Abstract:
:Nopp44/46 is a phosphoprotein of the protozoan parasite Trypanosoma brucei that is localized to the nucleolus. Based on the primary sequence, Nopp44/46 appears to be a protein composed of distinct domains. This communication describes the relationship of these domains to the known functional interactions of the molecule and suggests that the amino-terminal region defines a novel homology region that functions in nucleolar targeting. We have previously shown that Nopp44/46 is capable of interacting with nucleic acids and associating with a protein kinase. Using in vitro transcription and translation, we now demonstrate that the nucleic acid binding function maps to the carboxy-terminal domain of the molecule, a region rich in arginine-glycine-glycine motifs. Our experiments reveal that a central region containing a high proportion of acidic residues is required for association with the protein kinase. Analysis of transfectants expressing epitope-tagged Nopp44/46 deletion constructs showed that the amino-terminal 96 amino acids allowed nuclear and nucleolar accumulation of the protein. This region of the molecule shows homology to several recently described nucleolar proteins. Deletion of a 27-amino-acid region within this domain abrogated nucleolar, but not nuclear, localization. These studies show that Nopp44/46 is composed of distinct modules, each of which plays a different role in molecular interactions. We suggest that this protein could facilitate interactions between sets of nucleolar molecules.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Das A,Park JH,Hagen CB,Parsons Msubject
Has Abstractpub_date
1998-09-01 00:00:00pages
2615-23eissn
0021-9533issn
1477-9137journal_volume
111 ( Pt 17)pub_type
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pub_type: 杂志文章
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更新日期:2009-02-01 00:00:00
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journal_title:Journal of cell science
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更新日期:1998-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-01-15 00:00:00
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更新日期:1980-06-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2017-03-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
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journal_title:Journal of cell science
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更新日期:1983-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-09-01 00:00:00
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journal_title:Journal of cell science
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更新日期:2003-08-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2000-02-01 00:00:00
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更新日期:2017-04-15 00:00:00