A mitotic kinesin-6, Pav-KLP, mediates interdependent cortical reorganization and spindle dynamics in Drosophila embryos.

abstract：:Atherosclerosis depends on risk factors such as hyperlipidemia, smoking, hypertension and diabetes. Although these risk factors are relatively constant throughout the arterial circulation, atherosclerotic plaques occur at specific sites where flow patterns are disturbed, with lower overall magnitude and complex change...

journal_title：Journal of cell science

authors： Conway DE,Schwartz MA

更新日期：2013-11-15 00:00:00

abstract：:The telomeric repeat binding factor 2 (TRF2) specifically recognizes TTAGGG tandem repeats at chromosomal ends. Unexpectedly immunofluorescence studies revealed a prominent nucleolar localization of TRF2 in human cells, which appeared as discrete dots with sizes similar to those present in the nucleoplasm. The TRF2 do...

journal_title：Journal of cell science

authors： Zhang S,Hemmerich P,Grosse F

更新日期：2004-08-01 00:00:00

abstract：:New crystal structures of the kinesin motors differ from previously described motor-ADP atomic models, showing striking changes both in the switch I region near the nucleotide-binding cleft and in the switch II or 'relay' helix at the filament-binding face of the motor. The switch I region, present as a short helix fl...

journal_title：Journal of cell science

authors： Kull FJ,Endow SA

更新日期：2002-01-01 00:00:00

abstract：:Ena/VASP proteins are actin-binding proteins that localize to actin stress fibres, the tips of filopodia and the lamellipodial leading edge. In the past few years, a number of seemingly conflicting studies have confused the Ena/VASP field, pointing to roles for these proteins in both promotion and inhibition of actin-...

journal_title：Journal of cell science

authors： Krause M,Bear JE,Loureiro JJ,Gertler FB

更新日期：2002-12-15 00:00:00

abstract：:Actin-filament disassembly is crucial for actin-based motility, to control filament network architecture and to regenerate subunits for assembly. Here, we examined the roles of three actin cytoskeletal proteins, coronin, cofilin and Aip1, which have been suggested to combine in various ways to control actin dynamics b...

journal_title：Journal of cell science

authors： Lin MC,Galletta BJ,Sept D,Cooper JA

更新日期：2010-04-15 00:00:00

abstract：:Galectin-3 is a member of a closely related family of beta-galactoside-binding soluble proteins found in many vertebrate epithelial and myeloid cell types. The developmentally regulated presence of galectin-3 in tissues, for example kidney, and an affinity for many cell-surface and matrix glycoproteins indicate its im...

journal_title：Journal of cell science

authors： Bao Q,Hughes RC

更新日期：1995-08-01 00:00:00

abstract：:Ovarian follicles are the basic functional units of female reproduction in the mammalian ovary. We show here that the protein a disintegrin and metalloproteinase domain 10 (ADAM10), a cell surface sheddase, plays an indispensable role in controlling primordial follicle formation by regulating the recruitment of follic...

journal_title：Journal of cell science

authors： Feng L,Wang Y,Cai H,Sun G,Niu W,Xin Q,Tang X,Zhang J,Wang C,Zhang H,Xia G

更新日期：2016-06-01 00:00:00

abstract：:CDC6, a replication licensing protein, is partially exported to the cytoplasm in human cells through phosphorylation by Cdk during S phase, but a significant proportion remains in the nucleus. We report here that human CDC6 physically interacts with ATR, a crucial checkpoint kinase, in a manner that is stimulated by p...

journal_title：Journal of cell science

authors： Yoshida K,Sugimoto N,Iwahori S,Yugawa T,Narisawa-Saito M,Kiyono T,Fujita M

更新日期：2010-01-15 00:00:00

abstract：:Inhibitory and excitatory neurons exhibit distinct patterns of development in the mammalian cerebral cortex. The morphological development of inhibitory and excitatory neurons derived from fetal rat cerebral cortex has now been compared in vitro. Inhibitory neurons were identified by immunofluorescence staining with a...

journal_title：Journal of cell science

authors： Hayashi K,Kawai-Hirai R,Harada A,Takata K

更新日期：2003-11-01 00:00:00

abstract：:The vacuolar H(+)-ATPase (V-ATPase) is a multi-subunit enzyme that plays important roles in eukaryotic cells. In Dictyostelium, it is found primarily in membranes of the contractile vacuole complex, where it energizes fluid accumulation by this osmoregulatory organelle and also in membranes of endolysosomes, where it ...

journal_title：Journal of cell science

authors： Clarke M,Köhler J,Arana Q,Liu T,Heuser J,Gerisch G

更新日期：2002-07-15 00:00:00

abstract：:The alpha6beta4 integrin is located at the basal surface of keratinocytes, in hemidesmosomal structures that mediate stable adhesion of epidermal cells to the underlying basement membrane component laminin-5. The absence of alpha6beta4 integrin causes junctional epidermolysis bullosa, a severe blistering disease of th...

