Rab27a-mediated protease release regulates neutrophil recruitment by allowing uropod detachment.

abstract：:The distribution of beta 1,2 N-acetylglucosaminyltransferase I (NAGT I), alpha 1,3-1,6 mannosidase II (Mann II), beta 1,4 galactosyltransferase (GalT), alpha 2,6 sialyltransferase (SialylT) was determined by immuno-labelling of cryo-sections from HeLa cell lines. Antibody labelling in the HeLa cell line was made possi...

journal_title：Journal of cell science

authors： Rabouille C,Hui N,Hunte F,Kieckbusch R,Berger EG,Warren G,Nilsson T

更新日期：1995-04-01 00:00:00

abstract：:Fe-S clusters act as co-factors of proteins with diverse functions, for example, in DNA repair. Downregulation of the cytosolic iron-sulfur protein assembly (CIA) machinery promotes genomic instability through the inactivation of multiple DNA repair pathways. Furthermore, CIA deficiencies are associated with so far un...

journal_title：Journal of cell science

authors： Ben-Shimon L,Paul VD,David-Kadoch G,Volpe M,Stümpfig M,Bill E,Mühlenhoff U,Lill R,Ben-Aroya S

更新日期：2018-06-27 00:00:00

abstract：:We have applied fluorescence ratio imaging to the analysis of an actin-binding protein concentration relative to F-actin in macrophages, in order to explore the role of a novel (alpha)-actinin isoform, actinin-4, relative to that of the classical isoform, actinin-1. Conventional immunofluorescence images showed that b...

journal_title：Journal of cell science

authors： Araki N,Hatae T,Yamada T,Hirohashi S

更新日期：2000-09-01 00:00:00

abstract：:The formation and extension of filopodia in response to an extracellular stimulus by guidance cues determine the path of growth cone advance. Actin-filament bundling and actin polymerization at the tips supply the driving force behind the formation and elongation. We tried to clarify how signals in response to extrace...

journal_title：Journal of cell science

authors： Nozumi M,Nakagawa H,Miki H,Takenawa T,Miyamoto S

更新日期：2003-01-15 00:00:00

abstract：:Exocytotic incorporation of plasmalemmal precursor vesicles (PPVs) into the cell surface is necessary for axonal outgrowth and is known to occur mainly at the nerve growth cone. We have demonstrated recently that plasmalemmal expansion is regulated at the growth cone by IGF-1, but not by BDNF, in a manner that is quas...

journal_title：Journal of cell science

authors： Laurino L,Wang XX,de la Houssaye BA,Sosa L,Dupraz S,Cáceres A,Pfenninger KH,Quiroga S

更新日期：2005-08-15 00:00:00

abstract：:Although it is well established that embryonic stem (ES) cells have the potential to differentiate into dopamine neurons, the molecular basis of this process, particularly the role of microRNAs (miRNAs), remains largely unknown. Here we report that miR-132 plays a key role in the differentiation of dopamine neurons by...

journal_title：Journal of cell science

authors： Yang D,Li T,Wang Y,Tang Y,Cui H,Tang Y,Zhang X,Chen D,Shen N,Le W

更新日期：2012-04-01 00:00:00

abstract：:The developmental stage at which Dictyostelium discoideum strain v12/M2 cells was inhibited in resuming cell division was monitored by a Coulter counter. Neither vegetative nor pre-aggregating v12/M2 amoebae used as inocula in liquid growth cultures exhibited a delay in recommencing cell division. Inocula prepared fro...

journal_title：Journal of cell science

authors： Kopachik WJ,Gregg JH

更新日期：1978-02-01 00:00:00

abstract：:Exocytosis and membrane recycling at the cytoproct (cell anus) of Tetrahymena pyriformis were studied using thin-section electron microscopy. Single cells were fixed at specific times relative to the elimination of the vacuole's contents--before elimination, at elimination, 3--5 s and 10--15 s following elimination. T...

journal_title：Journal of cell science

authors： Allen RD,Wolf RW

更新日期：1979-02-01 00:00:00

abstract：:Pregnancy-associated plasma protein A2 (PAPP-A2, also known as pappalysin-2) is a large metalloproteinase that is known to be required for normal postnatal growth and bone development in mice. We here report the detection of zebrafish papp-a2 mRNA in the chordamesoderm, notochord and lower jaw of zebrafish (Danio reri...

journal_title：Journal of cell science

authors： Kjaer-Sorensen K,Engholm DH,Jepsen MR,Morch MG,Weyer K,Hefting LL,Skov LL,Laursen LS,Oxvig C

更新日期：2014-12-01 00:00:00

abstract：:Hematopoietic and vascular endothelial cells constitute the circulatory system and are both generated from the ventral mesoderm. However, the molecules and signaling pathways involved in ventral mesoderm formation and specification remain unclear. We found that zebrafish etv5a was expressed in the ventral mesoderm dur...

