Abstract:
:The ribonucleoprotein enzyme RNase P catalyzes the 5' processing of pre-transfer RNA, and has also recently been implicated in pre-ribosomal RNA processing. In the present investigation, in situ hybridization revealed that RNase P RNA is present throughout the nucleus of mammalian cells. However, rhodamine-labeled human RNase P RNA microinjected into the nucleus of rat kidney (NRK) epithelial cells or human (HeLa) cells initially localized in nucleoli, and subsequently became more evenly distributed throughout the nucleus, similar to the steadystate distribution of endogenous RNase P RNA. Parallel microinjection and immunocytochemical experiments revealed that initially nucleus-microinjected RNase P RNA localized specifically in the dense fibrillar component of the nucleolus, the site of pre-rRNA processing. A mutant RNase P RNA lacking the To antigen binding domain (nucleotides 25-75) did not localize in nucleoli after nuclear microinjection. In contrast, a truncated RNase P RNA containing the To binding domain but lacking nucleotides 89-341 became rapidly localized in nucleoli following nuclear microinjection. However, unlike the full-length RNase P RNA, this 3' truncated RNA remained stably associated with the nucleoli and did not translocate to the nucleoplasm. These results suggest a nucleolar phase in the maturation, ribonucleoprotein assembly or function of RNase P RNA, mediated at least in part by the nucleolar To antigen. These and other recent findings raise the intriguing possibility of a bifunctional role of RNase P in the nucleus: catalyzing pre-ribosomal RNA processing in the nucleolus and pre-transfer RNA processing in the nucleoplasm.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Jacobson MR,Cao LG,Taneja K,Singer RH,Wang YL,Pederson Tsubject
Has Abstractpub_date
1997-04-01 00:00:00pages
829-37eissn
0021-9533issn
1477-9137journal_volume
110 ( Pt 7)pub_type
杂志文章abstract::An essential step in mammalian fertilisation is the sperm acrosome reaction (AR) - exocytosis of a single large vesicle (the acrosome) that surrounds the nucleus at the apical sperm head. The acrosomal and plasma membranes fuse, resulting in both the release of factors that might facilitate penetration of the zona pel...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.030379
更新日期:2008-07-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2002-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1997-06-01 00:00:00
abstract::To investigate the mechanism of chromatin assembly at human centromeres, we isolated cultured human cell lines in which a transfected alpha-satellite (alphoid) YAC was integrated ectopically into the terminal region of host chromosome 16, where it was stably maintained. Centromere activity of the alphoid YAC was suppr...
journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2003-10-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2020-06-24 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2007-05-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1986-03-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-02-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
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更新日期:2007-01-01 00:00:00
abstract::The tetrapeptide N-Acetyl-Seryl-Aspartyl-Lysyl-Proline (AcSDKP) is a natural regulator of hematopoietic stem cell proliferation. The present study was aimed at investigating the presence and the role of AcSDKP in rat testis. Specific immunoreactivity was always observed in the interstitial tissue at all stages of test...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1991-03-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.009415
更新日期:2007-11-01 00:00:00
abstract::Cell fusion plays a well-recognized, physiological role during development. Bone-marrow-derived hematopoietic cells have been shown to fuse with non-hematopoietic cells in a wide variety of tissues. Some organs appear to resolve the changes in ploidy status, generating functional and mitotically-competent events. Howe...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.100123
更新日期:2012-06-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.151423
更新日期:2015-02-15 00:00:00
abstract::Transcription factor E2F plays an important role in coordinating and integrating early cell cycle progression with the transcription apparatus. It is known that physiological E2F arises when a member of two families of proteins, E2F and DP, interact as E2F/DP heterodimers and that transcriptional activity is regulated...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
abstract::Effective cell-to-cell spreading of the facultative intracellular pathogen Listeria monocytogenes requires the interaction between bacteria and the microfilament system of the host cell. By recruiting actin filaments into a 'comet tail' localized at one pole of the bacterial cell wall, Listeria become mobile and prope...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::Certain embryonic tissue masses and cell aggregates behave like deformable solids during brief experimental manipulations but like viscous liquids in long-term organ cultures. To investigate these seemingly paradoxical physical properties, we have mechanically deformed cell aggregates derived from several embryonic ch...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1978-04-01 00:00:00
abstract::In situ observations of the development of hippocampal and cortical neurons indicate that final axon-dendrite identity is defined at the time of generation of the first two, oppositely positioned, neurites. Quite differently, in vitro studies demonstrated that axonal fate is defined by the stochastic selection of one ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.023143
更新日期:2008-01-15 00:00:00
abstract::Mitotic spindle assembly in animal cells is orchestrated by a chromosome-dependent pathway that directs microtubule stabilization. RanGTP generated at chromosomes releases spindle assembly factors from inhibitory complexes with importins, the nuclear transport factors that facilitate protein import into the nucleus du...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.126854
更新日期:2013-08-01 00:00:00
abstract::Protein kinase CK2, a vital, pleiotropic and highly conserved serine/threonine phosphotransferase is involved in transcription-directed signaling, gene control and cell cycle regulation and is suspected to play a role in global processes. Searching for these global roles, we analyzed the involvement of CK2 in gene exp...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00352
更新日期:2003-04-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01488
更新日期:2004-11-01 00:00:00
abstract::In the fission yeast Schizosaccharomyces pombe, the protein kinase Chk1 has an essential role in transducing a delay signal to the cell cycle machinery in the presence of DNA damage. Fission yeast cells lacking the chk1 gene do not delay progression of the cell cycle in response to damage and are thus sensitive to DNA...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00133
更新日期:2002-12-01 00:00:00
abstract::The mAb RK7, previously shown to recognize keratin 19, was also found to cross-react with a biologically unrelated 102 kDa protein, which becomes associated with the poles of the mitotic apparatus. This newly identified protein, called cytocentrin, is a stable cellular component, may be at least in part phosphorylated...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
abstract::The DNA content of isolated micronuclei, differentiating macronuclei (macronuclear Anlagen), and adult macronuclei of Loxodes magnus was measured cytofluorimetrically in preparations stained with a Schiff-type reagent, auramine-SO2, following hydrochloric acid hydrolysis. The DNA content of the youngest macronuclear A...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::Integrin and receptor tyrosine kinase signalling networks cooperate to regulate various biological functions. The molecular details underlying the integration of both signalling networks remain largely uncharacterized. Here we identify a signalling module composed of a fibronectin-α5β1-integrin-integrin-linked-kinase ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.091652
更新日期:2012-01-15 00:00:00
abstract::High endothelial cells lining the post capillary venules in the paracortical areas of rat lymph nodes were found by autoradiography to incorporate [35S]sulphate, whether it was injected into the footpad to reach the draining popliteal lymph node or added to short-term cultures of cervical lymph node slices. The early ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1982-10-01 00:00:00
abstract::The mechanism by which glutamate regulates the cytosolic free Ca2+ concentration ([Ca2+]c) in spontaneously firing dopamine neurons is not clear. Thus we have investigated the glutamate-mediated [Ca2+]c dynamics in the acutely isolated dopamine neurons from the rat substantia nigra pars compacta by measuring [Ca2+]c a...
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pub_type: 杂志文章
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更新日期:2003-07-01 00:00:00