journal_title：Journal of cell science

authors： Raymond K,Kreft M,Janssen H,Calafat J,Sonnenberg A

更新日期：2005-03-01 00:00:00

abstract：:A fundamental prediction of the hypothesis that critical events in tumour formation are rare is that the tumours develop by clonal expansion. This prediction has been tested by establishing the isozyme content of tumours induced in mosaic animals whose normal tissues contain two distinguishable isozyme activities. The...

journal_title：Journal of cell science

authors： Deamant FD,Iannaccone PM

更新日期：1987-10-01 00:00:00

abstract：:Non-coding RNAs (ncRNAs) are nucleotide sequences that are known to assume regulatory roles previously thought to be reserved for proteins. Their functions include the regulation of protein activity and localization and the organization of subcellular structures. Sequencing studies have now identified thousands of ncR...

journal_title：Journal of cell science

authors： Bensaoud C,Martins LA,Aounallah H,Hackenberg M,Kotsyfakis M

更新日期：2020-11-05 00:00:00

abstract：:p190 RhoGAP is a tyrosine phosphorylated protein that contains an N-terminal GTP binding domain, a middle domain (MD) that mediates interaction with p120 RasGAP and a C-terminal GTPase-activating protein (GAP) domain that is specific for the &Rgr; family of small GTPases. Evidence is accumulating to suggest that p190 ...

journal_title：Journal of cell science

authors： Haskell MD,Nickles AL,Agati JM,Su L,Dukes BD,Parsons SJ

更新日期：2001-05-01 00:00:00

abstract：:MicroRNAs are short non-coding RNAs involved in post-transcriptional regulation of multiple messenger RNA targets. The miR-1/miR-206 family is expressed during skeletal muscle differentiation and is an integral component of myogenesis. To better understand miR-1/miR-206 function during myoblast differentiation we iden...

journal_title：Journal of cell science

authors： Goljanek-Whysall K,Pais H,Rathjen T,Sweetman D,Dalmay T,Münsterberg A

更新日期：2012-08-01 00:00:00

abstract：:During angiogenesis, endothelial cells must coordinate matrix proteolysis with migration. Here, we tested whether the focal adhesion scaffold protein Hic-5 (also known as TGFB1I1) regulated endothelial sprouting in three dimensions. Hic-5 silencing reduced endothelial sprouting and lumen formation, and sprouting defec...

journal_title：Journal of cell science

authors： Dave JM,Abbey CA,Duran CL,Seo H,Johnson GA,Bayless KJ

更新日期：2016-02-15 00:00:00

abstract：:This study uses electron tomography linked to a variety of other EM methods to provide an integrated view of the flagellar pocket and basal body area of the African trypanosome procyclic trypomastigote. We reveal the pocket as an asymmetric membranous 'balloon' with two boundary structures. One of these - the collar -...

journal_title：Journal of cell science

authors： Lacomble S,Vaughan S,Gadelha C,Morphew MK,Shaw MK,McIntosh JR,Gull K

更新日期：2009-04-15 00:00:00

abstract：:When migrating mesenchymal cells collide, they exhibit a 'contact inhibition of locomotion' response that results in reversal of their front-rear polarity by extension of a new leading edge, which enables their migration away from the opposing contacted cell. The critical cytoskeletal rearrangements underpinning these...

journal_title：Journal of cell science

authors： Kadir S,Astin JW,Tahtamouni L,Martin P,Nobes CD

更新日期：2011-08-01 00:00:00

abstract：:During mitosis, kinetochores need to attach to microtubules emanating from spindle poles. Several protein complexes have been shown to mediate the kinetochore-microtubule interaction. However, with the continually growing number of newly identified kinetochore proteins, it is unclear whether all major components of th...

journal_title：Journal of cell science

authors： Raaijmakers JA,Tanenbaum ME,Maia AF,Medema RH

更新日期：2009-07-15 00:00:00

abstract：:Although the 11.5 kDa Zn(2+)-binding protein (ZnBP, parathymosin-alpha) possesses a functional bipartite nuclear localization signal it was found in most tissues in the cytoplasm. The cultivation of freshly isolated rat hepatocytes for 24 hours under standard conditions was associated with an almost complete transloca...

journal_title：Journal of cell science

authors： Trompeter HI,Schiermeyer A,Blankenburg G,Hennig E,Söling HD

更新日期：1999-11-01 00:00:00

abstract：:The hedgehog (Hh) signaling pathway plays a very important role in metazoan development by controlling pattern formation. Malfunction of the Hh signaling pathway leads to numerous serious human diseases, including congenital disorders and cancers. The seven-transmembrane domain protein Smoothened (Smo) is a key transd...

