Abstract：
:Retinoic acid (RA) plays key roles in cell differentiation and growth arrest by activating nuclear RA receptors (RARs) (α, β and γ), which are ligand-dependent transcription factors. RARs are also phosphorylated in response to RA. Here, we investigated the in vivo relevance of the phosphorylation of RARs during RA-induced neuronal differentiation of mouse embryonic stem cells (mESCs). Using ESCs where the genes encoding each RAR subtype had been inactivated, and stable rescue lines expressing RARs mutated in phospho-acceptor sites, we show that RA-induced neuronal differentiation involves RARγ2 and requires RARγ2 phosphorylation. By gene expression profiling, we found that the phosphorylated form of RARγ2 regulates a small subset of genes through binding an unusual RA response element consisting of two direct repeats with a seven-base-pair spacer. These new findings suggest an important role for RARγ phosphorylation during cell differentiation and pave the way for further investigations during embryonic development.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Al Tanoury Z,Gaouar S,Piskunov A,Ye T,Urban S,Jost B,Keime C,Davidson I,Dierich A,Rochette-Egly Cdoi
10.1242/jcs.145979subject
Has Abstractpub_date
2014-05-01 00:00:00pages
2095-105issue
Pt 9eissn
0021-9533issn
1477-9137pii
jcs.145979journal_volume
127pub_type
杂志文章abstract：:Trypsin digestion of demembranated fowl spermatozoa caused a longitudinal splitting of the distal part of the axoneme. The resulting strands, consisting of groups of doublet microtubules, formed left-handed helices. On the evidence of electron micrographs, the digestion had caused the loss of dynein arms from the oute...
journal_title：Journal of cell science
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更新日期：1987-10-01 00:00:00
abstract：:In metazoans, cyclin-dependent kinase 7 (CDK7) has essential roles in both the cell-division cycle and transcription, as a CDK-activating kinase (CAK) and as a component of the general transcription factor TFIIH, respectively. Controversy over its double duty has been resolved, but questions remain. First, how does CD...
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journal_title：Journal of cell science
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doi：10.1242/jcs.157610
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abstract：:Several cell types express inducible nitric oxide synthase (NOS2) in response to exogenous insults such as bacterial lipopolysaccharide (LPS) or proinflammatory cytokines. For instance, muscular cells treated with LPS and interferon gamma (IFN-gamma) respond by increasing the mRNA and protein levels of NOS2, and synth...
journal_title：Journal of cell science
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doi：10.1242/jcs.01002
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journal_title：Journal of cell science
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doi：10.1242/jcs.116285
更新日期：2013-04-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1986-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1984-03-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1978-10-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
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journal_title：Journal of cell science
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doi：
更新日期：2002-04-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1987-11-01 00:00:00
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pub_type： 杂志文章,评审
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2005-04-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
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doi：
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journal_title：Journal of cell science
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doi：
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journal_title：Journal of cell science
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doi：
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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