journal_title：Journal of cell science

authors： Chen SY,Shih HY,Lin SJ,Hsiao CD,Li ZC,Cheng YC

更新日期：2013-12-15 00:00:00

abstract：:Barrier junctions prevent pathogen invasion and restrict paracellular leakage across epithelial sheets. To understand how one barrier junction, the septate junction (SJ), is regulated in vivo, we used fluorescence recovery after photobleaching (FRAP) to examine SJ protein dynamics in Drosophila. Most SJ-associated pro...

journal_title：Journal of cell science

authors： Oshima K,Fehon RG

更新日期：2011-08-15 00:00:00

abstract：:The protein import machinery of the peroxisome consists of many proteins, collectively called the peroxins. By applying the split-ubiquitin technique we systematically tested the pair-wise interactions between the Nub- and Cub-labeled peroxins for the first time in the living cells of the yeast Saccharomyces cerevisia...

journal_title：Journal of cell science

authors： Eckert JH,Johnsson N

更新日期：2003-09-01 00:00:00

abstract：:Freeze-fracture of fixed and unfixed tissue, lanthanum tracer and conventional thin-section studies have revealed 2 new types of septate junction in the class Anthozoa, phylum Coelenterata. These new junctions have the 15-18-nm intercellular spacing of all other described septate junctions and are found around the api...

journal_title：Journal of cell science

authors： Green CR,Flower NE

更新日期：1980-04-01 00:00:00

abstract：:The interaction of promastigotes of the protozoan parasite Leishmania tropica with mouse peritoneal macrophages in vitro was studied by scanning electron microscopy. Motile promastigotes attached to host cells by their flagellar tips to which the macrophages responded by producing rather closely fitting lamellar sheat...

journal_title：Journal of cell science

authors： Zenian A,Rowles P,Gingell D

更新日期：1979-10-01 00:00:00

abstract：:The mitochondrial influx and efflux of Ca(2+) play a relevant role in cytosolic and mitochondrial Ca(2+) homeostasis, and contribute to the regulation of mitochondrial functions in neurons. The mitochondrial Na(+)/Ca(2+) exchanger, which was first postulated in 1974, has been primarily investigated only from a functio...

journal_title：Journal of cell science

authors： Scorziello A,Savoia C,Sisalli MJ,Adornetto A,Secondo A,Boscia F,Esposito A,Polishchuk EV,Polishchuk RS,Molinaro P,Carlucci A,Lignitto L,Di Renzo G,Feliciello A,Annunziato L

更新日期：2013-12-15 00:00:00

abstract：:Atherosclerosis depends on risk factors such as hyperlipidemia, smoking, hypertension and diabetes. Although these risk factors are relatively constant throughout the arterial circulation, atherosclerotic plaques occur at specific sites where flow patterns are disturbed, with lower overall magnitude and complex change...

journal_title：Journal of cell science

authors： Conway DE,Schwartz MA

更新日期：2013-11-15 00:00:00

abstract：:Reactive oxygen species (ROS) produced by the NADPH oxidase (NOX) complex play important physiological and pathological roles in neurotransmission and neurodegeneration, respectively. However, the contribution of ROS to the molecular mechanisms involved in neuronal polarity and axon elongation is not well understood. ...

journal_title：Journal of cell science

authors： Wilson C,Núñez MT,González-Billault C

更新日期：2015-08-15 00:00:00

abstract：:A complex network of interactions between the stroma, the extracellular matrix and the epithelium drives mammary gland development and function. Two main assumptions in chemical carcinogenesis of the mammary gland have been that carcinogens induce neoplasia by causing mutations in the DNA of the epithelial cells and t...

journal_title：Journal of cell science

authors： Maffini MV,Soto AM,Calabro JM,Ucci AA,Sonnenschein C

更新日期：2004-03-15 00:00:00

abstract：:The translationally controlled tumor protein (TCTP; also known as TPT1 in mammals) is highly conserved and ubiquitously expressed in eukaryotes. It is involved in growth and development, cell cycle progression, protection against cellular stresses and apoptosis, indicating the multifunctional role of the protein. Here...

journal_title：Journal of cell science

authors： Jojic B,Amodeo S,Bregy I,Ochsenreiter T

更新日期：2018-05-10 00:00:00

abstract：:Apical and basolateral endocytic pathways in polarised Caco-2 cells were investigated by following the uptake, recycling and transcytosis of the galactose-binding protein toxin ricin, as a membrane marker. Differences in the extent and kinetics of lectin uptake, recycling and transcytosis were observed at the apical a...

