Abstract：
:Motile eukaryotic flagella beat through coordinated activity of dynein motor proteins; however, the mechanisms of dynein coordination and regulation are incompletely understood. The inner dynein arm (IDA) f complex (also known as the I1 complex), and the tether and tether head (T/TH) complex are thought to be key regulators of dynein action but, unlike the IDA f complex, T/TH proteins remain poorly characterised. Here, we characterised T/TH-associated proteins in the protist Leishmania mexicana Proteome analysis of axonemes from null mutants for the CFAP44 T/TH protein showed that they lacked the IDA f protein IC140 and a novel 28-kDa axonemal protein, LAX28. Sequence analysis identified similarities between LAX28 and the uncharacterised human sperm tail protein TEX47, both sharing features with sensory BLUF-domain-containing proteins. Leishmania lacking LAX28, CFAP44 or IC140 retained some motility, albeit with reduced swimming speed and directionality and a propensity for flagellar curling. Expression of tagged proteins in different null mutant backgrounds showed that the axonemal localisation of LAX28 requires CFAP44 and IC140, and the axonemal localisations of CFAP44 and IC140 both depend on LAX28. These data demonstrate a role for LAX28 in motility and show mutual dependencies of IDA f and T/TH-associated proteins for axonemal assembly in Leishmania.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Beneke T,Banecki K,Fochler S,Gluenz Edoi
10.1242/jcs.239855subject
Has Abstractpub_date
2020-01-23 00:00:00issue
2eissn
0021-9533issn
1477-9137pii
jcs.239855journal_volume
133pub_type
杂志文章abstract：:Thrombospondin-1 (TSP-1) and the highly related protein thrombospondin-2 (TSP-2) are trimeric extracellular molecules that have complex roles in wound healing, angiogenesis and matrix organisation. At the cellular level, TSP-1 supports cell adhesion and migration by the organisation of fascin spike cytoskeletal struct...
journal_title：Journal of cell science
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abstract：:Rolling blackout (RBO) is a Drosophila EFR3 integral membrane lipase. A conditional temperature-sensitive (TS) mutant (rbo(ts)) displays paralysis within minutes following a temperature shift from 25 degrees C to 37 degrees C, an impairment previously attributed solely to blocked synaptic-vesicle exocytosis. However, ...
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更新日期：2009-07-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2007-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1978-08-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.108373
更新日期：2012-11-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1983-03-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1993-03-01 00:00:00
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更新日期：2014-05-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2020-05-18 00:00:00
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journal_title：Journal of cell science
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更新日期：2016-03-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2008-05-15 00:00:00
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doi：
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doi：
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journal_title：Journal of cell science
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