Abstract：
:Successful implantation requires the synchronization of viable embryonic development with endometrial receptivity. The mechanisms allowing for the initiation of crosstalk between the embryo and the endometrium remain elusive; however, recent studies have revealed that there are alterations in endometrial microRNAs (miRs) in women suffering repeated implantation failure and that one of the altered miRs is miR-145. We assessed the role of miR-145 and its target IGF1R, in early implantation. miR-145 overexpression and IGF1R knockdown were achieved in Ishikawa endometrial cells. Quantitative PCR, western blotting and 3'UTR luciferase reporter assays confirmed that IGF1R is a direct target of miR-145 in the endometrium. Attachment of mouse embryos or IGF1-coated beads to endometrial epithelial cells was used to study the effects of altered miR-145 and/or IGF1R expression on early implantation events. miR-145 overexpression or specific reduction of IGF1R impaired attachment in both cases. An IGF1R target protector prevented the miR-145-mediated reduction in IGF1R and reversed the effect of miR-145 overexpression on attachment. The data demonstrate that miR-145 influences embryo attachment by reducing the level of IGF1R in endometrium.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Kang YJ,Lees M,Matthews LC,Kimber SJ,Forbes K,Aplin JDdoi
10.1242/jcs.164004subject
Has Abstractpub_date
2015-02-15 00:00:00pages
804-14issue
4eissn
0021-9533issn
1477-9137pii
jcs.164004journal_volume
128pub_type
杂志文章abstract：:SARA, an early endosomal protein, plays a key role in TGFβ signalling, as it presents SMAD2 and SMAD3 for phosphorylation by the activated TGFβ receptors. Here, we show that ERBIN is a new SARA-interacting protein that can be recruited by SARA to early endosomes. ERBIN was recently shown to bind and segregate phosphor...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.062307
更新日期：2011-10-01 00:00:00
abstract：:Meiosis is characterized by two chromosome segregation rounds (meiosis I and II), which follow a single round of DNA replication, resulting in haploid genome formation. Chromosome reduction occurs at meiosis I. It relies on key structures, such as chiasmata, which are formed by repair of double-strand breaks (DSBs) be...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.152520
更新日期：2014-11-01 00:00:00
abstract：:U937 human promonocytic leukemia cells express PKC isozymes beta 1, beta 2, epsilon and zeta. Indirect immunocytofluorescence using affinity-purified PKC-specific antibodies indicates that each of the endogenous PKC isozymes in U937 cells display a unique compartmentalization within the intact cell. PKC-beta 1 is dist...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
abstract：:The constitutive (or default) pathway for protein secretion was investigated in two epithelial cells, Madin-Darby canine kidney (MDCK) and human colonic adenocarcinoma (Caco-2), using a bacterial enzyme. The choice of a bacterial protein was based on the requirement to identify a protein devoid of sorting signals. The...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.239855
更新日期：2020-01-23 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-10-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1242/jcs.064048
更新日期：2010-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
abstract：:Micrographs were made of free-swimming hamster spermatozoa using a high intensity xenon flash and two-colour darkground illumination - conditions that permit three-dimensional reconstruction of the instantaneous shape of the flagellum. From the waveforms observed, we constructed an account of the three-dimensional kin...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1984-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1987-03-01 00:00:00
abstract：:Quantitative studies on the adhesive properties of transformed cells have yielded inconclusive and sometimes contradictory results. The present investigation has examined adhesive interactions between normal human fibroblasts, established as well as virus-transformed animal cell lines, and human tumour-derived cell li...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1978-10-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.195552
更新日期：2017-03-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：:Chloroquine resistance in the human malaria parasite, Plasmodium falciparum, arises from decreased accumulation of the drug in the ;digestive vacuole' of the parasite, an acidic compartment in which chloroquine exerts its primary toxic effect. It has been proposed that changes in the pH of the digestive vacuole might ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02795
更新日期：2006-03-15 00:00:00
abstract：:A correlation between intranuclear distribution of large tumor antigen (T-ag) and proliferative activity in Simian virus 40 (SV40)-transformed cells has been established. Nuclear T-ag was cytochemically detected by the immunoperoxidase reaction. Cell populations with a high growth rate displayed a heterogeneous patter...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1989-07-01 00:00:00
abstract：:In diverse epithelia, cytoskeletal keratin intermediate filaments (IFs) associated with the cytoplasmic face of intercellular junctional desmosomes. The processes underlying desmosome formation and keratin IF interactions remain unclear. We have examined F9 embryonal carcinoma (EC) cell differentiation as a model for ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1977-08-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2014-05-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2010-12-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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journal_title：Journal of cell science
pub_type： 杂志文章,评审
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更新日期：2009-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.211433
更新日期：2018-06-27 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2021-01-25 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2018-09-20 00:00:00