journal_title：Journal of cell science

authors： Jackman MR,Shurety W,Ellis JA,Luzio JP

更新日期：1994-09-01 00:00:00

abstract：:The extracellular matrix molecule tenascin-R (TN-R) and the proteoglycans of the lectican family show an overlapping distribution in the developing brain, have been implicated in similar cellular processes and form a complex network of interactions. Previously, we have demonstrated that TN-R induces microprocesses alo...

journal_title：Journal of cell science

authors： Zacharias U,Rauch U

更新日期：2006-08-15 00:00:00

abstract：:How ion channels localize and distribute on the cell membrane remains incompletely understood. We show that interventions that vary cell adhesion proteins and cell size also affect the membrane current density of inward-rectifier K+ channels (Kir2.1; encoded by KCNJ2) and profoundly alter the action potential shape of...

journal_title：Journal of cell science

authors： Sengupta S,Rothenberg KE,Li H,Hoffman BD,Bursac N

更新日期：2019-09-02 00:00:00

abstract：:How cell fate becomes restricted during somatic cell differentiation is a long-lasting question in biology. Epigenetic mechanisms not present in pluripotent cells and acquired during embryonic development are expected to stabilize the differentiated state of somatic cells and thereby restrict their ability to convert ...

journal_title：Journal of cell science

authors： Pasque V,Radzisheuskaya A,Gillich A,Halley-Stott RP,Panamarova M,Zernicka-Goetz M,Surani MA,Silva JC

更新日期：2012-12-15 00:00:00

abstract：:Formation of crossovers between homologous chromosomes during meiosis is positively regulated by the ZMM proteins (also known as SIC proteins). DNA damage checkpoint proteins also promote efficient formation of interhomolog crossovers. Here, we examined, in budding yeast, the meiotic role of the heterotrimeric DNA dam...

journal_title：Journal of cell science

authors： Shinohara M,Hayashihara K,Grubb JT,Bishop DK,Shinohara A

更新日期：2015-04-15 00:00:00

abstract：:Endothelial cell-based angiogenesis requires activation of survival signals that generate resistance to external apoptotic stimuli, such as tumor necrosis factor-alpha (TNF-alpha), during pathobiologic settings. Mechanisms by which this is achieved are not fully defined. Here, we use a model in which the multifunction...

journal_title：Journal of cell science

authors： Kang-Decker N,Cao S,Chatterjee S,Yao J,Egan LJ,Semela D,Mukhopadhyay D,Shah V

更新日期：2007-02-01 00:00:00

abstract：:A correlation between intranuclear distribution of large tumor antigen (T-ag) and proliferative activity in Simian virus 40 (SV40)-transformed cells has been established. Nuclear T-ag was cytochemically detected by the immunoperoxidase reaction. Cell populations with a high growth rate displayed a heterogeneous patter...

journal_title：Journal of cell science

authors： Ordenes GE,Beck I,Carmona MT,Santos M

更新日期：1989-07-01 00:00:00

abstract：:Calnexin and calreticulin are related proteins that comprise an ER chaperone system that ensures the proper folding and quality control of newly synthesized glycoproteins. The specificity for glycoproteins is conferred by a lectin site that recognizes an early oligosaccharide processing intermediate on the folding gly...

journal_title：Journal of cell science

authors： Williams DB

更新日期：2006-02-15 00:00:00

abstract：:CDC6, a replication licensing protein, is partially exported to the cytoplasm in human cells through phosphorylation by Cdk during S phase, but a significant proportion remains in the nucleus. We report here that human CDC6 physically interacts with ATR, a crucial checkpoint kinase, in a manner that is stimulated by p...

journal_title：Journal of cell science

authors： Yoshida K,Sugimoto N,Iwahori S,Yugawa T,Narisawa-Saito M,Kiyono T,Fujita M

更新日期：2010-01-15 00:00:00

abstract：:A conjugate of horseradish peroxidase (HRP) to poly(L-lysine) (PLL) was used as a non-specific adsorptive probe to study transcytosis in MDCK strain I and Caco-2 epithelial cells. As we have shown previously, HRP-PLL transcytosis proceeds via an intracellular, non-lysosomal proteolytic compartment in MDCK cells; yet, ...

journal_title：Journal of cell science

authors： Taub ME,Shen WC

更新日期：1993-12-01 00:00:00

abstract：:Dynamins are a conserved family of proteins involved in membrane fusion and fission. Although mammalian dynamins are known to be involved in several membrane-trafficking events, the role of dynamin-1 in endocytosis is the best-characterised role of this protein family. Despite many similarities between endocytosis in ...

journal_title：Journal of cell science

authors： Smaczynska-de Rooij II,Allwood EG,Aghamohammadzadeh S,Hettema EH,Goldberg MW,Ayscough KR

更新日期：2010-10-15 00:00:00