Abstract:
:We previously demonstrated that activation of v-ABL protein tyrosine kinase resulted in suppression of apoptosis following interleukin-3 removal using an interleukin-3-dependent haemopoietic cell line transfected with a temperature-sensitive mutant of the v-abl oncoprotein (IC.DP). Cellular signalling events associated with the activation of v-ABL included increased levels of sn-1,2-diacylglycerol, an activator of protein kinase C. Calphostin C, a PKC inhibitor, restored apoptosis to interleukin-3-deprived IC.DP cells expressing active v-ABL. However, chronic exposure to the phorbol ester, 12-O-tetradecanoyl phorbol 13-acetate to downregulate protein kinase C did not attenuate the survival of IC.DP cells expressing active v-ABL. Translocation of a classical protein kinase C isozyme(s) to the nuclear fraction was observed 6 hours after activation of v-ABL, when nuclear protein kinase C activity was increased approximately 2-fold. The protein kinase C isozyme responsible, which was only partially downregulated by 12-O-tetradecanoyl phorbol 13-acetate, was identified as protein kinase C beta II. This translocation of protein kinase C beta II to the nucleus was inhibited by calphostin C. Taken together, these results suggest that nuclear translocation and activation of PKC beta II may play a role in v-ABL-mediated suppression of apoptosis.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Evans CA,Lord JM,Owen-Lynch PJ,Johnson G,Dive C,Whetton ADsubject
Has Abstractpub_date
1995-07-01 00:00:00pages
2591-8eissn
0021-9533issn
1477-9137journal_volume
108 ( Pt 7)pub_type
杂志文章abstract::A monoclonal antibody, specific to phosphoproteins in mitotic HeLa cells was found to crossreact with a similar set of proteins in embryos of the nematode, Caenorhabditis elegans. In C. elegans, as in mammalian cells, the highly conserved antigenic epitope is associated with a family of high molecular weight polypepti...
journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-03-01 00:00:00
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journal_title:Journal of cell science
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更新日期:2008-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1994-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1993-10-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.004846
更新日期:2007-05-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1991-09-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.101337
更新日期:2012-05-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2017-10-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-05-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01334
更新日期:2004-10-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::The chromosomes of the active previtellogenic oocytes of the dragonfly Cordulia aenea L. extrude into the cytoplasm a substance, which is subsequently found there in the form of granulofibrillar masses. These nuclear extrusions evolve ultrastructurally into two components, nematosomes and dense masses. The nematosomes...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1975-10-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1988-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-07-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
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更新日期:2017-06-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-09-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01631
更新日期:2005-01-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1990-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2021-01-25 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-03-01 00:00:00
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journal_title:Journal of cell science
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更新日期:1994-04-01 00:00:00