Abstract:
:Exocytosis and membrane recycling at the cytoproct (cell anus) of Tetrahymena pyriformis were studied using thin-section electron microscopy. Single cells were fixed at specific times relative to the elimination of the vacuole's contents--before elimination, at elimination, 3--5 s and 10--15 s following elimination. The closed cytoproct is distinguished from other pellicular regions by a single membrane at the cell surface which is circumscribed by an electron-opaque flange that links or welds the plasma membrane to the underlying alveolar margins. Microtubules originating in the flange pass inward where they lie over, and possibly guide, the approaching food vacuoles to the cytoproct. Food facuoles near the cytoproct are also accompanied by coats of microfilaments. These microfilaments appear to be active in the channelling and endocytosis of food vacuole membrane. Upon cytoproct opening the plasma membrane and food vacuole membrane fuse. Elimination seems to be essentially passive and is accomplished by re-engulfment of the old food vacuole membrane which is constantly associated with microfilaments. Reengulfment of all the food vacuole membrane requires 10--15 s and results in a closed cytoproct. The membrane remnants embedded in microfilaments form a cluster under the closed cytoproct. At the periphery of this cluster remnants take the shape of 70--130-nm spherical vesicles or 0.2-micrometer-long flattened vesicles.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Allen RD,Wolf RWkeywords:
subject
Has Abstractpub_date
1979-02-01 00:00:00pages
217-27eissn
0021-9533issn
1477-9137journal_volume
35pub_type
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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更新日期:1990-10-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1979-06-01 00:00:00
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journal_title:Journal of cell science
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更新日期:2006-08-15 00:00:00
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1975-07-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-06-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2015-02-15 00:00:00
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pub_type: 杂志文章
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更新日期:2007-08-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1991-08-01 00:00:00
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journal_title:Journal of cell science
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doi:10.1242/jcs.03020
更新日期:2006-07-01 00:00:00
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1982-06-01 00:00:00
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