Abstract：
:Ovarian follicles are the basic functional units of female reproduction in the mammalian ovary. We show here that the protein a disintegrin and metalloproteinase domain 10 (ADAM10), a cell surface sheddase, plays an indispensable role in controlling primordial follicle formation by regulating the recruitment of follicle supporting cells in mice. We demonstrate that suppressing ADAM10 in vitro or deletion of Adam10 in vivo disrupts germline cyst breakdown and primordial follicle formation. Using a cell lineage tracing approach, we show that ADAM10 governs the recruitment of ovarian follicle cells by regulating the differentiation and proliferation of LGR5-positive follicle supporting progenitor cells. By detecting the development of FOXL2-positive pregranulosa cells, we found that inhibiting ADAM10 reduced the number of FOXL2-positive cells in perinatal ovaries. Furthermore, inhibiting ADAM10 suppressed the activation of Notch signaling, and blocking Notch signaling also disrupted the recruitment of follicle progenitor cells. Taken together, these results show that ADAM10-Notch signaling in ovarian somatic cells governs the primordial follicle formation by controlling the development of ovarian pregranulosa cells. The proper recruitment of ovarian follicle supporting cells is essential for establishment of the ovarian reserve in mice.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Feng L,Wang Y,Cai H,Sun G,Niu W,Xin Q,Tang X,Zhang J,Wang C,Zhang H,Xia Gdoi
10.1242/jcs.184267subject
Has Abstractpub_date
2016-06-01 00:00:00pages
2202-12issue
11eissn
0021-9533issn
1477-9137pii
jcs.184267journal_volume
129pub_type
杂志文章abstract：:Pex11 homologs and dynamin-related proteins uniquely regulate peroxisome division (cell-cycle-dependent duplication) and proliferation (cell-cycle-independent multiplication). Arabidopsis plants possess five Pex11 homologs designated in this study as AtPex11a, -b, -c, -d and -e. Transcripts for four isoforms were foun...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02904
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abstract：:Glomerular basement membrane (GBM) and podocalyxin are essential for podocyte morphology. We provide evidence of functional interconnections between basement membrane components (collagen IV and laminin), the expression of podocalyxin and the morphology of human glomerular epithelial cells (podocytes). We demonstrated...
journal_title：Journal of cell science
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journal_title：Journal of cell science
pub_type： 杂志文章
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abstract：:During meiosis, cohesion between sister chromatids is required for normal levels of homologous recombination, maintenance of chiasmata and accurate chromosome segregation during both divisions. In Drosophila, null mutations in the ord gene abolish meiotic cohesion, although how ORD protein promotes cohesion has remain...
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pub_type： 评论,杂志文章,评审
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更新日期：2001-10-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：1999-03-01 00:00:00
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journal_title：Journal of cell science
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doi：
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更新日期：2018-11-05 00:00:00
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journal_title：Journal of cell science
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doi：
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journal_title：Journal of cell science
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更新日期：1997-10-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：2000-01-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2016-07-15 00:00:00
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journal_title：Journal of cell science
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更新日期：1998-01-01 00:00:00