journal_title：Journal of cell science

authors： Yang X,Mao F,Lv X,Zhang Z,Fu L,Lu Y,Wu W,Zhou Z,Zhang L,Zhao Y

更新日期：2013-09-15 00:00:00

abstract：:PACSIN2, a membrane-sculpting BAR domain protein, localizes to caveolae. Here, we found that protein kinase C (PKC) phosphorylates PACSIN2 at serine 313, thereby decreasing its membrane binding and tubulation capacities. Concomitantly, phosphorylation decreased the time span for which caveolae could be tracked at the ...

journal_title：Journal of cell science

authors： Senju Y,Rosenbaum E,Shah C,Hamada-Nakahara S,Itoh Y,Yamamoto K,Hanawa-Suetsugu K,Daumke O,Suetsugu S

更新日期：2015-08-01 00:00:00

abstract：:The ribonucleoprotein enzyme RNase P catalyzes the 5' processing of pre-transfer RNA, and has also recently been implicated in pre-ribosomal RNA processing. In the present investigation, in situ hybridization revealed that RNase P RNA is present throughout the nucleus of mammalian cells. However, rhodamine-labeled hum...

journal_title：Journal of cell science

authors： Jacobson MR,Cao LG,Taneja K,Singer RH,Wang YL,Pederson T

更新日期：1997-04-01 00:00:00

abstract：:To enhance our understanding of the function(s) of gamma-tubulin-complex proteins (GCPs), we identified and analyzed the functions of the Aspergillus nidulans homologs of GCP2-GCP6 (here designated GCPB-GCBF). The gamma-tubulin small complex (gamma-TuSC) components, gamma-tubulin, GCPB and GCPC, are essential for viab...

journal_title：Journal of cell science

authors： Xiong Y,Oakley BR

更新日期：2009-11-15 00:00:00

abstract：:The lepidopteran-specific P1 delta-endotoxin of Bacillus thuringiensis var. kurstaki HD-1 was activated in vitro using insect gut proteases and found to be highly specific for the lepidopteran cell line Choristoneura fumiferana CF1 among a wide range of lepidopteran and dipteran cell lines tested. The toxicity of P1 a...

journal_title：Journal of cell science

authors： Knowles BH,Ellar DJ

更新日期：1986-07-01 00:00:00

abstract：:The C terminus of the tubulin alpha-subunit of most eukaryotic cells undergoes a cycle of tyrosination and detyrosination using two specific enzymes, a tubulin tyrosine ligase (TTL) and a tubulin carboxypeptidase. Although this enzyme cycle is conserved in evolution and exhibits rapid turnover, the meaning of this mod...

journal_title：Journal of cell science

authors： Lafanechère L,Courtay-Cahen C,Kawakami T,Jacrot M,Rüdiger M,Wehland J,Job D,Margolis RL

更新日期：1998-01-01 00:00:00

abstract：:Deleted in liver cancer 1 (DLC1) is a multi-modular Rho-GTPase-activating protein (RhoGAP) and a tumor suppressor. Besides its RhoGAP domain, functions of other domains in DLC1 remain largely unknown. By protein precipitation and mass spectrometry, we identified eukaryotic elongation factor 1A1 (EF1A1) as a novel part...

journal_title：Journal of cell science

authors： Zhong D,Zhang J,Yang S,Soh UJ,Buschdorf JP,Zhou YT,Yang D,Low BC

更新日期：2009-02-01 00:00:00

abstract：:Increases in cytosolic Ca(2+) concentration ([Ca(2+)](c)) mediated by inositol (1,4,5)-trisphosphate [Ins(1,4,5)P(3), hereafter InsP(3)] regulate activities that include division, contraction and cell death. InsP(3)-evoked Ca(2+) release often begins at a single site, then regeneratively propagates through the cell as...

journal_title：Journal of cell science

authors： Olson ML,Sandison ME,Chalmers S,McCarron JG

更新日期：2012-11-15 00:00:00

abstract：:Non-muscle myosin II (MyoII) contractility is central to the regulation of numerous cellular processes, including migration. Rho is a well-characterized modulator of actomyosin contractility, but the function of other GTPases, such as Rac, in regulating contractility is currently not well understood. Here, we show tha...

journal_title：Journal of cell science

authors： Jean L,Majumdar D,Shi M,Hinkle LE,Diggins NL,Ao M,Broussard JA,Evans JC,Choma DP,Webb DJ

更新日期：2013-12-15 00:00:00

abstract：:Super-resolution fluorescence imaging by single-molecule photoactivation or photoswitching and position determination (localization microscopy) has the potential to fundamentally revolutionize our understanding of how cellular function is encoded at the molecular level. Among all powerful, high-resolution imaging tech...

journal_title：Journal of cell science

authors： Sauer M

更新日期：2013-08-15 00:00:00