitate penetration of the zona pel...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.030379
更新日期：2008-07-01 00:00:00
abstract：:Re-epithelialization after skin wounding requires both migration and hyperproliferation of keratinocytes. Laminin-332 is deposited during migration over the provisional matrix. To investigate the function of the laminin-332 binding integrin alpha3beta1 in wound re-epithelialization, we generated Itga3flox/flox; K14-Cr...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.029108
更新日期：2009-01-15 00:00:00
abstract：:Intercellular propagation of signals through connexin32-containing gap junctions is of major importance in physiological processes like nerve activity-dependent glucose mobilization in liver parenchymal cells and enzyme secretion from pancreatic acinar cells. In these cells, as in other organs, more than one type of c...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
abstract：:The Arabidopsis AINTEGUMENTA (ANT) gene, which encodes an APETALA2 (AP2)-like transcription factor, controls plant organ cell number and organ size throughout shoot development. ANT is thus a key factor in the development of plant shoots. Here, we have found that ANT plays an essential role in conferring salt toleranc...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.172072
更新日期：2015-08-01 00:00:00
abstract：:The formation and maturation of membrane carriers that transport cargo from the ER to the Golgi complex involves the sequential action of the coat protein complexes COPII and COPI. Recruitment of COPI to nascent carriers requires activation of ADP-ribosylation factors by a BrefeldinA-sensitive guanine nucleotide excha...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03173
更新日期：2006-09-15 00:00:00
abstract：:After DNA replication, cells condense their chromosomes in order to segregate them during mitosis. The condensation process as well as subsequent segregation requires phosphorylation of histone H3 at serine 10. Histone H3 phosphorylation initiates during G2 in pericentric foci prior to H3 phosphorylation in the chromo...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-02-01 00:00:00
abstract：:According to the hypothesis of contact inhibition of movement, cells in a confluent monolayer are restrained from major overlapping by a directional inhibition of locomotion. This explanation of monolayering proposes that contact between 2 cells locally paralyses the locomotory function, preventing movement in the dir...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1978-04-01 00:00:00
abstract：:We previously reported that, among the three connexins expressed in chick lens, overexpression of connexin (Cx) 45.6, not Cx43 or Cx56, stimulates lens cell differentiation; however, the underlying mechanism responsible for this effect is unclear. Here, we took advantage of naturally occurring loss-of-gap-junction fun...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.000935
更新日期：2007-10-15 00:00:00
abstract：:Cells need to sense their mechanical environment during the growth of developing tissues and maintenance of adult tissues. The concept of force-sensing mechanisms that act through cell-cell and cell-matrix adhesions is now well established and accepted. Additionally, it is widely believed that force sensing can be med...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.213496
更新日期：2019-07-30 00:00:00
abstract：:The lipid phosphate phosphatase-related proteins (LPPRs), also known as plasticity-related genes (PRGs), are classified as a new brain-enriched subclass of the lipid phosphate phosphatase (LPP) superfamily. They induce membrane protrusions, neurite outgrowth or dendritic spine formation in cell lines and primary neuro...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.169789
更新日期：2015-09-01 00:00:00
abstract：:Multinucleated giant cells, characteristic of granulomatous infections, originate from the fusion of macrophages. Using an antibody screening strategy we found that the scavenger receptor CD36 participates in macrophage fusion induced by the cytokines IL-4 and GM-CSF. Our results demonstrate that exposure of phosphati...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.037200
更新日期：2009-02-15 00:00:00
abstract：:The monoclonal antibody Bx63 recognizes a centrosomal antigen of Drosophila melanogaster by indirect immunofluorescence and identifies two proteins, with apparent molecular weights of 185 x 10(3) and 66 x 10(3), on Western blots. We have used this antibody to isolate five clones (lambda cs1, -2, -3, -4 and lambda j63)...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1988-04-01 00:00:00
abstract：:The molecular chaperone Hsp90 has been found ubiquitously as a predominantly cytoplasmic dimer. By interacting with cytoplasmic or nuclear proteins such as pp60v-src or steroid receptors, Hsp90 helps its targets to become competent for full biological activity. Mutational deletion analysis of some properties of chicke...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:Eukaryogenesis - the emergence of eukaryotic cells - represents a pivotal evolutionary event. With a fundamentally more complex cellular plan compared to prokaryotes, eukaryotes are major contributors to most aspects of life on Earth. For decades, we have understood that eukaryotic origins lie within both the Archaea ...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.178566
更新日期：2016-10-15 00:00:00
abstract：:BMP-dependent patterning in the Drosophila melanogaster wing imaginal disc serves as a paradigm to understand how morphogens specify cell fates. The observed profile of the transcriptional response to the graded signal of BMP relies upon two counter-active gradients of pMad and Brinker (Brk). This patterning model is ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.110569
更新日期：2012-12-01 00:00:00
abstract：:In animals and yeast, CLASP proteins are microtubule plus-end tracking proteins (+TIPS) involved in the regulation of microtubule plus-end dynamics and stabilization. Here we show that mutations in the Arabidopsis CLASP homolog result in various plant growth reductions, cell form defects and reduced mitotic activity. ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.024950
更新日期：2007-12-15 00:00:00
abstract：:Fas ligand (FasL), a potent mediator of apoptosis expressed by CTL and NK cells, is sorted into the inner vesicles of secretory lysosomes for release via exosome-like vesicles. Previous studies identified a proline-rich domain in the cytoplasmic tail required for sorting FasL to secretory lysosomes, but the mechanisms...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03315
更新日期：2007-01-01 00:00:00
abstract：:Nopp44/46 is a phosphoprotein of the protozoan parasite Trypanosoma brucei that is localized to the nucleolus. Based on the primary sequence, Nopp44/46 appears to be a protein composed of distinct domains. This communication describes the relationship of these domains to the known functional interactions of the molecu...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:Annexin A2 (AnxA2) is a multi-functional and -compartmental protein whose subcellular localisation and functions are tightly regulated by its post-translational modifications. AnxA2 and its Tyr23-phosphorylated form (pTyr23AnxA2) are involved in malignant cell transformation, metastasis and angiogenesis. Here, we show...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.173195
更新日期：2016-01-15 00:00:00
abstract：:The nature, intracellular distribution, and role of proteins synthesized during meiotic maturation of mouse oocytes in vitro have been examined. Proteins synthesized during the initial stages of maturation are concentrated within the nucleus (germinal vesicle) and become intimately associated with the condensing chrom...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1976-05-01 00:00:00
abstract：:Mutations in inositol polyphosphate 5-phosphatase E (INPP5E) cause the ciliopathies known as Joubert and MORM syndromes; however, the role of INPP5E in ciliary biology is not well understood. Here, we describe an interaction between INPP5E and AURKA, a centrosomal kinase that regulates mitosis and ciliary disassembly,...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.161323
更新日期：2015-01-15 00:00:00
abstract：:Super-resolution fluorescence imaging by single-molecule photoactivation or photoswitching and position determination (localization microscopy) has the potential to fundamentally revolutionize our understanding of how cellular function is encoded at the molecular level. Among all powerful, high-resolution imaging tech...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.123612
更新日期：2013-08-15 00:00:00
